Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,630,540 A→C 36.8% L419R (CTG→CGG)  czcS ← Sensor protein CzcS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,630,5400AC36.8% 28.6 / 4.1 38L419R (CTG→CGG) czcSSensor protein CzcS
Reads supporting (aligned to +/- strand):  ref base A (13/10);  new base C (10/5);  total (23/15)
Fisher's exact test for biased strand distribution p-value = 7.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTCCAGCCGCGAACGCCCCGAACCTA‑CCCGGTAAAACCGCTCGAACAAGCGCGACTGGTGCTCTGCAGGCACACCCGGACCCTGGTTCTCCACGGATAGAATGACTTCGCTGCCCGAGCGTTCGATGCGCAGAG  >  ACB122/1630408‑1630669
                                                                                                                                    |                                                                                                                                  
gcCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCCACCCCGTACCCCCCACCCGc                                                                                                                                  >  8:141434/1‑135 (MQ=255)
gcCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCCGTCCCGCCCTCCAGc                                                                                                                                  >  1:223859/1‑135 (MQ=255)
  cGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTCCAGCcg                                                                                                                                <  1:183652/135‑1 (MQ=255)
   gATGACTCGGCAGGAAACACCAGTGTAAACAGGGGATGCCCCAGGGGGCAGCTGATGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCACAACCCGGTCCCGCCCTCCAGCcgc                                                                                                                               <  8:268406/135‑1 (MQ=255)
   gATCACTCGGCAGGAAACACCAGGGTAAAACGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTCCAGCcgc                                                                                                                               <  4:81508/135‑1 (MQ=255)
                gAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTCCAGCCGCGAACGCCCCGAAc                                                                                                                  <  7:141434/135‑1 (MQ=255)
                gAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTCCAGCCGCGAACGCCCCGAAc                                                                                                                  <  3:283911/135‑1 (MQ=255)
                gAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTCCAGCCGCGAACGCCCCGAAc                                                                                                                  <  3:180566/135‑1 (MQ=255)
                gAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCGCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTCCAGCCGCGAACGCCCCGAAc                                                                                                                  <  3:88751/135‑1 (MQ=255)
                      ccAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCACCCCCCACCCCGTCCCCCCCGCACGCCCCGCACCCCCCGCaccca‑ccc                                                                                                           >  5:34676/1‑135 (MQ=255)
                          ggTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTCCAGCCGCGAACGCCCCGAACCTA‑CCCGGTa                                                                                                       <  4:244563/135‑1 (MQ=255)
                          ggTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCCGGACCGCCCACCCGCCGCGAACGCCCCCACCCAA‑CCCCGt                                                                                                        >  2:132592/1‑134 (MQ=255)
                          ggTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCACACCCCGGTCCCGCCCACCCGCCGCGCACCGCCCGCACCGA‑ccccgg                                                                                                        >  7:274487/1‑131 (MQ=255)
                             aaaCCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTCCCGCCCCGCACGCCCCGGACCAACCCCGGTaaa                                                                                                     >  1:110939/1‑135 (MQ=255)
                               aCCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCCACCCCGTACCGCCCTCCAGCCGCGAACCCCCCGAACCTA‑CCCGGGAAAAcc                                                                                                  >  6:80223/1‑135 (MQ=255)
                                  gggTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCCACCCCGTCCCGCCCGCCCGCCGCGCACCCCCCGCACCGA‑CCCGGTAAAACCGcg                                                                                               >  7:74871/1‑134 (MQ=255)
                                  gggTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTCCAGCCGCGAACGCCCCGAACCTA‑CCCGGTAAAACCGCt                                                                                               >  4:146698/1‑135 (MQ=255)
                                    gTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCTCCAACCCCAACCCGGTCCCGCCCTCCAGCCGCGAACGCCCCGCACCTA‑CCCGCTTAAACCGCTCg                                                                                             >  2:175534/1‑135 (MQ=255)
                                    gTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTCCAGCCGCGCACCCCCCGAACCGA‑CCCGGTAAAACCGCTc                                                                                              >  8:106413/1‑134 (MQ=255)
                                       ggCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCCACCCGGTGCCGCCCGCCCGCCGCGGCCGCCCCGCACCGA‑CCCCGGAAAACcgcgcgca                                                                                           >  6:175235/1‑132 (MQ=255)
                                       ggCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTCCCGCCGCGACCGCCCCGACCCGA‑CCCGGTAAAACCCCTccca                                                                                           >  2:191919/1‑131 (MQ=255)
                                       ggCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCCGTCCCGCCCACCAGCCGCGGACGCCCCGCACCCA‑ACCCGGAAAACCGCTCgca                                                                                           >  4:54926/1‑132 (MQ=255)
                                             aGGGGGCAGCTGCTGACCTCGCCCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCCACCCCCACCCGGTCCCGCCCGCCCGCCGCGCACCCCCCCCCCCGA‑CCCCGGAAAACCGCGCGCCCACgcgc                                                                                    >  1:110235/1‑135 (MQ=255)
                                                gggCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGCTCGCCAACCCCCCCCCGGTCCCGCCCGCCAGCCGCGAGCGCCCCGCCCCGC‑CCCGGGAAAACCGCTCGCACCAGcgcgcc                                                                                 >  2:75123/1‑133 (MQ=255)
                                                gggCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCACCCCCACCCCGGTCCCGCCCTCCCGCCGCGCACGCCCCGAACCCA‑CCCGGTAAAACCGCGCCCACAAGcgcg                                                                                   >  7:245337/1‑133 (MQ=255)
                                                   cAGCGGCGGACCGCGACCCGCCCCCCATGCAACTGCATGAGCGACTTCACGAGCGCCAACCCCAACCCGGTCCCGCCCTCCCGCCGCCAACGCCCCGAACCTA‑CCCGGTAAAACCGCGCGAACAAGCGCGACTgg                                                                              <  8:294655/135‑1 (MQ=255)
                                                           gACCCCGACCCGCCCGCCATGCACCTGCATGAGCGACTACACCACCGCCAACCCCACCCCGGCCCCGCCCGCCCGCCCCGACCCCCCCGACCCTC‑CCCGTTAAACCCGCTCGAACACCCGCGACTGGTGCTCTGc                                                                      <  3:54926/135‑1 (MQ=255)
                                                             ccTCGCCCCCCCCGCCATGCAACTGCATGATCGACTTCACGAGCGCCAACCCCACCCCGGCCCCGCCCTCCCCCCGCGACCGCCCCCAACCTC‑CCCGGTAAAACCGCTCCAACAAGCGCGACTGGTGCTCTGCAg                                                                    <  6:67485/135‑1 (MQ=255)
                                                                    ccgcccgcCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAAACCGGTCCCGCCCGCCAGCCCCGCACGCCCCCAACCTA‑CCCGGTAAAACCGCTCGAACCAGCGCGCCAGGTGCTCTGCAGGCCCAcc                                                             >  8:222308/1‑135 (MQ=255)
                                                                          gccATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTCCACCCGCGCACGCCCCGAACAGA‑CCCGGTAAAACCGGACGAACAACCGCGGCTGGTGCTCGGCAGGCACCCCCGGAcc                                                       >  1:199277/1‑135 (MQ=255)
                                                                            cATGCAACTGCATGATCGACAACCCGCGCGCCACCCCCACCCCGGTCCCGCCCACCCGCCGCGACCGCCCCGACCCTA‑CCGGGAAAAACCGCGCTAACAACCGCGACTGGTGCTCTGCAGGCACACCCGGACCCt                                                     <  6:277617/135‑1 (MQ=255)
                                                                                cAACTGCATGACCGACTTCCCGATCCCCACCCCCACCCCGGCCCCGCCCCCCACCCCCGACCCCCCCGAACCTC‑CCCGTTAAAACCGCTCGAACACCCGCGACTGGTGCTCTGCAGGCACACCCGGACCCTGGtt                                                 <  5:175235/135‑1 (MQ=255)
                                                                                         gATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCGCCAGCCGCGCACGCCCCGAACCTA‑CCCGGTAAAACCGCTCGAACCAGCGCGCCTGGTGCTCTGCAGGCACACCCGGACCCGGGTTGTGCAccg                                         >  2:229107/1‑132 (MQ=255)
                                                                                                                      cGGTCCCGCCCTCCAGCCGCGAACGCCCCGAACCTA‑CCCGGTAAAACCGCTCGAACAAGCGCGACTGGTGCTCTGCAGGCACACCCGGACCCTGGTTCTCCACGGATAGAATGACTTCGCTGCCCGAGCGTTCGa           >  3:145794/1‑135 (MQ=255)
                                                                                                                            cGCCCTCCAGCCGCGCCCGCCCCGAACCTA‑CCCGGTAAAACCGCTCGCACAAGCGCGCCTGGTGCTCTGCAGGCACCCCCGGACCCGGGTTCTCCCCGGATAGAATGACTTCGCTGCCCGAGCGTTCGATGCGCa     >  8:192523/1‑135 (MQ=255)
                                                                                                                               ccTCCCGCCGCGAACGCCCCGAACCTA‑CCCGGTAAAACCGCTCGAACAAGCGCGACTGGTGCTCTGCAGGCACACCCGGACCCTGGTTCTCCACGGATAGAATGACTTCGCTGCCCGAGCGTTCGATGCGCagag  <  7:222308/135‑1 (MQ=255)
                                                                                                                                 tCCAGCCGCGAACGCCCCGAACCTA‑CCCGGTAAAACCGCTCGAACAAGCGCGACTGGTGCTCTGCAGGCACACCCGGACCCTGGTTCTCCACGGATAGAATGa                                <  4:190690/103‑1 (MQ=255)
                                                                                                                                 tCCAGCCGCGAACGCCCCGAACCTA‑CCCGGTAAAACCGCTCGAACAAGCGCGACTGGTGCTCTGCAGGCACACCCGGACCCTGGTTCTCCACGGATAGAATGa                                >  3:190690/1‑103 (MQ=255)
                                                                                                                                    |                                                                                                                                  
GCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTCCAGCCGCGAACGCCCCGAACCTA‑CCCGGTAAAACCGCTCGAACAAGCGCGACTGGTGCTCTGCAGGCACACCCGGACCCTGGTTCTCCACGGATAGAATGACTTCGCTGCCCGAGCGTTCGATGCGCAGAG  >  ACB122/1630408‑1630669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑