Predicted mutation
evidence seq id position mutation annotation gene description
MC JC ACB143 5,390,191 Δ10 bp coding (285‑294/1666 nt) ligH → 10‑formyltetrahydrofolate synthetase

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ ACB143 5390191 5390200 10 45 [0] [0] 45 ligH 10‑formyltetrahydrofolate synthetase

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? ACB143 = 53901900 (0.000)45 (0.800) 32/256 0.4 100% coding (284/1666 nt) ligH 10‑formyltetrahydrofolate synthetase
?ACB143 5390201 = 0 (0.000)coding (295/1666 nt) ligH 10‑formyltetrahydrofolate synthetase

AGGGCAACCAGGACGGCAAGCTGATCCTCGTCACCGCGATCAACCCGACGCCGGCCGGCGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  ACB143/5390058‑5390190
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTC  >  ACB143/5390201‑5390316
                                                                                                                                                                                                                                                         
AGGGCAACCAGGACGGCAAGCTGATCCTCGTCACCGCGATCAACCCGACGCCGGCCGGCGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTTCGC                                                                                                                  >  4:57723/1‑137
    CAACCAGGACGGCAAGCTGATCCTCGTCACCGCGATCAACCCGACGCCGGCCGGCGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTTCGCTCGG                                                                                                              <  2:235310/137‑1
    CAACCAGGACGGCAAGCTGATCCTCGTCACCGCGATCAACCCGACGCCGGCCGGCGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTTCGCTCGG                                                                                                              <  4:106630/137‑1
               GCAAGCTGATCCTCGTCACCGCGATCAACCCGACGCCGGCCGGCGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTTCGCTCGGCCCCTGTTTCG                                                                                                   >  4:343411/1‑137
                 AAGCTGATCCTCGTCACCGCGATCAACCCGCCGCCGGCCGGCGAAGGCAAGAACACGAACACCTTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTTAGCTCGGCCCCTGTTTCGGC                                                                                                 <  3:51221/137‑1
                           TCGTCACCGCGATCAACCCGACGCCGGCCGGCGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTTCGCGCGGCCCCTGTTTCGGCGTGAAGGGCG                                                                                       >  2:232977/1‑137
                               CACCGCGATCAACCCGACGCCGGCCGGCGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGC                                                                                   <  5:347420/137‑1
                                ACCGCGATCAACCCGACGCCGGCCGGCGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCG                                                                                  >  5:126259/1‑137
                                 CCGCGATCAACCCGACGCCGGCCGGCGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCCGCGCGG                                                                                 >  5:269641/1‑137
                                 CCGCGATCAACCCGACGCCGGCCGGCGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAACACCGTGGCGGCGCTTCGCTCGGCCCCTGATTCGGCGTGAAGGGCGGAGCGc                                                                                 >  6:86170/1‑136
                                     GATCAACCCGACGCCGGCCGGCGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGG                                                                             >  7:179347/1‑137
                                         AACCCGACGCCGGCCGGCGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGC                                                                         <  8:89283/137‑1
                                          ACCCGACGCCGGCCGGCGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTTCGCTCGGCCGCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGccc                                                                        >  5:258319/1‑134
                                                    GGCCGGCGAAGGCAAGCCCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGACGCCCGTGGCGGCGCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCA                                                              <  1:50547/137‑1
                                                    GGCCGGCGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCA                                                              <  3:57723/137‑1
                                                    GGCCGGCGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGGGGCGGCGCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGTGCGGCCGGCGGCGGCTATGCGCA                                                              <  5:8834/137‑1
                                                        GGCGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTC                                                          >  7:266953/1‑137
                                                        GGCGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTC                                                          >  7:128041/1‑137
                                                        GGCGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTC                                                          >  4:30904/1‑137
                                                        GGCGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTC                                                          >  4:263417/1‑137
                                                         GCGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCG                                                         <  4:337168/137‑1
                                                               GCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGA                                                   <  6:320600/137‑1
                                                                 AAGACCACGACCACCGTGAGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATG                                                 <  3:248675/137‑1
                                                                     CCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTTCGCTCGGCCCCTGTGTCGGCGTGAAGGCCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGG                                             <  1:94193/137‑1
                                                                      CACGACCCCCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGA                                            <  5:287416/137‑1
                                                                      CACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGA                                            <  5:244037/137‑1
                                                                      CACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGA                                            <  1:87744/137‑1
                                                                        CGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACA                                          <  8:232260/137‑1
                                                                          ACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATC                                        <  3:263417/137‑1
                                                                           CCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCA                                       <  8:128041/137‑1
                                                                               CGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCT                                   <  2:318754/137‑1
                                                                                 TGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTC                                 >  5:131609/1‑137
                                                                                  GGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCA                                >  5:275924/1‑137
                                                                                    GCCTGGCCGACGGCCTCATCCGCATCGGCAAGAAGACCGTGGCGGCGCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCctgtct                              <  5:319364/137‑7
                                                                                         GCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACC                         <  1:166320/137‑1
                                                                                         GCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACC                         <  4:319135/137‑1
                                                                                            GACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGGGGACATCAACCTTCACGTCACCGGg                      >  7:50083/1‑136
                                                                                            GACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTTCGCTCGGCCCCGGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCGATGCGCAGGGCGTGCCGATGGCGGACATCCACCTTCCCTTCACCGGT                      >  8:326120/1‑137
                                                                                                GCCGCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACT                  <  1:158389/137‑1
                                                                                                         GCATCGGCAAGAAGACCGTGGCGGCGCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCA         <  8:133799/137‑1
                                                                                                            TCGGCAAGAAGACCGTGGCGGCGCTTCGCGCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGGCGATGGAGGACATCAACCTACACTTCACCGGTGACTTCCATGACATCA      >  2:321185/1‑137
                                                                                                             CGGCAAGAAGACCGTGGCGGCGCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCAC     <  3:30904/137‑1
                                                                                                             CGGCAAGAAGACCGTGGCGGCGCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCAC     <  8:266953/137‑1
                                                                                                               GCAAGAAGACCGTGGCGGCGCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCT   <  2:204235/137‑1
                                                                                                                CAAGAAGACCGTGGCGGCGCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTC  >  5:14588/1‑137
                                                                                                                                                                                                                                                         
AGGGCAACCAGGACGGCAAGCTGATCCTCGTCACCGCGATCAACCCGACGCCGGCCGGCGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  ACB143/5390058‑5390190
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTC  >  ACB143/5390201‑5390316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.