| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 5,262,909 | 0 | A | C | 55.6% | 6.9 / 14.6 | 20 | intergenic (+9/+27) | uacT_2/radA | Uric acid transporter UacT/DNA repair protein RadA |
| Reads supporting (aligned to +/- strand): ref base A (2/6); new base C (8/2); total (10/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.36e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.10e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTGCGTGATGGGGACTACTTCAAGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTGGGCACGAT > ACB122/5262775‑5263034 | cTGCGTGTGGGGGACTACTTCAAGGATGGCAAGCTGTTCGATGCCGAGTGTAACTAAGTGCCGATGCTGGACCTGGACGAGCACGGCAATGAAACGCTAAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAg < 4:204174/136‑1 (MQ=255)cTGCGTGATGGGGACTACTTCAAGGATGGCAAGCTGGTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAg < 8:233908/136‑1 (MQ=255) actTCAAGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCGCGGCCGTTGCCGAGCACTGACGGGTTGCCGGCGCGCGCGTgctcgc > 3:186451/1‑136 (MQ=255) aTGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGGACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCCGGCGCGCGCTTGCTCGCGCCGGcgc > 1:13877/1‑133 (MQ=255) aTGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCCGGAGCGAGCTTGCTCGCGCCTGCAc > 2:54373/1‑135 (MQ=255) aaGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCCGGCGCGCGCTTGCTCGCTCCTGCACGCAAtt > 4:24251/1‑136 (MQ=255) aaGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCCGGCGCGCGCATGCTCGCGACTGCACCCGAtt > 3:99645/1‑136 (MQ=255) aaGCTGTTCGATGCCGAGGCTAACGAAGAGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGGAAGACCCAGCTTGGTCTCCCCAGGACGCTaa > 3:48875/1‑134 (MQ=255) aGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCCCTCATTCTCCGTCCTGCACGCTAt > 6:318241/1‑134 (MQ=255) gATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCCGGCGCCGGCTTGCTCGCTCCAGCCCGCGATTCGCACAATg > 6:116479/1‑136 (MQ=255) gcggcgggTACGGAAGGGCCGATGCTGCAACTGGCCGCGCCCGGCATTGAAAGGGGCCGGCGCCCGGCGGTGGCCTAGCACTGAGGGGTTGCAGGAGCCAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCAt < 8:181848/131‑1 (MQ=255) cGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCCCGCGCGCGCATGCTCGCGCCGGCCCGCGATTAGAACAAAAGAACCACCGCCAGTTGCAGCCGCGGAACCGCAAAAACCAGCAAc > 3:143501/1‑136 (MQ=255) aCGGCCGTTGCCGAGCACTGAGGGGTTGCAGGAGCGCGCTGGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGgcg < 6:276489/136‑1 (MQ=255) ccGTTGCCGAGCACTGACGGGTTGGCGGCGCGCGGTTGGTCTGTGCTGCACGCGATTCGTTCAATGACTGCAGCCGCGGTTGCCGCCGGGGGACGGCACTCACCAGCCCCCCCGCACGCGGGTCTGTCGgggccgt > 6:335894/1‑133 (MQ=255) ccGCGCACTGCGGGGTTGCTGGAGCGAGCTTGCTCGCCCCGGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCtt < 6:74251/136‑1 (MQ=255) gAGCTCTGCGGGGTTGCAGGCGGGACCTTGGTCGCGCCGGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTgg < 3:262625/136‑1 (MQ=255) gACGGGTTCCCGGAGCGAGCTTGCGCGCGCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTGGGCACGAt < 3:326545/136‑1 (MQ=255) gACGGGTGGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTGGGCACGAt < 3:90865/136‑1 (MQ=255) ccggggTGGCCGGCGCGCGGTTGCGCGCGCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTGGGCACGAt < 6:253259/133‑1 (MQ=255) cggggTGGCTGGCGCGCGTTGGCGCGCCCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTGGGCACGAt < 7:45829/133‑1 (MQ=255) | CTGCGTGATGGGGACTACTTCAAGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTGGGCACGAT > ACB122/5262775‑5263034 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |