| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 2,856,882 | 0 | C | G | 53.3% | 1.3 / 10.3 | 15 | A295A (GCC→GCG) | hdfR_4 | HTH‑type transcriptional regulator HdfR |
| Reads supporting (aligned to +/- strand): ref base C (3/4); new base G (0/8); total (3/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.69e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.54e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGCGACCTGGTGGGCGAGCCGCTCATCAACGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTACTGGTGC > ACB122/2856753‑2856994 | cAGCGACCTTGTGGGCGAGCCGCTCATCAACGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGGCgcgggg > 3:278109/1‑131 (MQ=255)cAGCGACCTGGTGGGCGAGCCGCTCATCAACGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGcgggcggggggc > 8:308856/1‑132 (MQ=255) aCCTGGTGGGCGAGCCGCTCATCAACGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGGCCGCGGGCCGGc > 7:23818/1‑136 (MQ=255) ggtggtGCGGGCCCGGCGGGGGCACCCGCTGTTCCACACGCACCTGACGCGCAAGGGCGGGCACCAGGCGCGCGGGGTGTGGCCGCGGGCCGGCGCGCCGGCCCGTCAGCTGCTCGACAACTGCTTcgccgccgc < 8:70302/135‑1 (MQ=255) cGGGGGCACCGGCGGTTCCACCCTCACCGGCCGCTCCCGGCCGCGCCCCCGCCGGGCGGGGGGGGGCCGCGGGCGGGCGCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGt < 6:325009/136‑1 (MQ=255) cGGGGGCACCCGCTGTGCCACCCTCCCCTGCCGCGCAAGGGCGGGCACCAGGCGCGCGGGGTGTGGCCGCGGGCGGGCGCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGt < 5:114967/136‑1 (MQ=255) cGCTGTGCCACACCCCCCTGCCGCTCACGGGCGGGCCCCCGGCGCGGGGGGGGGGGCCGCGGGCGGGCGCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCggtggt < 3:137400/136‑1 (MQ=255) tccaccCTCACCTCACGCTCCGGGGGGGGCACCCGGCGGGGGGGGTGTGGCGGGGGGCGGGCGCGCCGGCCGGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGaaa < 1:6640/133‑1 (MQ=255) cacaCTCCCGTGCCGCTCAGGGGCGGGCACCAGGCGGGCTGGGTGTTGCCGCGGGCGGGCGCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAg < 1:65384/134‑1 (MQ=255) cACCTGACGCGCAGGGGCGGGCCCCCGGCGGGCGGGGGGTGGCCGCGGGCGGGAGCGCCGGCCGGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATc < 7:44120/136‑1 (MQ=255) atgggcgggcACCTGCCGGGGTGTGTGGGGCCGCGGGCGGGCGGGCGGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTAt < 4:305821/132‑1 (MQ=255) cgggcgggcACCCGGCGCGCTGGGGGGTGCCGCGGGCCGGCGCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATcc < 1:51708/134‑1 (MQ=255) ccAGGCGCGCGGGGGGGTGCGGCGGGCCGGCGCGCGGGCCGGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTACt < 1:52217/135‑1 (MQ=255) gcggggtGGGTGTGGCCGGGGGCGGGCGGGCGGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTACTGGTGc < 3:204109/130‑1 (MQ=255) gcgcTGGGTGTTGCCGCGGGCCGACTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTcgcc < 7:115144/61‑1 (MQ=255) gcgcTGGGTGTTGCCGCGGGCCGACTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTcgcc > 8:115144/1‑61 (MQ=255) | CAGCGACCTGGTGGGCGAGCCGCTCATCAACGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTACTGGTGC > ACB122/2856753‑2856994 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |