| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 570,451 | T→G | 37.4% | V600G (GTG→GGG) | fdoG_2 → | Formate dehydrogenase‑O major subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 570,451 | 0 | T | G | 37.4% | 18.2 / 3.5 | 35 | V600G (GTG→GGG) | fdoG_2 | Formate dehydrogenase‑O major subunit |
| Reads supporting (aligned to +/- strand): ref base T (15/6); new base G (7/7); total (22/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGGGCCTGGCCGGCCAACCGCCGCATCCTTTACAACCGTGCCTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCACCGACGTGCCGGACTTCAAGGTCGACTCGCCGCCCGAGGCGGGGATGAACCCTTTCATCATGAACCCCGAAGGCGTGGCGCGTTTCTTCGCCATCGACAAGATGGCCGAAGGCCCGTTCCCCGAGCACTACGAGCCCT > ACB122/570323‑570582 | ctgggcctggCCGGCCAACCGCCGCATCCTTTACAACCGTGCCTCCAGCGCCCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGGATGGTGGAACGGCAAGGCCTGGACCGGCACCGACGTGGCggcc > 4:292109/1‑134 (MQ=255)ctgggcctggCCGGCCAACCGCCGCATCCTTTACAACCGTGCCTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGAGCGGCACCGCCGGGCCGGa > 5:369477/1‑135 (MQ=255)ctgggcctggCCGGCCAACCGCCGCATCCTTTACAACCGTGCCTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCCCCgccgggccggcc > 7:392697/1‑134 (MQ=255)ctgggcctggCCGGCCAACCGCCGCATCCTTTACAACCGTGCCTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCCCCgccgggccggc > 1:381564/1‑134 (MQ=255)ctgggcctggCCGGCCAACCGCCGCATCCTTTACAACCGTGCCTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCACCGCCGTGGCggc > 1:162771/1‑134 (MQ=255)ctgggcctggCCGGCCAACCGCCGCATCCTTTACAACCGTGCCTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCACCGACGTGCCgggc > 3:125586/1‑134 (MQ=255) cctggCCGGCCAACCGCCGCATCCTTTACAACCGTGCCTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCCCCgccgggccgggcgt > 1:70498/1‑129 (MQ=255) cctggCCGGCCAACCGCCGCATCCTTTACAACCGTGCCTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCCCCGACGTGCCGGAcgt > 6:379891/1‑131 (MQ=255) ggccAACCGCCGCATCCTTTACAACCGTGCCTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCACCGCCGGGGCGGACGTCAAGGTCGAc > 4:219920/1‑136 (MQ=255) aaCCGCCGCATCCTTTACAACCGTGCCTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCCCCCCCGTGCCGGCCTGCAAGGTCGACTcgc > 8:247949/1‑136 (MQ=255) ccacccTTTACAACCGGGCCTCCTCCCACCCCCAAGGCACGCCGGGGGCCCCGCATAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCACCGACGTGCCGGACTTCAAGGTCGACTCGCCGCCCGa < 8:397060/132‑1 (MQ=255) aaCCGTGCCTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGAGCCGCCCCCCCGGGCCGGCCGTCAAGGTGGACTCCCCCCCCCCCGCGGGGGTg > 1:360251/1‑135 (MQ=255) aaCCGTGCCTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCCCCGCCGTGCCGGACTTCAAGGTCGACTCGCCGCCCGCGGCGGGGATg > 6:278401/1‑135 (MQ=255) aaCCGTGCCTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCCCCGCCGGGCCGGCCGTCAAGGTCGGCGCGCCGCCCGCGGCGGGGCTg > 5:129266/1‑135 (MQ=255) cGTGCCCCCCCCCACCCCAAACGCACGCGGGGGGACCCGGATAACAAGCGCCTGGTATGGTGGACGGGCAAGGCCGGGACCGGCACCGTCGTGGCGGACTTCAAGGTCGACTCGCCGCCCGAGGCGGGGATGAAcc < 4:355781/136‑1 (MQ=255) tGCCTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCCACGAACTGGCGGCCGTAAAGGTCGACTCGCCGCCCGACGCGGGGAAGAAccc > 6:349219/1‑135 (MQ=255) cTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCCCCGCCGGGCCGGGCGTCAAGGTCGCCTCGCCGCCCGAGGCGGGGATGAAcccga > 1:346093/1‑132 (MQ=255) cTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCACCGCCGTGCCGGACTTCAAGGTCGACACGCCGCCCGAGGCGGGGCAGAAcccagt > 4:56915/1‑132 (MQ=255) cTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCACCGCCGTGCCGCACTTCAAGGTCGACTCGCCGCCCGCGGCGGGGATGAACCCttt > 7:220172/1‑135 (MQ=255) cTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCACCGCCGGGCCGGACTTCAAGGTCGCCTCGCCGCCCGAGGCGGGGATGAACCCttt > 7:305221/1‑135 (MQ=255) cTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCACCGACGTGCCGGACTTCAAGGTCGACTCGCCGCCCGAGGCGGGGATGAACCCtt > 2:97843/1‑134 (MQ=255) cTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCACCCCCGGGCCGGACGTCAAGGTCGACTCGCCGCCCGCGGCGGGGATGAACCCttt > 3:184250/1‑135 (MQ=255) cTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCACCCACGTGCCGGGCCTAAAAGTCGACTCCCCCCCCCACGCGGGGGGGAACCCgtt > 7:357677/1‑135 (MQ=255) ccAGCGCCCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCACCGCCGTGCCGGCCTTCAAGGTCGACTCGCCGCCCGAGGCGGGGATGCACCCTTtca > 6:199354/1‑135 (MQ=255) cccgccccGCACGCCACGCGGGGGGACCCGTATAAACCGCGCCTGTTATGGTGGAACGGCAAGGCCGGGACCGGCACCGACGTGCCGGACTTCAAGGTCGACTCGCCGCCCGAGGCGGGGATGAACCCTTtcatc < 1:35705/130‑1 (MQ=255) gcaaggcaagCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCCCCGACGTGCCGGCCGTCAAGGTCGCCTCGCCCCCCGCGGCGGGGCTGGACCCGTTGATCATGAAcccc > 3:40415/1‑136 (MQ=255) acgCCGTGGGACCCGCACAAAACGCGCCTGGTATGGTGGAACGGCAAGGCCGGGCCCGGCACCGACGGGCCGGACTTCAAGGTCGACTCGCCGCCCGAGGCGGGGATGAACCCTTTCATCATGAACCCCGAAGGCg < 7:267328/134‑1 (MQ=255) gTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCACCCACGTGCCGGACTTCAACGTAGACTCGCCGCCCGAGGCGGGGATGAACCCGTTCATCATGAACCCCGAACGCGTGgcg > 4:253217/1‑136 (MQ=255) cgcCGGGTTTGGGGCAACGGCACGGCCTGGACCGGCACCGCCGGGCCGGACTTCAAGGTCGACTCGCCGCCCGAGGCGGGGATGAACCCTTTCATCATGAACCCCGAAGGCGTGGCGCGTTTCTTCGCCATCGACa < 3:79348/136‑1 (MQ=255) ggCCAGGCCGGGCCCGGCACCGACGGGCCGGACTTCAAGGTCGACTCGCCGCCCGAGGCGGGGATGAACCCTTTCATCATGAACCCCGAAGGCGTGGCGCGTTTCTTCGCCATCGACAAGATGGCCGAAGGCCCGt < 8:305221/136‑1 (MQ=255) ggCACGGCGTGCACCGGCACCGACGGCCGGGACTTCAAGGTCGACGCGCCGCCCGAGGCGGGGATGAACCCTTTCATCATGAACCCCGAAGGCGTGGCGCGTTTCTTCGCCATCGACAAGATGGCCGAAGGCCCGt < 3:369306/136‑1 (MQ=255) aGGCCTGGACCGGCACCGACGTGCCGGGCTTCAAGGTCGACTCGCCGCCCGCGGCGGGGATGAACCCTATAATCATGAACCCCGAAGGCGTGGGGCGTTTGTTAGCCATCCAAAACATGGCCCAACGCCCGGTccc > 8:35574/1‑136 (MQ=255) gggcTGGACCGGCACCGAGGGGCCGGACTTCAAGGTCGACTCGCCGCCCGAGGCGGGGATGAACCCTTTCATCATGAACCCCGAAGGCGTGGCGCGTTTCTTCGCCATCGACAAGATGGCCGAAGGCCCGTTcccc < 7:13204/133‑1 (MQ=255) aCCGGCACCGACGTGCCGGACTTCAAGGTCGACTCGCCGCCCGAGGCGGGGATGAACCCTTTCATCATGAACCCCGAAGGCGTGGCGCGTTTCTTCGCCATCGACAAGATGGCCGAAGGCCCGTTCCCCGAGCACt < 4:15801/136‑1 (MQ=255) ccGGCACCGCCGGGCCGGACTTCAAGGTCGACTCGCCGCCCGAGGCGGGGATGAACCCTTTCATCATGAACCCCGAAGGCGTGGCGCGTTTCTTCGCCATCGACAAGATGGCCGAAGGCCCGTTCCCCGAGCACt < 2:360251/135‑1 (MQ=255) ccGGCACCGACGGGCCGGACTGCAAGGTCGACTCGCCGCCCGAGGCGGGGATGAACCCTTTCATCATGAACCCCGAAGGCGTGGCGCGTTTCTTCGCCATCGACAAGATGGCCGAAGGCCCGTTCCCCGAGCACt < 2:100341/135‑1 (MQ=255) gCACCGACGTGCCGGACTTCAAGGTCGACTCGCCGCCCGAGGCGGGGATGAACCCTTTCATCATGAACCCCGAAGGCGTGGCGCGTTTCTTCGCCATCGACAAGATGGCCGAAGGCCCGTTCCCCGAGCACTACGa < 4:185719/136‑1 (MQ=255) aCGTGCCGGACTTCAAGGTCGACTCGCCGCCCGAGGCGGGGATGAACCCTTTCATCATGAACCCCGAAGGCGTGGCGCGTTTCTTCGCCATCGACAAGATGGCCGAAGGCCCGTTCCCCGAGCACTACGAGCCCt < 1:97843/135‑1 (MQ=255) | CTGGGCCTGGCCGGCCAACCGCCGCATCCTTTACAACCGTGCCTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCACCGACGTGCCGGACTTCAAGGTCGACTCGCCGCCCGAGGCGGGGATGAACCCTTTCATCATGAACCCCGAAGGCGTGGCGCGTTTCTTCGCCATCGACAAGATGGCCGAAGGCCCGTTCCCCGAGCACTACGAGCCCT > ACB122/570323‑570582 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |