Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 5,358,465 T→G 22.4% E46A (GAG→GCG)  folP ← Dihydropteroate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,358,4650TG22.4% 120.6 / 2.3 79E46A (GAG→GCG) folPDihydropteroate synthase
Reads supporting (aligned to +/- strand):  ref base T (23/32);  new base G (4/17);  total (29/50)
Fisher's exact test for biased strand distribution p-value = 1.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCT  >  ACB122/5358331‑5358598
                                                                                                                                      |                                                                                                                                     
ccTCGACCATGGGCGCCACACGCCCCAGCTCCGCGGGCCCCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCt                                                                                                                                       <  1:110249/135‑1 (MQ=255)
    gACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGGGGGCTCGCCACCGCTATAGCTCAACCTTGCGCCGGCGGCCACCAAAGCTTCGGCGTGGCGCCGGGCCtcctcg                                                                                                                                  >  8:348376/1‑136 (MQ=255)
    gACCATTGCCCCCCCACGCCCCCGCCCCTCGGCCCCCGAGACTGCCCGCCCCCCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCtcgtc                                                                                                                                   <  5:300863/135‑1 (MQ=255)
      ccATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGGCTCGCCACCCATATAGCTCAACCTTGCGCCGGCCGCAACCCTCGCTTCGGCGGGGCGCACGGGCTCGTcgc                                                                                                                                 >  6:108642/1‑135 (MQ=255)
      ccATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGGGGACTCCCCCCCGAAAAAGATCAAACTTGCGCCGGCGGCCCCCCACGCGTAGGCGTGGCGCCGGGCCACCTcgcg                                                                                                                                >  4:150670/1‑136 (MQ=255)
             cgcCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGGGGGCGCGCCCCCGCTATCGCTCAACCTTGCGCCCGCGGCCACCCTCCCGTCGGCGGGGCGCCGGGCCGCGgcgcgcggccg                                                                                                                          >  5:310809/1‑130 (MQ=255)
             cgcCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGGGGCCTCGCCCCCGCTATCGCTCAACGTTGCGCCGGCGGCCACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGcggac                                                                                                                           >  1:46695/1‑134 (MQ=255)
              gcCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGAGATAGATCAACCTTTCGCCGGCGGCAACCAACGCTTCGGCGTGGCGCAGGGCCTCGTCgcgcggccg                                                                                                                          >  2:38265/1‑129 (MQ=255)
                 ccacGCTCCAGCGCCTCGGTCACCGACACTGCCCGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAg                                                                                                                      <  2:95517/134‑1 (MQ=255)
                  cacGCTCCAGCTCCTCGGGCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCTATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGAAGGGCCTCGTCGCGCTGACTGAAGc                                                                                                                     <  6:152132/135‑1 (MQ=255)
                          aGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGGCGCGCCCCCGCTATCGCTCAACGTTGCGCCGGCGGCCACCACAGCGTCGGCGTGGCGCCGGGCCGCGGCGCGCGGGCTGCAGCGgccgccct                                                                                                            >  4:317594/1‑134 (MQ=255)
                             tcctcGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGTCTAGCCACCCCTATAGATAAACCTTGCGCCCGCGGCAACCATCGCTTACGCCTTGCGGAGGGCCTCGACGCGCGGACTGAATCGACCGCCATc                                                                                                           >  6:176429/1‑134 (MQ=255)
                                    tCACCGAGACTGCACGCGCACCCGGGCGCGTGGGCTCGCCCCCGATATCGATCAACGTTGCGCCGGCGGCCACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCGGGCTGAAGCGCCCGCCCACGGAGCAgg                                                                                                   >  4:325744/1‑135 (MQ=255)
                                    tCACCGAGACTGCACGCGCACCCGGGCGCGTGGGCTCGCCACCGATATCGATCAAACGTGCGCCCGCGGCAACCCAAGCGTAGGCGTGGCGCACGGCCACGGCGCGCGGACGGCAGCGCCCCCCCTCGGAGAAgg                                                                                                   >  4:314682/1‑135 (MQ=255)
                                    tCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCCCCGATATCGCTCAAAGTTGCGCCCGCGGCAACCAACGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCGGACTGAAGCGGCCGCCCTCCGAGAAg                                                                                                    >  5:178834/1‑134 (MQ=255)
                                    tCACCGAGACTGCACGCGCACCCGGGCGCGGGGCCTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCCACCCTCGCGTCGGCGTGGCGCAGGGCCGCGTCGCGCGGGCGGAAGCGCCCCCCAGCGGCGAAg                                                                                                    >  5:204004/1‑134 (MQ=255)
                                    gcACCGAGACTGCCGGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGAGCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGa                                                                                                  <  4:300089/135‑1 (MQ=255)
                                      aCCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAAt                                                                                                <  4:184839/136‑1 (MQ=255)
                                         gagaCTGCACGCGCACCCGGGCGCGTGGGCTCGCCCCCGCTATAGATCAAACTTTCGCCGGCGGCCACCCACGCGTCGGCGGGGGGCCGGGGCGCGTCGCGCGGACTGAAGCGCCCGCCCTCGGGGAAGGAATCg                                                                                              >  2:313632/1‑135 (MQ=255)
                                             cggcccgcgCACCGGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGt                                                                                         <  7:191340/131‑1 (MQ=255)
                                               gCAGGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTg                                                                                        <  2:2352/135‑1 (MQ=255)
                                                   gcgcACCCGGGCGCGTGGTCTCGCCCCCGATATAGATCAACGTTGCGCCGGCGGCAACCATCGCGTCGGCGTGGGGCAGGGCCTCGTGGCGCGGGCTGCAGCGACCGCCCTCGGAGAAGGAATCGGGGGTGATAtt                                                                                   >  8:365366/1‑136 (MQ=255)
                                                   gcgcACCCGGGCGCGTGGTCTCGCCCCCGAAATAGATCAACGTTGCGCCGGCGGCCACCATCGCTTAGGCGTGGCGCCGGGCCTCGTCGGGCGGGCAGTAGCGCCCCCCATCCGTGAAGGCATCgggggggggat                                                                                    >  2:273484/1‑129 (MQ=255)
                                                     gcACCCGGGCGCGTGGGCGCGGCACCGCTATCGCTCAACGTTGCGCCGGCGGCCCCCATCGCGTCGGCGTGGGGCCGGGCCTCGTCGCGCGGGCTGAAGCGGCCGCCCGCGGTGAAGGAATCGGGGGGGatat                                                                                    >  2:51466/1‑133 (MQ=255)
                                                     gcACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTga                                                                                 <  4:100618/136‑1 (MQ=255)
                                                         ccGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCTCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGTGAAt                                                                             <  3:301071/136‑1 (MQ=255)
                                                         ccGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAAt                                                                             <  3:164457/136‑1 (MQ=255)
                                                         ccGGGCGAGTGGACTCGCCACCGATATCGAGCAAGGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGACTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAAt                                                                             <  7:99251/136‑1 (MQ=255)
                                                         ccGGCCGCGTGGACTCGCCACCGATCTCGATCACCGTTGCGCCGGCGGCAACCAGCGCTTCGGGGTGGCGCAGGGCCGCGTCGCGCTGACTGACGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAAt                                                                             <  3:53489/136‑1 (MQ=255)
                                                          gggccgcgTGGACTCGCCACCGATATCGATCACCTTTGCGCGGGGGGTAACCTTCGCTTCGGCGTGGCGCAGGGCGTGGGCGGGATGACTCAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAAt                                                                             <  5:274445/131‑1 (MQ=255)
                                                           gggCGGGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCAGCGCTTCGGCGTGGCGCAGGGCCGCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATa                                                                            <  6:357871/135‑1 (MQ=255)
                                                           gggCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATAc                                                                           <  3:68946/136‑1 (MQ=255)
                                                               gcgTGGACTCGCCACGGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCAt                                                                       <  7:252012/136‑1 (MQ=255)
                                                                cggggACTCGCCACCGATATCGATCACCGTGGCGCCGGCGGCAACCAGCGCTGCGGCGGGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCAt                                                                       <  5:310648/132‑1 (MQ=255)
                                                                 gTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCGACGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGa                                                                     <  3:157796/136‑1 (MQ=255)
                                                                 gTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATg                                                                      <  2:208783/135‑1 (MQ=255)
                                                                  tGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTGCGGCGGGGCGCAGGGCCGCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGa                                                                     <  1:207573/135‑1 (MQ=255)
                                                                  gggACTCGCCACCGATAGCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCGCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGa                                                                     <  2:302569/134‑1 (MQ=255)
                                                                            aCCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGCCTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGc                                                          >  3:14868/1‑136 (MQ=255)
                                                                            aCCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCgggg                                                                                           >  1:140032/1‑103 (MQ=255)
                                                                            aCCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCgggg                                                                                           <  2:140032/103‑1 (MQ=255)
                                                                            aCCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGc                                                          >  3:356203/1‑136 (MQ=255)
                                                                             ccGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGc                                                          <  1:18876/135‑1 (MQ=255)
                                                                                 tatCGATCACCGTTGCGCCGGCGGAAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGCAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACaa                                                     <  3:314682/136‑1 (MQ=255)
                                                                                    cGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACaaag                                                   >  7:245862/1‑134 (MQ=255)
                                                                                              tGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGCCCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCg                                         >  1:269076/1‑135 (MQ=255)
                                                                                              tGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAAt                                                                             >  2:376095/1‑99 (MQ=255)
                                                                                              tGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAAt                                                                             <  1:376095/99‑1 (MQ=255)
                                                                                              tGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCg                                         >  1:212791/1‑135 (MQ=255)
                                                                                                   cggcggCAACCATCGCTT‑GGCGTGGCGCAGGGCCGCGGCGCGCTGACTGACGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCg                                  <  8:163435/136‑1 (MQ=255)
                                                                                                        gcACCCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGATCCCGGCTGCCGCaa                               >  5:396515/1‑135 (MQ=255)
                                                                                                        gcAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAg                              >  7:371453/1‑136 (MQ=255)
                                                                                                        gcAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAg                              >  7:381603/1‑136 (MQ=255)
                                                                                                        gcAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACAGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAg                              >  4:374458/1‑136 (MQ=255)
                                                                                                         cAACCATCGCTTGGGCGTGGCGCAGGGCCGCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGCGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAgg                             <  4:122382/136‑1 (MQ=255)
                                                                                                         cAACCATCGCTTCGGCGGGGCGCAGGGCCGCGTCGCGCTGACTTAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAgg                             <  8:371453/136‑1 (MQ=255)
                                                                                                         cAACCAGCGCTGCGGGGTGGCGCAGGGCCTCGGCGCGCTGACTGACGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAgg                             <  7:348376/136‑1 (MQ=255)
                                                                                                          aaCCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAgg                             <  6:178834/135‑1 (MQ=255)
                                                                                                          aaCCATCGCTTCGGCGTGGCGCAGGGCCGCGGCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAgg                             <  6:98752/135‑1 (MQ=255)
                                                                                                          aaCCATCGCTTCGGCGGGGCGCCGGGCCGCGTCGCGCGGACTTCCGCGACCGCCATCGGAGAAGGACTCGGGGGGGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAgg                             <  6:377591/135‑1 (MQ=255)
                                                                                                          aaCCATCGCTGGGGCGGGGCGCTGGGCCGCGGCGCGGGGACTGACGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAgg                             <  5:72814/135‑1 (MQ=255)
                                                                                                            ccAGCGCTGCGGCGGGGCGCTGGCCCGCCCCGCGCTGACTCACGCGACCGCCATCGGAGAAGGAATCGGGGGGGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCa                           <  5:315534/135‑1 (MQ=255)
                                                                                                              aTCGCTTCGGCGTGGCGCAGGGCCTCGTGGCGGTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAAcc                        <  3:175623/136‑1 (MQ=255)
                                                                                                                cgcgTCGGCGGGGCGCAGGGCCGCGGCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCgg                      <  3:116418/136‑1 (MQ=255)
                                                                                                                cGCTGCGGCGTGGCGCAGGGCCGCGGCGCGCGGACTGACGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAAGCAAGAACCCGGCTGCCGCAAGGCAACCgg                      <  7:104664/136‑1 (MQ=255)
                                                                                                                 gCTTGGGCGGGCCCCCGGGCCGCGGCCCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCgg                      <  6:265024/135‑1 (MQ=255)
                                                                                                                 gCTTCGGCGTGGCGCAGGGCCTCGGCGCGCTGACTCACGCGCACGCCAGCGGAGAAGGCATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCgg                      <  6:262678/135‑1 (MQ=255)
                                                                                                                  ctgcGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCAGCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCgg                      <  6:204004/131‑1 (MQ=255)
                                                                                                                  cTTCGGGGGGGCGCAGGGCCTCGTCGCGCTGACTGACGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCgg                      <  5:176429/134‑1 (MQ=255)
                                                                                                                  cTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCCTAGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACAGGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGCCACACgggt                    >  7:371443/1‑136 (MQ=255)
                                                                                                                    tgggCGGGGCGCAGGGCCGCGTCGCGCGTACGGACGCGACCGCCATCGGAGAAGGAAGCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCgggtag                  <  7:393116/134‑1 (MQ=255)
                                                                                                                      ggCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCgggtagg                 <  8:381603/135‑1 (MQ=255)
                                                                                                                           ggCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACt            <  2:46695/135‑1 (MQ=255)
                                                                                                                           ggCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTg           <  4:178109/136‑1 (MQ=255)
                                                                                                                            gcgcTGGGCGTCGTCGCGCGTACTGACGGGACGGCCATCGGAGTAGCAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATGAAGAACCCGGCTGCCTCAAGGCAACCGGGTAGGGTACTGc          <  7:196317/136‑1 (MQ=255)
                                                                                                                            gcgcAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTg           <  6:310809/135‑1 (MQ=255)
                                                                                                                             cgcAGGGCCGCGTCGCGCTCACTCACGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGc          <  5:190436/135‑1 (MQ=255)
                                                                                                                                aGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTg      <  8:113535/136‑1 (MQ=255)
                                                                                                                                 gggCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGa     <  7:365366/136‑1 (MQ=255)
                                                                                                                                 gggCCGCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGa     <  3:34669/136‑1 (MQ=255)
                                                                                                                                     cTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGc   >  2:336574/1‑134 (MQ=255)
                                                                                                                                     cTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCt  >  7:103628/1‑135 (MQ=255)
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CCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCT  >  ACB122/5358331‑5358598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑