| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 3,995,418 | 0 | T | G | 52.9% | 3.8 / 11.4 | 17 | intergenic (+90/‑44) | dmoA_3/allE | Dimethyl‑sulfide monooxygenase/(S)‑ureidoglycine aminohydrolase |
| Reads supporting (aligned to +/- strand): ref base T (0/8); new base G (0/9); total (0/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.66e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCACGTTGGACGTCAGGTCAGCATTCAGTGATCAAATACCGGGTA‑TTCCAACATTTAATTTGTGGATGCCACGCACTCGACCCTATTCTGTGGCCACATCCCCTGCGCCGCACCGGCCGGTGCGGTTCAAGGCATGGGGAGAGAACAACAAGTCGAGATCGTCCATGTCGAACCATTCATACTTCGCCCCCCACGGTGGGCACCCGGCTCAAACCGAGCTGCTGACTGACCGTGCCATGTTCACCGAAGCCT > ACB122/3995297‑3995549 | ggCACGTTGGACGTCAGGTCAGCATTCAGTGATCAAAAACCGGGTA‑TTCCAACATTTAATTTGTGGATGCCACGCACTCGACCCTATTCTGTGGCCACATCCCCTGCGCCGCACCGGCCGGTGCGGTTCAAGGCAt < 8:114667/136‑1 (MQ=255) aggtcaggtacgCATTATGTGATC‑AATACCCGGTATTTCCAACATTTAATTTGGGTATGCCCCGCACTCCACCATATTCTGGGGCCACAGCCCCTGCGCCGCACCGGCGGGGGCGGTTCAAGGCATGGGGTGAGaa < 6:260100/134‑1 (MQ=255) aCGTCAGGTCAGCATTCAGTGATCAAATACCGGGTA‑TTCCAACATTTAATTTGTGGATGCCACGCACTCGACCCTATTCTGTGGCCACATCCCCTGCGCCGCACCGGCCGGTGCGGTTCAAGGCATGGGGAGAGaa < 5:130278/136‑1 (MQ=255) cGTCAGGTCAGCATTCAGTGATCAAATACCGGGTA‑TTCAAACATTTAATTTGTGGATGCCACGCACTCGACCCTATTCTGTGGCCACATCCCCGGCGCCGCACCGGCCGGTGCGGTTCAAGGCATGGGGAGAGaac < 4:50879/136‑1 (MQ=255) cAAATACCGGGTA‑TTCCAACATTTAATTTGTGGATGCCACGCACTCGACCCTATTCTGTGGCCACATCCCCTGCGCCGCACCGGCCGGGGCGGTTCAAGGCATGGGGAGAGAACAACAAGTCGAGATCGTCCATGt < 3:39733/136‑1 (MQ=255) cAAATACCGGGTA‑TTCCAAAATTTAAGTTGTGGATGCCACGCACTCGACCCTATTCTGGGGCCACATCCCCGGCGCCGCACCGGCGGGTGCGGTTCAAGGCATGGGGAGAGAACAACAAGTCGAGATCGTCCATGt < 5:13767/136‑1 (MQ=255) aaaaacccGGGAA‑TACAAAAATTTAATTGGTGGATCCCACGCACGCGCCCCTATTCTGGGCCCACACCCCCTGCGCCGCCCCGGCCGGGGCGGTTCAAGGCATGGGGAGAGAACAACAAGTCGAGATCGTCCATGt < 6:333002/130‑1 (MQ=255) aaaTCCGGGGTA‑TTCCAACATTTATTTTGTGGATGCCACGCACTCGACCCTATTCTGTGGCCACATCCCCTGCGCCGCACCGGCCGGTGCGGTTCAAGGCATGGGGAGAGAACAACAAGTCGAGATCGTCCATGt < 4:88374/135‑1 (MQ=255) gACCGTTTTCTGTGCCCACTGCCCCGGCGCCGCCCCGCCGGGGGCGGTTGAAGGCATGGGGAGAGAACAACAAGTCGAGATCGTCCATGTCGAACCATTCATACTTCGCCCCCCACGGTGGGCACCCGGCTCAAAc < 4:286776/136‑1 (MQ=255) cccTATTCTGTGGCCACAGCCCCGGCGCCGCACGGGCCGGGGCGGTTCAAGGCATGGGGAGAGAACAACAAGTCGAGATCGTCCATGTCGAACCATTCATACTTCGCCCCCCACGGTGGGCACCCGGCTCAAACCg < 7:76566/136‑1 (MQ=255) cccTATGGTGTGGCCACATCCCCTGCGCCGCACGGGCGGGTGCGGTTCAAGGCATGGGGAGAGAACAACAAGTCGAGATCGTCCATGTCGAACCATTCATACTTCGCCCCCCACGGTGGGCACCCGGCTCAAACCg < 4:269234/136‑1 (MQ=255) ggCCACATCCCCTGCGCCGCACCGGCCGGTGCGGTTCAAGGCATGGGGAGAGAACAACAAGTCGAGATCGTCCATGTCGAACCATTCATACTTCGCCCCCCACGGTGGGCACCCGGCTCAAACCGAGCTGctgac < 2:232394/135‑1 (MQ=255) ccagccccGGCGCCCCACCGGCCGGGGCGGTTCAAGGCATGGGGAGAGAACAACAAGTCGAGATCGTCCATGTCGAACCATTCATACTTCGCCCCCCACGGTGGGCACCCGGCTCAAACCGAGCTGCtgactgac < 2:136892/131‑1 (MQ=255) tCCCCTGCGCCGCACCGGCCGGGGCGGTTCAAGGCATGGGGAGAGAACAACAAGTCGAGATCGTCCATGTCGAACCATTCATACTTCGCCCCCCACGGTGGGCACCCGGCTCAAACCGAGCTGCTGACTGACCGt < 2:50713/135‑1 (MQ=255) ccccccggcgggGGCGGTTCAAGGCATGGGGAGAGAACAACAAGTCGAGATCGTCCATGTCGAACCATTCATACTTCGCCCCCCACGGTGGGCACCCGGCTCAAACCGAGCTGCTGACTGACCGTGCCATGTTCa < 1:328930/131‑1 (MQ=255) cgggTGCGGTTCAAGGCATGGGGAGAGAACAACAAGTCGAGATCGTCCATGTCGAACCATTCATACTTCGCCCCCCACGGTGGGCACCCGGCTCAAACCGAGCTGCTGACTGACCGTGCCATGTTCACCGAAGCCt < 6:104880/134‑1 (MQ=255) ccggGGCGGTTCAAGGCATGGGGAGAGAACAACAAGTCGAGATCGTCCATGTCGAACCATTCATACTTCGCCCCCCACGGTGGGCACCCGGCTCAAACCGAGCTGCTGACTGACCGTGCCATGTTCACCGAAGCCt < 8:37577/136‑1 (MQ=255) | GGCACGTTGGACGTCAGGTCAGCATTCAGTGATCAAATACCGGGTA‑TTCCAACATTTAATTTGTGGATGCCACGCACTCGACCCTATTCTGTGGCCACATCCCCTGCGCCGCACCGGCCGGTGCGGTTCAAGGCATGGGGAGAGAACAACAAGTCGAGATCGTCCATGTCGAACCATTCATACTTCGCCCCCCACGGTGGGCACCCGGCTCAAACCGAGCTGCTGACTGACCGTGCCATGTTCACCGAAGCCT > ACB122/3995297‑3995549 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |