Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 4,134,647 T→G 17.0% intergenic (+33/‑36) CJ019_03892 → / → tgnD hypothetical protein/(E)‑2‑((N‑methylformamido)methylene)succinatehydr olase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1224,134,6470TG17.0% 118.3 / 2.3 64intergenic (+33/‑36)CJ019_03892/tgnDhypothetical protein/(E)‑2‑((N‑methylformamido)methylene)succinatehydr olase
Reads supporting (aligned to +/- strand):  ref base T (16/34);  new base G (11/2);  total (28/36)
Fisher's exact test for biased strand distribution p-value = 1.07e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTCCTGGGCGCAGCCGATGGCGGCCGCCTGCACCCGCGCATCGGCAACCGTTACATCGACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACGGCGTG  >  ACB122/4134515‑4134772
                                                                                                                                    |                                                                                                                             
gggccTGGGCGCAGCCGATGGCGGCCGCCGGCACCCGCGCATCGGCAACCGTTACATCGACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCGCAATAACAACACCCAAGACGGGCCGGGGCGTGGCGTGCTTAc                                                                                                                            <  8:273828/133‑1 (MQ=255)
          gcAGCCGCTGGCGGCCGCCTGCACCCGCGCATCGGCCACCGTTACATCGACCGGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAAGAACACCAACCCAGACGGGCCGGGGCGTGGCGGGCTTGCCCGCCGCGcc                                                                                                                  >  8:313538/1‑136 (MQ=255)
          gcAGCCGATGGCGGCCGCCTGCACCCGAGCATCGGCAACCGTTAAATCGACCTGGAAGAACATGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCCGGGCGTGGCGTTCTTACCCGCCgcgc                                                                                                                   >  6:108832/1‑135 (MQ=255)
            aGCCGATGGCGGCCGCCTGCACCCGCGCATCGGCAACCGTTACATCGACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACCACCAAGACGGGCCGGGGCGTGGCGTGGTTTCCCGCCGCGgcc                                                                                                                 >  1:216062/1‑135 (MQ=255)
               cGATGGCGGCCGCCTGCACCCGCGCATCGGCAACCGTTACATCGCCCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCCGGGCCGGCGTTCCCCCCCCGccccccc                                                                                                             >  7:59958/1‑132 (MQ=255)
               cGATGGCGGCCGCCTGCACCCGCGCATCGGCAACCGTTACATCGACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACCACAACCAAGACGGGCCGGGGCGTGGCGGGCGTACCCGCCGCgcccgccc                                                                                                             >  4:74143/1‑136 (MQ=255)
               cGATGGCGGCCGCCTGCACCCGCGCATCGGCAACCGTTACATCGACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGGGGGGGTTCCCCCCGCGCCCgcc                                                                                                              >  5:391399/1‑133 (MQ=255)
               cGATGGCGGCCGCCTGCACCCGCGCATCGGCAACCGTTACATCGACCTGGAAGAACTGGCTGCCGAAAAACCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGGGCGTACCcgccccgccccccc                                                                                                             >  8:12472/1‑132 (MQ=255)
               cGATGGCGGCCGCCTGCACCCGCGCATCGGCAACCGTTACATCGACCGGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCgcccgccc                                                                                                             >  4:173732/1‑136 (MQ=255)
               cGATGGCGGCCGCCTGCACCCGCGCATCGGCAACCGTTACATAGACCTGGCAGAACTGGCTGGCGAAAAAGCCAACGGTCCATAACAACAACCAAGACGGGCCGGGGCGTGGGGTTGGTTCCCCCCGCGccccgcc                                                                                                             >  8:72770/1‑132 (MQ=255)
                 aTGGCGGCCGCCTGCACCCGCGCATCGGCAACCGTTACATCGACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCGTACCCGCCGCgcccgccc                                                                                                             >  2:260998/1‑134 (MQ=255)
                    gcggcCGCCTGCACCCGCGCATCGGCAACCGTTACATCGACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCaa                                                                                                        <  7:329476/136‑1 (MQ=255)
                     cggcCGCCTGCACCCGCGCATCGGCAACCGTTACATCGACCTGGAAGATCTGGCTGCGGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAg                                                                                                       <  7:67505/136‑1 (MQ=255)
                      ggcCGCCTGCACCCGCGCATCGGCAACCGTTACATCGACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGGGTGCGTACCCGCCCCGCCCCGCCGTCaaag                                                                                                      >  3:68962/1‑134 (MQ=255)
                       gccgccTGCACCCGCGCATCGGCAACCGTTACTTCGACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGa                                                                                                     <  8:373957/136‑1 (MQ=255)
                         cgccTGCACCCGCGCATCGGCAACCGTTACATCGACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGGGTGCTTACCCGCCGCGGCCGCCCGTCAAGgcgc                                                                                                   >  3:260462/1‑136 (MQ=255)
                         cgccTGCACCCGCGCATCGGCAACCGTTACATCGACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAgc                                                                                                   <  8:298048/136‑1 (MQ=255)
                           ccTGCACCCGCGCATCGGCAACCGTTACATCGACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGCCCTTCAAGGAgcgc                                                                                                 >  4:291037/1‑136 (MQ=255)
                               cACCCGCGCATCGGAAACCGTTACATCGACCTGGAAGAACTGGCTGCCGAAAAACCCAACGCTCTATAACAACAACCTCGACGGTCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGTAGCGccc                                                                                               <  4:110763/134‑1 (MQ=255)
                                       cATCGGCAACCGTTACATCGACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGAtt                                                                                      >  8:135139/1‑135 (MQ=255)
                                        aTCGGCAACCGTTACATCGCCCTGGACGCCCGGGCGGCCGAAAAAGCCAACGCTCAATAACACCAACCACGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTc                                                                                     <  1:41479/135‑1 (MQ=255)
                                          cGGCAACCGTTACATCGCCCTGGAAGAACTGGCGGCCGAAAACGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGc                                                                                  <  8:314403/136‑1 (MQ=255)
                                            gCAACCGTTACATCGACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGCCCGTCAAGGCGCGCCCGCCCTGCTTCCGCCt                                                                                >  3:100143/1‑136 (MQ=255)
                                             cAACCGTTACATCGACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGGGCTTACCCCCCGCGCCCCCCCGTCAAGGACCGGCCACCATGGTTCAGccag                                                                               >  4:257455/1‑134 (MQ=255)
                                                ccGTTACATCGACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTc                                                                             <  2:274251/135‑1 (MQ=255)
                                                ccGTTACATCGACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTc                                                                             <  2:274249/135‑1 (MQ=255)
                                                        tCGACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGc                                                                    <  4:245657/136‑1 (MQ=255)
                                                          gcccTGCAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCCACACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGca                                                                   <  6:338743/133‑1 (MQ=255)
                                                          gACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGcac                                                                  >  4:140796/1‑136 (MQ=255)
                                                            ccTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCacac                                                                <  3:140796/136‑1 (MQ=255)
                                                              tcgaagaaCTGGCTGCCCAAAAAGCCAACGCTCAATAACAACAACCACGACGGGCCGCGGCCTGCCGTTGATACCCCCCGCGCCCGACCATACATGAACGCACACCATGATTAAGACTATCCCAGAACCCACAcca                                                              >  4:155717/3‑135 (MQ=255)
                                                              tGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTTGCGAGCTTACCCGCCCCGCCCGACCTTAAAGCAGCCCCCCACAAGAATCAACCAGTCACAGAACGccca                                                                 >  2:204934/1‑133 (MQ=255)
                                                                      ctgcctgcCCAAAACCCCAACGCTCAAAAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCAc                                                      <  3:234543/136‑1 (MQ=255)
                                                                       tGGCTCCCTAAAAAGCCAACGCTCAAACACAACAACCACGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCAc                                                      <  1:147918/135‑1 (MQ=255)
                                                                         gCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGCCCTTCAAGGCGCGCCCTCCCTGATTAAGCCTGTCGCTGGACGCCCCCCGGCCGGCACCac                                                   >  7:385332/1‑135 (MQ=255)
                                                                            gCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGGGTTCCCCCCCCGCCCCCCCGTCAACGCGCGGCCGCCCAGATTAAGCCTGTCGCGGCAAGCCCCCCAGCCGGCACCCGcga                                                >  3:348211/1‑134 (MQ=255)
                                                                             ccGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGGGCGTAACCCCCCCCCCCCCCCGTCAACGACCCCCCACCAAGATAAAGCCGGTCGCAGAACCCACACCAGCCCGCACCAGcca                                                >  4:114674/1‑133 (MQ=255)
                                                                                 aaaaGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTgg                                           <  8:260329/136‑1 (MQ=255)
                                                                                  aaaGCCAACGCTCAATAACAACAACCACGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTgg                                           <  2:38930/135‑1 (MQ=255)
                                                                                      ccAACGCTCAATAACAACAACCAAGACTGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTc                                      <  8:118342/136‑1 (MQ=255)
                                                                                       cAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTc                                      <  6:311016/135‑1 (MQ=255)
                                                                                              cAATACCAACACCCACGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAggg                              <  3:226031/136‑1 (MQ=255)
                                                                                                   ccaacaccCAAGACGGGCCGGGGCGTGGCGTGCTGACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAAc                         <  8:74516/135‑1 (MQ=255)
                                                                                                   ccaacaacCCCGCCGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCGGGATGTCGGCCAGGGCCAAc                         <  7:12472/135‑1 (MQ=255)
                                                                                                    caacaacCACGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAAc                         <  2:159137/135‑1 (MQ=255)
                                                                                                    aaccacccACGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCCTGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAAc                         <  6:24414/129‑1 (MQ=255)
                                                                                                        aacCACGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCgtg                    <  4:348237/136‑1 (MQ=255)
                                                                                                          ccAAGACGGGCCGGGGCGTGGCGTGCGTACCCGCCCCGCCCGACCTTCAAGGAGCGCCCTACAAGATTCATCCAGTCGCTGTACCCCCCCCACCCCGCCCCCGCTATCTGGATTTCGGCCAGGGCCAACCCctgg                   >  2:332387/1‑135 (MQ=255)
                                                                                                            aaGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCGATATGGATGTCGGCCAGGGCCAACCCCTGGTGc                >  7:17956/1‑136 (MQ=255)
                                                                                                                cGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTGCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGAt            <  3:257455/136‑1 (MQ=255)
                                                                                                                cGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGAt            <  7:186612/136‑1 (MQ=255)
                                                                                                                cGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGAt            <  7:87817/136‑1 (MQ=255)
                                                                                                                 gggCCGGGGCGTGGCGGGAGCACCCCCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGAt            <  5:232113/135‑1 (MQ=255)
                                                                                                                  ggCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCCCCCCTGCCGGCACCAGCGATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATc           >  3:94609/1‑135 (MQ=255)
                                                                                                                  ggCCGGGGCGTGGCGTGCTTACCCGCCCCGCCCGCCCATCAACGAGCCCCCTCCCTGATTCAGCCAGTCGCTGCACGCCCACCAGCCGGCCCCCGCGATCTGGATGTCGGCCAGGGCCCACCCGTGGTGCTGCTcc          >  8:52212/1‑136 (MQ=255)
                                                                                                                     cGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACg       <  7:46189/136‑1 (MQ=255)
                                                                                                                     cGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACg       <  7:135139/136‑1 (MQ=255)
                                                                                                                     cGGGGCGTGGCGTGCGTACCCGCCGCGCCCGCCCTTCAAGGCGCGCCCGCCAAGATTAAGCCGGTAGCGGGACGCACACCAGCCGGCACCAGCTATCTGGTTGTCGGCCACGGCCCACCCGTGGGGCGGGTCCAc        >  2:318986/1‑135 (MQ=255)
                                                                                                                      ggggCGTGGCGTGCTTCCCCGCCGCGCCCGACCTTCCAGGAGCGCCCTCCATGAATCAGCCTCGCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACg       <  5:252394/135‑1 (MQ=255)
                                                                                                                      ggggCGTGGCGTGCGTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCAGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACg       <  1:396861/135‑1 (MQ=255)
                                                                                                                        ggcgtggcgtGCTTACCCGCCGCGCCCGGCCGTCAAGGCGCGCCCGCCCTGATTCAGCCTGTCGCGGAACGCCCCCCTGCCGGCACCCGCGATCTGGGTGTCGGCCAGGGCCAACCCCTGGTGCTGCTCCCCGGCg    >  8:186612/1‑136 (MQ=255)
                                                                                                                          cgtggcgtgCTTACCCGCCGCGCCCGACCTGCACGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACGGCGTg  <  3:56307/136‑1 (MQ=255)
                                                                                                                           gtggcgtgCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACGGCGTg  <  1:214117/135‑1 (MQ=255)
                                                                                                                           gtggcgggCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACGAGCTATCTGGATGTCGGCCAGGGCCACCCCGTGGTGCTGATCCACGGCGTg  <  6:264623/135‑1 (MQ=255)
                                                                                                                                    |                                                                                                                             
GGTCCTGGGCGCAGCCGATGGCGGCCGCCTGCACCCGCGCATCGGCAACCGTTACATCGACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACGGCGTG  >  ACB122/4134515‑4134772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑