Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1224,500,2270AC46.4% 4.4 / 10.0 28G56G (GGT→GGG) cysG_1Siroheme synthase
Reads supporting (aligned to +/- strand):  ref base A (12/3);  new base C (9/4);  total (21/7)
Fisher's exact test for biased strand distribution p-value = 6.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GGCAGGCTAAGGGCCTGCGCATCGGCCGACACCTGGGCATTCAGCCCAGGGTCGTCAGTCGCCGCGATCACCAGCCGGCAACCGACCAGGTCGGCTGCCTGATAGCCACGCACCAGCACCTCACCGCCACCTTCCCGGGCCAACGCGGCCAACTGGCCGTCGACGTCCGGCGCCACCACGCGCAGCACGCCGCCGGCATCGGCCAGCAGGCGCGCCTTGCGCAAGGCGATCTCACCGCCACCGACGACCAGCACGCGGCC  >  ACB122/4500099‑4500358
                                                                                                                                |                                                                                                                                   
ggCAGGCTAAGGGCCTGCGCATCGGCCGACACCTGGGCATTCAGCCCAGGGTCGTCAGTCGCCGCGCTCACCAGCCGGCCACCGACCAGGTCGGCTGCCTGATAGCCACGCCCCAGCCCCTCACCGCCACCTTccc                                                                                                                              >  3:86227/1‑136 (MQ=255)
     gCTAAGGGCCTGCGCATCGGCCGACACCTGGGCATTCAGCCCAGGGTCGTCAGTCGCCGCGCTCACCAGCCGGCCACCCACCAGGTCCGCGGCCTGATAGCCCCGCCCCCGCCCCTCCCCGCCCCCGTCCCGCGcc                                                                                                                         >  5:58683/1‑136 (MQ=255)
                  gcATCGGCCGACACCTGGGCATTCCGCCCAGGGTCGTCAGTCGCCGCGATCACCAGCCCGCAACCCGCCAGGTCGGCAGCCGGATAGCCACGCACCAGCCACTCCCCCCCCCCCTCCCGCGCCCACGCGGccccct                                                                                                            >  4:2788/1‑132 (MQ=255)
                  gcATCGGCCGACACCTGGGCATTCAGCCCAGGGTCGTCAGTCGCCGGGATCACCAGCCGGCCACCGACCCGGTCGGGTGCCAGAATGCCCCGCACCAGCACCTCCCCGCCACCATCCCCGGCCCACCCGGccca                                                                                                              >  3:49240/1‑132 (MQ=255)
                  gcATCGGCCGACACCTGGGCATTCAGCCCAGGGTCGTCAGTCGCCGCGATCCCCAGCCGGCAACCGCCCAGGTCGGCTGCCTGCTAGCCACGCACCAGCCCCGCACCCCCCCCTTCCCCGGCCAACCCGGCCAACt                                                                                                            >  4:282100/1‑136 (MQ=255)
                  gcATCGGCCGACACCTGGGCATTCAGCCCAGGGTCGTCAGTCGCCGCGATCACCAGCCGGCAACCGCCCAGGTCGGCTGCCTGATAGCCACGCACCAGCACCTCACCGCCACCTTCCCGGGCCAACGCGGCCAACt                                                                                                            >  4:130991/1‑136 (MQ=255)
                  gcATCGGCCGACACCTGGGCATTCAGCCCAGGGTCGTCAGTCGCCGCGATCACCAGCCGGCAACCGCCCAGGTCGGCAGCCTGATAGCCACGCACCCGCCCCTCCCCGCCACCGTCCCGGGCCCCCGCGGCCa                                                                                                               >  2:60766/1‑133 (MQ=255)
                  gcATCGGCCGACACCTGGGCATTCAGCCCAGGGTCGTCAGTCGCCGCGAAAACCAGCCGGCCACCGCCCAGGTCGGCAGCCTGATAGCCAAGCACCAGCACCTCCCCCCCCCCTTacccggccaacccggcca                                                                                                               >  1:105484/1‑133 (MQ=255)
                         ccGACACCTGGGCATTCAGCCCAGGGTCGTCAGTCGCCGCGCTCACCAGCCGGCAACCGCCCAGGTCGGCTGCCTGATAGCCACGCACCCGCACCTCACCGCCCCCTTCCCGGGCCCACGCGGCCAACTGGCCGTc                                                                                                     >  7:29095/1‑136 (MQ=255)
                              acCTGGGCATTCAGCCCAGGGTCGTCAGTCGCCGCGATCACCAGCCGGCAACCGACCAGGTCGGCTGCCTGATAGCCACGCACCCGCCCCTCACCCCCCCCATCCCCGGCCAACGCGGCCCACAGGCCCTCGCCGt                                                                                                >  6:269716/1‑136 (MQ=255)
                                 tGGGCATTCAGCCCAGGGTCGTCAGTCGCCGCGAGCACCAGCCGGCAACCGACCAGGTCGGCTGCCTGATAGCCACGCACCAGCACCTCACCGCCACCTTCCCGGGCCAACGCGGCCAACTGGCCGTCGACGTCCg                                                                                             <  7:265565/136‑1 (MQ=255)
                                                    cgtcAGTCGCCGCGCTCACCAGCCGGCAACCGACCAGGTCGGCTACCTGATAGCCACGCCCCCGCACCTCACCGCCCCCTTCCCGGGCCAACGCGGCCAACTGGCCGTCGACGTCCGGCGCCACCCCGcgccgccc                                                                          >  7:283037/1‑134 (MQ=255)
                                                         gTCGCCGCGATCACCAGCCGGCAACCGACCAGGTCGGCTGCCTGATAGCCACGCCCCAGCACCTCACCGCCCCCTTCCCGGGCCCACGCGGCCAACAGGCCGTCGACGTCCGGCGCCCCCCCGCGCCGCAcgccgc                                                                     >  7:183481/1‑136 (MQ=255)
                                                         gTCGCCGCGATCACCAGCCGGCAACCGACCAGGTCGGCTGCCTGATAGCCACGCACCAGCACCTCACCGCCAcc                                                                                                                                   <  7:80578/74‑1 (MQ=255)
                                                         gTCGCCGCGATCACCAGCCGGCAACCCACCAGGTCGGCTGCCTGATAGCCACGCACCAGCACCTCACCGCCAcc                                                                                                                                   >  8:80578/1‑74 (MQ=255)
                                                                        cgCCGTCAACCGCCCCGGTCGGAGGCCTGATCGCCACGCCCCAGCACCGCCCCGCCACCTTCCCGGGCCAACGCGGCCACCGGGCCGTCGACGTCCGGCGCCACCACGCGCAGCACGCCGCCGGCATCGGCcagca                                                      <  8:166477/135‑1 (MQ=255)
                                                                        ccccGCCAACCGCCCAGGGCGGCTGCCTGATCGCCCCCCCCCACCACCTCCCCCCCCCCTCCCCGGCCCAACGCGCCCAACTGGCCGGCGACGTCCGGCGCCACCACGCGCAGCACGCCGCCGGCATCGGCcagca                                                      <  4:271443/134‑1 (MQ=255)
                                                                                       aGGTCGGCTGCCTGATAGCCACGCACCAGCACCTCACCGCCACCTTCCCGGGCCAACGCGGCCAACTGGCCGTCGCCGTCCGGCGCCACCCCGCGCAGCACGCCGCCGGCATCGGCCAGCAGGCGCGCCGGgcgc                                        >  5:230937/1‑135 (MQ=255)
                                                                                           cGGCTGCCTGATAGCCACCCACACCCACCTCACCCCCCCCTACCCGGCCCACCGCGGCCAACTGGCCGTCGACGTCCGGCGCCACCACGCGCAGCACGCCGCCGGCATCGGCCAGCAGGCGCGCCTTGCGCAAGGc                                   <  3:282100/136‑1 (MQ=255)
                                                                                               tGCCTGATAGCCACGCACCCGCACCTCACCGCCACCGTCCCGGGCCCCCGCGGCCAACTGGCCGGCGCCGGCCGGCGCCCCCACGCGCCGCCCGCCGCCGGCAGCGGCCAGCAGGCGCGCCGTGCGCCCGGCGagc                               >  5:263233/1‑134 (MQ=255)
                                                                                               tGCCTGATAGCCACGCACCAGCACCTCACCGCCCCCTTCCCGGGCCAACGCGGCCAACTGGCCGTCGACGTCCGGCGCCCCCACGCGCAGCACGCCGCCGGCCTCGGCCCGCAGGCGCGCCTTGCGCACGGCGAtc                               >  3:22365/1‑136 (MQ=255)
                                                                                                     aTACCCCCGCACCCCCCCCTCCCCCCCCCATCCCGGGGCCACGGCGGCAAAGGGGCGGGCGACGTCGGGCGCCACCACGCGCAGCACGCCGCCGGCATCGGCCAGCAGGCGCGCCTTGCGCAAGGCGATCTCAc                           <  1:266113/134‑1 (MQ=255)
                                                                                                      tAGCCACGCACCAGCACCTCACCGCCACCTTCCCGGGCCAACGCGGCCAACTGGCCGTCGACGTCCGGCGCCACCACGCGCCGCACGCCGCCGGCATCGGCCAGCAGGCGCGCCTTGCGCAAGGCGCTCTCCCCGc                        >  6:237813/1‑136 (MQ=255)
                                                                                                             ccACCAGCACCTCACCGCCCCCTTCCCGGGCCACCGCGGCCAACTGGCCGTCGACGTCCGGCGCCACCACGCGCAGCACGCCGCCGGCATCGGCCAGCAGGCGCGCCTTGCGCAAGGCGATCTCACCGCCACcgac                 <  7:269490/135‑1 (MQ=255)
                                                                                                              cACCAGCACCTCACCGCCACCTTCCCGGGCCAACGCGGCCAACTGGCCGTCGACGTCCGGCGCCACCACGCGCAGCACGCCGCCGGCATCGGCCAGCAGGCGCGCCTTGCGCAAGGCGCTCTCACCGCCCCcgac                 >  2:165973/1‑135 (MQ=255)
                                                                                                               aCCAGCACCTCACCGCCACCTTCCCGGGCCAACGCGGCCAACTGGCCGTCGACGTCCGGCGCCACCACGCGCAGCACGCCGCCGGCATCGGCCAGCAGGCGCGCCTTGCGCAACGCGATCTCACCGCCACcgacga               >  3:41554/1‑136 (MQ=255)
                                                                                                                      ccTCACCGCCACCTTCCCGGGCCAACGCGGCCAACTGGCCGTCGACGTCCGGCGCCACCCCGCGCAGCCCGCCGCCGGCATCGGCCAGCAGGCGCGCCTTGCGCCAGGCGCTCTCACCGCCACCCACGcccccca         >  2:121228/1‑131 (MQ=255)
                                                                                                                            cGCCACCTTCCCGGGCCAACGCGGCCAACTGGCCGTCGACGTCCGGCGCCACCACGCGCAGCACGCCGCCGGCATCGGCCAGCAGGCGCGCCTTGCGCCAGGCGCTCTCCCCGCCCCCGCCCCCCCGCCCGCGgcc  >  5:216397/1‑136 (MQ=255)
                                                                                                                                |                                                                                                                                   
GGCAGGCTAAGGGCCTGCGCATCGGCCGACACCTGGGCATTCAGCCCAGGGTCGTCAGTCGCCGCGATCACCAGCCGGCAACCGACCAGGTCGGCTGCCTGATAGCCACGCACCAGCACCTCACCGCCACCTTCCCGGGCCAACGCGGCCAACTGGCCGTCGACGTCCGGCGCCACCACGCGCAGCACGCCGCCGGCATCGGCCAGCAGGCGCGCCTTGCGCAAGGCGATCTCACCGCCACCGACGACCAGCACGCGGCC  >  ACB122/4500099‑4500358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑