Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1224,413,5530AC34.0% 59.4 / 10.5 47I7L (ATC→CTC) CJ019_04183hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (21/10);  new base C (0/16);  total (21/26)
Fisher's exact test for biased strand distribution p-value = 4.49e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CCCTGACGGGCCGCGCTACAAGGCGATCGGCAACAGCAAGGCCGTGTTCGTCGTCCGCTGGATCGGCCGGCGCATCCAACAACAACTTGAACGTCTTGCTTGAGGTTACCCATGCCCACAGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGATGGCCAACCCCCCTAAGGCTA  >  ACB122/4413424‑4413688
                                                                                                                                 |                                                                                                                                       
cccTGACGGGCCGCGCTACAAGGCGAGCGGCAACAGCAAGGCCGTGTTCGTCGTCCGCGGGAGCGCCCGGCGCATCCAACAACAACTTTAACGTCTTGCTTGAGGTTACCCATGCCCACATAAAACAAAATCGACt                                                                                                                                   <  3:309824/136‑1 (MQ=255)
             cgcTACAAGGCGATCGGCAACAGCAAGGCCGTGTTCGTCGTCCGCTGGATCGGCCGGCGCATCCAACAACAACTTGAACGTCTTGCTTGAGGTTACCCATGCCCACAGAAAACAAAATCGACTGCCCGGCATTGca                                                                                                                      >  5:304899/1‑136 (MQ=255)
              gcTACAAGGCGATCGGCAACAGCAAGGCCGTGTTCGTCGTCCGCTGGATCGGCCGGCGCATCCAACAACAACTTGAACGTCTTGCTTGAGGTTACCCATGCCCCCAGAAAACAAAATCGACTGCCCGGCATTGc                                                                                                                       >  2:315192/1‑134 (MQ=255)
               cTACAAGGCGATCGGCAACAGCAAGGCCGTGTTCGTCGTCCGCTGGTTCGGCCGGCGCATCCAACAACAACTTGACCGTCTTGCTTGAGGTTACCCATGCCCACAGAAAACAAAATCGACTGCCCGGCATTGcaca                                                                                                                    <  7:243677/136‑1 (MQ=255)
                   aaGGCGATCGGAAACAGCAAGGCCGTGTTCGGCGTCCGCTGGATCGGCCGGCGCAGCCAACAACAACTTGACCGTCTTGCTTGAGGTTACCCATGCCCACAGAAAACAAACTCGACTGCCCGGCATTGCACAAGCg                                                                                                                <  8:253321/136‑1 (MQ=255)
                          tgggaaaCAGCACGGCCGGGTGGGTCGGCCGCGGGATCGGCGGGCGCATCCAACAACAACTTCAAGGTCTTGCTTGTGGTTACCCAGGCCCACATAAAACAAACTCGACTGCCCGGCATTGCACAAGCGAATGTTc                                                                                                         <  3:327468/131‑1 (MQ=255)
                             gCAACAGCAAGGCCGTGTTCGTCGTCCGCTGGATCGGCCGGCGCATCCAACAACAACTTGAACGTCTTGCTTGAGGTTACCCATGCCCACAGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGc                                                                                                      >  4:209051/1‑136 (MQ=255)
                                  aGCATGGCCGTGTTGGTCGTCCGCGGTTTCGGCGGGCGCATCCAACAACAACTTGAAGTTCTTGCTTGAGGTAACCAATGCCCACATAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTAccc                                                                                                 <  6:121574/136‑1 (MQ=255)
                                         ccGTGTTCGTCGTCCGCTGGATCGGCCGGCGCATCCAACAACAACTTGAACGTCTTGCTTGAGGTTACCCATGCCCACAGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGc                                                                                          >  5:287255/1‑136 (MQ=255)
                                              ttCGTCGTCCGCTGGATCGGCCGGCGCATCCAACAACAACTTGAACGTCTTGCTTGAGGTTACCCATGCCCACAGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATcg                                                                                     >  5:169958/1‑136 (MQ=255)
                                              ttCGTCGTCCGCTGGAGCGGCCGGCGCATCCAACAAAAACTTGAACTTTTTGCTTGAGGATACCAATCCCCACACAAAAAAAACTCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATcg                                                                                     <  6:177214/136‑1 (MQ=255)
                                               tcgtcgTCCGCTGGATCGGCCGGCGCATCCAACAACAACTTGAACGTCTTGCTTGAGGTTACCCATGCCCACAGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATcgc                                                                                    >  6:159356/1‑136 (MQ=255)
                                               tcgtcgTCCGCTGGATCGGCCGGCGCATCCAACAACAACTTGAACGTCTTGCTTGAGGTTACCCATGCCCACAGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATcgc                                                                                    >  5:7165/1‑136 (MQ=255)
                                               tcgtcgTCCGCTGGATCGGCCGGCGCATCCAACAACAACTTGAACGTCTTGCTTGAGGTTACCCATGCCCACAGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATcgc                                                                                    >  6:318468/1‑136 (MQ=255)
                                               tcgtcgTCCGCTGGATCGGCCGGCGCATCCAACAACAACTTGAACGTCTTGCTTGAGGTTACCCATGCCCACAGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATcg                                                                                     >  2:59831/1‑135 (MQ=255)
                                                ggggggccGCTGGAGGGGCGGGCGCAAACAACAACAAGTTCACCGGATTGGTTGCGGTAACCCATCCCCCCACAAAAAAAACTCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATcgcg                                                                                   <  5:328840/130‑1 (MQ=255)
                                                cgccgcccGCGGGATCGGCCGGCGCATCCAACACAAACTTCAACGGCTTGCTTGCGGTAACCCATGCCCACACAAAACAAACTCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATcgc                                                                                    <  8:44819/129‑1 (MQ=255)
                                                 gtcggcCGGTGGAGCGGCCGGCGCAGCCAACAACAACTTGAACGGCTTGCTTGCGGTAACCCATGCCCAAATAAAACAAACTCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATcgcg                                                                                   <  2:228147/135‑1 (MQ=255)
                                                       cGCTGGATCGGCCGGCGCATCCAACAACAAGTTGAACGTCTTGCTTGCGGTTCCCCCTGCCCACCCAAAACAAACTCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCcgcg                                                                            <  6:142479/136‑1 (MQ=255)
                                                              gcggccgggGCATCCAACAACAACTTGACCGTCTTGCTTGAGGTTACCCATGCCCACAGAAAACAAACTCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTc                                                                     <  6:150767/135‑1 (MQ=255)
                                                                 gccggcGCTTCAAACAACAACTTGACGGTCTTGCTTCTGGTTACCCATGCCCACAGAACACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGc                                                                  <  4:26093/136‑1 (MQ=255)
                                                                                  caacTTGAACGTCTTGCTTGAGGTTACCCATGCCCACAGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCa                                                  >  2:286624/1‑135 (MQ=255)
                                                                                   aacTTGAACGTCTTGCTTGAGGTTACCCTGCCCCACACAAAAAAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCAcc                                                <  5:134628/136‑1 (MQ=255)
                                                                                   aaattcaaCGTCTTGCTTGCGGTACCCCAGGCCCACACAAAACAAACTCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCAcc                                                <  5:175697/130‑1 (MQ=255)
                                                                                        gAACGTCTTGCTTGAGGTTCCCCATGCCCACAGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCAccgcc                                             <  3:71991/134‑1 (MQ=255)
                                                                                        gAACGTCTTGCTTGAGGTTACACATGCACACAGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCAccgccg                                            >  7:272601/1‑135 (MQ=255)
                                                                                          aCGTCTAGCTTGAGGTTACCCATGCCCACACAAAACAAAATCGACTGCCCGGCATTGCACAACCGAATCTTCGGCTACCCGTACGGCGATCGCGTACCGCGTACCGTCCGCATGCTGGTTACCGTCACCGCCGAAc                                         >  7:45823/1‑136 (MQ=255)
                                                                                                tgcttgATTTTCCCCTTCCACACATAAAACAAAATCGACTTCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCTAGTTACGCTTACTTTCATCATGCTTTTTAACGTCACCGCCGAACGCAt                                     <  2:276057/134‑1 (MQ=255)
                                                                                                tgcttgAGGTTACCCATGCCCACAGAAAACAAACTCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCa                                                                 <  3:294499/106‑1 (MQ=255)
                                                                                                tgcttgAGGTTACCCATGCCCACAGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCa                                                                 >  4:294499/1‑106 (MQ=255)
                                                                                                         taaCCCATCCCCACATAAAACAAACTCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCg                          <  6:169958/134‑1 (MQ=255)
                                                                                                           aCCCATGCCCACAGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTc                           >  8:270097/1‑133 (MQ=255)
                                                                                                              cATGCCCACAGAAAACAAAATCGACTGCCCGGCATTGCACAAGAGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGATg                     >  8:58401/1‑136 (MQ=255)
                                                                                                               aTGCCCACAGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGATgg                    >  4:129701/1‑136 (MQ=255)
                                                                                                                  cccACAGAAAAAAAACTCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGATGGCCa                 <  3:89814/136‑1 (MQ=255)
                                                                                                                  cccACAAAAAAAAAACTCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGATGGcc                  <  6:7165/135‑1 (MQ=255)
                                                                                                                    cacaGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATcg                                                                                     >  1:226433/1‑66 (MQ=255)
                                                                                                                    cacaGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATcg                                                                                     <  2:226433/66‑1 (MQ=255)
                                                                                                                    cacaGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGATGGCCacc               >  7:76227/1‑134 (MQ=255)
                                                                                                                    cacaGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGATGGCCAAc               >  5:267627/1‑136 (MQ=255)
                                                                                                                    cacaGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGATGGCCAAc               >  4:43001/1‑136 (MQ=255)
                                                                                                                     acaGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGATGGCCAAcc              >  7:243649/1‑136 (MQ=255)
                                                                                                                       aGAAAACAAACTCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGATGGCCAAcccc            <  8:277066/136‑1 (MQ=255)
                                                                                                                       aGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTTACCGCCGAACGCATCCCTGGTGTCGAGATGGCCAAcccc            <  6:104475/136‑1 (MQ=255)
                                                                                                                        gAAAACAAACTCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGATGGCCAAcccc            <  2:87759/135‑1 (MQ=255)
                                                                                                                        caaaaaaaaATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGATGGCCAAcccc            <  2:206197/134‑1 (MQ=255)
                                                                                                                        caaaaCAAACTCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGATGGCCAAcccc            <  1:58768/134‑1 (MQ=255)
                                                                                                                                 aTCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGATGGCCAACCCCCctaaggcta  <  4:178854/136‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                       
CCCTGACGGGCCGCGCTACAAGGCGATCGGCAACAGCAAGGCCGTGTTCGTCGTCCGCTGGATCGGCCGGCGCATCCAACAACAACTTGAACGTCTTGCTTGAGGTTACCCATGCCCACAGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGATGGCCAACCCCCCTAAGGCTA  >  ACB122/4413424‑4413688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑