Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB122 4,266,097 A→C D147A (GAC→GCC)  CJ019_04001 → putative FAD‑linked oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1224,266,0970AC97.7% 314.5 / ‑2.8 88D147A (GAC→GCC) CJ019_04001putative FAD‑linked oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base C (35/51);  total (36/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

TGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGTCGAGGGCGAAC  >  ACB122/4265965‑4266228
                                                                                                                                    |                                                                                                                                   
tgctgGTTATGTCGCGCTTGAACCGTATCCTCGAGGTCATCCCGCTGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCACCCAGGCCGCCGCACCCCATGGTATGAACTACGCACCCGCCCCt                                                                                                                                 <  5:84879/137‑1 (MQ=255)
tgctgGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCt                                                                                                                                 >  3:415602/1‑137 (MQ=255)
tgctgGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCt                                                                                                                                 >  4:329495/1‑137 (MQ=255)
tgctgGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCAACCAGCAGGGGCGTTACGCCCGCGTGCACCCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCCCCCCATGGTATGTACTACGCACCCGCCCCt                                                                                                                                 >  3:337542/1‑137 (MQ=255)
tgctgGTGATGGCGCGCTTCAACAGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCt                                                                                                                                 <  5:91623/137‑1 (MQ=255)
            cgcgcTTCAACCGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAAtt                                                                                                                     <  3:340982/137‑1 (MQ=255)
               gcTTCAACCGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGcc                                                                                                                  >  3:249688/1‑137 (MQ=255)
                   cAACCGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCt                                                                                                              <  4:60015/137‑1 (MQ=255)
                    aaCCGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTc                                                                                                             <  4:356870/137‑1 (MQ=255)
                            ccTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTg                                                                                                     >  7:152525/1‑137 (MQ=255)
                            ccTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTg                                                                                                     >  2:531/1‑137 (MQ=255)
                              tCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGc                                                                                                   >  7:71567/1‑137 (MQ=255)
                                  ggTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACg                                                                                               <  2:115954/137‑1 (MQ=255)
                                   gTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGt                                                                                              >  4:310003/1‑137 (MQ=255)
                                   gTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGt                                                                                              >  1:294568/1‑137 (MQ=255)
                                       ccccGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGcc                                                                                          <  3:261830/136‑1 (MQ=255)
                                       aCCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGTTATGTACTACGCACCCGCCCCTTCCTCTCAAATTGCCTGCTCGATCGTTGGCAACGTCGcc                                                                                          <  3:116447/137‑1 (MQ=255)
                                       aCCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGcc                                                                                          <  8:225580/137‑1 (MQ=255)
                                            cAGGGGCGGTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGaaaa                                                                                     <  7:305336/137‑1 (MQ=255)
                                              ggggCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACg                                                                                   <  4:415602/137‑1 (MQ=255)
                                              ggggCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACg                                                                                   <  5:118881/137‑1 (MQ=255)
                                                     tACGCCCGCGTGCCACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATTGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCCGTgg                                                                            >  8:167712/1‑137 (MQ=255)
                                                        gCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCTCAAATTGCCTGCTCGATCGGTGGCAACGTCTCCGAAAACGCCGGTTGCGt                                                                         <  6:187358/137‑1 (MQ=255)
                                                        gCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGt                                                                         <  5:49672/137‑1 (MQ=255)
                                                        gCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGGCGCCGAAAACGCCGGTGGCGt                                                                         <  1:277241/137‑1 (MQ=255)
                                                              gTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCTCCGCACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTg                                                                   <  7:85678/137‑1 (MQ=255)
                                                              gTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTg                                                                   <  1:292708/137‑1 (MQ=255)
                                                              gTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTg                                                                   <  3:329495/137‑1 (MQ=255)
                                                               tGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGc                                                                  >  7:11026/1‑137 (MQ=255)
                                                                gCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGcc                                                                 >  2:78220/1‑137 (MQ=255)
                                                                gCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGcc                                                                 >  2:375210/1‑137 (MQ=255)
                                                                  tagcGGGCGTACGCTACCTGGCCATCTCCCAGGCCGCGGCACCCCATGGTTTGTACTAGGCACCCGCCCCTTCCTCGCAATTTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGGCTc                                                               <  6:16211/134‑1 (MQ=255)
                                                                  aaCCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACACCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTc                                                               <  6:266645/137‑1 (MQ=255)
                                                                    ccGGGCGTACGAAACCTGGCAATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGAAAATTGCCTGCTCTATCGGTGGAAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCaa                                                             <  4:23150/137‑1 (MQ=255)
                                                                       ggCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTa                                                          >  2:304786/1‑137 (MQ=255)
                                                                        gCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTAc                                                         <  2:415749/137‑1 (MQ=255)
                                                                         cGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACg                                                        <  5:428896/137‑1 (MQ=255)
                                                                         cGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACg                                                        <  6:80290/137‑1 (MQ=255)
                                                                            aCGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGcc                                                     >  7:155895/1‑137 (MQ=255)
                                                                             cGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCt                                                    <  7:19422/137‑1 (MQ=255)
                                                                                  ccTGGCCATCTCCCAGGCCGCCGCACCCCATTGCATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGGTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCg                                               <  5:420588/137‑1 (MQ=255)
                                                                                     ggCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTGGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTac                                            <  4:337542/137‑1 (MQ=255)
                                                                                     ggCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTac                                            >  7:235744/1‑137 (MQ=255)
                                                                                       ccATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTacac                                          <  8:155895/137‑1 (MQ=255)
                                                                                       ccATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTacac                                          <  8:11026/137‑1 (MQ=255)
                                                                                       ccATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTacac                                          <  7:211646/137‑1 (MQ=255)
                                                                                        cATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTacac                                          >  4:62774/1‑136 (MQ=255)
                                                                                        cATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTacac                                          <  3:62774/136‑1 (MQ=255)
                                                                                              ccAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGc                                   <  2:435856/137‑1 (MQ=255)
                                                                                                cggCCGCCGCACCCCATGGTATGTACTACCCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTc                                 <  2:226660/136‑1 (MQ=255)
                                                                                                     gccgcACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGt                            <  3:310003/137‑1 (MQ=255)
                                                                                                     gccgcACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGt                            <  8:235744/137‑1 (MQ=255)
                                                                                                     cccgcACCCCATGGTATGTACAACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGGGGCAACGTCGCCGAAAACTCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGt                            <  4:121218/136‑1 (MQ=255)
                                                                                                      ccgcACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGAAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTg                           <  1:200892/137‑1 (MQ=255)
                                                                                                        gcACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGa                         <  7:12590/137‑1 (MQ=255)
                                                                                                        gcACCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGa                         <  8:71567/137‑1 (MQ=255)
                                                                                                         cACCCCATGGTATGTACTACGCACACGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCCAGTACGGCCTGACCGTACCCAATGTGCTCAAGGTGGAc                        >  4:178459/1‑137 (MQ=255)
                                                                                                          aCCCCATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGCCCGTACACAATGTGCTCAAGGTGGACa                       >  4:351501/1‑137 (MQ=255)
                                                                                                             ccctGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATcc                    <  2:343655/134‑1 (MQ=255)
                                                                                                             ccATGGTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATcc                    <  8:54838/137‑1 (MQ=255)
                                                                                                                  gTATGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACg               >  8:352378/1‑137 (MQ=255)
                                                                                                                   tATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATcc                    <  1:167531/131‑1 (MQ=255)
                                                                                                                   tATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATcc                    >  2:167531/1‑131 (MQ=255)
                                                                                                                    aTGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGt             <  3:155899/137‑1 (MQ=255)
                                                                                                                    aTGTACTACGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGt             >  7:296628/1‑137 (MQ=255)
                                                                                                                         ctacGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACg                                                        >  8:37603/1‑89 (MQ=255)
                                                                                                                         ctacGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACg                                                        <  7:37603/89‑1 (MQ=255)
                                                                                                                         ctacGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGTCGAgg        <  1:129957/137‑1 (MQ=255)
                                                                                                                         ctacGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGTCGAgg        >  8:36432/1‑137 (MQ=255)
                                                                                                                         ctacGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGTCGAgg        >  2:198130/1‑137 (MQ=255)
                                                                                                                          tacGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGTCGAggg       <  5:91739/137‑1 (MQ=255)
                                                                                                                          aacGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGTTCGGTGGCAACGGCGCCGAAAACGCGGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGTCGAggg       <  6:14839/136‑1 (MQ=255)
                                                                                                                           acGCACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGTCGAGGGc      >  5:17503/1‑137 (MQ=255)
                                                                                                                              cACCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCACCGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGTCGAGGGCGaa   >  4:317679/1‑137 (MQ=255)
                                                                                                                               ccccGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGGGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGTCGAGGGCGAAc  <  5:85205/136‑1 (MQ=255)
                                                                                                                               aCCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGcc                                                                                          <  2:47798/49‑1 (MQ=255)
                                                                                                                               aCCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGcc                                                                                          >  1:47798/1‑49 (MQ=255)
                                                                                                                               aCCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACg                                                        <  7:246190/83‑1 (MQ=255)
                                                                                                                               aCCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACg                                                        >  8:246190/1‑83 (MQ=255)
                                                                                                                               aCCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGt                            >  7:310399/1‑111 (MQ=255)
                                                                                                                               aCCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGt                            <  8:310399/111‑1 (MQ=255)
                                                                                                                               aCCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGTCGAGGGCGCAc  >  3:254061/1‑137 (MQ=255)
                                                                                                                               aCCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGTCGAGGGCGAAc  >  3:273511/1‑137 (MQ=255)
                                                                                                                               aCCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGTCGAGGGCGAAc  <  2:134044/137‑1 (MQ=255)
                                                                                                                               aCCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGTCGAGGGCGAAc  >  3:231352/1‑137 (MQ=255)
                                                                                                                               aCCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGTCGAGGGCGAAc  >  2:67849/1‑137 (MQ=255)
                                                                                                                               aCCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGCCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGTCGAGGGCGAAc  >  1:352452/1‑137 (MQ=255)
                                                                                                                               aCCCGCCCCTTCCTCGAAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGAGTGCACTGCCTCAAGTACGGCCTGACCGTACACAAGGTGCTCAAGGTTGACATCATTACTGTCGAGGGCGacc  >  7:158169/1‑135 (MQ=255)
                                                                                                                                    |                                                                                                                                   
TGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGTCGAGGGCGAAC  >  ACB122/4265965‑4266228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: