| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 5,688,615 | T→G | 56.2% | R228R (CGT→CGG) | pyrC' → | Dihydroorotase‑like protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 5,688,615 | 0 | T | G | 56.2% | 1.2 / 20.0 | 33 | R228R (CGT→CGG) | pyrC' | Dihydroorotase‑like protein |
| Reads supporting (aligned to +/- strand): ref base T (4/10); new base G (9/10); total (13/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.10e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.11e-02 | |||||||||||
ACCTGGCCCAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTT > ACB122/5688485‑5688746 | aCCTGGCCCAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTgcgc < 8:16516/135‑1 (MQ=255) ccTGGCCCAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTgcgcc > 4:60583/1‑134 (MQ=255) cccAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTc < 7:8470/134‑1 (MQ=255) ccAGGGAGGCCTGGCCCACGAAGGTGCCAAGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAACCCGTGGCCCTGGCGCGCAACCTGCTGCTGGTCGAACAGAGCGGCGGGGGGGCGCCCTTccg > 4:108293/1‑133 (MQ=255) cggcCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCCTCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGCGCGGCGTGGGGGCGCCCTTCAGCCAGa > 8:192264/1‑134 (MQ=255) tGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGGGCGGGGGCCCGTCAGCCCCATAACCa > 4:98796/1‑135 (MQ=255) aGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCCCCCCCGCCCGTgg > 2:56449/1‑135 (MQ=255) tGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGGGCGGCCGTCCGCCACATCACCCGCCCCCCAGGCGCCCGGc > 1:88799/1‑135 (MQ=255) ccTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGGGCGGGGGCCCGTCAGCCCCCTCACCCCCGCCCCGGGCGCGCGGGGGCTCgcccc > 7:34190/1‑134 (MQ=255) cgcggccggccggcCAGCCCGCCGCCCGCCTAAACGGGGGCCCGGGCGCGCAACCTGCGGGGGGTGCAACAGCGCGGCGGGCGGGCGCACTTCAGCATGACCCCCCGCGCCCGGGGCGCGCGGCTGATCgcccag < 4:87631/133‑1 (MQ=255) cgcggccggcGGGCCAGCCGGTAGAGCGCCGAAACCGGGGCCGGGGGGCGCACCCTGGTGGGGGGGCACCAGCGCGGCGTGGGTGCGCACTCCCGCCTCAACCCCCGCGCCCGGGGCGCGCGGCTGATCgcccag < 4:109173/133‑1 (MQ=255) gCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCTTGAGCCCGATCACCCGCGCCCGGGGCGCGCGGCGGGTCgccccggccc > 8:191776/1‑135 (MQ=255) ggccgcccGGCGAGCGCCGAACCGGGGCCCCGGGCGCGCACCGTGGTGCGGGGGTACCCGCGGGGCGGGCGGGCGCAGTCCCGCCAGAGCCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTg < 2:103506/130‑1 (MQ=255) gagCGCCGAAACCGTGGCCCGGGCGCGCAACCGGGTGCGGGGGGAACAGAGCGGGGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGt < 8:205090/135‑1 (MQ=255) cgcgGAACCCGGGGCCGTGGCGCGCAACCTGCTGGGGGTGTAACTGAGCGGGGTGCGGGCGCAGTGCAGCCAGTTCACCAGGGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc < 6:140821/135‑1 (MQ=255) cgcCTAAACCGTGGCCGTGGCGCGCAACCTGGTGCTGGTGGAACATAGGGGCGTGCGTGCGCACTTCAGCATGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGt < 8:205089/132‑1 (MQ=255) ccccGAACCCGGGGCCGGGGGGCGCAACGTGCTGCTGGGGCAACTGCGGGGGGGGCGGGGGCACTGCCCCCACAGCCCCGGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc < 2:78232/133‑1 (MQ=255) ccGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGGGGACCAGAGCGGCGTGCGTGCGCACTTCCGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccg < 4:143303/135‑1 (MQ=255) cGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGGTGGGACTTGAGCCCGCGACCCCACGCCCCTGGCGCGCGGCGGGTCGCCCCGGCCCCGGGCGTGGGGCGGGCGGtttacgc > 2:231691/1‑129 (MQ=255) cGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGGGGGCCCATACGCCCCATCACCCCCCCCCCGGGGGCGCCGCTGCTAGCCCACGCCCCCGCCCTGGGCCGGCCCGTGAccgc > 8:41911/1‑135 (MQ=255) cGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGCGCACTTGAGCCCGATAACCCGCGCCCCTGGCGCGCCGCGGATCGCCCCCGCCCCCGCCGTGGGCCTGCCGGGAAccgc > 2:198935/1‑135 (MQ=255) cGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGCGCACTTCCGCCAGATCACCCGCGCCCGTGGGGCGCGGCGGCTCGCCCCGGCCCCGGCCGGGGGCCTGGCGGTAAccgc > 6:148736/1‑135 (MQ=255) cgggggCCCGGGCGCGCAACCTGCTGCTGGTGCAACAGAGCGGCGTGCGGGCGCACTTCAGCCAGACCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATg < 7:199312/131‑1 (MQ=255) ggggCCGTGGCGCTCACCCTGCGGGTGGGGCAACAGGGGGGCGGGCGGGCGCACTACCCCCAGAACCCCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa < 8:117575/133‑1 (MQ=255) ggggCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGGGGCGGGCGTGCGCACTTCAGCCAGATCACCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa < 7:191776/133‑1 (MQ=255) ggggCCCGGGCGCGAACCCTGCGGGGGGGGCAACAGAGCGGCGGGCGGGCGCACTACCGCCAGAGCACCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa < 8:91759/133‑1 (MQ=255) gcggCCCGGGCCGGCAACCTCTTGGTGGTGAAACCTTGCGGGGTGCTTCCGCACTACAGCCTGATCCACCGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa < 7:238635/133‑1 (MQ=255) gTGGCCCTGGGGCGCAACCTGCTGGTGTTGCCACTGAGCGGCGTGCGGGCGAAATTCTGCAAGATCACCAGCGCGCGTGGCGCGCGGCTGATCGCCCAGGCCCAGTCCTTGGGCCTGCCGGTAACCGCCGATGTa < 8:87698/135‑1 (MQ=255) gTGGCCCGGGCGCGCAACCTGTTGCTGGTGGAACAGAGCGGGTGGCGTGGTCAGTTCAGCCAGATAACGAGCGCCCGTGGAGCGCGGCTGATCGCCGAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa < 2:85170/135‑1 (MQ=255) cccGGGCGCTCACCGGGCTGCGGGGGCAACAGCGCGGGGGGGGGGCGCACTCCACCCATACCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAgcgc < 3:253510/135‑1 (MQ=255) gcgcAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCCGCTGATCGCCCACGCCCCGGCCTTGGGCCTGCCGGGAACCGCCGATGTAGCGCGGTACCa > 7:75481/1‑135 (MQ=255) cgcggggGGCGTGCGCACTGCGGCCCGACCCCCCGGGCGGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTg < 7:152514/134‑1 (MQ=255) ggcggggcgtgcgAAATCCAGCCCTAACCCCTGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg < 6:4533/129‑1 (MQ=255) cggcggGCGGGCGCACTCCAGCCAGAGCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg < 8:172778/135‑1 (MQ=255) gcgggcggGCGCACTCCCCCCAGACCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGcgcg < 8:17566/135‑1 (MQ=255) ggcgtgcgCACTTCTGCCACAGCACCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt < 2:88799/134‑1 (MQ=255) ggcgtgcgCACTTCAGCCAGAACCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt < 3:60583/134‑1 (MQ=255) gcgtgcgCACTTCAGCCAGATCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGtt < 2:15784/135‑1 (MQ=255) | ACCTGGCCCAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTT > ACB122/5688485‑5688746 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |