Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB143 5,386,519 G→A intergenic (‑528/+1123) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,386,5190GA100.0% 30.1 / NA 11intergenic (‑528/+1123)CJ019_05070/nptIIhypothetical protein/neomycin phosphotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/4);  total (7/4)

AAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGTCGT‑‑‑CAGG‑‑ATC‑‑ATCCGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGG  >  ACB143/5386427‑5386605
                                                                                                     |                                                                                      
aaaaaTTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGG‑‑TACAAACCCCGACGCTAGCATCGCATGAGAAAGCCCCCGGAAGATCACCTTc                                                >  2:277347/1‑137 (MQ=255)
          cGAATTCTTTCATTTAAGACCCTAATATGACATCTCGCCCGGCTTCTCGACGACGGCGGTCTCAGAGGTGTACGGT‑‑ACA‑‑AACCACGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCttt                                      >  3:111579/1‑137 (MQ=255)
              ttCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGG‑‑TACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTa                                  <  7:60037/137‑1 (MQ=255)
                           caCCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGG‑‑TACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGt                     <  6:12099/136‑1 (MQ=255)
                                 aaTATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGGTAAAA‑‑ACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCatg               <  5:293054/137‑3 (MQ=255)
                                 aaTATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGG‑‑TACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg               <  3:94555/137‑1 (MQ=255)
                                    atGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGG‑‑TACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGTGCTTTTTTATTGCGCTGCGTGTGCAAGGGCg            >  4:192268/1‑137 (MQ=255)
                                    atGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGG‑‑TACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCg            >  2:66454/1‑137 (MQ=255)
                                           ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGG‑‑TACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt     >  8:236801/1‑137 (MQ=255)
                                           ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGG‑‑TACAAACCCAGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt     >  5:357066/1‑137 (MQ=255)
                                              ggccggcTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGG‑‑TACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTggg  >  1:121920/1‑137 (MQ=255)
                                                                                                     |                                                                                      
AAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGTCGT‑‑‑CAGG‑‑ATC‑‑ATCCGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGG  >  ACB143/5386427‑5386605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: