Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB122 4,760,690 G→C A24P (GCA→CCA)  vanB → vanillate O‑demethylase oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1224,760,6900GC97.7% 301.9 / ‑1.4 85A24P (GCA→CCA) vanBvanillate O‑demethylase oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base C (56/27);  total (58/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.22e-01

AGCGTGCGCCGCAGCCGCAACTGATCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTA  >  ACB122/4760559‑4760818
                                                                                                                                   |                                                                                                                                
agCGTGCGCCGCAGCCGCAACTGATCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTACCGATGAAGCGCAGGGTATCTGCAGCGTCGAGCTGGCCGCGCCAGag                                                                                                                             >  8:288056/1‑136 (MQ=255)
agCGTGCGCCGCAGCCGCAACTGATCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGAt                                                                                                                             >  4:91545/1‑137 (MQ=255)
   gTGCGCCGCAGCCGCAACTGATCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGc                                                                                                                          <  8:84504/137‑1 (MQ=255)
    ggcgcCGCAGCCGCAACTGATCCCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCa                                                                                                                         <  7:384140/136‑1 (MQ=255)
       gccgcagccgcaACTGATCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGcc                                                                                                                      >  4:307568/1‑137 (MQ=255)
       gccgcagccgcaACTGATCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGcc                                                                                                                      >  8:60842/1‑137 (MQ=255)
       gccgcagccgcaACTGATCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGcc                                                                                                                      >  4:350634/1‑137 (MQ=255)
       gccgcagccgcaACTGATCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGcc                                                                                                                      >  4:363451/1‑137 (MQ=255)
       gccgcagccgcaACTGATCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGcc                                                                                                                      >  2:188740/1‑137 (MQ=255)
       gccgcagccgcaACTGATCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGcc                                                                                                                      >  7:225090/1‑137 (MQ=255)
       gccgcagccgcaACTGATCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGcc                                                                                                                      >  5:65219/1‑137 (MQ=255)
       gccgcagccgcaACTGATCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGcc                                                                                                                      >  5:87236/1‑137 (MQ=255)
       gccgcagccgcaACTGATCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGcc                                                                                                                      >  6:31880/1‑137 (MQ=255)
       gccgcagccgcaACTGATCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCAGCGCCAGATGGCAGcc                                                                                                                      >  6:229296/1‑137 (MQ=255)
            agccgcaACTGATCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCctgctg                                                                                                                 <  1:103790/137‑1 (MQ=255)
            agccgcaACTGATCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCctgctg                                                                                                                 <  4:299647/137‑1 (MQ=255)
            agccgcaACTGATCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCctgctg                                                                                                                 <  3:269048/137‑1 (MQ=255)
                      gATCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTcc                                                                                                       >  8:262560/1‑136 (MQ=255)
                      gATCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTcc                                                                                                       >  8:345241/1‑136 (MQ=255)
                         cGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAgcg                                                                                                    <  1:188740/137‑1 (MQ=255)
                         cGCCACCAGCGCCAACCCTGCCGCAGGAACAGCCGACATGATCGCTGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAgcg                                                                                                    <  5:191245/137‑1 (MQ=255)
                           ccaccaGCGCCAACCCTGCCTCAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGAAGGCAGCCTGCTGCCGGCGTTCAGCGcc                                                                                                  >  6:347519/1‑137 (MQ=255)
                                   gcCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCa                                                                                          <  6:23742/137‑1 (MQ=255)
                                     cAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCata                                                                                        <  4:73140/137‑1 (MQ=255)
                                         cctgcctgAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGa                                                                                    >  7:12799/1‑137 (MQ=255)
                                         cctgcctgAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGa                                                                                    >  3:359345/1‑137 (MQ=255)
                                         cctgcctgAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGa                                                                                    >  7:7413/1‑137 (MQ=255)
                                              atgAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGc                                                                               <  1:166752/136‑1 (MQ=255)
                                                gAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCgg                                                                                                >  3:45646/1‑118 (MQ=255)
                                                gAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCgg                                                                                                <  4:45646/118‑1 (MQ=255)
                                                gAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCAc                                                                             >  7:195781/1‑137 (MQ=255)
                                                gAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCAc                                                                             >  7:276415/1‑137 (MQ=255)
                                                gAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCAc                                                                             >  2:89817/1‑137 (MQ=255)
                                                  ggAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCt                                                                           <  3:106026/137‑1 (MQ=255)
                                                   gAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTg                                                                          >  3:323899/1‑137 (MQ=255)
                                                         ccGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGGTGCCGGCGGTCAGCGCCGGCGCCCATATAGACGTGGACCTGCCCgcc                                                                    >  7:87669/1‑135 (MQ=255)
                                                         ccGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGAc                                                                    >  8:2768/1‑137 (MQ=255)
                                                          cGACATGATCGATGCCGTAGTGGTATCCCGTACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCAGCCCCAccg                                                                  >  7:153959/1‑135 (MQ=255)
                                                            aCATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACggg                                                                 >  3:318114/1‑137 (MQ=255)
                                                                 ctCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGt                                                            <  6:52676/136‑1 (MQ=255)
                                                                 cgcgctGCCGTCGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTGCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGt                                                            <  8:24552/132‑1 (MQ=255)
                                                                 aTCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGt                                                            <  4:323899/137‑1 (MQ=255)
                                                                    gATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCCCCAGCCCCACGGGCAGGTgcg                                                         >  4:3462/1‑137 (MQ=255)
                                                                        ccGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAg                                                     >  2:307991/1‑137 (MQ=255)
                                                                        ccGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGCCGCCCATATCGACGTGCACCTCCCCCCCCGGCTGGGGCGCCaa                                                     >  4:94834/1‑136 (MQ=255)
                                                                         cGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGt                                                    <  6:87236/137‑1 (MQ=255)
                                                                         cGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGt                                                    <  3:45629/137‑1 (MQ=255)
                                                                         cGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCCCCGGGCAGGTGCGCCact                                                    >  1:78543/1‑135 (MQ=255)
                                                                           tAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTAt                                                  <  6:334611/137‑1 (MQ=255)
                                                                            aGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTAtt                                                 <  1:241135/137‑1 (MQ=255)
                                                                                      cGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCCCCTGCCCGACCGGCTGGTGCGCCAGTATTTCCTGTGCaa                                       >  3:366458/1‑137 (MQ=255)
                                                                                      cGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCCCCTGCCCGACCGGCTGGTGCGCCACTATTAGCTGTGCaa                                       >  8:60923/1‑137 (MQ=255)
                                                                                      cGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTTCCCGACGGGCTGGTGCGCCAGTATTAGCTGTGCaa                                       >  7:79845/1‑137 (MQ=255)
                                                                                      cGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCaa                                       >  4:221377/1‑137 (MQ=255)
                                                                                      cGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCaa                                       >  4:295402/1‑137 (MQ=255)
                                                                                      cGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCaa                                       >  7:289944/1‑137 (MQ=255)
                                                                                      cGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCaa                                       >  4:239230/1‑137 (MQ=255)
                                                                                      cGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCaa                                       >  8:366725/1‑137 (MQ=255)
                                                                                      cGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTAGCTGTGCaa                                       >  3:95397/1‑137 (MQ=255)
                                                                                      cGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTAGCTGTGCaa                                       >  3:288884/1‑137 (MQ=255)
                                                                                      cGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACCGGCTGGGTCGCCAGTATTCGCGGTTCaa                                       >  6:226804/1‑137 (MQ=255)
                                                                                           cGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTTCCGGCGTTCAGCGCCGGAGCCCATATCGACGTGCACCAGCCAGAACGGCTGGTGCGCCCGTATTCGCTGTGCAacaacc                                  >  8:1464/1‑137 (MQ=255)
                                                                                           cGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCCCCTGCCCGCCGGGGGGGGGGGCCCGTTTTGGCTGTGCAaccccc                                  >  1:314198/1‑137 (MQ=255)
                                                                                           cGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAaccacc                                  >  4:26220/1‑137 (MQ=255)
                                                                                              tGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCCCCTGCCCGACGGGCTGGTGCGCCAGTATTAGCTGTGCAACCAccccc                               >  4:277609/1‑136 (MQ=255)
                                                                                              tGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCg                               <  8:195781/137‑1 (MQ=255)
                                                                                              tGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCg                               >  6:319884/1‑137 (MQ=255)
                                                                                              tGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGAACGGGGGGTGGGGCCGGTTTCGATGTGCACCCAccccc                               >  4:256651/1‑136 (MQ=255)
                                                                                                aaGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCgaa                             >  7:128487/1‑137 (MQ=255)
                                                                                                aaGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCgaa                             <  5:11392/137‑1 (MQ=255)
                                                                                                   cgcAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCCCCTGCCCGACGGGCTGGTGGGGCAGGATTAGCTGTGCAAACAccccccaaaa                          >  5:8814/1‑131 (MQ=255)
                                                                                                   cgcAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCgaagaa                          >  6:188656/1‑137 (MQ=255)
                                                                                                    gcAGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAAc                         >  8:285427/1‑137 (MQ=255)
                                                                                                      aGGGTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGc                       <  3:307568/137‑1 (MQ=255)
                                                                                                        ggTATCTGCAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCa                     <  2:138564/137‑1 (MQ=255)
                                                                                                                cAGCTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATc             <  7:24728/137‑1 (MQ=255)
                                                                                                                cAGCTTCCAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGCCGTTCCGCGCCGGAGCCCATATAGACGTGCACCTGCCCGACCGGCTGGTGCGCCACAAATCGCAGTGCAACCCCCACCAAGAACCCCAGCCCTAta             >  2:185637/1‑136 (MQ=255)
                                                                                                                   cTTCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGa          <  3:277609/137‑1 (MQ=255)
                                                                                                                     tCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGGCCCTGGCCGCCGGGCTGGGGGGGCAGTATTAGCTGTGGAACCACCCCCAAGAAAGCCCTCGCGATcagctt        >  4:199164/1‑132 (MQ=255)
                                                                                                                     tCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCGGGGGCGCCACTATTCGCGGTGCAACCCCCCCGCAGAACGCCCTCCCTATCTGAtt        >  6:324523/1‑137 (MQ=255)
                                                                                                                     tCGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGGGCCTGCCCGACGGGCTGGTGCGCCAGTATTCGGTGTGCAAACACCCCGAAGAACGCCATCGGTATCTGAtt        >  4:337499/1‑137 (MQ=255)
                                                                                                                      cGAGCTGGCCGCGCCTGTTGGCAGGCTGCTTCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTg       <  3:94834/137‑1 (MQ=255)
                                                                                                                      cGAGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTg       <  3:295402/137‑1 (MQ=255)
                                                                                                                        aGCTGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCCACCGGCGGGTGGGCCACGATTAGCGGTGCCACCACCCCCCAGCACGCCATCGCGATCTGATTggg     >  6:11227/1‑136 (MQ=255)
                                                                                                                           tGGCCGCGCCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCCCCCCGAAGAACGCCATCGCTATCTGATTGGCGtt  >  6:141439/1‑136 (MQ=255)
                                                                                                                                   |                                                                                                                                
AGCGTGCGCCGCAGCCGCAACTGATCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTA  >  ACB122/4760559‑4760818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: