| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 10,913 | G→A | 10.0% | W95* (TGG→TAG) | CJ019_00019 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 10,913 | 0 | G | A | 10.0% | 117.3 / 4.2 | 40 | W95* (TGG→TAG) | CJ019_00019 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (19/17); new base A (2/2); total (21/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.62e-01 | |||||||||||
CAGATGATGGAAGGACCACTGAGCTGGCCTGCGGTATCTGCTGAGTTTCGTGATCCAAGGCCCAAGGGGGTCAAGTTATGGACGACTCATCACGCTCGTTACTGTCCGATCTGTTTGGGCGAAGCCGGTTATTGGAGAGAAAGCTGGGGCTTGACCCTGGTTACGACGTGTTCTGTCCACGGCACTGAACTCCACGGGCGGTGCCCCAACTGTCTGAAGGAAACCGATCCAAGCGCAATGAGTGCGAATAGTTGCCAAGCGTGTG > ACB122/10780‑11044 | cAGATGATGGAAGGACCACTGAGCTGGCCTGCGGTATCTGCTGAGTTTCGTGATCCAAGGCCCAAGGGGGTCAAGTTATGGACGACTCATCACGCTCGTTACTGTCCGATCTGTTTGGGCGAAGCCGGTTATTg > 2:72943/1‑134 (MQ=255) gAAGGACCACTGAGCTGGCCTGCGGTATCTGCTGAGTTTCGTGATCCAAGGCCCAAGGGGGTCAAGTTATGGACGACTCATCACGCTCGTTACTGTCCGATCTGTTTGGGCGAAGCCGGTTATTGGAGAGAAAGCt > 7:80824/1‑136 (MQ=255) aGGACCACTGAGCTGGCCTGCGGTATCTGCTGAGTTTCGTGATCCAAGGCCCAAGGGGGTCAAGTTATGGAGGACTCATCACGCTCGTTACTGTCCGATCTGTTTGGGCGAAGCCGGTTATTGGAGAGAAAGCTgg > 2:37347/1‑136 (MQ=255) ggACCACTGCGCGGGCCTGCGGTATCTGCTGAGTTTCGTGATCCAAGGCCCAAGGGGGTCAAGTTATGGACGACTCATCACGCTCGTTACTGTCCGATCTGTTTGGGCGAAGCCGGTTATTGGAGAGAAAGCTggg < 6:170127/136‑1 (MQ=255) gACCACTGAGCTGGCCTGCGGTATCTGCTGAGTTTCGTGATCCAAGGCCCAAGGGGGTCAAGTTATGGACGACTCATCACGCTCGTTACTGTCCGATCTGTTTGGGCGAAGCCGGTTATTGGAGAGAAAGCTggg < 6:153027/135‑1 (MQ=255) gACCACTGAGCTGGCCTGCGGTATCTGCTGAGTTTCGTGATCCAAGGCCCAAGGGGGTCAAGTTATGGACGACTCATCACGCTCGTTACTGTCCGATCTGTTTGGGCGAAGCCGGTTATTGGAGAGAAAGCTggg > 5:153027/1‑135 (MQ=255) cACTGAGCTGGCCTGCGGTATCTGCTGAGTTTCGTGATCCAAGGCCCAAGGGGGTCAAGTTATGGACGACTCATCACGCTCGTTACTGTCCGATCTGTTTGGGCGAAGCCGGTTATTGGAGAGAAAGCTGGGGCtt > 5:35487/1‑136 (MQ=255) cTGAGCTGGCCTGCGGTATCTGCTGAGTTTCGTGATCCAAGGCCCAAGGGGGTCAAGTTATGGACGACTCATCACGCTCGTTACTGTCCGATCTGTTTGGGCGAAGCCGGTTATTGGAGAGAAAGCTGGGGCTTGa < 2:54760/136‑1 (MQ=255) cTGAGCGGGCCTGCGGTAGCTGGGGTGTTTCGTGAGCCACGGCCCCAGGGGGTCAAGTTATGGACGACTCATCACGCTCGTTACTGTCCGATCTGTTTGGGCGAAGCCGGTTATTGGAGAGAAAGCTGGGGCTTGa < 1:105122/136‑1 (MQ=255) gAGCTGGCCTGCGGTATCTGCTGAGTTTCGTGATCCAAGGCCCAAGGGGGTCAAGTTATGGACGACTCATCACGCTCGTTACTGTCCGATCTGTTTGGGCGAAGCCGGTTATTGGAGAGAAAGCTGGGGCTTGAcc > 2:173813/1‑136 (MQ=255) gCCTGCGGTATCTGCTGAGTTTCGTGATCCAAGGCCCAAGGGGGTCAAGTTATGGACGACTCATCACGCTCGTTACTGTCCGATCTGTTTGGGCGAAGCCGGTTATTGGAGAGAAAGCTGGGGCTTGACCCTGGt > 6:176301/1‑135 (MQ=255) ccTGCGGTATCTGCTGAGTTTCGTGATCCAAGGCCCAAGGGGGTCAAGTTATGGACGACTCATCACGCTCGTTACTGTCCGATCTGTTTGGGCGAAGCCGGTTATTGGAGAGAAAGCTGGGGCTTGACCCTGGTTa < 1:173813/136‑1 (MQ=255) cTGCGGTATCTGCTGAGTTTCGTGATCCAAGGCCCAAGGGGGTCAAGTTATGGATGACTCATCACGCTCGTTACTGTCCGATCTGTTTGGGCGAAGCCGGTTATTGGAGAGAAAGCTGGGGCTTGACCCTGGTTac < 1:37347/136‑1 (MQ=255) ctgAGTTTCGTGATCCAAGGCCCAAGGGGGTCAAGTTATGGACGACTCATCACGCTCGTTACTGTCCGATCTGTTTGGGCGAAGCCGGTTATTGGAGAGAAAGCTGGGGCTTGACCCTGGTTACGACGTGTTCTGt < 1:123507/136‑1 (MQ=255) aGTTGCGTGTGCCACGGCCCATGGGGGTCATGTTAGGGACGACTCATCACGCTCGTTACTGTCCGATCTGTTTGGGCGAAGCCGGTTATTGGAGAGAAAGCTGGGGCTTGACCCTGGTTACGACGTGTTCTGTcc < 8:80824/135‑1 (MQ=255) gTTTCGTGATCCAAGGCCCAAGGGGGTCAAGTTATGGACGACTCATCACGCTCGTTACTGTCCGATCTGTTTGGGCGAAGCCGGTTATTGGAGAGAAAGCTGGGGCTTGACCCTGGTTACGACGTGTTCTGTCCAc > 4:80277/1‑136 (MQ=255) gTTTCGTGATCCAAGGCCCAAGGGGGTCAAGTTATGGACGACTCATCACGCTCGTTACTGTCCGATCTGTTTGGGCGAAGCCGGTTATTGGAGAGAAAGCTGGGGCTTGACCCTGGTTACGACGTGTTCTGTCCAc > 6:111497/1‑136 (MQ=255) gTTTCGTGATCCAAGGCCCAAGGGGGTCAAGTTATGGACGACTCATCACGCTCGTTACTGTCCGATCTGTTTGGGCGAAGCCGGTTATTAGAGAGAAAGCTGGGGCTTGACCCTGGTTACGACGTGTTCTGTCCAc > 7:196996/1‑136 (MQ=255) gTTTCGTGATCCAAGGCCCAAGGGGGTCAAGTTATGGACGACTCATCACGCTCGTTACTGTCCGATCTGTTTGGGCGAAGCCGGTTATTAGAGAGAAAGCTGGGGCTTGACCCTGGTTACGACGTGTTCTGTCCAc > 7:193830/1‑136 (MQ=255) cGTGCTCCAAGGCCCACGGGGGGCAAGTTATGGACGACTCATCACGCTCGTTACTGTCCGATCTGTTTGGGCGAAGCCGATTATTGGAGAGAAAGCTGGGGCTTGACCCTGGTTACGACGTGTTCTGTCCACGGCa < 4:125249/136‑1 (MQ=255) tGATCCAAGGCCCAAGGGGGTCAAGTTATGGACGACTCATCACGCTCGTTACTGTCCGATCTGTTTGGGCGAAGCCGGTTATTAGAGAGAAAGCTGGGGCTTGACCCTGGTTACGACGTGTTCTGTCCACGGCACt < 8:193830/136‑1 (MQ=255) tGATCCAAGGCCCAAGGGGGTCAAGTTATGGACGACTCATCACGCTCGTTACTGTCCGATCTGTTTGGGCGAAGCCGGTTATTAGAGAGAAAGCTGGGGCTTGACCCTGGTTACGACGTGTTCTGTCCACGGCACt < 8:196996/136‑1 (MQ=255) ggCCCAAGGGGGTCAAGTTATGGACGACTCATCACGCTCGTTACTGTCCGATCTGTTTGGGCGAAGCCGGTTATTGGAGAGAAAGCTGGGGCTTGACCCTGGTTACGACGTGTTCTGTCCACGGCCCTGAACTCCa > 3:153841/1‑136 (MQ=255) cAAGGGGGTCAAGTTATGGACGACTCATCACGCTCGTTACTGTCCGATCTGTTTGGGCGAAGCCGGTTATTGGAGAGAAAGCTGGGGCTTGACCCTGGTTACGACGTGTTCTGTCCACGGCACTGAACTCCACggg < 8:150493/136‑1 (MQ=255) aaGTTATGGACGACTCATCACGCTCGTTACTGTCCGATCTGTTTGGGCGAAGCCGGTTATTGGAGAGAAAGCTGGGGCTTGACCCTGGTTACGACGTGTTCTGTCCACGGCACTGAACTCCACGGGCGGTGcccc > 4:71562/1‑135 (MQ=255) catcaCGCTCGTTACTGTCCGATCTGTTTGGGCGAAGCCGGTTATTGGAGAGAAAGCTGGGGCTTGACCCTGGTTACGACGTGTTCTGTCCCCGGCACTGAACTCCACGGGCGGTGCCCCAACTGTCTGAAGGaaa > 3:9083/1‑136 (MQ=255) atcaCGCTCGTTACTGTCCGATCTGTTTGGGCGAAGCCGGTTATTGGAGAGAAAg > 8:191608/1‑55 (MQ=255) atcaCGCTCGTTACTGTCCGATCTGTTTGGGCGAAGCCGGTTATTGGAGAGAAAg < 7:191608/55‑1 (MQ=255) atcaCGCTCGTTACTGTCCGATCTGTTTGGGCGAAGCCGGTTATTGGAGAGAAAGCTGGGGCTTGACCCTGGTTACGACGTGTTCTGTCCACGGCACTGAACTCCACGGGCGGTGCCCCAACTGTCTGAAGGAAAc > 8:22206/1‑136 (MQ=255) cTCGTTACTGTCCGATCTGTTTGGGCGAAGCCGGTTATTGGAGAGAAAGCTGGGGCTTGACCCTGGTTACGACGTGTTCTGTCCACGGCACTGAACTCCACGGGCGGTGCCCCAACTGTCTGAAGGAAACCGATcc < 3:80277/136‑1 (MQ=255) cTCGTTACTGTCCGATCTGTTTGGGCGAAGCCGGTTATTGGAGAGAAAGCTGGGGCTTGACCCTGGTTACGACGTGTTCTGGCCACGGCACTGAACTCCACGGGCGGTGCCCCAACTGTCTGAAGGAAACCGATcc < 5:176301/136‑1 (MQ=255) gATCTGTTTTGGCGAAGCCGGTTATTGGAGAGAAAGCTGGGGCTTGACCCTGGTTACGACGTGTTCTGTCCACGGCACTGAACTCCACGGGCGGTTCCCCAACAGTCTGAAGGAAACCCATCCAAGCGCAATGAGt > 4:204037/1‑136 (MQ=255) gATCTGTTTGGGCGAAGCCGGTTATTGGAGAGAAAGCTGGGGCTTGACCCTGGTTACGACGTGTTCTGTCCACGGCACTGAACTCCACGGGCGGTGCCCCAACTGTCTGAAGGAAACCGATCCAAGCGCAATGAGt > 2:103167/1‑136 (MQ=255) cTGTTTGGGCGAAGCCGGTTATTGGAGAGAAAGCTGGGGCTTGACCCTGGTTacga > 4:129632/1‑56 (MQ=255) cTGTTTGGGCGAAGCCGGTTATTGGAGAGAAAGCTGGGGCTTGACCCTGGTTacga < 3:129632/56‑1 (MQ=255) gTTTGGGCGAAGCCGGTTATTGGAGAGAAAGCTGGGGCTTGACCCTGGTTACGACGTGTTCTGTCCACGGCACTGAACTCCACGGGCGGTGCCCCAACTGTCTGAAGGAAACCGATCCAAGCGCAATGAGTGCGaa > 5:28966/1‑136 (MQ=255) gTTTGGGCGAAGCCGGTTATTGGAGAGAAAGCTGGGGCTTGACCCTGGTTACGACGTGTTCTGTCCACGGCACTGAACTCCACGGGCGGTGCCCCAACTGTCTGAAGGAAACCGATCCAAGCGCAATGAGTGCGaa > 4:84501/1‑136 (MQ=255) cgtcgCCTGTTATTGGAGAGAAAGCTGGGGCGTGACCCTGGTTACGACGTGTTCTGTCCACGGCACTGAACTCCACGGGCGGTTCCCCAACTGTCTGAAGGAAACCGATCCAAGCGCAATGAGTGCGAATAGTTGc < 7:110515/132‑1 (MQ=255) cGAAGCCGGTTATTGGAGAGAAAGCTGGGGCTTGACCCTGGTTACGACGGTGTCTGTCCACGGCACTGAACTCCACGGGCGGTGCCCCAACTGTCTGAAGGAAACCGATCCAAGCGCAATGAGTGCGAATAGTTGc < 1:102930/136‑1 (MQ=255) ggTTATTGGAGAGAAAGCTGGGGCTTGACCCTGGTTACGACGTGTTCTGTCCACGGCACTGAACTCCACGGGCGGTGCCCCAACTGTCTGAAGGAAACCGATCCAAGCGCAATGAGTGCGAATAGTTGCCAAGCgt > 8:205943/1‑136 (MQ=255) aTTGGAGAGAAAGCTGGGGCTTGACCCTGGTTACGACGTGTTCTGTCCACGGCACTGAACTCCACGGGCGGTGCCCCAACTGTCTGAAGGAAACCGATCCAAGCGCAATGAGTGCGAATAGTTGCCAAGCGtgtg < 1:72943/135‑1 (MQ=255) | CAGATGATGGAAGGACCACTGAGCTGGCCTGCGGTATCTGCTGAGTTTCGTGATCCAAGGCCCAAGGGGGTCAAGTTATGGACGACTCATCACGCTCGTTACTGTCCGATCTGTTTGGGCGAAGCCGGTTATTGGAGAGAAAGCTGGGGCTTGACCCTGGTTACGACGTGTTCTGTCCACGGCACTGAACTCCACGGGCGGTGCCCCAACTGTCTGAAGGAAACCGATCCAAGCGCAATGAGTGCGAATAGTTGCCAAGCGTGTG > ACB122/10780‑11044 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |