Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 2,745,758 A→T 6.9% A86A (GCA→GCT)  czcB_2 → Cobalt‑zinc‑cadmium resistance protein CzcB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1222,745,7580AT 6.9% 176.1 / 4.0 58A86A (GCA→GCT) czcB_2Cobalt‑zinc‑cadmium resistance protein CzcB
Reads supporting (aligned to +/- strand):  ref base A (28/26);  new base T (2/2);  total (30/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.75e-01

GAAGACAGCCACGGCCATGCTGCAGCGCAGGCCGCCGAAGGCCATGCAGAGAAGGAGCACGGCGAAGAAGAAGGCCAGCTGCACCTGTCTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCGGGTGAGATCCGCTTTGACGAGGACCGCACCGCACATGTGGTGCCCCGCGTGCCGGGTGTGGTCGAAGCGGTGCAAGCCGAGCTGGGCCAGGCGGTCAA  >  ACB122/2745627‑2745892
                                                                                                                                   |                                                                                                                                      
gAAGACAGCCACGGCCATGCTGCAGCGCAGGCCGCCGAAGGCCATGCAGAGAAGGAGCACGGCGAAGAAGAAGGCCAGCTGCACCTGTCTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGc                                                                                                                                     >  7:155780/1‑135 (MQ=255)
  agccAGCCACGGCCATGCTGCAGCGCAGGCCGCCGAAGGCCATGCAGAGAAGGAGCACGGCGAAGAAGAAGGCCAGCTGCACCTGTCTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGcc                                                                                                                                    <  6:41682/131‑1 (MQ=255)
      aGCCACGGCCATGCTGCAGCGCAGGCCGCCGAAGGCCATGCAGAGAAGGAGCACGGCGAAGAAGAAGGCCAGCTGCACCTGTCTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCg                                                                                                                                >  6:7556/1‑134 (MQ=255)
      aGCCACGGCCATGCTGCAGCGCAGGCCGCCGAAGGCCATGCAGAGAAGGAGCACGGCGAAGAAGAAGGCCAGCTGCACCTGTCTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGt                                                                                                                               <  8:196206/135‑1 (MQ=255)
         cACGGCCATGCTGCAGCGCAGGCCGCCGAAGGCCATGCAGAGAAGGAGCACGGCGAAGAAGAAGGCCAGCTGCACCTGTCTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGTGAg                                                                                                                            >  7:300219/1‑135 (MQ=255)
          aCGGCCATGCTGCAGCGCAGGCCGCCGAAGGCCATGCAGAGAAGGAGCACGGCGAAGAAGAAGGCCAGCTGCACCTGTCTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGc                                                                                                                           >  5:295151/1‑135 (MQ=255)
                   ctgcAGCGCAGGCCGCCGAAGGCCATGCAGAGAAGGAGCACGGCGAAGAAGAAGGCCAGCTGCACCTGTCTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCg                                                                                                                  >  1:206049/1‑135 (MQ=255)
                       aGCGCAGGCCGCCGAAGGCCATGCAGAGAAGGAGCACGGCGAAGAAGAAGGCCAGCTGCACCTGTCTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGcc                                                                                                                <  5:7556/133‑1 (MQ=255)
                           cAGGCCGCCGAAGGCCATGCAGAGAAGGAGCACGGCGAAGAAGAAGGCCAGCTGCACCTGTCTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGc                                                                                                          >  2:201204/1‑135 (MQ=255)
                                 gccgAAGGCCATGCAGAGAAGGAGCACGGCGAAGAAGAAGGCCAGCTGCACCTGTCTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCg                                                                                                    >  6:57680/1‑135 (MQ=255)
                                      aGGCCATGCAGAGAAGGAGCACGGCGAAGAAGAAGGCCAGCTGCACCTGTCTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCCTCAGCTTCCCGGGTga                                                                                               >  6:260035/1‑135 (MQ=255)
                                      aGGCCATGCAGAGAAGGAGCACGGCGAAGAAGAAGGCCAGCTGCACCTGTCTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCggg                                                                                                  >  7:293255/1‑132 (MQ=255)
                                         ccATGCAGAGAAGGAGCACGGCGAAGAAGAAGGCCAGCTGCACCTGTCTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCGGGTGAGAt                                                                                            <  8:245224/135‑1 (MQ=255)
                                           aTGCAGAGAAGGAGCACGGCGAAGAAGAAGGCCAGCTGCACCTGTCTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCGGGTGAGATcc                                                                                          <  2:176030/135‑1 (MQ=255)
                                                gagaAGGAGCACGGCGAAGAAGAAGGCCAGCTGCACCTGTCTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCGGGTGAGATCCGCttt                                                                                     <  2:253950/135‑1 (MQ=255)
                                                     ggAGCACGGCGAAGAAGAAGGCCAGCTGCACCTGTCTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCGGGTGAGATCCGCTTTGACGa                                                                                <  8:293255/135‑1 (MQ=255)
                                                      gAGCACGGCGAAGAAGAAGGCCAGCTGCACCTGTCTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCGGGTGAGATCCGCTTTGACGa                                                                                >  2:79655/1‑134 (MQ=255)
                                                      gAGCACGGCGAAGAAGAAGGCAAGCTGCACCTGTCTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCAGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCGGGTGAGATCCGCTTTGACGAg                                                                               >  8:162302/1‑135 (MQ=255)
                                                         cACGGCGAAGAAGAAGGCCAGCTGCACCTGTCTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCGGGTGAGATCCGCttt                                                                                     <  3:89779/126‑1 (MQ=255)
                                                         cACGGCGAAGAAGAAGGCCAGCTGCACCTGTCTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCGGGTGAGATCCGCttt                                                                                     >  4:89779/1‑126 (MQ=255)
                                                         cACGGCGAAGAAGAAGGCCAGCTGCACCTGTCTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCGGGTGAGATCCGCTTTGACGAGGac                                                                            >  2:135893/1‑135 (MQ=255)
                                                          aCGGCGAAGAAGAAGGCCAGCTGCACCTGTCTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCGGGTGAGATCCGCTTTGACGAGGacc                                                                           >  3:18583/1‑135 (MQ=255)
                                                           cGGCGAAGAAGAAGGCCAGCTGCACCTGTCTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCTTCAGCTTCCCGGGTGAGATCCGCTTTGACGAGGaccg                                                                          <  8:127340/135‑1 (MQ=255)
                                                           cGGCGAAGAAGAAGGCCAGCTGCACCTGTCTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCGGGTGAGATCCGCTTTGACGAGGaccg                                                                          <  2:275487/135‑1 (MQ=255)
                                                           cGGCGAAGAAGAAGGCCAGCTGCACCTGTCTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCGGGTGAGATCCGCTTTGACGAGGaccg                                                                          <  2:206049/135‑1 (MQ=255)
                                                           cGGCGAAGAAGAAGGCCAGCTGCACCTGTCTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCGGGTGAGATCCGCTTTGACGAGGaccg                                                                          <  1:9787/135‑1 (MQ=255)
                                                           cGGCGAAGAAGAAGGCCAGCTGCACCTGTCTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCGGGTGAGATCCGCTTTGACGAGGaccg                                                                          <  1:4415/135‑1 (MQ=255)
                                                              cGAAGAAGAAGGCCAGCTGCACCTGTCTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCGGGTGAGATCCGCTTTGACGAGGaccgcac                                                                       >  7:87788/1‑135 (MQ=255)
                                                              cGAAGAAGAAGGCCAGCTGCACCTGTCTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCGGGTGAGATCCGCTTTGACGAGGaccgcac                                                                       >  4:91081/1‑135 (MQ=255)
                                                                aagaagacgGCCAGCTGCACCTGTCTATCGCGCAGATCGAATCGGCGGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCGGGTGAGATCCGCTTTGACGAGGaccgcaccg                                                                     <  6:95367/135‑1 (MQ=255)
                                                                aagaagaagGCCAGCTGCACCTGTCTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCTGGcc                                                                                                                                    <  2:202247/72‑1 (MQ=255)
                                                                aagaagaagGCCAGCTGCACCTGTCTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCTGGcc                                                                                                                                    >  1:202247/1‑72 (MQ=255)
                                                                aagaagaagGCCAGCTGCACCTGTCTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCTGGcc                                                                                                                                    >  1:206582/1‑72 (MQ=255)
                                                                aagaagaagGCCAGCTGCACCTGTCTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCTGGcc                                                                                                                                    <  2:206582/72‑1 (MQ=255)
                                                                   aagaagGCCAGCTGCACCTGTCTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACAGCCATCAGCTTCCCGGGTGAGATCCGCTTTGACGAGGACcgcaccgcac                                                                  <  8:87788/135‑1 (MQ=255)
                                                                         gCCAGCTGCACCTGTCTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCGGGTGAGATCCGCTTTGACGAGGACCGCACCGCACATgtgg                                                            >  6:133929/1‑135 (MQ=255)
                                                                             gCTGCACCTGTCTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCGGGTGAGATCCGCTTTGACGAGGACCGCACCGCACATGTGGTGcc                                                        <  3:120172/135‑1 (MQ=255)
                                                                                gCACCTGTCTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCGGGTGAGATCCGCTTTGACGAGGACCGCACCGCACATGTGGTGCCCcg                                                     >  4:3260/1‑135 (MQ=255)
                                                                                        cTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCGGGTGAGATCCGCTTTGACGAGGACCGCACCGCACATGTGGTGCCCCGCGTGCCg                                              <  8:155780/134‑1 (MQ=255)
                                                                                        cTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCGGGTGAGATCCGCTTTGACGAGGACCGCACCGCACATGTGGTGCCCCGCGTGCCg                                              <  6:4007/134‑1 (MQ=255)
                                                                                        cTATCGCGCAGATCGAATCGGCGGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCGGGTGAGATCCGCTTTGACGACGACCGCACCGCACATGTGGTGCCCCGCGTGCCg                                              <  2:216157/134‑1 (MQ=255)
                                                                                        cTAGCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCGGGTGAGATCCGCTTTGACGAGGACCGCACCGCACATGTGGTGCCCCGCGTGCCg                                              <  6:46424/134‑1 (MQ=255)
                                                                                          aTCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCGGGTGAGATCCGCTTTGACGAGGACCGCACCGCACATGTGGTGCCCCGCGTGCCGGgt                                           >  6:9574/1‑135 (MQ=255)
                                                                                                 aGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGcc                                                                                                                                    >  1:194268/1‑39 (MQ=255)
                                                                                                 aGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGcc                                                                                                                                    <  2:194268/39‑1 (MQ=255)
                                                                                                 aGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCGGGTGAGATCCGCGTTGACGAGGACCGCACCGCAAATGTGGTGCCCCGCGTGCCGGGTGTGGTCg                                    <  6:159518/135‑1 (MQ=255)
                                                                                                   aTCGAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCGGGTGAGATCCGCTTTGACGAGGACCGCACCGCACATGTGGTGCCCCGCGTGCCGGGTGTGGTCGaa                                  >  7:39256/1‑135 (MQ=255)
                                                                                                      gAATCGGCTGGCATACACCTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCGGGTGAGATCCGCTTTGACGAGGACCTCACCGCACATGTGGTGCCCCGCGTGCCg                                              >  5:27300/1‑120 (MQ=255)
                                                                                                      gAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCt                                                                                                         >  4:186430/1‑61 (MQ=255)
                                                                                                      gAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCt                                                                                                         <  3:186430/61‑1 (MQ=255)
                                                                                                      gAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCGGGTGAGATCCGCTTTGACGAGGACCGCACCGCACATGTGGTGCCCCGCGTGCCg                                              <  6:27300/120‑1 (MQ=255)
                                                                                                                gCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCGGGTGAGATCCGCTTTGACGAGGaccgca                                                                        <  2:263301/84‑1 (MQ=255)
                                                                                                                gCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCGGGTGAGATCCGCTTTGACGAGGaccgca                                                                        >  1:263301/1‑84 (MQ=255)
                                                                                                                gCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCGGGTGAGATCCGCTTTGACGAGGACCGCACCGCACATGTGGTGCCCCGCGTGCCGGGTGTGGTCGAAGCGGTGCAAGc                       >  1:114858/1‑133 (MQ=255)
                                                                                                                              gCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCGGGTGAGATCCGCTTTGACGAGGACCGCACCGCACATGTGGTGCCCCGCGTGCCGGGTGTGGTCGAAGCGGTGCAAGCCGAGCTGGGCCAGGCg       >  3:102415/1‑135 (MQ=255)
                                                                                                                              gCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCGGGTGAGATCCGCTTTGACGAGGACCGCACCGCACATGTGGTGCCCCGCGTGCCGGGTGTGGTCGAAGCGGTGCAAGCCGAGCTGGGCCAGGCg       >  6:292350/1‑135 (MQ=255)
                                                                                                                              gCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCGTCCCGGGTGAGATCCGCTTTGACGAGGACCGCACCGCACAGGTGGTGCCCCGCGTGCCGGGTGTGGTCGAAGCGGTGCAAGCCGAGCTGGGCCAGGCg       >  3:97690/1‑135 (MQ=255)
                                                                                                                                 gCAGGCCCCCGTGCGCTGGGCACCGCCATCAGCTTCCCGGGTGAGATCCGCTTTGACGAGGACCCCCCCGCACATGTGGTGCCCCGCGGGCCGGGTGTGGGCGAAGCGGTGCAAGCCGCGCGGGGCCAGGCGGTc    <  4:18583/135‑1 (MQ=255)
                                                                                                                                   aGGCCCGCGTGAGCTGGGCACGGCCATCAGCTTCCCGGGTGAGATCCGCTTTGACGAGGACCGCACCGCACATGTGGTGCCCCGCGTGCCGGGTGTGGTCGAAGCGGTGCAAGCCGAGCTGGGCCAGGCGGTCaa  <  3:91081/135‑1 (MQ=255)
                                                                                                                                   aGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCGGGTGAGATCCGCTTTGACGAGGACCGCACCGCACATGTGGTGcc                                                        >  4:188814/1‑81 (MQ=255)
                                                                                                                                   aGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCGGGTGAGATCCGCTTTGACGAGGACCGCACCGCACATGTGGTGcc                                                        <  3:188814/81‑1 (MQ=255)
                                                                                                                                   aGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCGGGTGAGATCCGCTTTGACGAGGACCGCACCGCACATGGGGTGCCCCGCGTGCCGGGTGTGGTCGAAGCGGTGCAAGCCGAGCTGGGCCAGGCGGTCaa  <  5:151400/135‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                      
GAAGACAGCCACGGCCATGCTGCAGCGCAGGCCGCCGAAGGCCATGCAGAGAAGGAGCACGGCGAAGAAGAAGGCCAGCTGCACCTGTCTATCGCGCAGATCGAATCGGCTGGCATACAACTGGCTGCCGCAGGCCCCCGTGAGCTGGGCACCGCCATCAGCTTCCCGGGTGAGATCCGCTTTGACGAGGACCGCACCGCACATGTGGTGCCCCGCGTGCCGGGTGTGGTCGAAGCGGTGCAAGCCGAGCTGGGCCAGGCGGTCAA  >  ACB122/2745627‑2745892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑