| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 1,529,589 | C→T | 10.2% | L57L (CTG→TTG) | CJ019_01442 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 1,529,589 | 0 | C | T | 10.2% | 112.8 / 4.2 | 39 | L57L (CTG→TTG) | CJ019_01442 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (17/18); new base T (2/2); total (19/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.84e-01 | |||||||||||
TCCTCCTGGTCGCTGCGTGGCGCCCTGGCCCTCGAGCTGGCTGGCGTGCCCTACGAAGAAACTCTGATCAAGCTGAACCAGCCTGATACCCGCCAGCGCATCCTCGCTTTCTCCTCCACCGGCAAGGTGCCGCTGCTCAAGACCGAGCACGGTGTGATCGCCGATTCCCTGGCCATCGCCGAATACCTCAACGAACAGCACCCCGACGCCCAGCTGTGGCCTGCCGACGCAGCCGCCCGCGCCCAGGCCCGTTCGGCTTGCG > ACB122/1529457‑1529718 | tcctccTGGTCGCTGCGTGGCGCCCTGGCCCTCGAGCTGGCTGGCGTGCCCTACGAAGAAACTCTGATCAAGCTGAACCAGCCTGATACACGCCAGCGCATCCTCGCTTTCTCCTCCACCGGCAAGGTGCCgctg > 1:287896/1‑135 (MQ=255) ctGGTCGCTGCGTGGCGCCCTGGCCCTCGAGCTGGCTGGCGTGCCCTACGAAGAAACTCTGATCAAGCTGCACCAGCCTGATACCCGCCAGCGCATCCTCGCTTTCTCCTCCACCGGCAAGGTGCCgctgct > 7:185583/1‑132 (MQ=255) ctGGTCGCTGCGTGGCGCCCTGGCCCTCGAGCTGGCTGGCGTGCCCTACGAAGAAACTCTGATCAAGCTGAACCAGCCTGATACCCGCCAGCGCATCCTCGCTTTCTCCTCCACCGGCAAGGTGCCgctgct > 6:294270/1‑132 (MQ=255) ctGGTCGCTGCGTGGCGCCCTGGCCCTCGAGCTGGCTGGCGTGCCCTACGAAGAAACTCTGATCAAGCTGAACCAGCCTGATACCCGCCAGCGCATCCTCGCTTTCTCCTCCACCGGCAAGGTGCCgctgct > 5:144523/1‑132 (MQ=255) ctGGTCGCTGCGTGGCGCCCTGGCCCTCGAGCTGGCTGGCGTGCCCTACGAAGAAACTCTGATCAAGCTGAACCAGCCTGATACCCGCCAGCGCATCCTCGCTTTCTCCTCCACCGGCAAGGTGCCGCTGCTCaa > 5:118582/1‑135 (MQ=255) ggTCGCTGCGTGGCGCCCTGGCCCTCGAGCTGGCTGGCGTGCCCTACGAAGAAACTCTGATCAAGCTGAACCAGCCTGATACCCGCCAGCGCATCCTCGCTTTCTCCTCCACCGGCAAGGTGCCGCTGCTCAAGa > 1:75998/1‑135 (MQ=255) ggTCGCTGCGTGGCGCCCTGGCCCTCGAGCTGGCTGGCGTGCCCTACGAAGAAACTCTGATCAAGCTGAACCAGCCTGATACCCGCCAGCGCATCCTCGCTTTCTCCTCCACCGGCAAGGTGCCGCTGCTCAAGa > 3:102542/1‑135 (MQ=255) ggTCGCTGCGTGGCGCCCTGGCCCTCGAGATGGGTGGCGTGCCCGCCGAAGAAACTCTGAGCACGCGGAACCCGCCTGCTCCCCGCCAGCGCATCCGCGCGTTCTCCTCCCCCGGCAAGCTGCCGCTGCGCAAGa > 7:113118/1‑135 (MQ=255) tCGCTGCGTGGCGCCCTGGCCCTCGAGCTGGCTGGCGTGCCCTACGAAGAAACTCTGATCAAGCTGAACCAGCCTGATACCCGCCAGCGCATCCTCGCTTTCTCCTCCACCGGCAAGGTGCCGCTGCTCAAGAcc < 3:78635/135‑1 (MQ=255) cGCTGCGTGGCGCCCTGGCCCTCGACCTGGCTGGCGTCCCCTACGAAGAAACTCTGATCAAGCTGAACCAGCCTGATACCCGCCAGCGCATCCTCGCTTTCTCCTCCACCGGCAATGTGCCGCTGCTCAAGAc > 1:49708/1‑133 (MQ=255) tGCGTGGCGCCCTGGCCCTCGAGCTGGCTGGCGTGCCCTACGAAGAAACTCTGATCAAGCTGAACCAGCCCGATACCCGCCAGCGCATCCTCGCTTTCTCCACCACCGGCAAGGTGCCGCTGCTCAAGACCGAGc < 7:258944/135‑1 (MQ=255) cGTGGCGCCCTGGCCCTCGAGCTGGCTGGCGTGCCCTACGAAGAAACTCTGATCAAGCTGAACCAGCCTGATACCCGCCAGCGCATCCTCGCTTTCTCCTCCACCGGCAAGGTGCCGCTGCTCAAGACCGAGCAc > 1:256936/1‑135 (MQ=255) gcCCTGGCCCTCGCGCTGGCTGGCGTGCCCTACGAAGAAACTCTGATCAAGCTGAACCAGCCTGATACCAGCCAGCGCATCCTCGCTTTCTCCTACACCGGCAAGGTGCCGCTGCTCAAGAACGAGCTCGGtgtg > 5:27422/1‑135 (MQ=255) cccTCGAGCTGGCTGGCGTGCCCTACGAAGAAACTCTGATCAAGCTGAACCAGCCTGATACCCGCCAGCGCATCCTCGCTTTCTCCTCCACCGGCAAGGTGCCGCTGCTCAAGACCGAGCACGGTGTGATCGCCg > 6:297861/1‑135 (MQ=255) gctggctgGCGTGCGCTTCGAAGTAACTCTGATCAAGCTGCACCAGCCTGATACCCGCCAGCGCATCGTCGCTTGCTCCTCCACCGGCAAGGTGCCGCTGCTCAAGACCGAGCACGGTGTGATCGCCGATTCCCt < 5:252108/135‑1 (MQ=255) gctggctgGCGTGCCCTACGAAGAAACTCTGATCAAGCTGAACCAGCCTGATACCCGCCAGCGCATCCTCGCTTTCTCCTCCACCGGCAAGGTGCCGCTGCTCAAGACCGAGCCCGGTGTGATCGCCGAGTCCCt > 2:267701/1‑135 (MQ=255) tggctggcGTGCCCTACGAAGAAACTCTGATCAAGCTGAACCAGCCTGATACCCGCCAGCGCATCCTCGCTTTCTCCTCCACCGGCAAGGTGCCGCTGCTCAAGACCGAGCACGGTGTGATCGCCGATTCCCTgg < 4:140168/135‑1 (MQ=255) tggctggcGTGCCCTACGAAGAAACTCTGATCAAGCTGAACCAGCCTGATACCCGCCAGCGCATCCTCGCTTTCTCCTCCACCGGCAAGGTGCCGCTGCTCAAGACCGAGCACGGTGTGATCGCCGATTCCCTgg < 2:256936/135‑1 (MQ=255) ctggcGTGCACTACGAAGAAACTCTGATCAAGCTGAACCAGCCTGATACCCGCCAGCGCATCCTCGCTTTCTCCTCCACCGGCAAGGTGCCGCTGCTCAAGACCGAGCACGGTGTGATCGCCGATTCCCTGGc < 5:217272/133‑1 (MQ=255) tggcGTGCCCTACGAAGAAACTCTGATCAAGCTGAACCAGCCTGATACCCGCCAGCGCATCCTCGCTTTCTCCTCCACCGGCAAGGTGCCGCTGCTCAAGACCGAGCACGGTGTGATCGCCGATTCCCTGGCCAt > 7:185800/1‑135 (MQ=255) ggcGTGCCCTACGAAGAAACTCTGATCAAGCTGAACCAGCCTGATACCCGCCAGCGCATCCTCGCTTTCTCCTCCACCGGCAAGGTGCCGCTGCTCAAGACCGAGGACGGTGTGATCGCCGATTCCCTGGCCATc < 5:279782/135‑1 (MQ=255) cGTGCCCTACGAAGAAACTCTGATCAAGCTGAACCAGCCTGATACCCGCCAGCGCATCCTCGCTTTCTCCTCCACCGGCAAGGTGCCGCTGCTCAAGACCGAGCACGGTGTGATCGCCGATTCCCTGGCCATCg < 5:272249/134‑1 (MQ=255) gTGCCCTACGAAGAAACTCTGATCAAGCTGAACCAGCCTGATACCCGCCAGCGCATCCTCGCTTTCTCCTCCACCGGCAAGGTGCCGCTGCTCAAGACCGAGCACGGTGTGATCGCCGATTCCCTGGCCATCGcc < 3:146490/135‑1 (MQ=255) cTACGAAGAAACTCTGATCAAGCTGAACCAGCCTGATACCCGCCAGCGCATCCTCGCTTTCTCCTCCACCGGCAAGGTGCCGCTGCTCAAGACCGAGCACGGTGTGATCGCCGATTCCCTGGCCATCGCCGAATa < 1:21946/135‑1 (MQ=255) tCAAGCTGAACCAGCCTGATACCCGCCAGCGCATCCTCGCTTTCTCCTCCACGGGCAAGGTGCCGCTGCTCAAGACCGAGCACGGTGTGATCGCCGATTCCCTGGCCATCGCCGAATACCTCAACGAACAGCa < 2:49708/133‑1 (MQ=255) gATACCCGCCAGCGCATCCTCGCTTTCTCCTCCACCGGCAAGGTGCCGCTGCTCAAGACCGAGCACGGTGTGATCGCCGATTCCCTGGCCATCGCCGAATACCTCAACGAACAGCACCCCGACGCCCAGCTGTgg > 7:160108/1‑135 (MQ=255) gATACCCGCCAGCGCATCCTCGCTTTCTCCTCCACCGGCAAGGTGCCGCTGCTCAAGACCGAGCACGGTGTGATCGCCGATTCCCTGGCCATCGCCGAATACCTCAACGAACAGCACCCCGACGCCCAGCTGTgg > 6:177854/1‑135 (MQ=255) cccGCCAGCGCATCCTCGCTTTCTCCTCCACCGGCAAGGTGCCGCTGCTCAAGACCGAGCACGGTGTGATCGCCGATTCCCTGGCCATCGCCGAATACCTc < 7:274380/101‑1 (MQ=255) cccGCCAGCGCATCCTCGCTTTCTCCTCCACCGGCAAGGTGCCGCTGCTCAAGACCGAGCACGGTGTGATCGCCGATTCCCTGGCCATCGCCGAATACCTc > 8:274380/1‑101 (MQ=255) gcgcATCCTCGCTTTCTCCTCCACCGGCAAGGTGCCGCTGCTCAAGACCGAGCACGGTGTGATCGCCGATTCCCTGGCCATCGCCGAATACCTCAACGAACAGCACCCCGACGCCCAGCTGTGGCCTGCCGACGc < 5:177854/135‑1 (MQ=255) aTCCTCGCTTTCTCCTCCACCGGCAAGGTGCCGCTGCTCAAGACCGAGCACGGTGTGATCGCCGATTCCCTGGCCATCGCCGAATACCTCAACGAACAGCACCCCGACGCCCAGCTGTGGCCTGCCGAccccccc > 2:269122/1‑129 (MQ=255) aTCCTCGCTTTCTCCTCCACCGGCAAGGTGCCGCTGCTCAAGACCGAGCACGGTGTGATCGCCGATTCCCTGGCCATCGCCGAATACCTCAACGAACAGCACCCCGACGCCCAGCTGTGGCCTGCCGACGCAgcc > 5:107085/1‑135 (MQ=255) tCCTCGCTTTCTCCTCCACCGGCAAGGTGCCGCTGCTCAAGACCGAGCACGGTGTGATCGCCGATTCCCTGGCCATCGCCGAATACCTCAACGAACAGCACCCCGACGCCCAGCTGTGGCCTGCCGACGCAgccg < 5:220630/135‑1 (MQ=255) gCTTTCTCCTCCACCGGCAAGGTGCCGCTGCTCAAGACCGAGCACGGTGTGATCGCCGATTCCCTGGCCATCGCCGAATACCTCAACGAACAGCACCCCGACGCCCAGCTGTGGCCTGCCGACGCAGccgcccgc < 5:294270/135‑1 (MQ=255) tctcCTCCACCGGCAAGGTGCCGTTGCTCAAGACCGa < 6:164241/37‑1 (MQ=255) tctcCTCCACCGGCAAGGTGCCGTTGCTCAAGACCGa < 6:160669/37‑1 (MQ=255) tctcCTCCACCGGCAAGGTGCCGTTGCTCAAGACCGa > 5:164241/1‑37 (MQ=255) tctcCTCCACCGGCAAGGTGCCGTTGCTCAAGACCGa > 5:160669/1‑37 (MQ=255) cACCGGCAAGGTGCCGCTGCTCAAGACCGAGCACGGTGTGATCGCCGATTCCCTGGCCATCGCCGAATACCTCAACGAACAGCACCCCGACGCCCAGCTGTGGCCTGCCGACGCAGCCGCCCGCGCCCAGGCCCg < 8:115256/135‑1 (MQ=255) ccGGCAAGGTGCCGCTGCTCAAGACCGAGCACGGTGTGATCGCCGATTCCCTGGCCATCGCCGAATACCTCAACGAACAGCACCCCGACGCCCAGCTGTGGCCTGCCGACGCAGCCGCCCGCGCCCAGGCCCGtt > 6:216010/1‑135 (MQ=255) aGGTGCCGCTGCTCAAGACCGAGCACGGTGTGATCGCCGATTCCCTGGCCATCGCCGAATACCTCAACGAACAGCACCCCGACGCCCAGCTGTGGCCTGCCGACGCAGCCGCCCGCGCCCAGGCCCGTTCGGCtt < 2:75998/135‑1 (MQ=255) aGGTGCCGCTGCTCAAGACAGAGCACGGTGTGATCGCCGATTCCCTGGCCATCGCCGAATACCTCAACGAACAGCACCCCGACGCCCAGCTGTGGCCTGCCGACGCAGCCGCCCGCGCCCAGGCCCGTTCGGCtt > 7:99771/1‑135 (MQ=255) tGCCGCTGCTCAAGACCGAGCACGGTGTGATCGCCGATTCCCTGGCCATCGCCGAATACCTCAACGAACAGCACCCCGACGCCCAGCTGTGGCCTGCCGACGCAGCCGCCCGCGCCCAGGCCCGTTCGGCTTgcg < 6:144523/135‑1 (MQ=255) | TCCTCCTGGTCGCTGCGTGGCGCCCTGGCCCTCGAGCTGGCTGGCGTGCCCTACGAAGAAACTCTGATCAAGCTGAACCAGCCTGATACCCGCCAGCGCATCCTCGCTTTCTCCTCCACCGGCAAGGTGCCGCTGCTCAAGACCGAGCACGGTGTGATCGCCGATTCCCTGGCCATCGCCGAATACCTCAACGAACAGCACCCCGACGCCCAGCTGTGGCCTGCCGACGCAGCCGCCCGCGCCCAGGCCCGTTCGGCTTGCG > ACB122/1529457‑1529718 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |