Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | ACB122 | 690,144 | A→G | intergenic (+21/‑2438) | bauC → / → PP_RS08750 | Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | ACB122 | 690,144 | 0 | A | G | 92.9% | 13.3 / ‑5.8 | 15 | intergenic (+21/‑2438) | bauC/PP_RS08750 | Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val |
Reads supporting (aligned to +/- strand): ref base A (1/0); major base G (13/0); minor base T (1/0); total (15/0) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGAGGCCGTCAGGCC‑TCCCCGC‑TTTG‑TTTTTGGGGGTTGGTTGTTGCTGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCC > ACB122/690028‑690236 | ggTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGTGGCCGGCAGGCC‑TCCCCGcg > 8:95947/1‑136 (MQ=255) ggTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGAGGCCGTCCCTCC‑GCCCCGcc > 8:265723/1‑136 (MQ=255) taCCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCGGGCTGCC‑GCCCCGC‑GTTG‑TTTTTtggggg > 4:199450/1‑136 (MQ=255) aCCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGCCGGCAGGCCGCCCCCCC‑TTTG‑TTTTTTGGggt > 7:341682/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCGTCAGGCC‑GCCCCCC‑GTTG‑GTTTTGGGGGGTGggtgtt > 8:252427/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCGGGAGGGC‑GGCCCGC‑GGTG‑GTTTTTGGGGGTGggtgtt > 6:59790/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCGGGAGGGC‑GCCCCGCGGTGG‑TTTTTGGGggttggggtt > 2:229102/1‑133 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCGGGAGGGC‑ACCCCCC‑GTTG‑TTTTTTGGGGGTGgtggtt > 8:202475/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGCCGGGACGGC‑CCGCCCC‑GTTG‑TTTTTTTGGGGTGggtgtt > 7:331172/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGCCGGCACGCC‑ACCCCCC‑CTTG‑TTTTTTGGGGGTGgttgtt > 8:316700/1‑137 (MQ=255) aaGGCCATTTCCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGCCCGGGGGGC‑TCCCCCC‑GGTG‑TTTTTTGGGGGTGggtgtggg > 2:199646/1‑134 (MQ=255) aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGCGGGGCGGGC‑GGCCCCC‑GTTG‑TTTTTTGGGGGTGGttgttgg > 6:239627/1‑136 (MQ=255) aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGCCGGGAAGGC‑GCACCCC‑TGTGTTTTTTTGGGGTGGgttgtgg > 4:230906/1‑135 (MQ=255) gTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCGGCCCGCC‑GGCCCGC‑CGGG‑GTTTTTGGGGGTGGTTGGTG‑TGTTGgggggggggggg > 5:312652/1‑131 (MQ=255) cAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCGTGAGGGC‑GCCCCCCGGTGG‑TTTTTGGGGGTGGGTGTTTGTGGTTGGGGGGTAAAGGTTTTTGGGGGGGGTTGG‑TGTGTGAtttttga > 8:101703/1‑131 (MQ=255) | GGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGAGGCCGTCAGGCC‑TCCCCGC‑TTTG‑TTTTTGGGGGTTGGTTGTTGCTGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCC > ACB122/690028‑690236 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |