Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	1,619,115	T→G	13.6%	E260D (GAA→GAC)	nadB ←	L‑aspartate oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	1,619,115	0	T	G	13.6%	119.3 / 3.4	61	E260D (GAA→GAC)	nadB	L‑aspartate oxidase
Reads supporting (aligned to +/- strand):  ref base T (17/34);  new base G (8/2);  total (25/36)
Fisher's exact test for biased strand distribution p-value = 1.12e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGGCGCTTCATCTCGTGGTCGATGGCGCGGGCCACGATGTCCCGTGGGGCCAGCTCTTCGCGTGGGTCGAAGCGTGGCATGAAACGTTCGCCGTTGGGCAGGCGCAGCAGGGCGCCCTCGCCGCGCAGGGCTTCGGTGATCAGGAAGCTCTTGGCCTGTGGGTGATACAGGCAGGTCGGGTGGAACTGGTTGAATTCCAGGTTCGCCACTCGGCAGCCGGCCCGCCAGGCCATGGCGATACCGTCGCCGCAGGCACCATCGGGGTT  >  ACB122/1618983‑1619249                                                                                                                                     |                                                                                                                                       cAGGCGCTTCATCTCGTGGTCGATGGCGCGGGCCACGATGTCCCGTGGGGCCAGCTCTTCGCGTGGGTCGAAGCGTGGCATGAAACGTTCGCCGTTGGGCAGGCGCCGCCGGGCGCCCTCGCCGCGCAGGGCGTc                                                                                                                                      >  6:64996/1‑135 (MQ=255) cAGGCGCTTCATCTCGTGGTCGATGGCGCGGGCCACGATGTCCCGTGGGGCCAGCTCTTCGCGTGGGTCGAAGCGTGGCATGAAACGTTCGCCGTTGGGCAGGCGCAGCAGGGCGCCCTCGCCGCGCAGGGCTTc                                                                                                                                      <  7:5744/135‑1 (MQ=255) cAGGCGCTTCATCTCGTGGTCGATGGCGCGGGCCACGATGTCCCGTGGGGCCAGCTCTTCGCGTGGGGCGAAGCGGGGCATGAAACGTTCGCCGTTGGGCAGGCGCAGCAGGGCGCCCTCGCCGCGCAGGGCGTc                                                                                                                                      >  1:204546/1‑135 (MQ=255)      gcTTCATCTCGTGGTCGATGGCGCGGGCCACGATGTCCCGTGGGGCCAGCTCTTCGCGTGGGTCGAAGCGTGGCATGAAACGTTCGCCGTTGGGCAGGCGCCGCAGGGCGCCCGCGCCGCGCCGGGCGTCGGTGa                                                                                                                                 >  8:5744/1‑135 (MQ=255)      gcTTCATCTCGTGGTCGATGGCGCGGGCCACGATGTCCCGTGGGGCCAGCTCTTCGCGTGGGTCGAAGCGTGGCATGAAACGTTCGCCGTTGGGCAGGCGCAGCCGGGCGCCCTCGCCGCGCAGGGCTGCGGGGa                                                                                                                                 >  3:240836/1‑135 (MQ=255)      gcTTCATCTCGTGGTCGATGGCGCGGGCCACGATGTCCCGTGGGGCCAGCTCTTCGCGTGGGTCGAAGCGTGGCATGAAACGTTCGCCGTTGGGCAGGCGCAGCCGGGCGCCCGCGCCGCGCCGGGGGTCggggg                                                                                                                                 >  5:168787/1‑132 (MQ=255)      gcTTCATCTCGTGGTCGATGGCGCGGGCCACGATGTCCCGTGGGGCCAGCTCTTCGCGTGGGTCGAAGCGTGGCATGAAACGTTCGCCGTTGGGCAGGCGCAGCAGGGCGCCCTCGCCGCGCCGGGCGTCGGTgc                                                                                                                                 >  1:98156/1‑134 (MQ=255)      gcTTCATCTCGTGGTCGATGGCGCGGGCCACGATGTCCCGTGGGGCCAGCTCTTCGCGTGGGTCGAAGCGTGGCATGAAACGTTCGCCGTTGGGCAGGCGCAGCAGGGCGCCCTCGCCGCGCAGGGCTTCGGTgc                                                                                                                                 >  3:98411/1‑134 (MQ=255)      gcTTCATCTCGTGGTCGATGGCGCGGGCCACGATGTCCCGTGGGGCCAGCTCTTCGCGTGGGTCGAAGCGTGGCATGAAACGTTCGCCGTTGGGCAGGCGCAGCAGGGCGCCCTCGCCGCGCAGGGCTTCGGTGa                                                                                                                                 >  7:104885/1‑135 (MQ=255)      gcTTCATCTCGTGGTCGATGGCGCGGGCCACGATGTCCCGTGGGGCCAGCTCTTCGCGTGGGTCGAAGCGTGGCATGAAACGTTCGCCGTTGGGCAGGCGCAGCAGGGCGCCCTCGCCGCGCAGGGCTTCGGTGa                                                                                                                                 >  2:218798/1‑135 (MQ=255)      gcTTCATCTCGTGGTCGATGGCGCGGGCCACGATGTCCCGTGGGGCCAGCTCTTCGCGTGGGTCGAAGCGTGGCATGAAACGTTCGCCGTTGGGCAGGCGCAGCAGGGCGCCCTCGCCGCGCAGGGCTTCGGTGa                                                                                                                                 >  7:170733/1‑135 (MQ=255)      gcTTCATCTCGTGGTCGATGGCGCGGGCCACGATGTCCCGTGGGGCCAGCTCTTCGCGTGGGTCGAAGCGTGGCATGAAACGTTCGCCGTTGGGCAGGCGCAGCAGGGCGCCCTCGCCGCGCAGGGCTTCGGTGa                                                                                                                                 >  2:51428/1‑135 (MQ=255)      gcTTCATCTCGTGGTCGATGGCGCGGGCCACGATGTCCCGTGGGGCCAGCTCTTCGCGTGGGTCGAAGCGTGGCATGAAACGTTCGCCGTTGGGCAGGCGCAGCAGGGCGCCCTCGCCGCGCAGGGCTGCGCTGa                                                                                                                                 >  3:103346/1‑135 (MQ=255)      gcTTCATCTCGTGGTCGATGGCGCGGGCCACGATGTCCCGTGGGGCCAGCTCTTCGCGTGGGTCGAAGCGTGGCATGAAACGTTCGCCGTTGGGCAGGCGCAGCAGGGCGCCCTCGCCGCGCAGGGCGTCGGTGa                                                                                                                                 >  8:73755/1‑135 (MQ=255)      gcTTCATCTCGTGGTCGATGGCGCGGGCCACGATGTCCCGTGGGGCCAGCTCTTCGCGTGGGTCGAAGCGGGGCCTGAAACGTTCGCCGTTGGGCAGGCGCAGCAGGGCGCCCTCGCCGCGCCGGGCTGCggggg                                                                                                                                 >  5:201017/1‑132 (MQ=255)      gcTTCATCTCGTGGTCGATGGCGCGGGCCACGAAGTCCCGTGGGGCCAGCTCTTCGCGTGGGTCGAAGCGTGGCATGAAACCTTCGCCGTTGGGCAGGCGCAGCAGGTCGACCTCGCCGCGCAGGGCGTCGGTGa                                                                                                                                 >  3:276869/1‑135 (MQ=255)           aTCTCGTGGTCGATGGCGCGGGCCACGATGTCCCGTGGGGCCAGCTCTTCGCGTGGGTCGAAGCGTGTCATGAAACGTTCGCCGTTGGGCAGGCGCAGCAGGGCGCCCTCGCCGCGCAGGGCTTCGGTGATCa                                                                                                                              <  1:250403/133‑1 (MQ=255)           aTCTCGTGGTCGATGGCGCGGGCCACGATGTCCCGTGGGGCCAGCTCTTCGCGTGGGTCGAAGCGTGGCATGAAACGTTCGCCGTTGGGCAGGCGCAGCAGGGCGCCCTCGCCGCGCAGGGCTTCGGTGATCa                                                                                                                              <  6:241980/133‑1 (MQ=255)           aTCTCGTGGTCGATGGCGCGGGCCACGATGTCCCGTGGGGCCAGCTCTTCGCGTGGGTCGAAGCGTGGCATGAAACGTTCGCCGTTGGGCAGGCGCAGCAGGGCGCCCTCGCCGCGCAGGGCTTCGGTGATCa                                                                                                                              <  3:265520/133‑1 (MQ=255)            tctcGTGGTCGATGGCGCGGGCCACGATGTCCCGTGGGGCCAGCTCTTCGCGTGGGTCGAAGCGTGGCATGAAACGTTCGCCGTTGGGCAGGCGCAGCAGGGCGCCCTCGCCGCGCAGGGCTTCGGTGATCAgg                                                                                                                            <  8:110986/134‑1 (MQ=255)                  ggTCGATGGCGCGGGCCACGATGTCCCGTGGGGCCAGCTCTTCGCGTGGGTCGAAGCGTGGCATGAAACGTTCGCCGTTGGGCAGGCGCAGCAGGGCGCCCTCGCCGCGCACGGCTTCGGTGATCAGGAAGctct                                                                                                                     >  5:59049/1‑135 (MQ=255)                   gTCGATGGCGCGGGCCACGATGTCCCGTGGGGCCAGCTCTTCGCGTGGGTCGAAGCGTGGCATGAAACGTTCGCCGTTGGGCAGGCGCAGCAGGGCGCCCTCGCCGCGCAGGGCTTCGGTGATCAGGAAGCTCtt                                                                                                                    <  7:122385/135‑1 (MQ=255)                     cGATGGCGCGGGCCACGATGTCCCGTGGGGCCAGCTCTTCGCGTGGGTCGAAGCGTGGCATGAAACGTTCGCCGTTGGGCAGGCGCAGCAGGGCGCCCTCGCCGCGCAGGGCTTCGGTGATCAGGAAGCTCTTgg                                                                                                                  <  6:225714/135‑1 (MQ=255)                     cGATGGCGCGGGCCACGATGTCCCGTGGGGCCAGCTCTTCGCGTGGGTCGAAGCGTGGCATGAAACGTTCGCCGTTGGGCAGGCGCAGCAGGGCGCCCTCGCCGCGCAGGGCTTCGGTGATCAGGAAGCTCTTgg                                                                                                                  <  6:59049/135‑1 (MQ=255)                         ggCGCGGGCCACGATGTCCCGTGGGGCCAGCTCTTCGCGTGGGTCGAAGCGTGGCATGAAACGTTCGCCGTTGGGCAGGCGCAGCCGGGCGCCCTCGCCGCGCCGGGCGGCGGGGGTCCGGAAGCTCTTGGccgg                                                                                                              >  5:21943/1‑133 (MQ=255)                         ggCGCGGGCCACGATGTCCCGTGGGGCCAGCTCTTCGCGTGGGTCGAAGCGTGGCATGAAACGTTCGCCGTTGGGCAGGCGCAGCAGGGCGCCCTCGCCGCGCAGGGCTTCGGTGATCAGGAAGCTCTTGGCCtg                                                                                                              <  3:204739/135‑1 (MQ=255)                          gcgcGGGCCACGATGTCCCGTGGGGCCAGCTCTTCGCGTGGGTCGAAGCGTGGCATGAAACGTTCGCCGTTGGGCAGGCGCAGCAGGGCGCCCTCGCCGCGCAGGGCTTCGGTGATCAGGAAGCTCTTGGCCtg                                                                                                              <  3:175635/134‑1 (MQ=255)                           cgcgGGCCACGATGTCCCGTGGGGCCAGCTCTTCGCGTGGGTCGAAGCGTGGCATGAAACGTTCGCCGTTGGGCAGGCGCAGCAGGGCGCCCTCGCCGCGCAGGGCTGCGGTGATGAGGAAGCTCTTGGCCggtg                                                                                                            >  6:245044/1‑135 (MQ=255)                           cgcgGGCCACGATGGCCCGTGGGGCCAGCTCTTCGCGTGGGTCGAAGCGTGGCATGAAACGTTCGCCGTTGGGCAGGCGCAGCAGGGCGCCCTCGCCGCGCAGGGCTTCGGTGATCAGGAAGCTCTTGGCCtgt                                                                                                             <  5:34464/134‑1 (MQ=255)                           cgcgGGCCACGATGGCCCGTGGGGCCAGCTCTTCGCGTGGGTCGAAGCGTGGCATGAAACGTTCGCCGTTGGGCAGGCGCAGCAGGGCGCCCTCGCCGCGCAGGGCTTCGGTGATCAGGAAGCTCTTGGCCtg                                                                                                              <  1:301502/133‑1 (MQ=255)                              gggCCTCGATGTCCCGTGGGGCCAGCGCTTCGCGTGGGTCGAAGCGTGGCATGAAACGTTCGCCGTTGGGCAGGCGCAGCAGGGCGCCCTCGCCGCGCAGGGCTTCGGTGATCAGGAAGCTCTTGGCCTGTGGGt                                                                                                         <  1:251815/135‑1 (MQ=255)                                           ccGTGGGGCCAGCTCTTCGCGTGGGTCGAAGCGTGGCATGAAACGTTCGCCGTTGGGCAGGCGCAGCAGGGCGCCCTCGCCGCGCAGGGCTTCGGTGATCAGGAAGCTCTTGGCCTGTGGGTGATACAGGCAGGt                                                                                            <  2:201953/135‑1 (MQ=255)                                                 ggCCAGCTCTTCGCGTGGGTCGAAGCGTGGCATGAAACGTTCGCCGTTGGGCAGGCGCAGCAGGGCGCCCTCGCCGCGCAGGGCTTCGGTGATCAGGAAGCTCTTGGCCTGTGGGTGATACAGGCAGGTCGGGTg                                                                                      <  8:281113/135‑1 (MQ=255)                                                      gCTCTTCGCGTGGGTCGAAGCGTGGCATGAAACGTTCGCCGTTGGGCAGGCGCAGCAGGGCGCCCTCGCCGCGCAGGGCTTCGGTGATCAGGAAGCTCTTGGCCTGTGGGTGATACAGGCAGGTCGGGTGGCACt                                                                                 >  8:249716/1‑135 (MQ=255)                                                       ctctTCGCGTGGGTCGAAGCGTGGCATGAAACGTTCGCCGTTGGGCAGGCGCAGCAGGGCGCCCTCGCCGCGCAGGGCTTCGGTGATCAGGAAGCTCTTGGCCTGTGGGTGATACAGGCAGGTCGGGTGGAACTg                                                                                <  4:297612/135‑1 (MQ=255)                                                        tctTCGCGTGGGTCGAAGCGTGGCATGAAACGTTCGCCGTTGGGCAGGCGCAGCAGGGCGCCCTCGCCGCGCAGGGCTTCGGTGATCAGGAAGCTCTTGGCCtg                                                                                                              >  8:66327/1‑104 (MQ=255)                                                        tctTCGCGTGGGTCGAAGCGTGGCATGAAACGTTCGCCGTTGGGCAGGCGCAGCAGGGCGCCCTCGCCGCGCAGGGCTTCGGTGATCAGGAAGCTCTTGGCCtg                                                                                                              <  7:66327/104‑1 (MQ=255)                                                               gTGGGTCGAAGCGTGGCATGAAACGTTCGCCGTTGGGCAGGCGCAGCAGGGCGCCCTCGCCGCGCAGGGCTTCGGTGATCAGGAAGCTCTTGGCCTGTGGGTGATACAGGCAGGTCGGGTGGAACTGGTTGAAtt                                                                        >  8:103179/1‑135 (MQ=255)                                                                         gCGTGGCATGAAACGTGCGCCGTTGGGCAGGCGCAGCAGGGCGCCCTCGCCGCGCAGGGCTTCGGTGATCAGGAAGCTCTTGGCCTGTGGGTGATACAGGCAGGTCGGGTGCAACTGGTTGAATTCCAGGTTCGc                                                              <  8:83399/135‑1 (MQ=255)                                                                                         tCGCCGTTGGGCAGGCGCAGCAGGGCGCCCTCGCCGCGCAGGGCTTCGGTGATCAGGAAGCTCTTGGCCTGTGGGTGATACAGGCAGGTCGGGTGGAACTGGTTGAATTCCAGGTTCGCCACTCGGCAGCCGGcc                                              <  4:68917/135‑1 (MQ=255)                                                                                         tCGCCGTGGGGCAGGCGCAGCAGGGGGCCCTCGCCGCGCAGGGCTTCGGTGATCAGGAAGCGCTTGGCCTGGGGGTTATACAGGCAGTTCGGGCGGAACTGGTTGAATACCAGGTTCGCCACTCGGCAGCCGGcc                                              <  8:141229/135‑1 (MQ=255)                                                                                              ggtGGGCAGGCGCAGCAGGGCGCCCGCGCCGCGCCGGGCTGCGGGGATCAGGACGCTCTTGGCCTGTGGGGGATCCAGGCAGGTCGGGTGGAACTGGTTGAATTCCAGGTTCGCCACTCGGCAGCCGGCCCGCCa                                         <  5:64996/133‑1 (MQ=255)                                                                                              gTTGGGCCGGCGCAGCAGGGCGCCCTCGCCGCGCAGGGCTTCGGTGATCAGGAAGCGCTGGGCCTGGGGGTGAAACAGGCAGGGCGGGTGGAACTGGTTGAATTCCAGGTGCGCCACTCGGCAGCCGGCCCGCCa                                         <  6:168787/135‑1 (MQ=255)                                                                                              gTTGGGCAGGCGCAGCAGGGCGCCCTCGCCGCGCAGGGCTTCGGTGATCAGGAAGCTCTTGGCCTGTGGGTGATCCAGGCAGGTCGGGTGGAACTGGTTGAATTCCAGGTTCGCCACTCGGCAGCCGGCCCGCCa                                         <  8:170733/135‑1 (MQ=255)                                                                                              gTTGGGCAGGCGCAGCAGGGCGCCCTCGCCGCGCAGGGCGTCGGTGATCAGGACGCTCTTGGCCTGTGGGGGATACAGGCAGGGCGGGTGGAACTGGTTGAATTCCAGGTTCGCCACTCGGCAGCCGGCCCGCCa                                         <  2:204546/135‑1 (MQ=255)                                                                                                tGGGCAGGCGCAGCAGGGCGCCCTCGCCGCGCAGGGCTTCGGTGATCAGGAAGCTCTTGGCCTGTGGGTGATACAGGCAGGTCGGGTGGAACTGGTTGAATTCCAGGTTCGCCACTCGGCAGCCGGCCCGCCAgg                                       <  2:63119/135‑1 (MQ=255)                                                                                                                    cccTCGCCGCGCAGGGCTTCGGTGATCAGGAAGCTCTTGGCCTGTGGGTGATGCAGGCAGGTCGGGTGGAACTGGTTGAATTCCAGGTTCGCCACTCGGCAGCCGGCCCGCCAGGCCATGGCGATACCGTcgccg                   >  7:34742/1‑135 (MQ=255)                                                                                                                     ccTCGCCGCGCAGGGCTTCGGTGATCAGGAAGCTCTTGGCCTGTGGGTGATACAGGCAGGTCGGGTGGAACTGGTTGAATTCCAGGTTCGCCACTCGGCAGCCGGCCCGCCAGGCCATGGCGATACCGTcgccgc                  <  3:260441/135‑1 (MQ=255)                                                                                                                        cgccgcGCAGGGCTTCGGTGATCAGGAAGCTCTTGGGCTGTGGGTGATACAGGCAGGTCGGGTGGAACTGGTTGAATTCCAGGTTCGCCACTCGGCAGCCGGCCCGCCCGGCCATGGCGATCCCGTCGCCGCAgg               >  5:76453/1‑135 (MQ=255)                                                                                                                            gcgcAGGGCTTCGGTGATCAGGAAGCTCTTGGCCTGTGGGTGATACAGGCAGGTCGGGTGGAACTGGTTGAATTCCAGGTTCGCCACTCGGCAGCCGGCCCGCCAGGCCATGGCGATACCGTCGCCGCAGGCAcc           >  4:94642/1‑135 (MQ=255)                                                                                                                            gcgcAGGGCGTCGGTGATCAGGAAGCTCTTGGCCTGTGGGTGATACAGGCAGGGCGGGTGGAACTGGTTGAATTCCAGGTTCGCCACTCGGCAGCCGGCCCGCCAGGCCATGGCGATACCGTCGCCGCAGGCAcc           <  2:234743/135‑1 (MQ=255)                                                                                                                             cgcAGGGCTTCGGTGATCAGGAAGCTCTTGGCCTGTGGGTGATACAGGCAGGTCGGGTGGAACTGGTTGAATTCCAGGTTCGCCACTCGGCAGCCGGcc                                              <  7:80161/99‑1 (MQ=255)                                                                                                                             cgcAGGGCTTCGGTGATCAGGAAGCTCTTGGCCTGTGGGTGATACAGGCAGGTCGGGTGGAACTGGTTGAATTCCAGGTTCGCCACTCGGCAGCCGGcc                                              >  8:80161/1‑99 (MQ=255)                                                                                                                                 tggCTTCGGTGATCAGGAAGCTCTTGGCCTGTGGGTGATACAGGCTGGTCGGGTGGAACTGGTTGAATTACAGGTTAGCCACTCGGCAGCCGTCACTCCAGGCCATGGCGATACCGTCGCCGCAGGCACCATCgg      <  6:207333/134‑1 (MQ=255)                                                                                                                                 gggCTTCGGTGATCAGGAAGCTCTTGGCCTGTGGGTGATACAGGCAGGTCGGGTGGAACTGGTTGAATTCCAGGTTCGCCACTCGGCAGCCGGCCCGCCAGGCCATGGCGATACCGTCGCCGCAGGCACCATCgg      <  1:218798/135‑1 (MQ=255)                                                                                                                                   gCTTCGGTGATCAGGAAGCTCTTGGCGTGTGGGTGATACAGGCAGTGCGGGTGGAACTGGTTGAATTCCAGGTTCGCCACTCGGCAGCCGGCCCGCCAGGCCATGGCGATACCGTCGCCGCAGGCACCATCgggg    <  2:219998/135‑1 (MQ=255)                                                                                                                                   gCTTCGGTGATCAGGAAGCTCTTGGCCTGTGGGTGATACAGGCAGGTCGGGTGGAACTGGTTGAATTCCAGGTTCGCCACTCGGCAGCCGGCCCGCCAGGCCATGGCGATACCGTCGCCGCAGGCACCATCgggg    <  1:220531/135‑1 (MQ=255)                                                                                                                                   gCTTCGGTGATCAGGAAGCTCTTGGCCTGTGGGTGATACAGGCAGGTCGGGTGGAACTGGTTGAATTCCAGGTGCGCCACTCGGCAGCCGGCCCGCCAGGCCATGGCGATACCGTCGCCGCAGGCACCATCgggg    <  2:15741/135‑1 (MQ=255)                                                                                                                                    cTTCGGTGATCAGGAAGCTCTTGGCCTGTGGGTGATGCAGGCAGGTCGGGGGGAACTGGTTGAATTCCAGGTTCGCCACTCGGCAGCCGGCCCGCCAGGCCATGGCGATACCGTCGCCGCAGGCACCATCGGGGt   <  8:34742/135‑1 (MQ=255)                                                                                                                                    cTTCGGTGATCAGGAAGCTCTTGGCCTGTGGGTGATACAGGCAGGTCGGGTGGAACTGGTTGAATTCCAGGTTCGCCACTCGGCAGCCGGCCCGCCAGGCCATGGCGATACCGTCGCCGCAGGCACCATCGGGGt   <  3:94642/135‑1 (MQ=255)                                                                                                                                    cTTCGGTGATCAGGAAGCTCTTGGCCTGTGGGTGATACAGGCAGGTCGGGTGGAACTGGTTGAATTCCAGGTTCGCCACTCGGCAGCCGGCCCGCCAGGCCATGGCGATACCGTCGCCGCAGGCACCATCGGGGt   <  6:155795/135‑1 (MQ=255)                                                                                                                                     ttCGGTGATCAGGAAGCTCTTGGCCTGTGGGTGATACAGGCAGGTCGGGTGGAACTGGTTGAATTCCAGGTTCGCCACTCGGCAGCCGGCCCGCCAGGCCATGGCGATACCGTCGCCGCAGGCACCATCGGGGtt  <  8:196876/135‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       CAGGCGCTTCATCTCGTGGTCGATGGCGCGGGCCACGATGTCCCGTGGGGCCAGCTCTTCGCGTGGGTCGAAGCGTGGCATGAAACGTTCGCCGTTGGGCAGGCGCAGCAGGGCGCCCTCGCCGCGCAGGGCTTCGGTGATCAGGAAGCTCTTGGCCTGTGGGTGATACAGGCAGGTCGGGTGGAACTGGTTGAATTCCAGGTTCGCCACTCGGCAGCCGGCCCGCCAGGCCATGGCGATACCGTCGCCGCAGGCACCATCGGGGTT  >  ACB122/1618983‑1619249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑