Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,094,8080AC51.3% ‑1.4 / 18.1 43L186R (CTG→CGG) CJ019_01995hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (14/5);  new base C (10/10);  total (24/19)
Fisher's exact test for biased strand distribution p-value = 1.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.15e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GGGGCCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCAAGAA  >  ACB143/2094682‑2094938
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ggggCCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTAGAAGGCCACgcgcc                                                                                                                              >  1:103473/1‑132 (MQ=255)
     cATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGCAGGCCCGGCGCGCCTGGcc                                                                                                                       >  6:79263/1‑135 (MQ=255)
       tGCGGGCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCgg                                                                                                                     <  7:25329/135‑1 (MQ=255)
               aGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCCGCGCGCCAGGCCGGGCGGGccg                                                                                                             >  2:33058/1‑134 (MQ=255)
               aGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCACGCGCCCCcgggcgggcggggcg                                                                                                             >  5:27202/1‑132 (MQ=255)
                         cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCTGGGCGGGGGGGCCGTTTTGGAggg                                                                                                   >  2:229664/1‑135 (MQ=255)
                         cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCGGGCCGGGCTGGCCGTTTTGCCGGGc                                                                                                  >  4:300653/1‑136 (MQ=255)
                         cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCGTTTTGCAggg                                                                                                   >  2:135884/1‑135 (MQ=255)
                         cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCCAAGGCCAGGCGCGCCGGGCCGGGCGGGCCGTTTTGCAgggg                                                                                                  >  4:199588/1‑135 (MQ=255)
                         cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCATGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCAGCGCGGGGGGGCCGTTTTGAAggc                                                                                                   >  5:108003/1‑134 (MQ=255)
                         cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCGTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCCGCGCGCCGGGCCGGGCGGGGCGTTTTTGAgggg                                                                                                  >  3:351913/1‑135 (MQ=255)
                              gCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCCGCGCGCCGGGCCGGGCGGGGCGTTTTGGAGGGCCGGt                                                                                              >  8:39718/1‑135 (MQ=255)
                               cccGGCACACCATTTTCACAATATCCTTCACCGGCTATACAAACCCCTGCGGCCCCGGCGCCTGGCCAAAATCCACCACGCCGCGCTCCACGGCCCGGCGCCCCGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTg                                                                                            <  4:32307/136‑1 (MQ=255)
                                      cAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCGGGCCGGGCGGGCCGTTTTTGAGGGGCGGTTGGGCGAg                                                                                      >  7:88815/1‑135 (MQ=255)
                                             ttCACACGATCCGTCGGCTGCTCTCAATCCCCGTGCGGCGCCGGGGGCTGGCGAAAATCCGCCCCGCCGCGCTCGACGCCCCGGGGCGCGTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGgttgtt                                                                                <  6:377674/134‑1 (MQ=255)
                                             tgctcAATATACGGCCGCTGCTCTACATCCACCTGCGGCGCGGGCTGCTGCCCCAAAAACGCCACGCCGCGCTCGACGGCCCGGCGCGCCGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGgttgtt                                                                                <  7:353405/132‑1 (MQ=255)
                                               ctcAAGATACGTCCGCTGCTCTTCATACACCTGCGGCGCCGGCTGCTTGCTCAAATACTGCCCGCCGCGCTCGATGGCCAGGCGCGCCGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTga                                                                              <  6:178697/134‑1 (MQ=255)
                                                 cAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCAGGCCGGGCTGGCCTTTTTGCAGGGCCGGTTGGCCGAGGTTGTTgaag                                                                            >  5:188397/1‑134 (MQ=255)
                                                          tCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCCGCGCGCCTGGGCGGGGGGGGCGTTTTGGAGGGCCTGGGTGCCGGGGTTGTTGAATGACTatatat                                                                  >  6:215403/1‑135 (MQ=255)
                                                               tgctCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGGCGGGCTGGCCTTTTTTGAGGGCCTGTTGGCCGAGGTTGGTGAAGAACACTTTATgcag                                                              >  3:86592/1‑134 (MQ=255)
                                                                 ctctTCATACACCTTCGGCTCCGGCAGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCGCGCCTTTTTGCAGGGCCTGTTGTCCGAGGTGGTTGAACAACACTAAATgcagca                                                            >  2:342550/1‑134 (MQ=255)
                                                                  tctTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGTGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATgcagcag                                                           <  7:238001/134‑1 (MQ=255)
                                                                  tctTCATACACCTTCGGCGCCGGCTGCTGGCTCAAAAACTGCCCGCCGCGCTCGACGGCCCGGCGCGCCGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATgcagcag                                                           <  8:88815/134‑1 (MQ=255)
                                                                           cccTTCGGCTCCGGGTGTTTGCGAAAATAATCCACGCGGCGCTCTAAGGCCTGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGata                                                 <  2:97032/134‑1 (MQ=255)
                                                                                 ggCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCGTTTTGCAGGGCCAGGTGGCCGCCGTTGTTGAAGAACAAGATATGCATCAGCCACAGGATATGgggg                                           >  2:15169/1‑135 (MQ=255)
                                                                                   cTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGGTGAAGAACTCTATATTCAGCAGCACCAGGAAATGGCGGAt                                         >  7:94435/1‑135 (MQ=255)
                                                                                   cTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCCGGTGGGCGAGGTTGTTGTATAAATCTATATTCAGCAGCCCCAGGATATGGCGGAt                                         >  6:67424/1‑135 (MQ=255)
                                                                                     ccGGCTGCTTGCGCAAAACCTCCCCGCCGCGCTCGACGGCCCGGCGCGCCGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAttt                                       <  7:69079/135‑1 (MQ=255)
                                                                                          tgctTGCGAAAATACTGCCCCCCGCGCTCGACGGCCCGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTg                                  <  6:96509/135‑1 (MQ=255)
                                                                                          ggcgtGCGAAAACACTCCCCGCCGCGCTCGAGGGCCCGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTg                                  <  7:375071/131‑1 (MQ=255)
                                                                                                cgcAATAACTGCACGCCGCGGGGCTCGGCCGGGCGGGCCGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCaa                            <  7:306707/135‑1 (MQ=255)
                                                                                                cgcAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCGGGGCGGGCTGGGCGGTTTGCAGGGCCGGTTGGCCGCGGTTGTTTTAGAAAACTATAATCAGCAGCACCCGGCTATGGCGGATTTGCTTGAACCaaa                           >  4:194681/1‑135 (MQ=255)
                                                                                                 gaaaaTCCTGCCCGCGGCGCGGTACGGCCCGGGGGGCCGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCaa                            <  2:308181/132‑1 (MQ=255)
                                                                                                 gaaaaTACGGCCCGCCGCGCTCGACGGCCGGGCGCGCGGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCCGCAGCACCAGGATATGGCGGATTTCCTTGACCCaa                            <  6:203178/132‑1 (MQ=255)
                                                                                                     aTACTCCCCGCCGCGCTCTAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCtt                       <  1:21181/135‑1 (MQ=255)
                                                                                                         tGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCGTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGccg                  >  4:33782/1‑135 (MQ=255)
                                                                                                            ccgccgcGCTCGTCGGCCTGGCGCGCCGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGa               <  3:121838/135‑1 (MQ=255)
                                                                                                              gccgcGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCg                >  7:42948/1‑133 (MQ=255)
                                                                                                              gccgcGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCGTTTTGCCGGGCCGGGTGGCCGAGGTTGTTGAAGAACTCTATATGCCTCAGCCCCCGGATATGGCCGCGTTCAGTTACCCAAATGTTGGCCTCGaaa             >  4:31427/1‑134 (MQ=255)
                                                                                                                   gcTCGAAGGCCACGCGCGCAAGGCCGGGCTGGCCTTTTTTCAGGGCCGGGTGGCCGAGGTTGTTTAAGAACTATATATTCATCAGCCCCACGATATGGCGGCTTTCCTTGAACCAATTCTTGGCCTCGACACCgg         >  6:16194/1‑135 (MQ=255)
                                                                                                                        acggCCCGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaag    <  7:104881/133‑1 (MQ=255)
                                                                                                                          ggCCCGGCGCGCCGGGCCGGGCTGGCCTTTTTGCAGGGCCCGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaag    <  5:67424/133‑1 (MQ=255)
                                                                                                                          ggCCAGGCGCGCCTGGCCGGGCTGGCCTTTATGCAGGGCCTGTTGGCCGAGGGTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaagaa  <  6:60847/135‑1 (MQ=255)
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GGGGCCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCAAGAA  >  ACB143/2094682‑2094938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑