Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | ACB122 | 5,226,991 | T→G | 9.8% | intergenic (‑26/‑132) | CJ019_04921 ← / → fecI_10 | hypothetical protein/putative RNA polymerase sigma factor FecI |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | ACB122 | 5,226,991 | 0 | T | G | 9.8% | 149.7 / 2.7 | 60 | intergenic (‑26/‑132) | CJ019_04921/fecI_10 | hypothetical protein/putative RNA polymerase sigma factor FecI |
Reads supporting (aligned to +/- strand): ref base T (20/33); new base G (5/2); total (25/35) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.17e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GACACTGGCCATCGACGCCAGCAAGTAGCCGCCCAGCAGCGCGGCCAGGCTGCGTGAGGCAACGCCCAGCCGGTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTTTTCGGGGTTGCAATACTAATGATTTCAATTCTCATAAGCAAAGCCTGCTGACAGGTGACGATTGCCGAAACAGACGGCCACCCATACAATGCGAACAATTCTTACTACCAGTTGCGCTGTCATGCGCCGGAGTATCA > ACB122/5226858‑5227120 | gACACTGGCCATCGACGCCAGCAAGTAGCCGCCCAGCAGCGCGGCCAGGCTGCGTGAGGCAACGCCCAGCCGGTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTTTTCGGGGTTg < 7:306568/136‑1 (MQ=255) gACACTGGCCATCGACGCCAGCAAGTAGCCGCCCAGCAGCGCGGCCAGGCTGCGTGAGGCAACGCCCAGCCGGTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTTTTCGGGGTTg < 3:2006/136‑1 (MQ=255) cTGGCCATCGACGCCAGCAAGTAGCCGCCCAGCAGCGCGGCCAGGCTGCGTGAGGCAACGCCCAGCCGGTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTTTTCGGGGTTGCaa > 4:160725/1‑135 (MQ=255) cTGGCCATCGACGCCAGCAAGTAGCCGCCCAGCAGCGCGGCCAGGCTGCGTGAGGCAACGCCCAGCCGGTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTTTTCGGGGTTGCa > 6:363247/1‑134 (MQ=255) cTGGCCATCGACGCCAGCAAGTAGCCGCCCAGCAGCGCGGCCAGGCTGCGTGAGGCAACGCCCAGCCGGTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTGTTCGGGGTTGCa > 2:38325/1‑134 (MQ=255) cTGGCCATCGACGCCAGCAAGTAGCCGCCCAGCAGCGCGGCCAGGCTGCGTGAGGCAACGCCCAGCCGGTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTGTTCGGGGGTGcg > 1:129663/1‑133 (MQ=255) ggCTATCGACGCCAGCAAGTAGCCGCCCAGCAGCGCGGCCAGGCTGCGTGAGGCAACGCCCAGCCGGTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTTTTCGGGGTTGCAATa > 6:79208/1‑135 (MQ=255) ggCCATCGACGCCAGCAAGTAGCCGCCCAGCAGCGCGGCCAGGCTGCGTGAGGCAACGCCCAGCCGGTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTTTTCGGGGTTGCAATa > 6:302593/1‑135 (MQ=255) ccATCGACGCCAGCAAGTAGCCGCCCAGCAGCGCGGCCAGGCTGCGTGAGGCAACGCCCAGCCGGTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTTTTCGGGGTTGCAATa < 2:31384/133‑1 (MQ=255) cATCGACGCCAGCAAGTAGCCGCCCAGCAGCGCGGCCAGGCTGCGTGAGGCAACGCCCAGCCGGTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTTTTCGGGGTTGCAATACTaa > 4:263599/1‑136 (MQ=255) cATCGACGCCAGCAAGTAGCCGCCCAGCAGCGCGGCCAGGCTGCGTGAGGCAACGCCCAGCCGGTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTTTTCGGGGTTGCAATACTaa > 8:196903/1‑136 (MQ=255) cATCGACGCCAGCAAGTAGCCGCCCAGCAGCGCGGCCAGGCTGCGTGAGGCAACGCCCAGCCGGTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTTTTCGGGGTTGCAATACTa > 1:117352/1‑135 (MQ=255) aTCGACGCCAGCAAGTAGCCGCCCAGCAGCGCGTCCAGGCTGAGTGAGGCAACGCCCAGCCGGTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTTTTCGGGGTAGCAATACTAAt > 4:161910/1‑136 (MQ=255) aTCGACGCCAGCAAGTAGCCGCCCAGCAGCGCGGCCAGGCTGCGTGAGGCAACGCCCAGCCGGTAACTGAACTGGAGGCCGGCGGTTTTGAGCGTCATGGCGAAGGTCCGGGCGTTTTCGGGGTTGCAATACTAAt > 3:337864/1‑136 (MQ=255) aTCGACGCCAGCAAGTAGCCGCCCAGCAGCGCGGCCAGGCTGCGTGAGGCAACGCCCAGCCGGTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTTTTCGGGGTTGCAATACTaa > 6:325342/1‑135 (MQ=255) aTCGACGCCAGCAAGTAGCCGCCCAGCAGCGCGGCCAGGCTGCGTGAGGCAACGCCCAGCCGGTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTTTTCGGGGTTGCAATACTAAt > 4:98027/1‑136 (MQ=255) aTCGACGCCAGCAAGTAGCCGCCCAGCAGCGCGGCCAGGCTGCGTGAGGCAACGCCCAGCCGGTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTGTTCGGGGTTGCAATACTaa > 1:232922/1‑135 (MQ=255) aTCGACGCCAGCAAGTAGCCGCCCAGCAGCGCGGCCAGGCTGCGTGAGGCAACGCCCAGCCGCTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTGTTGGGGGTGGCAAGACTaa > 5:102783/1‑135 (MQ=255) aCGCCAGCAAGTAGCCGCCCAGCAGCGCGGCCAGGCTGCGTGAGGCAACGCCCAGCCGGTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGGTTTCGGGGGTGCAATACTAATGAtt > 4:120247/1‑136 (MQ=255) ccAGCAAGTAGCCGCCCAGCAGCGCGGCCAGGCTGCGTGAGGCAACGCCCAGCCGGTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTTTTCGGGGTTGCAATACTAATGATTTCa < 7:113290/136‑1 (MQ=255) aaGTAGCCGCCCAGCAGCGCGGCCAGGCTGCGTGAGGCAACGCCCAGCCGGTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTTTTCGGGGTTGCAATACTAATGATTTCAATtc < 5:79208/135‑1 (MQ=255) aaGTAGCCGCCCAGCAGCGCGGCCAGGCTGCGTGAGGCAACGCCCAGCCGGTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTTTTCGGGGTTGCAATACTAATGATTTCAATtc < 1:43031/135‑1 (MQ=255) ccgccCAGCAGCGCGGCCAGGCTGCGTGAGGCAACGCCCAGCCGGTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTTTTCGGGGTTGCAATACTAATGATTTCAATTCTCATaa < 1:101487/135‑1 (MQ=255) cccAGCAGCGCGGCCAGGCTGCGTGAGGCAACGCCCAGCCGGTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTTTTCGGGGTTGCAATACTAATGATTTCAATTCTCATAAGCa > 4:173695/1‑135 (MQ=255) ccAGCAGCGCGGCCAGGCTGCGTGAGGCAACGCCCAGCCGGTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTTTTCGGGGTTGCAATACTAATGATTTCAATTCTCATAAGCaa < 5:312410/135‑1 (MQ=255) cgGCCAGGCTGCGTGAGGCAACGCCCAGCCGGTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTTTTCGGGGTTGCAATACTAATGATTTCAATTCTCATAAGCAAAGCCTGCTGa < 4:344593/136‑1 (MQ=255) ccAGGCTGCGTGAGGCAACGCCCAGCCGGTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTTTTCGGGGTTGGAATACTAATGATTTCAATTCTCATAAGCAAAGCCTGCTGCCAg > 8:138475/1‑136 (MQ=255) gTGAGGCAACGCCCAGCCGGTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTTTTCGGGGTTGCAATACTAATGATTTGAATTCTCATAAGCAAAGCCTGCTGACAGGTGACGa < 1:132795/134‑1 (MQ=255) tGAGGCAACGCCCAGCCGGTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTTTTCGGGGTTGCAATACTAATGATTTCAATTCTCATAAGCAAAGCCTGCTGACAGGGGACGATTg < 8:272642/136‑1 (MQ=255) gAGGCAACGCCCAGCCGGTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTTTTCGGGGGTGCAATTCGAATGAGTTGAATTATGATAAGCAACGCCGGCGTAAAGGTGCAGAtttc > 3:257344/1‑134 (MQ=255) aaCGCCCAGCCGGTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTGGTCGGGGGTGGAATACTAATGATTTCAATTCTCATAAGCAAAGCCTGCTGCCAGGTGACGATTGCCGaaa > 3:16224/1‑136 (MQ=255) gCCCAGCCGGTAACTGTACGGGAGGCCGGCGGTTTTGGGCGTCATGGAGTAGGTCCTGGCGTTTTCGGGGTTTCAATACTAATGATTTCAATTCTCATAAGCAAAGCCTGGTGACAGGTGACGATTGCCGAAACAg < 8:50044/136‑1 (MQ=255) gCCCAGCCGGTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTTTTCGGGGTTGCAATACTAATGATTTCAATTCTCATAAGCAAAGCCTGCTGACAGGTGACGATTGCCGAAACAg < 3:98027/136‑1 (MQ=255) cccAGCCGGTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTTTTCGGGGTTGCAATACTAATGATTTCAATTCTCATAAGCAAAGCCTGCTGACAGGTGACGATTGCCGAAACAg < 1:70333/135‑1 (MQ=255) cccAGCCGGTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTTTTCGGGGTTGCAATACTAATGATTTCAATTCTCATAAGCAAAGCCTGCTGACAGGTGACGATTGCCGAAACAg < 5:363247/135‑1 (MQ=255) cccAGCCGGTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTTTTCGGGGTTGCAATACTAATGATTTCAATTCTCATAAGCAAAGCCTGCTGACAGGTGACGATTGCCGAAACAg < 5:97975/135‑1 (MQ=255) ccAGCCTGTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTTTTCGGGGTTGCAATACTAATGATTTCAATTCTCATAAGCAAAGCCTGCTGACAGGTGACGATTGCCGAAACAGAc < 4:337864/136‑1 (MQ=255) ccAGCCGGTAAGTGAAGGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGGGTTTTGGGGGTTGCAATACTAATGATTTCTATTCTCATAAGCAAAGCCTGCTGACAGGTGACGATTGCCGAAACTGAc < 8:390237/136‑1 (MQ=255) ccAGCCGGTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTTTTCGGGGTTGCAATACTAATGATTTCAATTCTCATAAGCAAAGCCTGCTGACAGGTGACGATTGCCGAAACAGAc < 7:196903/136‑1 (MQ=255) ccAGCCGGTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTTTTCGGGGTTGCAATACTAATGATTTCAATTCTCATAAGCAAAGCCTGCTGACAGGTGACGATTGCCGAAACAGAc < 7:26254/136‑1 (MQ=255) cAGCCGGTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTTTTCGGGGTTGCAATACTAATGATTTCAATTCTCATAAGCAAAGCCTGCTGACAGGTGACGATTGCCGAAACAGAc < 1:208960/135‑1 (MQ=255) ggTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTTTTCGGGGTTGCAATACTAATGATTTCAATTCTCATAAGCAAAGCCTGCTGACAGGTGACGATTGCCGTAACAGACGGCCAc > 8:95622/1‑136 (MQ=255) cTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTGTTAGGGGTTTGAATACTAATGATTTCAATTATCAAAAGGAAATCCGGCTGACAGGTGTCGATTGGCGAAACAGACGGCCCCCCaaa > 7:388116/1‑134 (MQ=255) gaccGGGAGGCCGGCGGTTTTGCGCGTCATGGAGAAGGGCCGGGCGTTTTCGGGGGTGCAATACTAATGATTTCAATTCTCATAAGCAAAGCCTTCTGACAGGTGACGATTGCCGAAACAGACGGCCACCCATAc < 1:323429/132‑1 (MQ=255) aaCGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTTTTCGGGGTTGCAATACTAATGATTTCAATTCTCATAAGCAAAGCCTGCTGACAGGTGACGATTGCCGAAACAGACGGCCACCCATAc < 4:380914/134‑1 (MQ=255) aCGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGGCCGGGCGTTTTCGGGGTTGCAATACTAATGATTTCAATTCTCATAAGCAAAGCCTGCTGACAGGTGACGATTGCCGAAACAGACGGCCACCCATAc < 5:229041/133‑1 (MQ=255) ggcggTTTTGCGCGTCATGGCGAAGGTACGGGCGTTTTAGGGGTTGCAATAATAATGATTTCAATTCTCATAAGCAAAGCCTGCTGACAGGTGACGATTGCCGAAACGGACGGCCACCCATACAATGCGAACaa > 6:287519/1‑134 (MQ=255) ggTTTTGCGCGTCATGGCGAAGGTCCGGGCGTTTTCGGGGTTGCAATACTAATGATTTCAATTCTCATAAGCAAAGCCTGCTGACAGGTGACGATTGCCGAAACAGACGGCCACCCATACAATGCGAACAATTCtt < 3:134720/136‑1 (MQ=255) ggTTTTGCGCGTCATGGCGAAGGTCCGGGCGTTTTCGGGGTTGCAATACTAATGATTTCAATTCTCATAAGCAAAGCCTGCTGACAGGTGACGATTGCCGAAACAGACGGCCACCCATACAATGCGAACAATTCtt < 8:26216/136‑1 (MQ=255) cgTCATGGCGAAGGTGCGGGCGTTTTCGGGGTTGCAATACTAATGATTTCAATTCTCATAAGCAAAGCCTGCTGACAGGTGACGATTGCCGAAACAGACGGCCACCCATACAATGCGAACAATTCTTACTACCAg < 6:102783/135‑1 (MQ=255) tCATGGCGAAGGTCCGGGCGTTTTCGGGGTTGCAATACTAATGATTTCAATTCTCATAAGCAAAGCCTGCTGACAGGTGACGATTGCCGAAACAGACGGCCACCCATACAATGCGAACAATTCTTACTACCAGtt < 6:135204/135‑1 (MQ=255) aGGGCCGGGCGTTTTCGGGGTTGCAATACTAATGATTTCAATTCTCATAAGCAAAGCCTGCTGACAGGTGACGATTGCCGAAACAGACGGCCACCCATACAATGCGAACAATTCTTACTACCAGTTGCGCTGTCAt < 3:144183/136‑1 (MQ=255) tCCGGGCGTTTTCGGGGTTGCAATACTAATGATTTCAATTCTCATAAGCAAAGCCTGCTGACAGGTGACGATTGCCGAAACAGACGGCCACCCATACAATGCGAACAATTCTTACTACCAGTTGCGCTGTCATgcg < 3:263599/136‑1 (MQ=255) tCCGGGCGTTTTCGGGGTTGCAATACTAATGATTTCAATTCTCATAAGCAAAGCCTGCTGACAGGTGACGATTGCCGAAACAGACGGCCACCCATACAATGCGAACAATTCTTACTACCAGTTGCGCTGTCATgcg > 8:140329/1‑136 (MQ=255) gccGGGCGTTTTCGGGGGTGCAATACTAATGATTTCAATTCTCATAAGCAAAGCCTGCTGACAGGGGACGATTGCCGAAACAGACGGCCACCCATACAATGCGAACAATTCTTACTACCAGTTGCGCTGTCATgcg < 4:16224/135‑1 (MQ=255) ccGGGCGTTTTCGGGGTTGCAATACTAATGATTTCAATTCTCATACGCAAAGCCTGCTGACAGGTGACGATTGCCGAAACAGACGGCCACCCATACAATGCGAACAATTCTTACTACCAGTTGCGCTGTCATgcg < 1:369205/135‑1 (MQ=255) ccGGGCGTTTTCGGGGTTGCAATACTAATGATTTCAATTCTCATAAGCAAAGCCTGCTGACAGGTGACGATTGCCGAAACAGACGGCCACCCATACAATGCGAACAATTCTTACTACCAGTTGCGCTGTCATgcg < 2:232922/135‑1 (MQ=255) gggCGTTTTCGGGGTTGCAATACTAATGATTTCAATTCTCATAAGCAAAGCCTGCTGACAGGTGACGATTGCCGAAACAGACGGCCACCCATACAATGCGAACAATTCTTACTACCAGTTGCGCTGTCATGCGcc < 6:84838/135‑1 (MQ=255) tCGGGGTTGCAATACTAATGATTTCAATTCTCATAAGCAAAGCCTGCTGACAGGTGACGATTGCCGAAACAGACGGCCACCCATACAATGCGAACAATTCTTACTACCAGTTGCGCTGTCATGCGCCGGAGTATc < 7:15602/135‑1 (MQ=255) cGGGGGTGCAATACGAAAGATTTCAATTCTCATAAGCAAAGCCTGCTGACAGGGGACGCTTGCCGCAACAGACGGCCACCCATACAAAGCGAACAATTCTTACTACCCGTTGCGCTGTCATGCGCCGGAGTATCa > 2:295728/1‑135 (MQ=255) | GACACTGGCCATCGACGCCAGCAAGTAGCCGCCCAGCAGCGCGGCCAGGCTGCGTGAGGCAACGCCCAGCCGGTAACTGAACGGGAGGCCGGCGGTTTTGCGCGTCATGGCGAAGGTCCGGGCGTTTTCGGGGTTGCAATACTAATGATTTCAATTCTCATAAGCAAAGCCTGCTGACAGGTGACGATTGCCGAAACAGACGGCCACCCATACAATGCGAACAATTCTTACTACCAGTTGCGCTGTCATGCGCCGGAGTATCA > ACB122/5226858‑5227120 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |