| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 1,052,536 | T→G | 30.8% | S90R (AGC→CGC) | argO ← | Arginine exporter protein ArgO |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 1,052,536 | 0 | T | G | 30.8% | 24.5 / 3.0 | 33 | S90R (AGC→CGC) | argO | Arginine exporter protein ArgO |
| Reads supporting (aligned to +/- strand): ref base T (18/4); new base G (6/5); total (24/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGCAACACGGTGTCGAGGTACACGTGCGGGTTCAGCAGGGTCACCGCCAGGGCGCTGAGCAGTACGGCGCGACGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCAGCGCCTTGGCGCCGTACCAGATCAGGAACACTGCGCCGCCCCAGCGGGCAATCGCCAGCAAGGTCGGGTTGTGTGCCAGCACGGTGGCCAGTCCGAATACTCCGGCGGCCACGAGGATGGCGTCA > ACB122/1052408‑1052666 | cAGCAACACGGTGTCGAGGTACACGTGCGGGTTCAGCAGGGTCACCGCCAGGGCGCTGAGCAGTACGGCGCGACGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGcgccgc > 6:23117/1‑136 (MQ=255) gCAACACGGTGTCGAGGTACACGTGCGGGTTCAGCAGGGTCACCGCCAGGGCGCTGAGCAGTACGGCGCGACGTGAGCGAACGCCTTGGTCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCGGCGCCgcgc > 2:18318/1‑136 (MQ=255) cGAGGTACACGTGCGGGTTCAGCAGGGTCACCGCCAGGGCGCTGAGCAGTACGGCGCGACGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCAGCGCCATGGCGCCGTa > 2:199766/1‑136 (MQ=255) gTTCAGCAGGGTCACCGCCAGGGCGCTGAGCAGTACGGCGCGACGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCAGCGCCTTGGCGCCGTACCAGATCAGGAACACt > 6:32250/1‑136 (MQ=255) agGGTCACCGCCAGGGCGCTGAGCAGTACGGCGCGACGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCGGCGCCGCGCCATCGCGCCGTACCAGATCAGGAACACTAcgccgc > 6:203516/1‑136 (MQ=255) ccAGGGCGCTGAGCAGTACGGCGCGACGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCAGCGCCTTGGCGCCGTACCAGAt > 8:192201/1‑109 (MQ=255) ccAGGGCGCTGAGCAGTACGGCGCGACGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCAGCGCCTTGGCGCCGTACCAGAt < 7:192201/109‑1 (MQ=255) aGGGCGCTGAGCAGTACGGCGCGACGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCAGCGCCTTGGCGGCGGCCCCGCTCAGGAACACTGCGCCGCCCCAGCGGGcga > 7:14192/1‑134 (MQ=255) cgcTTAGCTGTCCGGCGCGACGGGAGCGACCGCCTTGGCGTGGCTGGGGAGGCCGGGGGTGCGTGCCGCGGGCGGGGCGCTGCGCCTTGGCGCCGTACCAGATCAGGAACACTGCGCCGCCCCAGCGGGCAATCGc < 5:167590/136‑1 (MQ=255) gcTGAGCAGTACGGCGCGACGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCCGCGCCTTGGCGCCGGCCCAGATCAGGATCACTGCGCCGCCCCCCCGGGCAATCGcc > 5:69783/1‑136 (MQ=255) cGGCGCGACGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCAGCGCCTTGGCGCCGTACCAGATCAGGAACACTGCGCCGCCCCAGCGGGCAATCGCCAGCAAGGTCgg < 3:52849/136‑1 (MQ=255) ggCGCGACGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCCGCGCCTTGGCGCCGTACCAGATCAGGAACACTGCGCCGCCCCCGCGGGCAATCGCCAGCAAGGTCggg > 4:124741/1‑136 (MQ=255) gACGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCCGCGCCTTGGCGCCGTACCAGATCAGGAACCCTGCGCCGCCCCCGCCGGCAATCGCCAGCAAGGGCGGGTtgtg > 2:191561/1‑136 (MQ=255) gACGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCCGCGCCTTGGCGCCGGACCAGATCAGGAACACAGCGCCGCCCCAGCGGGCCAGCGCCCGCAAGGTCGGGTtgtg > 7:56935/1‑136 (MQ=255) gACGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCAGCGCCTTGGCGCCGGACCAGATCAGGAACACAGCGCCGCCCCCGCGGGCAAGCGCCCGCACGGGCGGGTtgtg > 4:81058/1‑136 (MQ=255) gACGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCGGCGCCGCGCCCTGGGGCCCTCCCCGCTCAGGCACACTGCGCCGCCCCCCCCCGCCAGAGCCAGCAAGGGCggggggtg > 3:33620/1‑131 (MQ=255) cGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCAGCGCCTTGGCGCCGTACCAGATCAGGAACACTGCGCCGCCCCAGCGGGCCATCGCCCGCAAGGTCGGGTtgtgtg > 7:197331/1‑136 (MQ=255) gAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGGGCGGCGCCGCGCCGTGGCGCCGTCCCCGATCAGGACCACTGCGCCGCCCCCCCGGGCCAGCGCCCGCCACGGCGGGGggtgtga > 8:4391/1‑133 (MQ=255) gAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCCGCGCCGTGGCGCCGGACCCGCTCAGGAACACTGCGCCGCCCCCGCGGGCCCTCGCCCGCCAGGTCGGGTTGTGTGc > 1:79316/1‑134 (MQ=255) gAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCGGCGCCGCGCCTTGGCGCCGGCCCAGATCAGGATCACTGCGCCGCCCCCGCCGGCCCACGCCCGCCACGTCGGGTTGTGTGccc > 4:193730/1‑135 (MQ=255) gAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCGGCGCAGCGCCTTGGCGCCGGACCAGATCAGGAACCCTGCGCCCCCCCACCGGGCACTCGCCCGCCAGGTCGGGGTGTGTGGCa > 3:16025/1‑136 (MQ=255) cgggtgggcattgCTGCTGCGGAAGGCGTGGGTGGGAGCTCGCGCTGCGCAGCGCGTTGGAGCGGTACCAGATCAGGAACCCTGCGCCGCCCCAGCGTTCAATCGCGAGCTAGGTCGGGTTGTGTGCCAGCACGGt < 2:108065/130‑1 (MQ=255) gccttgggcttgCTGGGGCTGCAGGCTCTGCTTGGAGCAGGCGCGGCGCAGCGCCTTGGCGCCGTACCAGATCAGGAACACTGCGCCGCCCCAGCGGGCAATCGCCAGCAAGGTCGGGTTGTGTGCCAGCACGGTg < 1:18318/136‑1 (MQ=255) gccttggccttgCTGGTGCTGCAGGCGCTGCTTGGAGCAGGCGCGGCGCAGCGCCTTGGCGCCGTACCAGATCAGGAACACTGCGCCGCCCCAGCGGGCAATCGCCAGCAAGGTCGGGTTGTGTGCCAGCACGGTg < 1:149684/136‑1 (MQ=255) cttggccttgCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCCGCGCCTTGGCGCCGTACCAGATCAGGAACACTGCGCCGCCCCAGCGGGCAATCGCCAGCAAGGTCGGGTTGTGTGCCAGCACGGTGGc > 6:96128/1‑136 (MQ=255) ggTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCAGCGCCTTGGCGCCGTACCAGATCAGGACCACTGCGCCGCCCCAGCGGGCAATCGCCAGCAAGGTCGGGTTGTGTGCCAGCCCGGTGGCCAGTCCGAATa > 2:77046/1‑135 (MQ=255) ggTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCAGCGCCTTGGCGCCGTACCAGATCAGGAACACTGCGCCGCCCCAGCGGGCAATCGCCAGCAAGGTCGGGTTGTGTGCCAGCACGGTGGCCAGTCCGAATa > 6:151101/1‑135 (MQ=255) ggcggcAGGCTCGGCTGGGCGCAGGCGCGGCGCAGCGCCTTGGCGCCGTACCAGATCAGGAACACTGCGCCGCCCCAGCGGGCAATCGCCAGCAAGGTCGGGTTGTGTGCCAGCACGGTGGCCAGTCCGAATACTc < 7:186443/132‑1 (MQ=255) gCTCTGCTTGGAGCAGGCGCTGCGCCGCGCCTTGGCGCCGTTCCAGATCAGGAACACTGCGCCCCCCCCGCGGGCCATCCCCCGCAAGGTCGGGTTGTGTGCCCGCCCGGTGGCCCTGCCGAATACACCGGCGGcc > 2:203190/1‑136 (MQ=255) gCTTGGAGCAGGCGCTGCGCAGCGCCTTGGGGCCGTACCAGATCAGGAACACTGCGCCGCCCCAGCGGGCAATCGCCAGCAAGGTCCGGTTGTGTGGCAGCCCGGTGGCCAGTCCGAATACACCGGCGGCCCCgcg > 2:78886/1‑134 (MQ=255) ccggCGCTGCGCAGCGCCTTGGCGCCGTACCAGATCAGGAACACTGCGCCGCCCCAGCGGGCAATCGCCAGCAAGGTCGGGTTGTGTGCCAGCACGGTGGCCAGTCCGAATACTCCGGCGGCCACGAGGATGGCGt < 5:79890/134‑1 (MQ=255) cAGGCGCGGCGCAGCGCCTTGGCGCCGTACCAGATCAGGAACACTGCGCCGCCCCAGCGGGCAATCGCCAGCAAGGTCGGGTTGTGTGCCAGCACGGTGGCCAGTCCGAATACTCCGGCGGCCACGAGGATGGCGt < 4:33620/136‑1 (MQ=255) ggCGCTGCGCAGCGCCATGGCGCCGTACCAGATCAGGAACACAGCGCCGCCCCACCGGGCACTCGCCACCAAGGTCGGGTTGTGTGCCAGCACGGTGGCCCGTCCGAATACTCCGGCCGCCCCGAGGATGGCGtga > 3:55376/1‑134 (MQ=255) | CAGCAACACGGTGTCGAGGTACACGTGCGGGTTCAGCAGGGTCACCGCCAGGGCGCTGAGCAGTACGGCGCGACGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCAGCGCCTTGGCGCCGTACCAGATCAGGAACACTGCGCCGCCCCAGCGGGCAATCGCCAGCAAGGTCGGGTTGTGTGCCAGCACGGTGGCCAGTCCGAATACTCCGGCGGCCACGAGGATGGCGTCA > ACB122/1052408‑1052666 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |