Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 5,756,055 Δ1 bp 100% coding (776/834 nt) CJ019_05400 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,756,0550A.89.0% 136.6 / 3.9 36coding (776/834 nt)CJ019_05400hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  major base . (26/6);  minor base T (0/2);  total (26/10)
Fisher's exact test for biased strand distribution p-value = 4.99e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGCTGCAAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGC  >  ACB122/5755923‑5756183
                                                                                                                                    |                                                                                                                                
aGCTGCAAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGaa                                                                                                                                 >  5:93457/1‑134 (MQ=255)
        gcgcgcGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAAcgccg                                                                                                                        >  1:246696/1‑134 (MQ=255)
              gAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCcagca                                                                                                                  >  3:175235/1‑134 (MQ=255)
               aaCAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAACCCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAgg                                                                                                                >  5:4207/1‑135 (MQ=255)
                              aCCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGCGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGgccgccg                                                                                                 <  7:32606/135‑1 (MQ=255)
                                 tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGccgccgccg                                                                                              >  7:176146/1‑135 (MQ=255)
                                 tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCAGGCGCAGCAGGACGCGGAAAAACGCCGCCAGCAGGAAGCGCTGgccgccgcc                                                                                               >  1:212442/1‑134 (MQ=255)
                                          tCTGGACGACCATCGCGCAACCCCCCCCCCGCCCGGCCGGTGAAGCTCACCAGCCACGCAACACGGCCCTGGGGGCGCAGCAGTAAGGGAAAAAACGCCGCCAGCAGGAAGCGCGGGCCGCCGCCGCTGCCcgcg                                                                                     <  8:117198/135‑1 (MQ=255)
                                                aGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCggg                                                                               >  8:16407/1‑135 (MQ=255)
                                                aGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCggg                                                                               >  5:275350/1‑135 (MQ=255)
                                                 caCCATCGCGCACACCCCCGCCCCCCGGCCCCGTGACGCTGACCCGCCCCCCAACACGGCCCTGCTGGCGCCGCCGGAACCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGc                                                                              <  1:32666/134‑1 (MQ=255)
                                                    cagcGAGGAAACCCTCGCCCGCCCGGCCCGTGACGCTGAAGAGGCACGCAAGAAGGCCCTGCGGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCg                                                                           <  8:86723/132‑1 (MQ=255)
                                                       cGAGGAAACCCTCGCCCGCCAGGCCCGTGCAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCATGAAGCGGAACGCCGCCGCACGCAGGCAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGa                                                                        >  1:115910/1‑135 (MQ=255)
                                                       cGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGa                                                                        >  3:116731/1‑135 (MQ=255)
                                                       cGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGa                                                                        >  5:116900/1‑135 (MQ=255)
                                                         tgcaacccccCGCCCGCCGGGCCCTTAACGGTGAACTGCCACCCACGATGGCCCTGGGGGGCCAGCTGTAAGCGTAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGc                                                                      <  6:64631/129‑1 (MQ=255)
                                                         agcaaccccGCGCCCGCCAGGCCGGTGACGCTCAAGAGCCACGCAAGACGGCCGTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGc                                                                      <  3:146612/129‑1 (MQ=255)
                                                         aGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGc                                                                      >  5:271300/1‑135 (MQ=255)
                                                         aGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGc                                                                      >  2:189026/1‑135 (MQ=255)
                                                         aGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTCGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCCGGCCCGTGAGc                                                                      >  7:149519/1‑135 (MQ=255)
                                                                 cTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCagag                                                              >  7:133704/1‑135 (MQ=255)
                                                                                cGTGACGCGGACGAGGCCCGCAAGACGGCCCTGCGGGCGCAGCAGTAAGCGTAAAACCGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAAccgccg                                               <  6:92043/135‑1 (MQ=255)
                                                                                cGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAAccgcc                                                >  3:214019/1‑134 (MQ=255)
                                                                                          aaGAGGCACGCAAGAAGTCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGTCCGCCGCCGCTCCCCGCGACCGCGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGccc                                     >  3:294795/1‑135 (MQ=255)
                                                                                          aaGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGCGCCTGCAGCGCCAGTGGCACCGCCGAAAAAGGccc                                     >  3:11132/1‑135 (MQ=255)
                                                                                          aaGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGCACCGCCGAAAAAGGccc                                     >  1:137507/1‑135 (MQ=255)
                                                                                             agGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTa                                  >  1:63448/1‑135 (MQ=255)
                                                                                                  cGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTcccacc                             >  4:299160/1‑135 (MQ=255)
                                                                                                    cAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCt                           >  8:65390/1‑135 (MQ=255)
                                                                                                    cAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCt                           >  1:207621/1‑135 (MQ=255)
                                                                                                      ccacgccccTGCTGCGGCAGCCGGCCGGGCAAAACCCCCGCCTGCAGGAGGCGCGGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCg                         <  4:79534/129‑1 (MQ=255)
                                                                                                           gCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCg                    <  4:76831/135‑1 (MQ=255)
                                                                                                                  tgGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGCCCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGttt             >  6:287063/1‑135 (MQ=255)
                                                                                                                  tgGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGttt             >  4:26159/1‑135 (MQ=255)
                                                                                                                        agcagGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCg       >  6:287547/1‑135 (MQ=255)
                                                                                                                          cagGAAGCGGAAAACCGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGAt     <  4:116731/135‑1 (MQ=255)
                                                                                                                             gAAGCGGAAAAACGCCGCTAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGACAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGgc  >  6:38430/1‑135 (MQ=255)
                                                                                                                                    |                                                                                                                                
AGCTGCAAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGC  >  ACB122/5755923‑5756183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: