Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 4,245,134 A→C 27.9% L182V (TTG→GTG)  CJ019_03982 ← Crotonyl‑CoA hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1224,245,1340AC27.9% 56.9 / 4.3 52L182V (TTG→GTG) CJ019_03982Crotonyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  ref base A (21/14);  new base C (3/13);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 7.61e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGGCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGT  >  ACB122/4245010‑4245265
                                                                                                                            |                                                                                                                                   
cGGGCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACGTCGCTGACCAACCCGGCGGcc                                                                                                                           >  8:174169/1‑135 (MQ=255)
      ccGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCCGGCGCCCGGCCCGTTCGGCGGCAACGGCAGGCCACTGCTCGTCGGCGCAGACTTCGCTGACCACCCCGGCGGccccgggg                                                                                                                     >  5:172473/1‑129 (MQ=255)
          cgcgTATCCGCCGCTTGGTCTGCGCAACGGCTAAGGTCGGGCCATGGGCCAGGCGCCCGGCCAGTTCGGCGGAAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGAt                                                                                                                 <  2:240001/135‑1 (MQ=255)
          cgcgTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGAt                                                                                                                 <  7:419585/135‑1 (MQ=255)
          cgcgTAGCAGCCGCTTGGTCGGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCGCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGAt                                                                                                                 <  4:441438/135‑1 (MQ=255)
                    ccGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCca                                                                                                       <  7:373889/135‑1 (MQ=255)
                          ggTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAgg                                                                                                                        >  6:19431/1‑112 (MQ=255)
                          ggTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAgg                                                                                                                        <  5:19431/112‑1 (MQ=255)
                            tCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACtcg                                                                                                <  3:176702/134‑1 (MQ=255)
                                cgcATCGGCGATGGGCGGGCCATTGGCCAGGCGCCCGGCCAGGTGGGGGGCAACGGCAGGAAACTGGTGGTCGGCGCATACTGCGCGCACCAACCGGGCGGCGAGGGCGCGATGGGCGCCCCACAACTCGTCGAg                                                                                           <  4:228067/135‑1 (MQ=255)
                                 gcATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCCACCCGGCGGCCCGGGCGCGCTCGGCGCCCCCCCACTCGGCGAg                                                                                           >  4:88113/1‑134 (MQ=255)
                                   aTAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCCCAACTCGGCGAGGaa                                                                                        >  2:364660/1‑135 (MQ=255)
                                     aGGCGATGGTGGGGCCTTTGGCCTGGCGCCCGGCCAGTTGGGCGGCAACGGCTGGCAACTGCTCGTGGGCGCATACTTCGCTGACCAACCCGGGGGCCTGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAAca                                                                                      <  3:217168/135‑1 (MQ=255)
                                             gTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCACCACTCCTCGCCGAACACCCGgcgc                                                                              >  7:130940/1‑135 (MQ=255)
                                             gTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCCCCACACGTCGAGGAACAGCCGgcgc                                                                              >  3:185795/1‑135 (MQ=255)
                                               ggggCCATGGCCCAGGCGCCCGGCCAGTGCGGCGCCAACGCCAGGCAACTGCGCGGCGGCGCAGACTTCGCTCACCACCCGGGCGGCCTGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCtt                                                                            <  3:139899/134‑1 (MQ=255)
                                                    cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGCCCACCCCGGCGGCCCGGGCGCGCTCGGCGCCCCCCCACGCGTCGCGGGACCGCCGGCGCGTgggcg                                                                       >  7:205758/1‑132 (MQ=255)
                                                    cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCCCCCCGGCGGCCCGGGCGCGCGGGGGGGCCCCCCACGCGTCGGGGGACCGCCGGCGCGTgggcg                                                                       >  5:314110/1‑132 (MQ=255)
                                                    cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGGGCGCTCGGGGCCCCCCCACCCGGGGGGGGACACCCGGCGCTgggggt                                                                       >  1:211631/1‑132 (MQ=255)
                                                    cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCCCAACTCGTCGAGGAACAGCAGGCGCTTGGCCt                                                                       >  7:143290/1‑135 (MQ=255)
                                                    cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCCCTCGGGGCCCCCCCACACGTCGAGGCACCCCACGCCCGTGGCCt                                                                       >  7:133483/1‑135 (MQ=255)
                                                    cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCt                                                                       >  8:348564/1‑135 (MQ=255)
                                                    cATTGGCCAGGCGCCCGGCCAGTTAGGCGGCAACGGCAGGCAACAGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGCCGCCCCACAACTCCTCGAGGAACACCAGGCGCATGGcca                                                                       >  3:134025/1‑134 (MQ=255)
                                                       gggCCTGGCGCCCGGCCAGTGCGGCGGCAACGGCTGGCAACTGGTGGTGGGGGCTGACTTGGCTGACCACCCGGGCGGCATGGGCGGGATCGGGGCCCCACAACTCGGCGAGGAACAGCAGGCGCTTGGCCTGCt                                                                    <  7:136865/134‑1 (MQ=255)
                                                          ccAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATAGGCGCCCCCCCACACGGCGGGGCACACCACGCGCCTGGCCGGcgggg                                                                 >  4:139899/1‑131 (MQ=255)
                                                              gcgcCGGGCCTGTGCGGCGGCAACGGCAGGCACCTGCGCGGCGGCGCAGACTTCGCTCACCACCCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGcc                                                             <  4:284050/135‑1 (MQ=255)
                                                               cgcCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGGCCCCCCCCACGTCGCGGGACACCCGGCCCGTGGGCGGCTCGCGGCCg                                                            >  6:278816/1‑135 (MQ=255)
                                                                      ccAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCCCAACTCGGCGAGGCACAGCACGCGCGTTGCCTGCTCGCTGCCGCTGAGcc                                                     >  8:137439/1‑135 (MQ=255)
                                                                        tgTTGGGCGGCAACGGCAGGCACCGGCGCGGCGGCGCAGACTTCGCTCACCACCCCGGCGCCCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGt                                                   <  3:457201/134‑1 (MQ=255)
                                                                                  cAACGGCAGGCACCTGCGCGGCGGCGCAGACTTCGCTCACCAACCCGGCGGCCAGGGCGCGATGGCCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGcc                                         <  7:290092/135‑1 (MQ=255)
                                                                                       gcaggcaACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCCGGGCCCCCCCACACGTCGGGGGACAGCCCGCGCTTGGCCGGCTAGCTGCCGATCAGCCCGGGCCGGTGGCAGcgg                                    >  2:243948/1‑133 (MQ=255)
                                                                                              acggcgcgtcgGCGCAGCCTGCGCGGACCCTCCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGCGGAACAGCAGGCGCTTGTCCTGCTCGCTGCCGAGCAGCCGTGGCAGGTGCCAGCTGGCGCCgg                             <  8:351151/129‑1 (MQ=255)
                                                                                              aCTGCGCGTCGGCGCAGACTTCGCCGACCACCCCGGCGGCCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCgg                             <  8:296343/135‑1 (MQ=255)
                                                                                                tGCTCGGCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                           <  2:10060/135‑1 (MQ=255)
                                                                                                ggcgcgtcgGCGCAGACTTCGCTGACCACCCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                           <  2:341540/131‑1 (MQ=255)
                                                                                                ggCTCGGCGGCGCAGCCTGCGCGCCCCACCCCGGCGCCCCGGGCGCGAGCGCCCCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                           <  5:113319/134‑1 (MQ=255)
                                                                                                     gtcgGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGCCCCCCCACACGGCGAGGCACAGCAGGCGCGTGGCCTGCTCGCTGCCGCTCCGCCGGGGCAGGTGCCCGCTGGCGCCGGCCTCggg                      >  3:134774/1‑135 (MQ=255)
                                                                                                     gtcgGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCCCAACACGGCGCGGCACAGCAGGCGCGTGGGCGGCTCGGTGCCGCACACCCGGGGCAGGTGCCCGCTGGCGCCGGCCTCggg                      >  6:436231/1‑135 (MQ=255)
                                                                                                      tcgGCGCAGACTTCGCTGACCACCCCGGCGGCCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCgggg                     <  8:133483/135‑1 (MQ=255)
                                                                                                      gcgGCGCAGACTTCGCCCACCACCCGGGCGGCCTGGGCTGGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCGCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCgggg                     <  3:37744/134‑1 (MQ=255)
                                                                                                      gcgGCGAAGACTTCGCTGACCGACCCGGCGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCgggg                     <  4:27086/134‑1 (MQ=255)
                                                                                                      ccgGGGCAGACTTCGCGAACAACCCCGGCGGCCAGGGCGGGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCggg                      <  1:53617/133‑1 (MQ=255)
                                                                                                      caggCGCACACTTCGCTGACCACCCCGGCGCCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCgggg                     <  8:136828/133‑1 (MQ=255)
                                                                                                       cgGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGGTCGGCGCCCCCCAACTCGTGGAGGTACAGCAGGGGCGTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCCGCTGGCGCCGGCCTCGGGGc                    >  5:85025/1‑135 (MQ=255)
                                                                                                           gcAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTa                <  7:361861/135‑1 (MQ=255)
                                                                                                                    gctccccACCCCGGCGGCCTGGGCGCGATCGGCGCCCCACAACTCGTCGAGTAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGc        <  3:207728/129‑1 (MQ=255)
                                                                                                                      tGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACCACTCGTCGAGGAACAGCAGGCGCGTGGCCTGCTCGCGGCCGAGAAGCCGTGGCAGGTGCCCGCGGGCGCCGGCATCCGGGCTGTAGCCCATGCTgg     >  4:200779/1‑135 (MQ=255)
                                                                                                                       gACCAACCCGGCGGCCCGGGCGCGATAGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGCTCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgt    >  8:116117/1‑135 (MQ=255)
                                                                                                                       gACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCtctg    >  7:136828/1‑132 (MQ=255)
                                                                                                                         ccACCCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  1:254260/135‑1 (MQ=255)
                                                                                                                         ccAACCGGGCGGCCAGGGCGCTATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  3:177055/135‑1 (MQ=255)
                                                                                                                         ccAACCCGGCGGCCCGGGCGCTATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  7:123549/135‑1 (MQ=255)
                                                                                                                            |                                                                                                                                   
CGGGCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGT  >  ACB122/4245010‑4245265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑