Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,440,3630AC61.9% ‑5.5 / 10.3 23A24A (GCA→GCC) CJ019_03266hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (4/4);  new base C (13/0);  total (19/4)
Fisher's exact test for biased strand distribution p-value = 1.17e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.70e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGATCA  >  ACB122/3440230‑3440497
                                                                                                                                     |                                                                                                                                      
ggtggtAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGc                                                                                                                                      >  3:32304/1‑136 (MQ=255)
       gCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCCCGGGCGGCGCCCCGGCCGCCgggggg                                                                                                                               >  6:6135/1‑134 (MQ=255)
                          cAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCGGGCGGCGCCCCGGCAGCCTCGGGGTGGCGGGCCCACCCCta                                                                                                              >  1:92584/1‑134 (MQ=255)
                           aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGCGGCGCCCGGGCCGCCGGGGGGTGGGCGGCCCCCGCCgat                                                                                                             >  2:59441/1‑134 (MQ=255)
                               cccTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCCCCGTGGCGGCGCCCGCGCGGGCGGGGGGTGGCGGGCCCACGCCTCTAATgcg                                                                                                       >  7:95979/1‑136 (MQ=255)
                                              cgcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGCGGCGCCCGGGCCGCCGGGGGGTGGGCGGCCCACGCCGATAAGGCGCCGCGCGCGGCTCa                                                                                         >  1:264429/1‑135 (MQ=255)
                                               gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCCCCCGGGGGGCGGCGCGGCCGCCGGGGGGTGGGCGGGCCCCCCCGCTAAAGCGCCGCTCGCTGATCAg                                                                                        >  2:327505/1‑135 (MQ=255)
                                               gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCTGGCGGCGGCCGGGCCGCCGGGGGGGGGGCGGCCCCCCCCCATAACGCGCCGCACGCGGATAAgg                                                                                       >  6:40642/1‑136 (MQ=255)
                                                 tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGGAGCCCGGGGGTGGCCGGCCCACGGCGATAAGTCGCCGCGCGCTGCTCAgggc                                                                                     >  8:324138/1‑134 (MQ=255)
                                                 tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCGGGGCGGTGGCAGGGCCCCCCCCATAAAGCGCCCCTCGGGGATCAGGcc                                                                                     >  3:301862/1‑136 (MQ=255)
                                                 tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGTGTGGCCGGCCCACGCCGATAAGGCGCCGCGCGCGGCTCAGGcc                                                                                     >  5:154162/1‑136 (MQ=255)
                                                            cAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGTGTGGGCGGCCCCCCCCGATAAGGCGCAGCTCGCGGCTCAGGCCGCTGACTGCgg                                                                          >  7:172262/1‑136 (MQ=255)
                                                            cAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCCCTGGCGGCGCCCGGGCCGCCCGGGGGTGGCCGGCCAACGCCCAAAAAGCGCCCCTCGCGGCTCAGGCCGCTGACttag                                                                           >  2:23512/1‑133 (MQ=255)
                                                                    gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTTCTGATGGCCCCCACCGCGGGCGGCGCGCGGGCCGCCTGGGTGTGGGCGGCCAACCCCACTAACGCGCCGCTCGGTGCTGAGGCCGGGGGCTTGGGCGAGCAgg                                                                  >  4:299170/1‑135 (MQ=255)
                                                                    gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCTGGGTGTGGCCGGCCAACCCCTATAAGGCGCAGCTCGCGGATCAGGCCGATGACTTGGGCACGCAgg                                                                  >  7:140461/1‑135 (MQ=255)
                                                                    gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCCCCGGGGTGTGGGCGGCCCACGCCGATAAGGCGCAGCGCGCGGAGCAGGCCGCTGCCTGCGGCAAGCAGt                                                                  >  4:168894/1‑136 (MQ=255)
                                                                         ggCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGTGTGGCCGGCCCCCGCCGAGAAGGCGCCGCGCGCTGATCCGGCCGCTGGCGTCGGCAAGCAGGTGGCg                                                             >  7:77227/1‑136 (MQ=255)
                                                                                 gCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCCCGCGGGCGGGGCCCGGGCGGCCGGGGGGTGGGGGGGCCACGCCCATAATGCGGCGCGCGCTGAGCAGGCCGCGGCCGTAGGCCAGCAGGTGGGGGGGGAggg                                                     >  4:278881/1‑135 (MQ=255)
                                                                                       gggCCGTGGCGCTGCTGCGGCCCGCCAGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTTGCAGGCCATCGCCTATAAGTCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCTGAGAAGTATcgcgc                                               <  8:5629/134‑1 (MQ=255)
                                                                                                         gggCCGCCCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGga                             <  7:299929/135‑1 (MQ=255)
                                                                                                            ccgccCGGGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGaa                          <  4:124505/136‑1 (MQ=255)
                                                                                                                       ggcggcGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTgcagcag               <  4:32304/136‑1 (MQ=255)
                                                                                                                          ggcgCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGcagcagcag            >  3:191040/1‑136 (MQ=255)
                                                                                                                                     aGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGATCa  >  2:295548/1‑135 (MQ=255)
                                                                                                                                     |                                                                                                                                      
GGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGATCA  >  ACB122/3440230‑3440497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑