Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB122 690,144 A→G intergenic (+21/‑2438) bauC → / → PP_RS08750 Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB122690,1440AG91.7% 14.1 / ‑6.4 12intergenic (+21/‑2438)bauC/PP_RS08750Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (11/0);  minor base C (1/0);  total (12/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCGGATGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTGGGGGTTGGTTGTTGCTGTTGGGGAGGAACTGT  >  ACB122/690021‑690205
                                                                                                                            |                                                                
cccGGATGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCGGCAGGcca                                                    >  2:115682/1‑136 (MQ=255)
       ggTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCGGCCGGGCGccccccc                                             >  3:33700/1‑134 (MQ=255)
       ggTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCCGCAGgccgcgccgcg                                             >  8:196498/1‑136 (MQ=255)
                             gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGGCGGCCGCCGGCCCCCCTTGGTTTTTTGGGGTGtgttgt                      >  4:337830/1‑137 (MQ=255)
                             gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCGGGCGGGCGGCCCCCCGGGGTTTTTGGGgggtggggtt                      >  4:299161/1‑133 (MQ=255)
                             gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGCCGGCAGGCCTCCCCCCGGTGGTTTTTGGGGGTGggtgtt                     >  6:82961/1‑137 (MQ=255)
                               aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCGGCCGGCCGCCCCGCGGTTTTTTTTGGGGGTGgttgtttg                   >  6:193452/1‑135 (MQ=255)
                               aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGCGGCCCGCATGGCGCCCCCCCGTTTTTTTTGGgggtgggtgtttg                    >  8:376710/1‑132 (MQ=255)
                                         cccAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCGGCTGGGCGGCCCGCGGTGTTTTTGTGGGGTGGTTGGTGGTGGTgggggg         >  3:251500/1‑135 (MQ=255)
                                               gTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCGGGAGGCGGCCCCCCGTTTTTTTTTTGGGGTGGGTGTTGGTGGTGGGGTGGGACTg   >  8:200156/1‑137 (MQ=255)
                                                 tGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCGGGCGGCCGCCCCGCGTGGTTTTTTGGGGGGTGGTGGTTGGGTTTGGgggggacagtt  >  8:89707/1‑132 (MQ=255)
                                                 tGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCGGGCGGCCGCCCCCCGGTTTTTTTGTGGGGTTGTTTTTTGGGGTTGgggggggaggtt  >  7:40582/1‑131 (MQ=255)
                                                                                                                            |                                                                
CCCGGATGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTGGGGGTTGGTTGTTGCTGTTGGGGAGGAACTGT  >  ACB122/690021‑690205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: