Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB122994,2350TG59.3% ‑5.5 / 11.8 27R54R (CGT→CGG) CJ019_00938hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (3/8);  new base G (0/16);  total (3/24)
Fisher's exact test for biased strand distribution p-value = 5.64e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.79e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGTGAATTTGGTGC  >  ACB122/994101‑994365
                                                                                                                                      |                                                                                                                                  
ggggtggATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTg                                                                                                                                   <  4:323050/134‑1 (MQ=255)
    tggATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTgggc                                                                                                                                >  3:183770/1‑133 (MQ=255)
      gATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCggggggtcg                                                                                                                             >  6:221615/1‑131 (MQ=255)
           ggCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGGCTCcagcag                                                                                                                        >  4:319229/1‑136 (MQ=255)
              cGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGAt                                                                                                                     >  7:168671/1‑136 (MQ=255)
                             gCTCGCGGAACCGCGCGGCCAGGCCGCCCAGCCGATTCTGGCGGCCGCGGGGCAGGGTGTGGGCGAGGCGCAGTGGCTGCTGGGGAAGATCCGGCGGGATGGGCGTGGCTTCCAGCTGGATGCCACGGTTGCCCgg                                                                                                      <  8:334950/136‑1 (MQ=255)
                                     aaaCTCCCGGCTAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGGTGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGtt                                                                                              <  6:338383/136‑1 (MQ=255)
                                        cTCCGGGCACGGCGGCCCAGGGTATTGTGGCGGCTGGGGGGCAGGGTGGGGTCGAGGCGCCGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCATTGGTTCgg                                                                                           <  8:168671/136‑1 (MQ=255)
                                               caggcccgccCGGGCGTTGGGGGGGGCTGCGGGGCTGGGGGGGGGCGCGGCGCTGTGGCTGCGGGGGCAGATCCTGCGTTATGGGGGGGGCACCCCGCTGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCt                                                                                    <  8:142623/131‑1 (MQ=255)
                                               cacggCCGCCCTGGGGATTCGGGGGGCAGGGGGGCTGGGGGGGGTGGAGGCGCATTTGCGGGTGGGCCATATCCTGGGGGTGGGGCGGGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCt                                                                                    <  6:49068/133‑1 (MQ=255)
                                               cacggCCGCCCCGGCTATTGGGGCGGCTGGGGGGCTGGGGGGGGGCGAGGCGCCGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGGGGCACCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCt                                                                                    <  3:278967/133‑1 (MQ=255)
                                               cacgcccgccCCGGCTATTGGGGCGGCAGGGGGGCTGGGGGGGGGCGAGGCGCATTTGCTGCGGGGGCAGAGCCTGCTGGAGGGGCGGGGAAGCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCt                                                                                    <  4:147231/131‑1 (MQ=255)
                                               cAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGAGGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCt                                                                                    <  4:250749/136‑1 (MQ=255)
                                                aaGGCCGCCCCGGCGAGTCGGGCGGCTGGGGGGCTGGGTGGGGGCGCGGCGCAGTTGGTGCTGGGGCAGAGCCGGCTGGAGGGGCGGGGCATCCAGCTGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCt                                                                                    <  2:62211/135‑1 (MQ=255)
                                                                               ggCTGGGGGTGGGCGCGGCGCTGTTGCGGCTGGGGCAGATCCTGCGGGTTGGGGGGGGCATCCAGCTGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACAt                                                    <  6:163520/136‑1 (MQ=255)
                                                                               ggCAGGGGGGGGTGGAGCGGCTGTTGCTTCGGGGGACTAGCGTGCTGGAGGGGCGGGGCATCCGGCTGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACAt                                                    <  4:245364/136‑1 (MQ=255)
                                                                                     gggtgGGCGCGGGGCTGTGGCGGCGGGGGATGTTCGTGCTGGTTGGGGGTGGCAACCTGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCgg                                              <  4:212730/134‑1 (MQ=255)
                                                                                     gggtgGGCGCGGCGCAGGTGCGGCTGGGGCAGAGCCTGCTGGATGGGCGTGGCAGCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCgg                                              <  4:99064/134‑1 (MQ=255)
                                                                                     gggtgGGCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGAAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCgg                                              <  8:270756/134‑1 (MQ=255)
                                                                                          ggcGAGGCGCAGGTGCTGCTGGGGCAGATCCGGCGGGAGGGGCGGGGCAGCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGtt                                         <  8:198751/134‑1 (MQ=255)
                                                                                          gTCGAGGCGCAGTTGCGGCTGGGGCAGAGCCTGCTGGATGGGCGGGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGtt                                         <  4:95881/136‑1 (MQ=255)
                                                                                           gcgcggCGCTGTGGCGGCGGGGGCAGAGCCTGGGGGAGGGCCGGGGCAGCCAGCTGGATGCCACGGGTGCCCGGCGTTGGGTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGtt                                         <  1:236529/131‑1 (MQ=255)
                                                                                                              ggggCAGATCGGGCTGGTGGGGCGGGCCACCCAGCTGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGt                     <  7:2246/136‑1 (MQ=255)
                                                                                                              ggggCAGATCGGGCGGGTGGGGCGGGCAACCCCGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGt                     <  4:187062/136‑1 (MQ=255)
                                                                                                               gggCAGCGCCTGGTGGAGGGGCGGGTCACCCAGCCGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGt                     <  1:5204/135‑1 (MQ=255)
                                                                                                                  ctgATCGTGCGGGCGGGGCGGGGCATCCTGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCg                 <  4:97594/134‑1 (MQ=255)
                                                                                                                        gtgctgGAGGGGCGGGGCACCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGTGa           <  3:190046/135‑1 (MQ=255)
                                                                                                                                 gggCGGGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGTGAATTTGGTGc  <  3:19931/136‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                  
GTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGTGAATTTGGTGC  >  ACB122/994101‑994365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑