| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 5,379,210 | 0 | A | C | 55.6% | 3.2 / 14.5 | 18 | intergenic (+35/‑28) | glcA/lldD | Glycolate permease GlcA/L‑lactate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (3/5); new base C (10/0); total (13/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.54e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.10e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACCGTCAAGCACAGCCTGTTCTTCGCCACCATCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGCCGTCGCGCGCCCGG‑CACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCAAGCTGCCCCCCTTCCTGTTCCACTACGCTGACGGCGGCGCCTACGCCGAGCACACCCT > ACB122/5379083‑5379341 | aCCGTCAAGCACAGCCTGTTCTTCGCCACCATCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCCCCGTCCCGCGCCCCg‑cccgcccccc > 5:309330/1‑133 (MQ=255) ttcGCCACCATCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGCCGTCGCGCGCCCGG‑CCCGCCCCCCAGCCACCCTGCGCGAATCCa > 2:21723/1‑135 (MQ=255) ccaccaTCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGCCGTCGCGCGCCCGG‑CCCTCCCCCAAGCCAACCGGCGAGAATCCATGGTc > 7:113425/1‑136 (MQ=255) aCCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGCCGTCGCGCGCCCCG‑CCCTCCCACCAGCAACGCGGCGAGAATCCATTATCATTTCTGCCGCTACCGACTa > 4:272450/1‑136 (MQ=255) tCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGCCGTCCCGCGCCCGG‑CCCGCCCACAAGCAACGCTGCGAGAATCCATGATAATTTCTGCCTCTACCGACTATCGcgcc > 7:100421/1‑136 (MQ=255) aGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGCCGTCGCGCGCCCGG‑CCCTCCCCCCACCAACGCTGCGAGCAACCATGATCATTTCTGCCTCGACCGCCGAACGcgccccc > 4:43588/1‑133 (MQ=255) aGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGCCGTCGCGCGCCCGG‑CACTCCCACAACCAACGCTGCGAGAATCCATGATCCTTTCTGCCTCTACCGACTAACGCGCcccg > 7:186264/1‑136 (MQ=255) ttACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGGCCAGTAGCGGGCCCCG‑CCCTGCCACAAGCAACCGGTCGAGAATGCATGATGATTTCTGCCGCTACCTCCTATACCCCCGCCGccc > 4:223490/1‑136 (MQ=255) tACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGCCGTCGCGCGCCCGG‑CCCTCCCCCCAGCAACGCTGCGAGCATCCATGCTCATTTCTGCCTCTACCGACTATCGCGCCGCGGcc > 2:194737/1‑134 (MQ=255) ggCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGCCGTCGCGCGCCCGG‑CACTCCCCCAAGCCACGCAGCGAGAATCCCTGATAATTTCTGCCGCTAGCGACTATCGCGCCGCCGCCCCACga > 8:157176/1‑135 (MQ=255) ccGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGCCGTCGCGCGCCCCG‑CCCGCCCACCAGCAACGCAGCGCGCATCCAAGCTCCTTTCTGCCTCTTACGCCTATCGCGCCCCGGCCCCACCCCAGcag > 8:245251/1‑134 (MQ=255) ccGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGCCGGCGCGCGCCCCG‑CCCTGCCACAACGCACGCTGCGGGGAAGCAGGTACATTTGTGGCACTACCGGCTATCGCGCCGCGGGCCCACGCCAGcgg > 6:56837/1‑134 (MQ=255) ttCATCACTAAAGTGAAAGGGGCCGGGCGCCGTCGCGCGCCCGG‑CCCTCCCACAAGCCACGCAGCGCGAATCCATGATCATTTCTTCCTCTACCGACTATCGCGCCGCGGCCCCACGCCAGCTGCCCCCCGTCCTg > 3:164207/1‑136 (MQ=255) cggggGCGGGCCGCGGCGCGCGCCCGGCCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCAAGCTGCCCCCCTTCCTGTTCCACTACGCTGAc < 2:240920/134‑1 (MQ=255) ggccgcccCCGGCCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCAAGCTGCCCCCCTTCCTGTTCCACTACGCTGACGGCGGCGCCTACGCCg < 4:330219/133‑1 (MQ=255) gcggccgcCCGGCCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCGCTACCGAGAATCGCGCCGCGGCCCAACGCAAGCTGCCCCCCTTCCTGTTCCACTACGCTGACGGCGGCGCCTACGCCg < 5:56837/131‑1 (MQ=255) ccGG‑CACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCAAGCTGCCCCCCTTCCTGTTCCACTACGCTGACGGCGGCGCCTACGCCGa > 4:19584/1‑128 (MQ=255) ccGG‑CACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCAAGCTGCCCCCCTTCCTGTTCCACTACGCTGACGGCGGCGCCTACGCCGa < 3:19584/128‑1 (MQ=255) gcccACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCAAGCTGCCCCCCTTCCTGTTCCACTACGCTGACGGCGGCGCCTACGCCGAGCACACCCt < 3:43588/133‑1 (MQ=255) | ACCGTCAAGCACAGCCTGTTCTTCGCCACCATCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGCCGTCGCGCGCCCGG‑CACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCAAGCTGCCCCCCTTCCTGTTCCACTACGCTGACGGCGGCGCCTACGCCGAGCACACCCT > ACB122/5379083‑5379341 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |