Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	ACB122	1,543,310	Δ3 bp	5.3%	coding (654‑656/1641 nt)	groL →	60 kDa chaperonin

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	ACB122	1543313 =	60 (0.910)	3 (0.050)	3/214	NT	5.3%	coding (657/1641 nt)	groL	60 kDa chaperonin
	?	ACB122	= 1543309	53 (0.870)					coding (653/1641 nt)	groL	60 kDa chaperonin

CTACCAGGGTAGCCAGCGCTTCACCTTCAACGTCTTCGGCAACGATCAGCAGTGGGCGGCCGGCCTTGGCAACGGCTTCCAGAACTGGCAGCAGCTCACGGATGTTGGAGATTTTCTTGTCGACCAGCAGCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  ACB122/1543446‑1543313 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cagcagcagcGGGCCTTCCAGCTCGGCAACCATGGTGTCCGGCTTGTTGACGAAGTACGGCGACAGGTAGCCGCGGTCGAACTGCATGCCTTCTACGACGGACAGTTCGTTTTCCAGGCCCGAGCCTTCTTCAACGGTGATCA  <  ACB122/1543309‑1543177                                                                                                                                                                                                                                                                             CTACCAGGGTAGCCAGCGCTTCACCTTCAACGTCTTCGGCAACGATCAGCAGTGGGCGGCCGGCCTTGGCAACGGCGTCCAGAACTGGCAGCAGCTCACGGATGTTGGAGATTTTCTTGTCGACCAGCAGCAtca                                                                                                                                      <  6:178264/135‑4                                      GGCAACGATCAGCGGTGGGCGGCCGGCCTTGGCAACGGCTTCCAGAACTGGCAGCAGCTCACGGATGTTGGAGATTTTCTTGTCGACCAGCAGCAGCGGGCCTTCCAGCTCGGCAACCATGGTGTCCGGCTTGTT                                                                                                 >  6:26609/1‑135                                                                           GCTTCCAGAACTGGCAGCAGCTCACGGATGTTGGAGATTTTCTTGTCGACCAGCAGCAGCGGGCCTTCCAGCTCGGCAACCATGGTGTCCGGCTTGTTGACGAAGTACGGCGACAGGTAGCCGCGGTCGAACTGC                                                            <  5:26609/135‑1                                                                                                               ATTTTCTTGTCGACCAGCAGCAGCGGGCCTTCCAGCTCGGCAACCATGGTGTCCGGCTTGTTGACGAAGTACGGCGACAGGTAGCCGCGGTCGAACTGCATGCCTTCTACGACGGACAGTTCGTTTTCCAGGCCC                        <  4:128156/135‑1                                                                                                                             CAGCAGCAGCGGGCCTTCCAGCTCGGCAACCATGGTGTCCGGCTTGTTGACGAAGTACGGCGACAGGTAGCCGCGGTCGAACTGCATGCCTTCTACGACGGACAGTTCGTTTTCCAGGCCCGAGCCTTCTTCAAC          >  8:78164/1‑135                                                                                                                                CAGCAGCGGGCCTTCCAGCTCGGCAACCATGGTGTCCGGCTTGTTGACGAAGTACGGCGACAGGTAGCCGCGGTCGAACTGCATGCCTTCTACGACGGACAGTTCGTTTTCCAGGCCCGAGCCTTCTTCAACGGT       <  5:159384/135‑1                                                                                                                                  GCAGCGGGCCTTCCAGCTCGGCAACCATGGTGTCCGGCTTGTTGACGAAGTACGGCGACAGGTAGCCGCGGTCGAACTGCATGCCTTCTACGACGGACAGTTCGTTTTCCAGGCCCGAGCCTTCTTCAACGGTGA     >  8:50852/1‑135                                                                                                                                   CAGCGGGCCTTCCAGCTCGTCAACCATGGTGTACTGCTTGTTGACGAAGTACGGCGACAGGTAGCCGCGGTCGAACTTCATGCCTTCTACGACGGACTTTTCGTTTTCCAGGCCCGAGCCTTCTTCAACGGTGAT    <  7:412330/135‑1                                                                                                                                     GCGGGCCTTCCAGCTCGGCAACCATGGTGTCCGGCTTGTTGACGAAGTACGGCGACAGGTAGCCGCGGTCGAACTGCATGCCTTCTACGACGGACAGTTCGTTTTCCAGGCCCGAGCCTTCTTCAACGGTGATCA  <  2:9156/135‑1                                                                                                                                     GCGGGCCTTCCAGCTCGGCAACCATGGTGTCCGGCTTGTTGACGAAGTACGGCGACAGGTAGCCGCGGTCGAACTGCATGCCTTCTACGACGGACAGTTCGTTTTCCAGGCCCGAGCCTTCTTCAACGGTGATCA  <  3:121081/135‑1                                                                                                                                                                                                                                                                             CTACCAGGGTAGCCAGCGCTTCACCTTCAACGTCTTCGGCAACGATCAGCAGTGGGCGGCCGGCCTTGGCAACGGCTTCCAGAACTGGCAGCAGCTCACGGATGTTGGAGATTTTCTTGTCGACCAGCAGCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  ACB122/1543446‑1543313 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cagcagcagcGGGCCTTCCAGCTCGGCAACCATGGTGTCCGGCTTGTTGACGAAGTACGGCGACAGGTAGCCGCGGTCGAACTGCATGCCTTCTACGACGGACAGTTCGTTTTCCAGGCCCGAGCCTTCTTCAACGGTGATCA  <  ACB122/1543309‑1543177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.