Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 956,989 C→T 13.8% P183L (CCG→CTG)  metN_3 → Methionine import ATP‑binding protein MetN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB122956,9890CT13.8% 75.3 / 4.8 29P183L (CCG→CTG) metN_3Methionine import ATP‑binding protein MetN
Reads supporting (aligned to +/- strand):  ref base C (17/8);  new base T (2/2);  total (19/10)
Fisher's exact test for biased strand distribution p-value = 5.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.32e-01

TGGGCCTGGCCGACAAGCTGTTCGTGCAGTGTGGGCAGTTGTCCGGTGGCCAGTTGCAGCGGGTGGGCATTGCTCGGGCGTTGTACCAGCGTCCGCAGGTGCTGCTGACCGACGAACCGGTGTCGGCCATGGACCCGGTGTTGGCCGACCATAGCCTGGCCTTGCTCAATCGCCATGCGCAGGCCACTGGCGTGACGTTGGTGGCGAGCCTGCACGCCGTAGAGTTGGCGCTGGCGCATTTTCCGCGGGTGATTGGCATTCGGGAAGGGC  >  ACB122/956854‑957123
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tGGGCCTGGCCGACAAGCTGTTCGTGCAGTGTGCGCAGTTCTCCGGTGGCCAGTTGCCGCGGGTGGGCAATGCTCGGGCGTTGTACCAGCGTCCGCAGGTGCTGCTGACCGACGAACCGGTGTCGGCCATGGAccc                                                                                                                                        >  3:191122/1‑136 (MQ=255)
           gACAAGCTGTTCGTGCAGTGTGGGCAGTTGTCCGGTGGCCAGTTGCAGCGGGTGGGCATTGCTCGGGCGGTGTACCAGCGTCCGCAGGTGCTGCTGACCGACGAACCGGTGTCGGCCATGGACCCGGTGTTGGCCg                                                                                                                             <  8:181669/136‑1 (MQ=255)
                 cTGTTCGTGCAGTGTGGGCAGTTGTCCGGTGGCCAGTTGCAGCGGGTGGGCATTGCGCGGGCGTTGTACCAGCGTCCGCAGGTGCTGCTGACCGACGAACCGGTGTCGGCCATGGACCCGGTGTTGGCCGACCATa                                                                                                                       <  7:30944/136‑1 (MQ=255)
                                 ggCAGTTGTCCGGTGGCCAGTTGCAGCGGGTGGGCATTGCTCGGGCGTTGTACCAGCGTCCGCAGGTGCTGCTGACCGACGAACCGGTGTCGGCCATGGACCCGGTGTTGGCCGACCATAGCCTGGCCTTGCTCaa                                                                                                       >  5:178690/1‑136 (MQ=255)
                                   cAGTTGTCCGGTGGCCAGTTGCAGCGGGTGGGCATTGCTCGGGCGTTGTACCAGCGTCCGCAGGTGCTGCTGACCGACGAACCGGTGTCGGCCATGGACCCGGTGTTGGCCGACCATAGCCTGGCCTTGCTCAATc                                                                                                     <  4:195612/136‑1 (MQ=255)
                                          ccGGTGGCCAGTTGCAGCGGGTGGGCATTGCTCGGGCGTTGTACCAGCGTCCGCAGGTGCTGCTGACCGACGAACCGGTGTCGGCCATGGACCCGGTGTTGGCCGACCATAGCCTGGCCTTGCTCAATCGCCATgc                                                                                              >  3:5433/1‑136 (MQ=255)
                                                   agatGCAGCGGGTGGGCATTGCTCGCGCCTTGTACCAGCGTCACCAGGTCCTCCTGACAGACGAACCGGTGTCGGCCATGGACCCGCTGTTGGAAGCCCATAGCCTGTCCTTGCTCAATCGCCATGATCAGGCCa                                                                                      >  8:123080/4‑135 (MQ=255)
                                                      tGCAGCGGGTGGGCATTGCTCGGGCGTTGTACCAGCGTCCGCAGGTGCTGCTGACCGACGAACCGGTGTCGGCCATGGACCCGGTGTTGGCCGACCATAGCCTGGCCTTGCTCAATCGCCATGCGCAGGCCACTgg                                                                                  >  6:186117/1‑136 (MQ=255)
                                                        cAGCGGGTGGGCATTGCTCGGGCGTTGTACCAGCGTCCGCAGGTGCTGCTGACCGACGAACCGGTGTCGGCCATGGACCCGGTGTTGGCCGACCATAGCCTGGCCTTGCTCAATCGCCATGCGCAGGCCACTGGCg                                                                                >  5:203496/1‑136 (MQ=255)
                                                                gggCATTGCTCGGGCGTTGTACCAGCGTCCGCAGGTGCTGCTGACCGACGAACCGGTGTCGGCCATGGACCCGGTGTTGGCCGACCATAGCCTGGCCTTGCTCAATCGCCATGCGCAGGCCACTGGCGTGACGTtg                                                                        >  7:15680/1‑136 (MQ=255)
                                                                          cGGGCGTTGTACCAGCGTCCGCAGGTGCTGCTGACCGACGAACCGGTGTCGGCCATGGACCCGGTGTTGGCCGACCATAGCCTGGCCTTGCTCAATCGCCATGCGCAGGCCACTGGCGTGACGTTGGTGGCGAGcc                                                              >  4:129273/1‑136 (MQ=255)
                                                                           gtgCGGTGTACCAGCTTCCGCAGGTGCTGCTGACCGACGAACCTGTGTCGGCCATGGACCCGTTGTTGGCAGACCATAGCCTGGCCTTGCTCACTCGCCATGAGCAGGCCACTGGCGTGACGTTGGTGGCGAGCCt                                                             <  6:5338/134‑1 (MQ=255)
                                                                           gggCGTTGTACCAGCGTCCGCAGGTGCTGCTGACCGACGAACCGGTGTCGGCCATGGACCCGGTGTTGGCCGACCATAGCCTGGCCTTGCTCAATCGCCATGCGCAGGCCACTGGCGTGACGTTGGTGGCGAGCCt                                                             >  2:34968/1‑136 (MQ=255)
                                                                           gggCGTTGTACCAGCGTCCGCAGGTGCTGCTGACCGACGAACCGGTGTCGGCCATGGACCCGGTGTTGGCCGACCATAGCCTGGCCTTGCTCAATCGCCATGCGCAGGCCACTGGCGTGACGTTGGTGGCGAGCCt                                                             <  3:129273/136‑1 (MQ=255)
                                                                                     ccAGCGTCCGCAGGTGCTGCTGACCGACGCACCGGTGTCGGCCATGGACCCGGTGTTGGCCGACCATAGCCTGGCCTTGCTCAATCGCCATGCGCAGGCCACTGGCGTGACGTTGGTGGCGAGCCTGCACGCCGTa                                                   >  3:203152/1‑136 (MQ=255)
                                                                                     ccAGCGTCCGCAGGTGCTGCTGACCGACGAACCGGTGTCGGCCATGGACCCGGTGTTGGCCGACCATAGCCTGGCCTTGCTCAATCGCCATGCGCAGGCCACTGGCGTGACGTTGGTGGCGAGCCTGCACGCCg                                                     >  8:92082/1‑134 (MQ=255)
                                                                                          gTCCGCAGGTGCTGCTGACCGACGAACCGGTGTCGGCCATGGACCCGGTGTTGGCCGACCATAGCCTGGCCTTGCTCAATc                                                                                                     <  8:88350/81‑1 (MQ=255)
                                                                                          gTCCGCAGGTGCTGCTGACCGACGAACCGGTGTCGGCCATGGACCCGGTGTTGGCCGACCATAGCCTGGCCTTGCTCAATc                                                                                                     >  7:88350/1‑81 (MQ=255)
                                                                                          gTCCGCAGGTGCTGCTGACCGACGAACCGGTGTCGGCCATGGACCCGGTGTTGGCCGACCATAGCCTGGCCTTGCTCAATCGCCATGCGCAGGCCACTGGCGTGACGTTGGTGGCGAGCCTGCACGCCGTAGAGtt                                              >  7:135612/1‑136 (MQ=255)
                                                                                              gCAGGTGCTGCTGACCGACGAACCGGTGTCGGCCATGGACCCGGTGTTGGCCGACCATAGCCTGGCCTTGCTCAATCGCCATGCGCAGGCCACTGGCGTGACGTTGGTGGCGAGCCTGCACGCCGTAGAGTtggcg                                          >  3:65738/1‑136 (MQ=255)
                                                                                                aGGTGCTGCTGACCGACGAACCGGTGTCGGCCATGGACCTGGTGTTGGCCGACCATAGCCTGGCCTTGCTCAATCGCCATGCGCAGGCCACTGGCg                                                                                <  1:93415/96‑1 (MQ=255)
                                                                                                aGGTGCTGCTGACCGACGAACCGGTGTCGGCCATGGACCTGGTGTTGGCCGACCATAGCCTGGCCTTGCTCAATCGCCATGCGCAGGCCACTGGCg                                                                                <  1:93416/96‑1 (MQ=255)
                                                                                                aGGTGCTGCTGACCGACGAACCGGTGTCGGCCATGGACCTGGTGTTGGCCGACCATAGCCTGGCCTTGCTCAATCGCCATGCGCAGGCCACTGGCg                                                                                >  2:93415/1‑96 (MQ=255)
                                                                                                aGGTGCTGCTGACCGACGAACCGGTGTCGGCCATGGACCTGGTGTTGGCCGACCATAGCCTGGCCTTGCTCAATCGCCATGCGCAGGCCACTGGCg                                                                                >  2:93416/1‑96 (MQ=255)
                                                                                                aGGTGCTGCTGACCGACGAACCGGTGTCGGCCATGGACCCGGTGTTGGCCGCCCATAGCCTGGCCTTGCTCAATCGCCATGCGCAGGCCACTGGCGTGACGTTGGTGGCGAGCCTGCACGCCGTAGAGTtggcgct                                        >  7:141700/1‑136 (MQ=255)
                                                                                                aGGTGCTGCTGACCGACGAACCGGTGTCGGCCATGGACCCGGTGTTGGCCGACCATAGCCTGGCCTTGCTCAATCGCCATGCGCAGGCCCCTGGCGTGCCGTTGGTGGCGAGCCTGCACGCCGGAGAGTtggcgcg                                        >  5:120859/1‑135 (MQ=255)
                                                                                                aGGTGCTGCTGACCGACGAACCGGTGTCGGCCATGGACCCGGTGTTGGCCGACCATAGCCTGGCCTTGCTCAATCGCCATGCGCAGGCCACTGGCGTGACGTTGGTGGCGAGCCTGCACGCCGTAGAGTtggcgct                                        >  8:19149/1‑136 (MQ=255)
                                                                                                           aCCGACGAACCGGTGTCGGCCATGGACCCGGTGTTGGCCGACCATAGCCTGGCCTTGCTCAATCGCCATGCGCAGGCCACTGGCGTGACGTTGGTGGCGAGCCTGCACg                                                        <  6:21691/109‑1 (MQ=255)
                                                                                                           aCCGACGAACCGGTGTCGGCCATGGACCCGGTGTTGGCCGACCATAGCCTGGCCTTGCTCAATCGCCATGCGCAGGCCACTGGCGTGACGTTGGTGGCGAGCCTGCACg                                                        >  5:21691/1‑109 (MQ=255)
                                                                                                            ccGACGAAGCGGTGTCGGCCATGGACCCGGTGTTGGCCGAGCATAGCCTGGCCTTGCTCAATCGCCATGCGCAGGCCACTGGCGTGACGTTGGTGGCGAGCCTGCACGCCGTAGAGTTGGCGCTGGCGCATTTTcc                            <  4:203152/136‑1 (MQ=255)
                                                                                                                                     cccGGTGTTGGCCGACCATAGCCTGGCCTTGCTCAATCGCCATGCGCAGGCCACTGGCGTGACGTTGGTGGCGAGCCTGCACGCCGTAGAGTTGGCGCTGGCGCATTTTCCGCGGGTGATTGGCATTCGGGaa      >  6:77078/1‑133 (MQ=255)
                                                                                                                                     cccGGTGTTGGCCGACCATAGCCTGGCCTTGCTCAATCGCCATGCGCAGGCCACTGGCGTGACGTTGGTGGCGAGCCTGCACGCCGTAGAGTTGGCGCTGGCGCATTTTCCGCGGGTGATTGGCATTCGGGaa      <  5:77078/133‑1 (MQ=255)
                                                                                                                                      ccGGTGTTGGCCGACCATAGCCTGGCCTTGCTCAATCGCCATGCGCAGGCCACTGGCGTGACGTTGGTGGCGAGCCTGCACGCCGTAGAGTTGGCGCTGGCGCATTTTCCGCGGGTGATTGGCATTCGGGAAGGGc  <  4:5433/136‑1 (MQ=255)
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TGGGCCTGGCCGACAAGCTGTTCGTGCAGTGTGGGCAGTTGTCCGGTGGCCAGTTGCAGCGGGTGGGCATTGCTCGGGCGTTGTACCAGCGTCCGCAGGTGCTGCTGACCGACGAACCGGTGTCGGCCATGGACCCGGTGTTGGCCGACCATAGCCTGGCCTTGCTCAATCGCCATGCGCAGGCCACTGGCGTGACGTTGGTGGCGAGCCTGCACGCCGTAGAGTTGGCGCTGGCGCATTTTCCGCGGGTGATTGGCATTCGGGAAGGGC  >  ACB122/956854‑957123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑