Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 4,350,051 A→G 6.5% L26S (TTG→TCG)  tcrX ← putative transcriptional regulatory proteinTcrX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1224,350,0510AG 6.5% 193.3 / 2.2 61L26S (TTG→TCG) tcrXputative transcriptional regulatory proteinTcrX
Reads supporting (aligned to +/- strand):  ref base A (24/33);  new base G (2/2);  total (26/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGCGCCAACTCCTCACCGTTGAGTAGCGGCATCATGTAGTCGGTAATCACCAGCTGCACGCGTTTTTCCTTCAACGCCTCCAGTGCTTTGCAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCAGGATGGTGTTCATCGGCTCGTCCGTCACCCGCTCTCCTGCGGCGTCGGCGTTCCCGAAAGCACACCGCAGGCACCTTCGAAC  >  ACB122/4349918‑4350181
                                                                                                                                     |                                                                                                                                  
aCGCGCCAACTCCTCACCGTTGAGTAGCGGCATCATGTAGTCGGTAATCACCAGCTGCACGCGTTTTTCCTTCAACGCCTCCAGTGCTTTGCAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCaa                                                                                                                                   >  8:124756/1‑135 (MQ=255)
      cAACTCCTCACCGTTGAGTAGCGGCATCATGTAGTCGGTAATCACCAGCTGCACGCGTTTTTCCTTCAACGCCTCCAGTGCTTTGCAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCaaa                                                                                                                             <  1:183470/135‑1 (MQ=255)
                ccGTTGAGTAGCGGCATCATGTAGTCGGTAATCACCAGCTGCACGCGTTTTTCCTTCAACGCCTCCAGTGCTTTGCAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGa                                                                                                                     <  4:338512/133‑1 (MQ=255)
                 cGTTGAGTAGCGGCATCATGTAGTCGGTAATCACCAGCTGCACGCGTTTTTCCTTCAACGCCTCCAGTGCTTTGCAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGatat                                                                                                                  >  5:321432/1‑135 (MQ=255)
                   ttGAGTAGCGGCATCATGTAGTCTGTAATCACCAGCTGCACGCGTTTTTCCTTCAACGCCTCCAGTGCTTTGCAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCACGGCAAACCCGAGGATATCg                                                                                                                <  8:130306/135‑1 (MQ=255)
                      aGTAGCGGCATCATGTAGTCGGTAATCACCAGCTGCACGCGTTTTTCCTTCAACGCCTCCAGTGCTTTGCAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGc                                                                                                              <  1:217441/134‑1 (MQ=255)
                      aGTAGCGGCATCATGTAGTCGGTAATCACCAGCTGCACGCGTTTTTCCTTCAACGCCTCCAGTGCTTTGCAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCg                                                                                                             >  8:448785/1‑135 (MQ=255)
                       gTAGCGGCATCATGTAGTCGGTATTCACCAGGTGCACGCGTTTTTCCTTCAACGACTCCAGTGCTTTGCAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCAAAGGCAAACCCGAGGATATCGGCGa                                                                                                            >  6:378872/1‑135 (MQ=255)
                       gTAGCGGCATCATGTAGTCGGTAATCACCAGCTGCACGCGTTTTTCCTTCAACGCCTCCAGTGCTTTGCAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGa                                                                                                            >  7:290057/1‑135 (MQ=255)
                            ggCATGATGGAGTCGGTAATCACCAGCTGCACGCGTTTTTCCTTGAACGCCTCCAGTGCTTTGCAGCCATTGCTCGCCTTTTCCAGCAGGAAACGTTCGTCCTCCAATGCAAACCCGAGGATATCGGCGATCAgg                                                                                                       <  7:449095/135‑1 (MQ=255)
                                   tGTAGTCTGTAATCACCAGCTGCACGCGTTTTTCCTTCAACGCCTCCAGTGCTTTGCAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGt                                                                                                <  8:11655/135‑1 (MQ=255)
                                   tGTAGTCGGTAATCACCAGCTGCAGGCGTTTTTCCTTCAACGCCTCCAGTGCTTTGCAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGt                                                                                                <  1:339059/135‑1 (MQ=255)
                                   tGTAGTCGGTAATCACCAGCTGCACGCGTTTTTCCTTCAACGCCTCCAGTGCTTTGCAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCGAGGCAAACCCGAGGATATCGGCGATCAGGTATTCg                                                                                                 >  3:325386/1‑134 (MQ=255)
                                   tGTAGTCGGTAATCACCAGCTGCACGCGTTTTTCCTTCAACGCCTCCAGTGCTTTGCAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCGAGGCAAACCCGAGGATATCGGCGATCAGGTATTCg                                                                                                 >  3:319437/1‑134 (MQ=255)
                                   tGTAGTCGGTAATCACCAGCTGCACGCGTTTTTCCTTCAACGCCTCCAGTGCTTTGCAGCCATTGCTCGCCTTTTCCACAAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGATAAGGTATTCg                                                                                                 >  6:39363/1‑134 (MQ=255)
                                          tgTAATCACCAGCTGCACGCGTTTTTCCTTCAACGCCTCCAGTGCTTTGCAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGAc                                                                                         <  7:339936/134‑1 (MQ=255)
                                           gTAATCACCAGCTGCACGCGTTTTTCCTTCAACGCCTCCAGTGCTTTGCAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGAc                                                                                         <  2:337054/134‑1 (MQ=255)
                                           gTAATCACCAGCTGCACGCGTTTTTCCTTCAACGCCTCCAGTGCTTTGCAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACa                                                                                        >  8:7246/1‑135 (MQ=255)
                                           gTAATCACCAGCTGCACGCGTTTTTCCTTCAACGCCTCCAGTGCTTTGCAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACa                                                                                        >  3:214985/1‑135 (MQ=255)
                                               tCACCAGCTGCACGCGTTTTTCCTTCAACGCCTCCAGTGCTTTGCAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCa                                                                                    >  3:17727/1‑135 (MQ=255)
                                               tCACCAGCTGCACGCGTTTTTCCTTCAACGCCTCCAGTGCTTTGCAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCa                                                                                    <  5:143544/135‑1 (MQ=255)
                                               tCACCAGCTGCACGCGTTTTTCCTTCAACGCCTCCAGTGCTTTGCAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCa                                                                                    <  7:448785/135‑1 (MQ=255)
                                               tCACCAGCTGCACGCGTTTTTCCTTCAACGCCTCCAGTGCTTTGCAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCa                                                                                    <  8:246864/135‑1 (MQ=255)
                                                     gCTGCACGCGTTTTTCCTTCAACGCCTCCAGTGCTGTGCAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCAGGATgg                                                                              >  3:415536/1‑135 (MQ=255)
                                                         cACGCGTTTTTCCTTCAACGCCTCCAGTGCTTTGCAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCGAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCAGGATGGTGtt                                                                          <  4:319437/135‑1 (MQ=255)
                                                         cACGCGTTTTTCCTTCAACGCCTCCAGTGCTTTGCAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCAGGATGgtgt                                                                           >  3:147266/1‑134 (MQ=255)
                                                          aCGCGTTTTTCCTTCAACGCCTCCAGTGCTTTGCAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCGAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCAGGATGGTGtt                                                                          <  4:325386/134‑1 (MQ=255)
                                                          aCGCGTTTTTCCTTCAACGCCTCCAGTGCTTTGCAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCAGGATGGTGtt                                                                          <  4:160829/134‑1 (MQ=255)
                                                          aCGCGTTTTTCCTTCAACGCCTCCAGTGCTTTGCAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCAGGATGGTGtt                                                                          <  4:135134/134‑1 (MQ=255)
                                                            gcgTTTTTCCTTCACCGCCTCCAGTGCTTTGCAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCAGGATGGTGTTCAt                                                                       >  6:434293/1‑135 (MQ=255)
                                                            gcgTTTTTCCTTCAACGCCTCCAGTGCTTTGCAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCAGGATGGTGTTCAt                                                                       >  7:142912/1‑135 (MQ=255)
                                                              gttgttCCTTCAACGCCTCCAGTGCTTTGCAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCAGGATGGTGTTCATCg                                                                     <  5:175050/135‑1 (MQ=255)
                                                              gTTTTTCCTTCAACGCCTCCAGTGCTTTGCAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCAGGATGGTGTTCATCg                                                                     >  1:459004/1‑135 (MQ=255)
                                                              gTTTTTCCTTCAACGCCTCCAGTGCTTTGCAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCAGGATGGTGTTCATCg                                                                     <  6:205965/135‑1 (MQ=255)
                                                                           cGCCTCCAGTGCTTTGCAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCAGGATGGTGTTCATCGGCTCGTCCGTCAc                                                        <  8:341697/135‑1 (MQ=255)
                                                                           cGCCTCCAGTGCTTTGCAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCAGGATGGTGTTCATCGGCTCGTCCGTCAc                                                        >  6:450762/1‑135 (MQ=255)
                                                                                  aGTGCTTTGCAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCAGGATGGTGTTCATCGGCTCGTCCGTTACCCGctct                                                 <  1:158368/135‑1 (MQ=255)
                                                                                  aGTGCTTTGCAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCAGGATGGTGTTCATCGGCTCGTCCGTTACCCGctct                                                 <  1:164481/135‑1 (MQ=255)
                                                                                          gCAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCAGGATGGTGTTCATCGGCTCGTCCGTCACCCGCTCTCCTgcgg                                          <  4:24935/134‑1 (MQ=255)
                                                                                           cAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCAGGATGGTGTTCATCGGCTCGTCCGTCACCCGCTCTCCTgcggcg                                        >  6:232921/1‑135 (MQ=255)
                                                                                            aGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCAGGATGGTGTTCATCGGCTCGTCCGTCACCCGCTCTCCTGcggcgt                                       >  4:343582/1‑135 (MQ=255)
                                                                                            aGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCAGGATGGTGTTCATCGGCTCGTCCGTCACCCGCTCTCCTGcggcgt                                       >  2:89576/1‑135 (MQ=255)
                                                                                              ccATTGATCGCCTGTTCCACCAGGAAACCTTCGTCCTCCAACGCAAACCCGACGATATCGGCGATCAGGTATTCGTCATCGACAATCAGGATGGTGTTCATCGGCTCGTCCGTCACCCGCTCTCCTGcggcgtcg                                     <  2:445175/135‑1 (MQ=255)
                                                                                                aTTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCAGGATGGTGTTCATCGGCTCGTCCGTCACCCGCTCTCCTGcggcgtcggc                                   <  3:134256/135‑1 (MQ=255)
                                                                                                  tGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCAGGATGGTGTTCATCGGCTCGTCCGTCACCCGCTCTCCTGcggcgtcggcgt                                 <  4:214985/135‑1 (MQ=255)
                                                                                                   gCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCAGGATGGTGTTCATCGGCTCGTCCGTCACCCGCTCTCCTGCGGCGTCGGCGtt                                >  7:267570/1‑135 (MQ=255)
                                                                                                    cTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCAGGATGGTGTTCATCGGCTCGTCCGTCACCCGCTCTCCTGCGGCGTCGGCGTTc                               >  2:74435/1‑135 (MQ=255)
                                                                                                      cGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCAGGATGGTGTTCATCGGCTCGTCCGTCACCCGCTCTCCTGCGGCGTCGGCGTTcc                              >  6:366767/1‑134 (MQ=255)
                                                                                                          ttttCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCAGGATGGTGTTCATCGGCTCGTCCGTCACCCGCTCTCCTGCGGCGTCGGCGTTCCCGaaa                         <  8:44806/135‑1 (MQ=255)
                                                                                                                     gAAACCTTCGTCCTCCAATGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCAGGATGGTGTTCATCGGCTCGTCCTTCACCCGCTCTCCTGCGGCGTCGGCGTTCCCGAAAGCACTCCGCAg              <  8:71720/135‑1 (MQ=255)
                                                                                                                      aaaCCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGAc                                                                                         >  7:252116/1‑59 (MQ=255)
                                                                                                                      aaaCCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGAc                                                                                         <  8:252116/59‑1 (MQ=255)
                                                                                                                      aaaCCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCAGGATGGTGTTCATCGGCTCGTCCGTCACCCGCTCTCCTGCGGCGTCGGCGTTCCCGAAAGCACACCGCAgg             >  2:306893/1‑135 (MQ=255)
                                                                                                                              gTCCTCCAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCAGGATGGTGTTCATCGGCTCGTCCGTCACCCGCTCTCCTGCGGCGTCGGCGTTCCCGAAAGCACACCGCAGGCTCCTTc      <  2:376789/134‑1 (MQ=255)
                                                                                                                               tcctccAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCa                                                                                    <  5:209289/55‑1 (MQ=255)
                                                                                                                               tcctccAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCa                                                                                    >  6:209289/1‑55 (MQ=255)
                                                                                                                                cctccAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCAGGATGGTGTTCATCGGCTCGTCCGTCACCCGCTCTCCTGCGGCGTCGGCGTTCCCGAAAGCACACCGCAGGCACCTTCGaa   >  8:433944/1‑135 (MQ=255)
                                                                                                                                 ctccAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCAGGATGGTGTTCATCGGCTCGTCCGTCACCCGCTCTCCTGCGGCGTCGGCGTTCCCGAAAGCACACCGCAGGCACCTTCGAAc  <  6:25269/135‑1 (MQ=255)
                                                                                                                                 ctccAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCAGGATGGTGTTCATCGGCTCGTCCGTCACCCGCTCTCCTGCGGCGTCGGCGTTCCCGAAAGCACACCGCAGGCACCTTCGAAc  <  4:17727/135‑1 (MQ=255)
                                                                                                                                 ctccAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCAGGATGGTGTTCATCGGCTCGTCCGTCACCCGCTCTCCTGCGGCGTCGGCGTTCCCGAAAGCACACCGCAGGCACCTTCGAAc  <  5:366767/135‑1 (MQ=255)
                                                                                                                                 ctccAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCAGGATGGTGTTCATCGACTCGTCCGTCACCCGCTCTCCTGCGGCGTCGGCGTTCCCGAAAGCACACCGCAGGCACCTTCGAAc  <  5:257668/135‑1 (MQ=255)
                                                                                                                                  tccAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCAGGATGGTGTTCATCGGCGCGTCCGTCACCCGCTCTCCTGCGGCGTCGGCGTTCCCGAAAGCACACCGCAGGCACCTTCGAAc  <  1:110752/134‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                  
ACGCGCCAACTCCTCACCGTTGAGTAGCGGCATCATGTAGTCGGTAATCACCAGCTGCACGCGTTTTTCCTTCAACGCCTCCAGTGCTTTGCAGCCATTGCTCGCCTTTTCCACCAGGAAACCTTCGTCCTCCAAGGCAAACCCGAGGATATCGGCGATCAGGTATTCGTCATCGACAATCAGGATGGTGTTCATCGGCTCGTCCGTCACCCGCTCTCCTGCGGCGTCGGCGTTCCCGAAAGCACACCGCAGGCACCTTCGAAC  >  ACB122/4349918‑4350181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑