Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB122	179,484	T→C	C316R (TGC→CGC)	CJ019_00182 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	179,484	0	T	C	100.0%	195.7 / NA	62	C316R (TGC→CGC)	CJ019_00182	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/34);  total (28/34)

TTCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCTGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGT  >  ACB122/179359‑179614                                                                                                                              |                                                                                                                                   ttCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCcgcg                                                                                                                           >  1:19354/1‑135 (MQ=255)  tCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTCTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCgcgg                                                                                                                          <  8:183454/135‑1 (MQ=255)    aGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAACCCAACGACCTCAGTTCCCGCAGCCgcggcg                                                                                                                        >  3:85994/1‑135 (MQ=255)           ggctggcCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGtt                                                                                                                 <  7:87662/135‑1 (MQ=255)            gctggcCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGGTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGttt                                                                                                                <  4:108200/135‑1 (MQ=255)             ctggcCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGttt                                                                                                                >  7:239136/1‑134 (MQ=255)             ctggcCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGAAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTg                                                                                                               <  6:329016/135‑1 (MQ=255)              tggcCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCgcggcggcgcggtt                                                                                                                >  5:265538/1‑128 (MQ=255)                   tGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGc                                                                                                         >  2:49225/1‑135 (MQ=255)                        gcccgccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCa                                                                                                    >  1:92900/1‑135 (MQ=255)                            gccTTGGCGGCGAGCGTACCGAGCAGGTGGTCCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTAGCAGTGCGGGCACCgg                                                                                                <  6:17912/135‑1 (MQ=255)                            gccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                <  1:305918/135‑1 (MQ=255)                            gccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                <  2:92900/135‑1 (MQ=255)                             ccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                >  4:49174/1‑134 (MQ=255)                              cTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                <  7:27494/133‑1 (MQ=255)                              cTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                >  8:27494/1‑133 (MQ=255)                              cTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGc                                                                                              <  2:19354/135‑1 (MQ=255)                                        aGCGTACCGACCAGTTGTTGGAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACAAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATg                                                                                    <  5:253142/135‑1 (MQ=255)                                        aGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATg                                                                                    <  4:99791/135‑1 (MQ=255)                                        aGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATg                                                                                    <  7:304736/135‑1 (MQ=255)                                           gTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATga                                                                                   >  8:22351/1‑133 (MQ=255)                                           gTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATga                                                                                   >  6:387355/1‑133 (MQ=255)                                               cGACCAGTTGTTGCAAGGGGTAGGTGATCAGCTGCGTCGTACTTGTGCCTGCTACCCGGAAACCCACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGc                                                                             <  5:199974/135‑1 (MQ=255)                                                    aGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTac                                                                        >  1:62714/1‑135 (MQ=255)                                                         ttgCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCggg                                                                                               <  6:8910/107‑1 (MQ=255)                                                         ttgCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgcg                                                                                               >  5:8910/1‑105 (MQ=255)                                                         ttgCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCtt                                                                   >  4:137365/1‑135 (MQ=255)                                                          tgCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTg                                                                  <  2:4771/135‑1 (MQ=255)                                                          tgCAAGCGGGAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTg                                                                  <  4:77488/135‑1 (MQ=255)                                                              aGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCa                                                              >  3:131498/1‑135 (MQ=255)                                                               gCGGGAGGTGAACAGCTGCGTCGTACTTGTGACAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAg                                                             <  4:82308/135‑1 (MQ=255)                                                                cGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAgg                                                            >  4:304197/1‑135 (MQ=255)                                                                 ggTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCgcgg                                                                                                                          <  5:185586/72‑1 (MQ=255)                                                                 ggTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCgcgg                                                                                                                          >  6:185586/1‑72 (MQ=255)                                                                      gTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTgg                                                      <  1:49225/135‑1 (MQ=255)                                                                           cAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGTGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCa                                                 <  4:232483/135‑1 (MQ=255)                                                                                gCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGtt                                                                                                                 <  6:238474/66‑1 (MQ=255)                                                                                gCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGtt                                                                                                                 >  5:238474/1‑66 (MQ=255)                                                                                gCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGtt                                            >  8:304838/1‑135 (MQ=255)                                                                                         ttGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCag                                        >  5:127121/1‑130 (MQ=255)                                                                                         ttGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCag                                        <  6:127121/130‑1 (MQ=255)                                                                                           gtgCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCAcc                                                                                                  <  1:355559/70‑1 (MQ=255)                                                                                           gtgCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCAcc                                                                                                  >  2:355559/1‑70 (MQ=255)                                                                                              ccAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCAc                               >  2:212757/1‑134 (MQ=255)                                                                                                aGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGaa                            <  2:211050/135‑1 (MQ=255)                                                                                                   tACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACg                         >  1:264866/1‑135 (MQ=255)                                                                                                   tACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACg                         <  1:326329/135‑1 (MQ=255)                                                                                                   tACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACg                         >  6:84768/1‑135 (MQ=255)                                                                                                   tACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACg                         >  8:174557/1‑135 (MQ=255)                                                                                                   tACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACg                         >  7:387416/1‑135 (MQ=255)                                                                                                    aCCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACCCCTTGCCCAGGCTCGGGAGGCAACGTTGCAGAGCCTGCACGCAACgg                        >  3:6087/1‑135 (MQ=255)                                                                                                        ggAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCggg                                                                                               >  5:190418/1‑60 (MQ=255)                                                                                                        ggAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCggg                                                                                               <  6:190418/60‑1 (MQ=255)                                                                                                                   aCCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATg                                                                                    <  3:43807/60‑1 (MQ=255)                                                                                                                   aCCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATg                                                                                    >  4:43807/1‑60 (MQ=255)                                                                                                                     cTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGAc       <  4:131498/135‑1 (MQ=255)                                                                                                                          ttCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATTAGGAGGCTGTAAACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGt  <  3:49174/135‑1 (MQ=255)                                                                                                                          ttCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGt  <  3:304197/135‑1 (MQ=255)                                                                                                                          ttCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGt  <  7:234477/135‑1 (MQ=255)                                                                                                                          ttCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGt  <  7:153945/135‑1 (MQ=255)                                                                                                                          ttCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGt  <  6:265538/135‑1 (MQ=255)                                                                                                                          ttCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGt  <  8:239136/135‑1 (MQ=255)                                                                                                                              |                                                                                                                                   TTCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCTGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGT  >  ACB122/179359‑179614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑