| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 5,262,927 | T→G | 54.0% | intergenic (+27/+9) | uacT_2 → / ← radA | Uric acid transporter UacT/DNA repair protein RadA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 5,262,927 | 0 | T | G | 54.0% | ‑4.8 / 3.3 | 18 | intergenic (+27/+9) | uacT_2/radA | Uric acid transporter UacT/DNA repair protein RadA |
| Reads supporting (aligned to +/- strand): ref base T (4/3); new base G (7/2); total (11/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01 | |||||||||||
CAAGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTGGGCACGATGGCACGCTTGAAGCCGTGCTTGGCC > ACB122/5262795‑5263059 | cAAGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCACGCGCGAGCTTTCTCGCTcc > 7:55433/1‑135 (MQ=255) aaGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCCGGCGCGAGCCTGGTCGCGCCGGCCCGCGAt > 4:407198/1‑135 (MQ=255) aGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGCGCTTGCTCGCTCCAGCCCCCTAtt > 8:154363/1‑135 (MQ=255) tctaggCCGCGGTTAACGATGTGCGGTGGGTGGAACTGCACGACCACGGAAATCAACCGGCCCGGCCCCCGGCGGTTGCCGAGCACTGAGGGGTGGCTGGCGCGAGCTTGCTCGCGCCTGCACGCTATTCGAACa < 8:55433/130‑1 (MQ=255) gATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGCGCTTGCTCGCGCCTGGACGCTATTAGAACaaa > 3:444261/1‑134 (MQ=255) cGAGGGTAACGAAATGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGCGCGCGCTTGCTCGCGCCGGGCCGCGATTAGCACAAttcctc > 2:364683/1‑130 (MQ=255) aGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCCGGAGCGAGCTTGCTCGCGCCTGCACGCGATTCGAACAAAGCaaacc > 8:147445/1‑131 (MQ=255) aaCGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCCCGCGCGCGCTTGGTCGCTCCTGCCCGCGCTTGGAACAATGCATCCCGCGcc > 3:41939/1‑135 (MQ=255) cTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGCGCGAGCTTTCTGGCGCCGTCACGCGATTAGAACAAAGCCAACCGCCCCGGTTGCAGCCGGGTa > 8:237652/1‑135 (MQ=255) gAGCACGGCAATGAACCGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCCGGAGCGAGCTTGCTCGCGCCTGGACGCGATTCGAACAATGCATCCAGCGCCTGTTGCCGCCCGGTTACGGCCATCa > 4:334002/1‑133 (MQ=255) gAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTTCTCGCTCCTGCACGCTATTCGAACAATGCCTCCAGCGCCTGTTCCAGCCGGGTTACGGCAATCAcc > 4:296919/1‑135 (MQ=255) cGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCACGAGCCCGCTTGCTAGCGCCTGCACGCATTTAGCAAAAAGCATACACCGCCGGTTCCAGCCCGGTAACGGCAATCCCCGGCac > 6:245297/1‑134 (MQ=255) aCGGCGGTGGCCGAGCACTGCCGGGTGGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGgc < 8:348435/135‑1 (MQ=255) cggggTTGCTGGAGCGAGTTTGCTCGCGCGTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTGGGCACGAt < 1:356197/133‑1 (MQ=255) ggggTTGCTGGAGGGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGATTCTTTCGGCGCATTGCCCTTGGGCACGAt < 1:310326/133‑1 (MQ=255) ggggTTGCGGGCGCGCGCTTGCGCGCCCGGGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTGGGCACGATg < 7:154363/134‑1 (MQ=255) ggggTGGCAGCCGCCAGCTGGCGCGCACCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTGGGCACGAt < 6:151489/133‑1 (MQ=255) tGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTGGGCACGATGGCACg < 4:459682/135‑1 (MQ=255) tcgctcCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTGGGCACGATGGCACGCTTGAAGCCGTGCTTgg < 7:342549/135‑1 (MQ=255) gcgcccCGGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTGGGCACGATGGCACGCTTGAAGCCGTGCTTgg < 1:95532/134‑1 (MQ=255) gctcCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTGGGCACGATGGCACGCTTGAAGCCGTGCTTGGcc > 6:373891/1‑135 (MQ=255) ctcCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTGGGCACGATGGCACGCTTGAAGCCGTGCTTGGc < 4:444261/133‑1 (MQ=255) | CAAGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTGGGCACGATGGCACGCTTGAAGCCGTGCTTGGCC > ACB122/5262795‑5263059 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |