Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	1,178,758	T→C	6.7%	S304P (TCC→CCC)	purL →	Phosphoribosylformylglycinamidine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	1,178,758	0	T	C	6.7%	218.5 / 4.9	75	S304P (TCC→CCC)	purL	Phosphoribosylformylglycinamidine synthase
Reads supporting (aligned to +/- strand):  ref base T (35/33);  new base C (2/3);  total (37/38)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.36e-01

GGTGATCGTCGGCAACGTTGCCGGCCGTTTCTTCCCGAACCCTGAAACCCGCCAGTACGGCGCGGTGCAGGAGCCGGTGCACATCCTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTACCGGCCGGGGCGCCAAGCCCAAGGCCGGCCTGACCGGCTTCACCGTGTCCAACCTGCGCATTCCGGGCTTCGAACAGCCTTGGG  >  ACB122/1178625‑1178890                                                                                                                                       |                                                                                                                                     ggTGATCGTCGGCAACGTTGCCGGCCGTTTCTTCCCGAACCCTGAAACCCGCCAGTACGGCGCGGTGCAGGAGCCGGTGCACATCCTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTtctc                                                                                                                                     <  8:295141/135‑1 (MQ=255) ggTGATCGTCGGCAACGTTGCCGGCCGGTTCTTCCCGAACCCTGAAACCCGCCAGTACGGCGCGGTGCAGGAGCCGGTGCACATCCTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTtctc                                                                                                                                     <  8:289537/135‑1 (MQ=255)   tGAGCGCCGGCAACGGTGCCGGCCGTTTCTTCCCGAACCCTGAAACCCGCCAGTACGGCGCGGTGCAGGAGCCGGTGCACATCCTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCgg                                                                                                                                  <  7:163067/136‑1 (MQ=255)             cAACGTTGCCGGCCGTTTCTTCCCGCACCCTGAAACCCGCCAGTACGGCGCGGTGCAGGAGCCGGGGCACATCCTGCTGAAGGTGGAAACGCACAA‑CCCCCCGCCCGCCATCGCCCCGTTCTCCGGCGCCTCcccc                                                                                                                        >  4:73309/1‑136 (MQ=255)                               cttcCCGAACCCTGAAACCCGCCAGTACGGCGCGGTGCAGGAGCCGGTGCACATCCTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCCCCGGCGCCTCCACCGGCTCCGGTGGCGAAATc                                                                                                      >  3:285274/1‑136 (MQ=255)                               cttcCCGAACCCTGAAACCCGCCAGTACGGCGCGGTGCAGGAGCCGGTGCACATCCTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCCCCGGCGCCTCCACCGGCTCCGGAGGCGCAATc                                                                                                      >  3:280204/1‑136 (MQ=255)                               ctccccGAACCCTGACACCCGCCCGTACGGCGCGGTGCAGGAGCCGGTGCACATCCTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAAt                                                                                                       <  8:146433/132‑1 (MQ=255)                                    cGAACCCTGAAACCCGCCAGTACGGCGCGGTGCAGGAGCCGGTGCACATCCTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGcga                                                                                                 <  7:68522/136‑1 (MQ=255)                                     gAACCCTGAAACCCGCCAGTACGGCGCGGTGCAGGAGCCGGTGCACATCCTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCcgcgc                                                                                                 >  6:181151/1‑134 (MQ=255)                                      aaCCCTGAAACCCGCCAGTACGGCGCGGTGCAGGAGCCGGTGCACATCCTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGcgacg                                                                                               <  8:199834/136‑1 (MQ=255)                                             aaaCCCGCCAGTACGGCGCGGTGCAGGAGCCGGTGCACATCCTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGc                                                                                        <  4:280223/136‑1 (MQ=255)                                                cccGCCAGTACGGCGCGGTGCAGGAGCCGGTGCACATCCTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTa                                                                                      >  5:258122/1‑135 (MQ=255)                                                    ccAGTACGGCGCGGTGCAGGAGCCGGTGCACATCCTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTAccgg                                                                                  <  2:49389/135‑1 (MQ=255)                                                     cAGTACGGCGCGGTGCAGGAGCCGGTGCACATCCTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTAccggcc                                                                                >  4:389341/1‑136 (MQ=255)                                                          cGGCGCGGTGCAGGAGCCGGTGCACATCCTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCCCCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTACCGGCCGGGgc                                                                           <  4:71687/136‑1 (MQ=255)                                                          cGGCGCGGTGCAGGAGCCGGTGCACATCCTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTGCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTACCGGCCGGGgc                                                                           <  4:166880/136‑1 (MQ=255)                                                           ggCGCGGTGCCGGCCCCGCTCCCCACCCTGATGACGGGGGAACCCCCCAA‑CCCCCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTACCGGCCGGGgc                                                                           <  5:383263/135‑1 (MQ=255)                                                           ggCGCGGTGCAGGAGCCGGTGCACATCCTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTACCGGCCGGGgc                                                                           <  6:271151/135‑1 (MQ=255)                                                                ggTGCAGGAGCCGGTGCACATCCTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAACTCCGCGACGAAGGTGCTACCGGCCGGGGCGCCAAg                                                                     >  4:62822/1‑136 (MQ=255)                                                                ggTGCAGGAGCCGGTGCACATCCTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTACCGGCCGGGGCGCCaa                                                                      >  1:2772/1‑135 (MQ=255)                                                                ggTGCAGGAGCCGGTGCACATCCTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTACCGGCCGGGGCGCCAAg                                                                     >  3:346605/1‑136 (MQ=255)                                                                    cAGGAGCCGGTGCACATCCTCATGACGGTGGAACCGCCCAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCGCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTACCGGCCGGGGCGCCAAGCCCa                                                                 <  3:122341/136‑1 (MQ=255)                                                                     aGGAGCCGGTGCACATCCTGATGAAGGTGGCAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGCAATCCGCGCCGAAGGTGCCACCGGCCGGGGCGCCACGccc                                                                  >  8:225146/1‑134 (MQ=255)                                                                              tGCACATCCTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTACCGGCCGGGGCGCCAAGCCCAAGGCCGGCCt                                                       <  8:332813/136‑1 (MQ=255)                                                                              tGCACATCCTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCCCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTACCGGCCGGGGCGCCAAGCCCAAGGCCGGCCt                                                       <  4:285274/136‑1 (MQ=255)                                                                              tGCACATCCTGATGAAGGCGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCCACCGGCCGGGGCGCCAAGCCCAAGGCCGGCCt                                                       <  7:225146/136‑1 (MQ=255)                                                                              ggcccaTCCTGATGAAGTGGCAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTACCGGCCGGGGCGCCAAGCCCAAGGCCGGCCt                                                       <  3:193048/132‑1 (MQ=255)                                                                              ggCACATCCTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCCCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGATACCGGCCGGGGCGCCAAGCCCAAGGCCGGCCt                                                       <  4:280204/135‑1 (MQ=255)                                                                               gCACATCCTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCTTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCTCGACGAAGGTGCTACCGGCCGGGGCGCCAAGCCCAAGGCCGGCCt                                                       >  8:244391/1‑135 (MQ=255)                                                                               gCACATCCTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGCAATCCGCGACGAAGGTGCTCCCGGCCGGGGCGCCAAGCCCAAGGCCGGCCTg                                                      >  3:23560/1‑136 (MQ=255)                                                                               gCACATCCTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAAt                                                                                                       <  2:76772/87‑1 (MQ=255)                                                                               gCACATCCTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAAt                                                                                                       >  1:76772/1‑87 (MQ=255)                                                                               gCACATCCTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTACCGGCCGGGgc                                                                           >  7:397282/1‑115 (MQ=255)                                                                               gCACATCCTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTACCGGCCGGGgc                                                                           <  8:397282/115‑1 (MQ=255)                                                                               gCACATCCTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTACCGGCCGGGGCGCCAAGCCCAAggccggcc                                                        >  1:28885/1‑134 (MQ=255)                                                                               gCACATCCTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTACCGGCCGGGGCGCCAAGCCCAAGGCCGGCCt                                                       >  8:233957/1‑135 (MQ=255)                                                                               gCACATCCTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTACCGGCCGGGGCGCCAAGCCCAAGGCCGGCCt                                                       >  7:71483/1‑135 (MQ=255)                                                                               gCACATCCTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTACCGGCCGGGGCGCCAAGCCCAAGGCCGGCCt                                                       >  8:123067/1‑135 (MQ=255)                                                                               gCACATCCTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTACCGGCCGGGGCGCCAAGCCCAAGGCCGGCCt                                                       >  1:374363/1‑135 (MQ=255)                                                                               gCACATCCTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTACCGGCCGGGGCGCCAAGCCCAAGGCCGGCCTg                                                      >  8:89173/1‑136 (MQ=255)                                                                               gCACATCCTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTACCGGCCGGGGCGCCAAGCCCAAGGCCGGCCTg                                                      >  4:38455/1‑136 (MQ=255)                                                                               gCACATCCTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTACCGGCCGGGGCGCCAAGCCCAAGGCCGGCCTg                                                      >  3:136277/1‑136 (MQ=255)                                                                               gCACATCCTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAACGTGCTACCGGCCGGGGCGCCAAGCCCAAGGCCGGCCTg                                                      >  3:24620/1‑136 (MQ=255)                                                                               gCACATCCTGAAGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCACCCCCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTACCGTCCGGGGCGCCAAGCCCAAcgccggcc                                                        >  2:214201/1‑134 (MQ=255)                                                                                actcaccTGTTGAAGGGGGAAACCCCCAACCCCCCCGACCCCCACCCCCCCGTTCTCCGGCGCCCCCCCGGGCGCGGGGGGCTAAACCCGCGCCGAAGGTGCTACCGGCCGGGGCGCCAAGCCCAAGGCCGGCCt                                                       <  6:307000/130‑1 (MQ=255)                                                                                      cTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTACCGGCCGGGGCGCCAAGCCCAAGGCCGGCCTGACCGGCt                                               >  3:320314/1‑136 (MQ=255)                                                                                      cTGATGAAGG‑GGGAACGCACAA‑CCACCCGACCGCCATCGCCCCGTGCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTACCGGCCGGGGCGCCAAGCCCAAGGCCGGCCTGACCGGCtt                                              <  4:57550/136‑1 (MQ=255)                                                                                       tgatgaCGGTGGACCCGCACAC‑CCACCCGACCGCCATCGCCCGGTCCGCCGGCGCCTCCACCGGCTCCGGGGGCGAAAGCGGCGACGAAGGTGCTACCGGGCGGGGCGCCAAGCCCAAGGCCGGCCTGACCGGCtt                                              <  8:388288/136‑1 (MQ=255)                                                                                        gtttacggTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGATGGTGCTACCGGCCGGGGCGCCAAGCCAAAGGCCGGCCTGACCGGCtt                                              <  6:1061/129‑1 (MQ=255)                                                                                        gatgaAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGCAATCCGCGACGAAGGTGCTACCGGCCGGGGCGCCACGCCCCAGGCCGGCCTGACCGGCtt                                              >  2:318152/1‑135 (MQ=255)                                                                                         atgaAGGTGGAAACCCACAA‑CCCCCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCCCCGGCTCCGGGGGCGAAAGCCGCGACGAAGGGGCTACCGGCCGGGGCGCCAAGCCCAAGGCCGGCCTGACCGGCtt                                              <  3:389341/134‑1 (MQ=255)                                                                                          tgaCGGTGCAACCGCACAC‑CCACCCGACCGCCAGCGCCCCGTTCGCCGGCGCCGCCCCCGGCTCCGGTGGCGAAATCCGCGACGAAGGGGCTACCGGCCGGGGCGCCAAGCCCAAGGCCGGCCTGACCGGCtt                                              <  5:181151/133‑1 (MQ=255)                                                                                           taaGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTATCCGGAGCCTCCACAGTCTCAGGTGGCGAAATCCCCGCCGAAGGTGCTACCGGTCGGGGCGCCAATCCCCAGGCAGTCCTGCCCGGCTTCAcc                                          >  8:83972/2‑136 (MQ=255)                                                                                           gaAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTACCGGCCGGGGCGCCAAGCCCAAGGCCGGCCTGACCGGCTTcccc                                          >  4:32494/1‑136 (MQ=255)                                                                                           gaAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTACCGGCCGGGGCGCCAAGCCCAAGGCCGGCCTGACCGGCTTCAcc                                          >  3:136330/1‑136 (MQ=255)                                                                                           gaAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTACCGGCCGGGGCGCCAAGCCCAAGGCCGGCCTGACCGGCTTCAcc                                          >  4:259189/1‑136 (MQ=255)                                                                                            aaGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTCCCGGCCGGGGCGCCAAGCCCAAGGCCGGCCTGACCGGCTTCACCg                                         >  3:358144/1‑136 (MQ=255)                                                                                              gggggAAACGCACAA‑CCCCCCGCCCGCCATCGCCCCGTGCTCCGGCGCCTCCCCCGGCGCCGGTGGCGAAATCCGCGACGAAGGGGCTACCGGCCGGGGCGCCAAGCCCAAGGCCGGCCTGACCGGCTTCACCgt                                        <  6:82036/132‑1 (MQ=255)                                                                                                tGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTACCGGCCGGGGCGCCAAGCCCAAGGCCGGCCTGACCGGCTTCACCgtgt                                      >  6:38625/1‑135 (MQ=255)                                                                                                 ggAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTACCGGCCGGGGCGCCAAGCCCAAGGCCGGCCTGACCGGCTTCACCgctgt                                     >  5:307000/1‑131 (MQ=255)                                                                                                      cGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTACCGGCCGGGGCGCCAAGCCCAAGGCCGGCCTGACCGGCTTCACCGTGTCCAACCt                               >  7:134739/1‑136 (MQ=255)                                                                                                      cGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCCGTCGCGAAATCCGCCACGAAGGTGCTACCGGCCGGGGCGCCACGCCCAACGCCGGCCTCACCGGCTTCACCGTGTCCAAcc                                >  5:189515/1‑135 (MQ=255)                                                                                                                     aCCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTACCGGCCGGGGCGCCAAGCCCAAGGCCGGCCTGACCGGCTTCCCCGTGTCCAACCTGCGCATTCCGGGc                  >  1:7244/1‑135 (MQ=255)                                                                                                                       cgcccgcGCCCCGTGCTCGGGCGCCCCCACCGGAGCGGGGGGCGAAATCCGCGCCGACGGGGCACCGGGCCGGGGCGCCATGCCCAAGGCCGGCCTGACCGGCTTCACCGTGTCCAACCTGCGCATTCCGGGCTTc               <  3:377537/130‑1 (MQ=255)                                                                                                                       cGCCCTCGCCCCGTGCTCCGGCGCCTCCCCCGGCGCCGGTGGCGAAATCCGCGACGAAGGGGCTCCCGGCCGGGGCGCCAAGCCCAAGGCCGGCCTGACCGGCTTCACCGTGTCCAACCTGCGCATTCCGGGCtt                <  6:87018/135‑1 (MQ=255)                                                                                                                       cGCCATCGCCCGGTTCTCCGGCGCCTCCACCGGCTCGGGTGTCTAAATCCGCGACGAAGGTGCTACCGGCCGGGGCGCCAAGCCCAAGGCCGGCCTGACCGGCTTCACCGTGTCCAACCTGCGCATTCCGGGCTTc               <  3:152915/136‑1 (MQ=255)                                                                                                                       cGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTACCGGCCGGGGCGCCAAGCCCAAGGCCGGCCTGACCGGCTTCACCGTGTCCAACCTGCGCATTCCGGGCTTc               <  8:38674/136‑1 (MQ=255)                                                                                                                       cGCCAGCGCCCGGTGCTCCGGCGCCTCCCCCGGCTCGGGTGGCTAATCCCGCGACCAAGGGGCTACCGGCCGGGGCGCCAAGCCCAAGGCCGGCCTGACCGGCTTCACCGTGTCCAACCTGCGCATTCCGGGCTTc               <  3:377535/136‑1 (MQ=255)                                                                                                                        gCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTACCGGCCGGGGCGCCAAGCCCAAGGCCGGCCTGACCGGCTTCACCGTGTCCAACCTGCGCATTCCGGGCTTc               <  6:138325/135‑1 (MQ=255)                                                                                                                         ccATCGCCCCGTGCTCCGGCGCCTCCCCCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTACCGGCCGGGGCGCCAAGCCCAAGGCCGGCCTGACCGGCTTCACCGTGTCCAACCTGCGCATTCCGGGCTTCg              <  6:215035/135‑1 (MQ=255)                                                                                                                            tCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCAGCGACGAAGGTGCTACCGCCCGGGCCGCCCCGCCCAACGCCGGCCTGACCGGCTTCACCGTGTCCAACCACCGCATTCCCGGCTTCgc             >  5:202686/1‑132 (MQ=255)                                                                                                                               ccccGTGCCCGGCCGCCCCCCCGGGCGCGGGTGGCAAAAGCCGCCACGACGGGGCACCCGGCGGGGGCGCCACCCCCAAGGCCGGCCTGACCGGCTTCACCGTGTCCAACCTGCGCATTCCGGGCTTCGAACAGc        <  5:345521/135‑1 (MQ=255)                                                                                                                                   gTTCTCCGGCGCCTCCACGGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTACCGGCCGGGGCGCCAAGCCCAAGGCGGGCCTGACCGGCTTCACCGTGTCCAACCTGCGCATTCCGGGCTTCGAACAGCCTTgg   <  7:233957/136‑1 (MQ=255)                                                                                                                                   gTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTACCGGCCGGGGCGCCAAGCCCAAGGCCGGCCTGACCGGCTTCACCGTGTCCAACCTGCGCATTCCGGGCTTCGAACAGCCTTgg   <  4:320314/136‑1 (MQ=255)                                                                                                                                   gTTCTCCGGCGCCTCCACCGGCGCCGGTGGCGAAATCCGCGACGAAGGTGCTACCGGCCGGGGCGCCAAGCCCAAGGCCGGCCTGACCGGCTTCACCGTGTCCAACCTGCGCATTCCGGGCTTCGAACAGCCTTgg   <  8:3733/136‑1 (MQ=255)                                                                                                                                    ttCTCCGGCGCCTCCACCGGCTCCGGTGGCGAATTCCGCTACTAAGGTGCTACCGGCCGGGGCGCCAAGCCCAAGGCGGGCCTGACCGGCTTCACCGTGTCCAACCTGCGCATTCCGGGCTTCGAACAGCCTTggg  <  4:24620/136‑1 (MQ=255)                                                                                                                                    ttCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTACCGGCCGGGGCGCCAAGCCCAAGGCCGGCCTGACCGGCTTCACCGTGTCCAACCTGCGCATTCCGGGCTTCGAACAGCCTTgg   <  1:13577/135‑1 (MQ=255)                                                                                                                                    tgctcCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGATGAAGGTGCTCCCGGCCGGGGCGCCAAGCCCAAGGCCGGCCTGACCGGCTTCACCGTGTCCAACCTGCGCATTCCGGGCTTCGAACAGCCTTgg   <  6:187769/133‑1 (MQ=255)                                                                                                                                       |                                                                                                                                     GGTGATCGTCGGCAACGTTGCCGGCCGTTTCTTCCCGAACCCTGAAACCCGCCAGTACGGCGCGGTGCAGGAGCCGGTGCACATCCTGATGAAGGTGGAAACGCACAA‑CCACCCGACCGCCATCGCCCCGTTCTCCGGCGCCTCCACCGGCTCCGGTGGCGAAATCCGCGACGAAGGTGCTACCGGCCGGGGCGCCAAGCCCAAGGCCGGCCTGACCGGCTTCACCGTGTCCAACCTGCGCATTCCGGGCTTCGAACAGCCTTGGG  >  ACB122/1178625‑1178890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑