Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,309,442 C→T 10.2% intergenic (+12/‑194) CJ019_01229 → / → aaeB_2 hypothetical protein/p‑hydroxybenzoic acid efflux pump subunit AaeB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,309,4420CT10.2% 110.6 / 4.1 39intergenic (+12/‑194)CJ019_01229/aaeB_2hypothetical protein/p‑hydroxybenzoic acid efflux pump subunit AaeB
Reads supporting (aligned to +/- strand):  ref base C (14/21);  new base T (2/2);  total (16/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.23e-01

GTTCTTCGACAACGTGGTTATCGCCGGTGTGGTAACGGTCGGGCTGATGGTCGCCTTCTTTGCCGGTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCTGAGCTGTCCCTTTCAGTGCAGGCCAATCGAATATACATGATTAGCTAGCTAATTAATCG  >  ACB122/1309316‑1309568
                                                                                                                              |                                                                                                                              
gTTCTTCGACAACGTGGTTATCGCCGGTGTGGTAACGGTCGGGCTGATGGTCGCCTTCTTTGCCGGTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCAc                                                                                                                        <  1:111767/135‑1 (MQ=255)
  tcttctACAACGTGGTTATCGCCGGGGTGGGAAGGGTCGGGCTGATGGTCGCCTTCTTTGCCGGTATGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGTGTACACGAGCACg                                                                                                                       <  4:141107/134‑1 (MQ=255)
  tcttcGACAACGTGGTTATCGCCGGTGTGGTAACGGTCGGGCTGATGGTCGCCTTCTTTGCCGGTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACg                                                                                                                       <  5:163929/134‑1 (MQ=255)
               ggTTATCGCCGGTGTGGTAACGGTCGGGCTGATGGTCGCCTTCTTTGCCGGTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAggg                                                                                                         >  3:121521/1‑135 (MQ=255)
                       ccGGTGTGGTAACGGTCGGGCTGATGGTCGCCTTCTTTGCCGGTCTGGGCATTTTCATCTGGAAGGCCTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCg                                                                                                 >  1:337966/1‑135 (MQ=255)
                                 aaCGGGCGGGCTGATGGTCGCCTTCTTTGCCGGTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCAtt                                                                                       <  5:357679/135‑1 (MQ=255)
                                        gggCTGATGGTCGCCTTCTTTGCCGGTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTg                                                                                >  4:171699/1‑135 (MQ=255)
                                        gggCTGATGGTCGCCTTCTTTGCCGGTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTg                                                                                <  3:288999/135‑1 (MQ=255)
                                        gggCTGATGGTCGCCTTCTTTGCCGGTCTGGGCAATTTAATCTGGAAGGCCTCGAACAACCGCAAGCCCCCCTGACCATGCCGGTACACGAGCACCCAAGGCAATTAGGGCGACGTCGGGCGCCCCAATTTTTTg                                                                                >  2:193475/1‑135 (MQ=255)
                                          gCTGATGGTCGCCTTCTTTGCCGGTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGcc                                                                              <  2:323294/135‑1 (MQ=255)
                                           cTGATGGTCGCCTTCTTTGCCGGTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCt                                                                             >  7:311279/1‑135 (MQ=255)
                                            tGATGGTCGCCTTCTTTGCCGGTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCt                                                                             >  5:55938/1‑134 (MQ=255)
                                             gATGGTCGCCTTCTTTGCCGGTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCGCATTTTTTTTGCCTGc                                                                           >  1:219011/1‑135 (MQ=255)
                                                  tCGCCTTCTTTGCCGGTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCAttt                                                                       <  8:244317/134‑1 (MQ=255)
                                                  ccGCCTTCTTTGCCGGTCTGGGCATTTTCATCTGGAAGGACTCGAACAACCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCAttt                                                                       <  5:292651/133‑1 (MQ=255)
                                                   cGCCTTCTTTGCCGGTCTGGGCATTTTAATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCAGTTTTTTTTCCTGCAttttt                                                                     >  3:224394/1‑135 (MQ=255)
                                                      cttcttTGCCGGTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTAt                                                                  <  8:78602/135‑1 (MQ=255)
                                                          tttGCCGGTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTg                                                                <  8:351750/133‑1 (MQ=255)
                                                           ttGCCGGTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTc                                                              >  4:356335/1‑134 (MQ=255)
                                                             gCCGGTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCt                                                             <  7:192216/133‑1 (MQ=255)
                                                             gCCGGTCTGCGCATTTTACTATGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCt                                                             <  7:150365/133‑1 (MQ=255)
                                                              ccGGTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCTGa                                                           <  3:356335/134‑1 (MQ=255)
                                                                ggTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCTGAGCt                                                        <  7:232318/135‑1 (MQ=255)
                                                                ggTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCTGAGCt                                                        <  6:55938/135‑1 (MQ=255)
                                                                     gggCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCTGAGCTGTccc                                                   <  8:374925/135‑1 (MQ=255)
                                                                         aTTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCAATTTTTTTGCGTGAATTTTTTATTATCTGAGGTGTCGCtt                                                 >  6:287707/1‑133 (MQ=255)
                                                                              cATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTATACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCt                                                             <  3:290841/116‑1 (MQ=255)
                                                                              cATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTATACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCt                                                             <  3:285737/116‑1 (MQ=255)
                                                                              cATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTATACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCt                                                             >  4:290841/1‑116 (MQ=255)
                                                                              cATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTATACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCt                                                             >  4:285737/1‑116 (MQ=255)
                                                                                  tGGAAGGACTCGAACAAGCCCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAATGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCTGAGCTGTCCCTTTCAGTGCAGGc                                      <  6:60599/135‑1 (MQ=255)
                                                                                   ggAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCTGAGCTGTCCCTTTCAGTGCAGGc                                      >  4:96388/1‑134 (MQ=255)
                                                                                       cgACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCTGAGCTGTCCCTTTCAGTGCAGGCCAATc                                 <  3:25721/134‑1 (MQ=255)
                                                                                            cGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCTGAGCTGTCCCTTTCAGTGCAGGCCAATCGAat                             >  8:306448/1‑134 (MQ=255)
                                                                                                        cgCCGCGCTGACCTTGCGGGTACACGAGCACTCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCTGAGCTGTCCCTTTCAGTGCAGGCCAATCGAATATACATGATtagc                <  5:287707/134‑1 (MQ=255)
                                                                                                        aGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCTGAGCTGTCCCTTTCAGTGCAGGCCAATCGAATATACATGATtagc                <  2:34341/135‑1 (MQ=255)
                                                                                                                tGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCTGAGCTGTCCCTTTCAGTGCAGGCCAATCGAATATACATGATTAGCTAGCtaa         >  6:282028/1‑134 (MQ=255)
                                                                                                                 gACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCACTTTTTATTGTCTGAGCTGTCCCTTTCAGTGCAGGCCAATCGAATATACATGATTAGCTAGCtaat        >  1:60130/1‑134 (MQ=255)
                                                                                                                        cGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCTGAGCTGTCCCTTTCAGTGCAGGCCAATCGAATATACATGATTAGCTAGCTAATTAATCg  <  3:218425/133‑1 (MQ=255)
                                                                                                                             acacGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCTGAGCTGTCCCTTTCAGTGCAgg                                       <  2:339804/91‑1 (MQ=255)
                                                                                                                             acacGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCTGAGCTGTCCCTTTCAGTGCAgg                                       >  1:339804/1‑91 (MQ=255)
                                                                                                                              |                                                                                                                              
GTTCTTCGACAACGTGGTTATCGCCGGTGTGGTAACGGTCGGGCTGATGGTCGCCTTCTTTGCCGGTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCTGAGCTGTCCCTTTCAGTGCAGGCCAATCGAATATACATGATTAGCTAGCTAATTAATCG  >  ACB122/1309316‑1309568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑