Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 2,965,146 T→G 27.0% intergenic (+37/+72) CJ019_02796 → / ← kstD_1 hypothetical protein/3‑oxosteroid 1‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1222,965,1460TG27.0% 88.1 / 3.9 74intergenic (+37/+72)CJ019_02796/kstD_1hypothetical protein/3‑oxosteroid 1‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (27/24);  new base G (7/15);  total (34/40)
Fisher's exact test for biased strand distribution p-value = 1.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCGCTTCCTGCGCACCGAGCAGATCGACGAGGTGGATGTGACCTACTGGCTGGCGCAAATTCAGGAGCGCAGCGCAGCGAGACCTGTGAACTGAGGGGTGACAGCACTGAAAAATCAGACCGCGTGGCGCTCGATTCAAGCGCCACGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGACCACCCGGGTATTGGGGGTCAGCTCACTCATACCCCTGCACCCCGGCAATGTGCCGCCCGGCGACATAGCCAAAGGTCAGCGCCGG  >  ACB122/2965015‑2965280
                                                                                                                                   |                                                                                                                                      
aGCGCTTCCTGCGCACCGAGCAGATCGACGAGGTGGATGTGACCTACTGGCTGGCGCAAATTCAGGAGCGCAGCGCAGCGAGACCTGTGAACTGAGGGGTGACAGCACTGAAAAATCAGACCGCGTGGCGCTCGa                                                                                                                                     >  2:243430/1‑135 (MQ=255)
 gcgcTTCCTGCGCACCGAGCAGATCGACGAGGTGGATGTGACCTACTGGCTGGCGCAAATTCAGGAGCGCAGCGCAGCGAGACCTGTGAACTGAGGGGTGACAGCACTGAAAAATCAGACCGCGTGGCGCTCGAt                                                                                                                                    <  8:151979/135‑1 (MQ=255)
   gcTTCCTGCGCACCGAGCAGATCGACGAGGTGGATGTGACCTACTGGCTGGCGCAAATTCAGGAGCGCAGCGCAGCGAGACCTGTGAACTGAGGGGTGACAGCACTGAAAAATCAGACCGCGTGGCGCTCGAtt                                                                                                                                   >  7:23026/1‑134 (MQ=255)
   gcTTCCTGCGCACCGAGCAGATCGACGAGGTGGATGTGACCTACTGGCTGGCGCAAATTCAGGAGCGCAGCGCAGCGAGACCTGTGAACTGAGGGGTGACAGCACTGAAAAATCAGACCGCGTGGCGCTCGAtt                                                                                                                                   >  4:401849/1‑134 (MQ=255)
        cTGCGCACCGAGCAGATCGACGAGGTGGATGTGACCTACTGGCTGGCGCAAATTCAGGAGCGCAGCGCAGCGAGACCTGTGAACTGAGGGGTGACAGCACTGAAAAATCAGACCGCGTGGCGCTCGCTTCAAgcg                                                                                                                             >  7:128299/1‑135 (MQ=255)
        cTGCGCACCGAGCAGATCGACGAGGTGGATGTGACCTACTGGCTGGCGCAAATTCAGGAGCGCAGCGCAGCGAGACCTGTGAACTGAGGGGTGACAGCACTGAAAAATCAGACCGCGTGGCGCTCGATTCAAgcg                                                                                                                             >  2:13775/1‑135 (MQ=255)
                   gCAGATCGACGAGGTGGATGTGACCTACTGGCTGGCGCAAATTCAGGAGCGCAGCGCAGCGAGACCTGTGAACTGAGGGGTGACAGCACTGAAAAATCAGACCGCGTGGCGCTCGATTCAAGCGCCACGGCCttt                                                                                                                  >  8:58359/1‑135 (MQ=255)
                     aGATCGACGAGGTGGATGTGACCTACTGGCTGGCGCAAATTCAGGAGCGCAGCGCAGCGAGACCTGTGAACTGAGGGGTGACAGCACTGAAAAATCAGACCGCGTGGCGGTCGCTTCCAGCGCCCCGGCCTTTcc                                                                                                                >  6:365610/1‑135 (MQ=255)
                     aGATCGACGAGGTGGATGTGACCTACTGGCTGGCGCAAATTCAGGAGCGCAGCGCAGCGAGACCTGTGAACTGAGGGGTGACAGCACTGAAAAATCAGACCGCGTGGCGCTCGATTCAAGCGCCACGGCCTTTcc                                                                                                                >  4:157028/1‑135 (MQ=255)
                       aTCGCCGAGGTGGATGTGACCTACTGGCTGGCGAAAATTCAGGAGCGCAGCGCAGCGAGACCGGTGACCTACGGGGGGCCAGCACGGAAAAAGCTGACCGCGTGGCGCGCGATCCCCGCGCCACGGCCTTTCCCg                                                                                                              <  4:49213/135‑1 (MQ=255)
                       aTCGACGAGGGGTATGTGACCTACTGGCTGGCGCAAATTCAGGAGCGCAGCGCAGCGAGACCTGTGAACTGAGGGGTGACAGCACTGAAAAATCAGACCGCGGGGCGCTCGATTCACGCGCCACGGCCTTTCCCg                                                                                                              <  4:437209/135‑1 (MQ=255)
                         cgacgaGGGGGATGTGACCTCCTGGCTGGCGCAAATTCAGGAGCGCAGCGCAGCGAGACCTGTGAACTGCGGGGTCACCGCACTGAAAAATCAGACCGCGGGGCGCTCGATTCACGCGCCACGGCCTTTCCCGGc                                                                                                            <  2:26398/135‑1 (MQ=255)
                               ggTGGATGTGACCTACTGGCTGGCGCAAATTCAGGAGCGCAGCGCAGCGAGACCTGTGAACTGAGGGGTGACAGCACTGAAAAATCAGACCGCGTTGCCGGCGCTTCAACCCCCACCGCCGTTACCCGCGtccgg                                                                                                      >  2:433875/1‑133 (MQ=255)
                               ggTGGATGTGACCTACTGGCTGGCGCAAATTCAGGAGCGCAGCGCAGCGAGACCTGTGAACTGAGGGGTGACAGCACTGAAAAATCAGACCGCGTGGCGCTCGATTCAAGCGCCCCGGCCTTTCCCGGCctcctc                                                                                                      >  4:409913/1‑135 (MQ=255)
                               ggTGGATGTGACCTACTGGCTGGCGCAAATTCAGGAGCGCAGCGCAGCGAGACCTGTGAACTGAGGGGTGACAGCACTGAAAAATCAGACCGCGTGGCGCTCGATTCAAGCGCCACGGCCTTTCCCGGCGtcctc                                                                                                      >  4:443522/1‑135 (MQ=255)
                               ggTGGAAGTGACCTACTGGCTGGCGCAAATTCAGGAGCGCAGCGCAGCGAGACCTGGGAACTGAGGGGTGACAGCACTGAAAAATCAGACCGCGTGGCCCTAGATTCAAGCGCCACGGCCTTTCCCGGAGacctc                                                                                                      >  4:394499/1‑135 (MQ=255)
                                 tGGATGTGACCTACTGGCTGGCGCAAATTCAGGAGCGCAGCGCAGCGAGACCTGTGAACTGAGGGGTGACAGCACTGAAAAATCAGACCGCGTGGGGCGCGATTCAAGCGCCACGGCCTTTCCCGGCGTcctcct                                                                                                    >  3:181605/1‑135 (MQ=255)
                                 tGGATGTGACCTACTGGCTGGCGCAAATTCAGGAGCGCAGCGCAGCGAGACCTGTGAACTGAGGGGTGACAGCACTGAAAAAGCAGACCGCGTGGCGCTCGATTCACGCGCCACGGCCTTTCCCGGCGTcctcct                                                                                                    <  8:264170/135‑1 (MQ=255)
                                    aTGTGACCTACTGGCTGGCGCAAATTCAGGAGCGCAGCGCAGCGAGACCTGTGAACTGAGGGGTGACAGCACTGAAAAATCAGACCGCGTGGCGCTCGTTTACAGCGCCACGGCCCTTCCCGGCGTCCTCCTGCa                                                                                                 >  8:251155/1‑135 (MQ=255)
                                    aTGTGACCTACTGGCTGGCGCAAATTCAGGAGCGCAGCGCAGCGAGACCTGTGAACTGAGGGGTGACAGCACTGAAAAATCAGACCGCGTGGCGCTCGAATCAAGCGCCCACGCCTTTCCCGGCGTCCGCCTGCa                                                                                                 >  2:43933/1‑135 (MQ=255)
                                    aTGTGACCTACTGGCGGTCGCAAATTCCGGCGCGCCGCGCAGGGTGACCTGTGAACGGAGGGGGGCCCGCACTGAAAAACCCGCCCGCGGGGCGCTCGTTGCACGCGCCACGGCCTTTCCCGGCGTCCTCCTGCa                                                                                                 <  6:295463/135‑1 (MQ=255)
                                    aTGTCACCTAGGGGCTGGCCCAAATGCAGGCGCGCAGCGCAGCGAGACCTGTTAACTGAGGGGTCACAGCACTGAAAAATCACACCGCGGGGCGCTCGATTCAAGGGCCACGGCCTTTCCCGGCGTCCTCCTGCa                                                                                                 <  6:20827/135‑1 (MQ=255)
                                    aTGTCACATACTGCCTGGCGCAAATTCCGCCGCGCTGCGCTGCTAGACGTGTGAACGGCGGGGTGACAGCACTTAAAAATCCCACCGCGTGGCGCGCGATACACGCGCCACGGCCTTTCCCGGCGTCCTCCTGCa                                                                                                 <  4:214538/135‑1 (MQ=255)
                                         aCCTACTGGCTGGCGCAAATTCAGGAGCGCAGCGCAGCGAGACCTGTGAACTGAGGGGTGACAGCACTGAAAAATCAGACCGCGTGGCGCGCGATTCAAGCGCCACGGCCTTTCCCGGCGTCCGCCTGCAGAgcg                                                                                            >  2:270443/1‑135 (MQ=255)
                                          ccaaCTGGCGGCCGAAAATGCCGCCGCGCAGCGCCGCGAGACCTGTGAACTGGGGGGTGACAGCACTGAAAAACCCCCCCGCGGGGCGCGCGATTCACGCCCCACGGCCTTTCCCGGCGTCCTCCTGCAGAgcgc                                                                                           <  5:117514/132‑1 (MQ=255)
                                            tACTGGCTGGCGCAAATTCAGGAGCGCAGCGCAGCGAGACCTGTGAACTGAGGGGTGACAGCACTGAAAAATCAGACCGCGTTGCGCGCGATTCAAGCGGCCCGGCCTTTACCGGCGGCCGCCGGCAGGGAGccc                                                                                         >  1:396131/1‑134 (MQ=255)
                                            tACTGGCTGGCGCAAATTCAGGAGCGCAGCGCAGCGAGACCTGTGAACTGAGGGGTGACAGCACTGAAAAATCAGACCGCGTGGCGCTCGATTGCAGCGCCCCGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCg                                                                                         >  2:293738/1‑135 (MQ=255)
                                            tACTGGCTGGCGCAAATTCAGGAGCGCAGCGCAGCGAGACCTGTGAACTGAGGGGTGACAGCACTGAAAAATCAGACCGCGTGGCGCTCGATTCCAGCGCCACCGCCTTTCCCCGCGTCCTCCAGCAGAccgccg                                                                                         >  3:57399/1‑135 (MQ=255)
                                            tACTGGCTGGCGCAAATTCAGGAGCGCAGCGCAGCGAGACCTGTGAACTGAGGGGTGACAGCACTGAAAAATCAGACCGCGTGGCGCTCGATTCAAGCGCCACGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCg                                                                                         >  3:71462/1‑135 (MQ=255)
                                              ctggctggCGCAAATTCAGGAGCGCAGCGCAGCGAGACCTGTGAACTGAGGGGTGACAGCACTGAAAAATCAGACCGCGTGGCGCTCGATTCAAGCGCCACGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGac                                                                                       >  4:58330/1‑135 (MQ=255)
                                                ggctggcGCAAATTCAGGAGCGCAGCGCAGCGAGACCTGTGAACTGAGGGGTGACAGCACTGAAAAATCAGACCGCGTGGCGCGCGATTCAAGCGCCCCGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGaccc                                                                                     >  7:16152/1‑134 (MQ=255)
                                                   tggcGCAAATTCAGGAGCGCAGCGCAGCGAGACGTGTGAACTGAGGGGTCACACCACTGAAAAATCAGACCGCGTGGCGCGCTATTCAAGCGCCACGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGACCAccc                                                                                  <  1:13775/135‑1 (MQ=255)
                                                      cgcAATTTCTGTAGCTCAGCGCTGGGAGACGTGTGAAGTTCGGGTTGACAGCACTAAAAAACAAGCCCGCGGGGGGCGCTATTCACGCGCAACGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGACCACCCggg                                                                               <  2:243764/135‑1 (MQ=255)
                                                      cgcAAATTCAGGAGCGCAGCGCAGCGAGACCTGTGAACTGAGGGGTGACAGCACTGAAAAATCAGACCGCGTGGCGCTCGATTCAAGCGCCACGGCCTTTACCGGCGTCCTCCTGCAGAGCGCCGCCCCCCCgg                                                                                >  1:48159/1‑134 (MQ=255)
                                                      cgcAAATTCAGGAGCGCAGCGCAGCGAGACCTGTGAACTGAGGGGTGACAGCACTGAAAAATCAGACCGCGTGGCGCGCGATTCAAGCGCCCCGGCCTTTCCCGGGGTCCTCCTGCAGAGCGCCGACCCCCCcgg                                                                               >  7:236585/1‑135 (MQ=255)
                                                         aaaTTCAGGAGCGCAGCGCAGCGAGACCTGTGAACTGAGGGGTGACAGCACTGAAAAATCAGACCGCGTGGCGCTCGATTCAAGCGCCACGGCCTTTCCCGGCGTCCGCCTGCAGAGCGCCGCCCACCCGGGTAt                                                                            >  3:290208/1‑135 (MQ=255)
                                                           aTTCCGGCGCCCCGCGCCGGGAGACGTGTCAAGTGCGGGGTCACCCCACTAAAAAATCCCCCCGGGGGGCGCGCGATCCACCCCCCACGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGACCACCCGGGTATTg                                                                          <  4:456321/135‑1 (MQ=255)
                                                             tCAGGAGCGCAGCGCAGCGAGACCTGTGAACTGAGGGGTGACAGCACTGAAAAATCAGACCGCGTGGCGCGCGATTCAAGCGCCACGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGACCACCCGGGTATTggg                                                                        <  6:192990/135‑1 (MQ=255)
                                                             cccggAGCGCCGCGCCGCGAGACCTGTGAACTGAGGGGTCACAGCACTTAAAAATCACCCCGCGGGGCGCGCTATTCACGCGCCACGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGACCACCCGGGTATTggg                                                                        <  3:379137/132‑1 (MQ=255)
                                                             cccgccgcgcagcgcaGCGAGACCGGTGAACTCCGGGGTCACCCCACTCAAAAAACACCCCGCGGGGCCCGCGATTCACGCGCCACGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGACCACCCGGGTATTggg                                                                        <  3:416281/129‑1 (MQ=255)
                                                                  cgcgcagcgcaGCGAGCCCGGTGAACTGAGGGGTGACACCACTCAAAAATCAGACCGCGTGGCGCTCGATTCACGCGCCACGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGACCACCCGGGTATTGGGGGTCa                                                                   <  7:32944/134‑1 (MQ=255)
                                                                  cgcgcagcgcaGCGAGACCTGTGAACTGAGGGGTCACACCACTGAAAAAACCCCCCGCGGGGCGCGCGATGCACCCGCCACGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGACCACCCGGGTATTGGGGGTCa                                                                   <  4:157673/134‑1 (MQ=255)
                                                                  agcgcagcgcAGCGAGACCTGTGAACTGAGGGGTGACAGCACTGAAAAATCAGACCGCGTGGCGCTCGATTCAAGCGCCACGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGACCACCCGGGTATTGGGGGTCa                                                                   <  8:257714/135‑1 (MQ=255)
                                                                  agcgaagcgcTGGGAGCCCTGTTAACTGAGGGGTGACAGCACTTAAAAACCAGACCGCGGGGCGCTCGATTCAAGCGCCACGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGACCACCCGGGTATTGGGGGTCa                                                                   <  3:158633/135‑1 (MQ=255)
                                                                  agccccgcgcagctCGACCTGGGAACTGAGGGGTCCCAGCAGTTAAAATCCCGCCCGGGGGCCGCCCGATACAAGCCCCACGGCCTTTCCCGCCGTCCTCCTGCAGAGCGGCGACCACCCGGGTATTGGGGGTCa                                                                   <  4:450271/129‑1 (MQ=255)
                                                                   gcgccgcgcaGCGTGACCTGTCACCTGAGGGGTGACAGAACTGAAAAATCAGCCCGCGTGGCGCGCTATTCAAGCGCCACGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGACCACCCGGGTATTGGGGGTCAg                                                                  <  2:64514/135‑1 (MQ=255)
                                                                   gcgccgcgcaGCGAGACCTGTGAACTGCGGGGTCACACCACTGAAAAATCAGACCGCGTGGCGCGCGATGCACGCGCCACGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGACCACCCGGGTATTGGGGGTCAg                                                                  <  5:95195/135‑1 (MQ=255)
                                                                        gcgcagcgTGACGTGTGACCTGTGGGGGCACAGCACTGAAAAATCAGACCGCGTGGCGCTCTATCCACGCGCCACGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGACCACCCAGGTATTGGGGGTCAGctcac                                                             <  5:206079/135‑1 (MQ=255)
                                                                        gcgcagcgAGACCTGTTAACTTAGGGGTGACAGCACTGAAAACTCAGCCCGCGTGGCTCTCGATGCAAGCGCCACGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGACCACACGGGTATTGGGGGTCAGctcac                                                             <  2:48159/135‑1 (MQ=255)
                                                                        gcgcagcgAGACCTGTGAACTGAGGGGTGACAGCACTTAAAAATCAGACCGCGTGGCGCTCGATTCAAGCGCCACGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGACCACCCGGGTATTGGGGGTCAGctcac                                                             <  6:229212/135‑1 (MQ=255)
                                                                        gcgcagcgAGACCTGTGAACTGAGGGGGGACAGCACTGAAAAATCAGACCGCGTGGCGCGCGATTCAAGCGCCACGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGACCACCCGGGTATTGGGGGTCAGctcac                                                             <  4:228286/135‑1 (MQ=255)
                                                                              ctataCCTGTGAACTTTGGGGTAACAGCATTGATAATACTGACCGGGTGGCGCTGTTTTAACGCGCCAGGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGACCACCCGGGTATTGGGGGTCAGCTCACTCATAc                                                       <  6:386065/131‑1 (MQ=255)
                                                                                agaCCTGTGAACTGAGGGGTGACAGCACTGAAAAATCAGACCGCGTGGCGCTCGATTCAAGCGCCACGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGACCACCCGGGTATTGGGGGTCAGCTCACTCATAccc                                                     <  6:187553/135‑1 (MQ=255)
                                                                                      gtgAACTGAGGGGTGACAGCACTGAAAAATCAGACCGCGTGGCGCTCGATTCAAGCGCCACGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGACCACCCGGGTATTGGGGGTCAGCTCCCTCATACCCCTGCAc                                               >  6:178698/1‑135 (MQ=255)
                                                                                                 ggTGACAGCACTGAAAAATCAGACCGCGTGGCGCTCGATTCAAGCGCCCCGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGACCACCCGGGTATTGGGGGTCAGCTCACTCATACCCCTGCCCCCCGGCAAtgt                                    >  3:36753/1‑135 (MQ=255)
                                                                                                 ggTGACAGCACTGAAAAATCAGACCGCGTGGCGCTCGATTCAAGCGCCACGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGACCACCCGGGTATTGGGGGTCAGCTCACTCATACCCCTGCACCCCGGCAAtgt                                    >  4:229516/1‑135 (MQ=255)
                                                                                                 ggTGACAGCACTGAAAAATCAGACCGCGTGGCGCTCGATTCAAGCGCCACGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGACCACCCGGGTATTGGGGGTCAGCGCACTCATACCCCTGCACCCCGGCAAtgt                                    >  8:158817/1‑135 (MQ=255)
                                                                                                 ggTGACAGCACTGAAAAATCAGACCGCGTGGCGCTCGATTCAAGCGCCACGGCCGTTCCCCGCGGCCTCCAGCACAGCGCCCCCCACCCCGGAATTGGGGGTCAGCTCACTCCAACCCCAGCACCCCCGCAAagt                                    >  4:171469/1‑135 (MQ=255)
                                                                                                 ggTGACAGCACTGAAAAATCAGACCGCGTGGCGCGCGATTCAAGCGCCCCGGCCTTTCCCCGCGTCCGCCAGCAGAGCGCCGACCCCCCGGGTATTTGGGGGCCGCTCACTCAAACCCCATCCCCCCGGCCAtgt                                    >  2:442750/1‑135 (MQ=255)
                                                                                                     ccaccACTCAAAAATCCGCCCGCGGGGCGCTCGATTCACCCGCCACGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGACCACCCGGGTATTGGGGGTCAGCTCACTCATACCCCTGCACCCCGGCAATGTgccg                                <  8:49714/131‑1 (MQ=255)
                                                                                                      cAGCACTGAAAAATCAGACCGCGTGGCGCTCGATTCAAGCGCCCCGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGCCCCCCCGGGTATTGGGGGGCAGCGCACTCCTACCCCTGCCCCCCGGCAAAGTgccgc                               >  1:414401/1‑135 (MQ=255)
                                                                                                        gCACTGAAAAATCAGACCGCGTGGCGCTCGATTAAAGCGCCACGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCCACCACCCGGGTATTGGGGGTCCGCTCACACATACCCCTGCACCCAGGCAATGTGCCGccc                             >  8:320443/1‑135 (MQ=255)
                                                                                                         cACTGAAATATCAGACCGCGTGGCGCTCGATTCAAGCGCCACGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGACCACCCGGGTATTGGGGGTCAGCTCACTCATAc                                                       >  7:465394/1‑108 (MQ=255)
                                                                                                         cACTGAAATATCAGACCGCGTGGCGCTCGATTCAAGCGCCACGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGACCACCCGGGTATTGGGGGTCAGCTCACTCATAc                                                       <  8:465394/108‑1 (MQ=255)
                                                                                                           cttaaaaaTCCCAGCCCGTGCCGCTGGATGCAAGCGCCAGGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGACCACCCGGGTATTGGGGGTCAGCTCACTCATACCCCTGCACCCCGGCAATGTGCCGCCCGGc                          <  2:158467/132‑1 (MQ=255)
                                                                                                           cggAAAAATCCCCCGGCGGGGCGCGCTATGCACGCGCCACGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGACCACCCGGGTATTGGGGGTCAGCTCACTCATACCCCTGCACCCCGGCAATGTGCCGCCCGGc                          <  7:72234/133‑1 (MQ=255)
                                                                                                           cTGAAAATTCAGCCCGCGTGGGGCTCGATGCAAGCGCCACGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGACCAGCCGGGTATTGGTGGTCAGCTCACTCATACCCCTGCACCCCGGCAATGTGCCGCCCGGc                          <  3:349455/135‑1 (MQ=255)
                                                                                                           cTGAAAAATCAGACCGCGTGGCGCTCGATTCAAGCGCCACGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGACCACCCGGGTATTGGGGGTCAGCTCACTCATACCCCTGCACCCCGGCAATGTGCCGCCCGGc                          <  4:71462/135‑1 (MQ=255)
                                                                                                           cTGAAAAAACAGCCCGCGGGGCCCTCGATTCACGCGCCACGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGACCACCCGGGTATTGGGGGTCAGCTCACTCATACCCCTGCACCCCGGCAATGTGCCGCCCGGc                          <  2:257488/135‑1 (MQ=255)
                                                                                                                  atcaccccGCGGGGCGCTCGATTCACGCGCCACGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGACCACCCGGGTATTGGGGGTCAGCTCACTCATACCCCTGCACCCCGGCAATGTGCCGCCCGGCGACATAg                   <  1:243430/129‑1 (MQ=255)
                                                                                                                  agcccaCCGCGGGGCGCGCGATACACGCGCCACGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGACCACCCGGGTATTGGGGGTCAGCTCACTCATACCCCTGCACCCCGGCAATGTGCCGCCCGGCGACATAg                   <  3:462896/130‑1 (MQ=255)
                                                                                                                  aTCAGACCGCGTGGCGCTCGATTCAAGCGCCACGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGACCACCCGGGTATTGGGGGTCAGCTCACTCATACCCCTGCACCCCGGCAATGTGCCGCCCGGCGACATAg                   <  8:460505/135‑1 (MQ=255)
                                                                                                                  aTCACACCGCGTGGCGCTCGATACAAGCGCCACGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGACCACCCGGGTATTGGGGGTCAGCTCACTCATACCCCTGCACCCCGGCAATGTGCCGCCCGGCGACATAg                   <  4:36753/135‑1 (MQ=255)
                                                                                                                             tGGCGCTCGATTCACGCGCCACGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGACCACCCGGGTATTGGGGGTCAGCTCACTCATACCCCTGCACCCCGGCAATGTGCCGCCCGGCGACATAGCCAAAGGTCAg        <  8:374273/135‑1 (MQ=255)
                                                                                                                                   tCGATTCAAGGGCAACGGCCTTTCCCGGCGGCCTCCTGCAGAGCGCCGACCACCCGGGTATTGGGGGTCAGCTCACTCATACCCCTGCACCCCGGCAATGTGCCGCCCGGCGACATAGCCAAAGGTCAGCGCCgg  <  3:409913/135‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                      
AGCGCTTCCTGCGCACCGAGCAGATCGACGAGGTGGATGTGACCTACTGGCTGGCGCAAATTCAGGAGCGCAGCGCAGCGAGACCTGTGAACTGAGGGGTGACAGCACTGAAAAATCAGACCGCGTGGCGCTCGATTCAAGCGCCACGGCCTTTCCCGGCGTCCTCCTGCAGAGCGCCGACCACCCGGGTATTGGGGGTCAGCTCACTCATACCCCTGCACCCCGGCAATGTGCCGCCCGGCGACATAGCCAAAGGTCAGCGCCGG  >  ACB122/2965015‑2965280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: