Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB122	690,150	T→G	intergenic (+27/‑2432)	bauC → / → PP_RS08750	Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	690,150	0	T	G	100.0%	10.6 / NA	12	intergenic (+27/‑2432)	bauC/PP_RS08750	Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (12/0);  total (12/0)

CCCGGATGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGCCGTCA‑GGCCTCCCCGCTTTG‑TTTTTG‑GGGGTTGGTTGTTGCTGTTGGGGAGGAACTGT  >  ACB122/690021‑690205                                                                                                                                   |                                                           cccGGATGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGCA‑GGcca                                                    >  2:115682/1‑136 (MQ=255)        ggTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGCC‑GGGCGccccccc                                             >  3:33700/1‑134 (MQ=255)        ggTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCCGCA‑Ggccgcgccgcg                                             >  8:196498/1‑136 (MQ=255)                              gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGGCGGCC‑GCCGGCCCCCCTTGG‑TTTTTT‑GGGGTGtgttgt                      >  4:337830/1‑137 (MQ=255)                              gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCGGGCG‑GGCGGCCCCCCGGGG‑TTTTTG‑GGgggtggggtt                      >  4:299161/1‑133 (MQ=255)                              gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGCCGGCA‑GGCCTCCCCCCGGTG‑GTTTTT‑GGGGGTGggtgtt                     >  6:82961/1‑137 (MQ=255)                                aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGCC‑GGCCGCCCCGCGGTT‑TTTTTT‑GGGGGTGgttgtttg                   >  6:193452/1‑135 (MQ=255)                                aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGCGGCCCGCATGGCGCCCCCCCGTTT‑TTTTTG‑Ggggtgggtgtttg                    >  8:376710/1‑132 (MQ=255)                                          cccAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGCT‑GGGCGGCCCGCGGTG‑TTTTTG‑TGGGGTGGTTGGTGGTGGTgggggg         >  3:251500/1‑135 (MQ=255)                                                gTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGGA‑GGCGGCCCCCCGTTT‑TTTTTT‑TGGGGTGGGTGTTGGTGGTGGGGTGGGACTg   >  8:200156/1‑137 (MQ=255)                                                  tGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGGC‑GGCCGCCCCGCGTGGTTTTTTG‑GGGGGTGGTGGTTGGGTTTGGgggggacagtt  >  8:89707/1‑132 (MQ=255)                                                  tGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGGC‑GGCCGCCCCCCGGTT‑TTTTTGTGGGGTTGTTTTTTGGGGTTGgggggggaggtt  >  7:40582/1‑131 (MQ=255)                                                                                                                                   |                                                           CCCGGATGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGCCGTCA‑GGCCTCCCCGCTTTG‑TTTTTG‑GGGGTTGGTTGTTGCTGTTGGGGAGGAACTGT  >  ACB122/690021‑690205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑