Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 838,759 G→T 12.9% I94I (ATC→ATA)  CJ019_00802 ← L‑glutamine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB143838,7590GT12.9% 81.2 / 4.7 31I94I (ATC→ATA) CJ019_00802L‑glutamine kinase
Reads supporting (aligned to +/- strand):  ref base G (15/12);  new base T (2/2);  total (17/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.99e-01

CCAGTCAAAATAACTCTCCGGAGTCTCGTCGTAGCGCGCCACACGTATATCGTAGGTACCTGGCCGGACATGACCGTGGAGCTTTAGGAAATCGTCCTTATGCAACGTCTGGAAATCGCTGGCCATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTT  >  ACB143/838624‑838893
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ccAGTCAAAATAACTCTCCGGAGTCTCGTCGTAGCGCGCCACACGTATATCGTAGGTACCTGGCCGGACATGACCGTGGAGCTTTAGGAAATCGTCCTTATGCAACGTCTGGAAATCGCTGGCCATCTCGCGACCg                                                                                                                                        <  2:79319/136‑1 (MQ=255)
 cAGTCAAAATAACTCTCCGGAGTCTCGTCGTAGCGCGCCACACGTATATCGTAGGTACCTGGCCGGACATGACCGTGGAGCTTTAGGAAATCGTCCTTATGCAACGTCTGGAAATCGCTGGCCATCTCGCGACCGa                                                                                                                                       >  6:227203/1‑136 (MQ=255)
 cAGTCAAAATAACTCTCCGGAGTCTCGTCGTAGCGCGCCACACGTATATCGTAGGTACCTGGCCGGACATGACCGTGGAGCTTTAGGAAATCGTCCTTATGCAACGTCTGGAAATCGCTGGCCATCTCGCGACCGa                                                                                                                                       >  4:209961/1‑136 (MQ=255)
                  cGGAGTCTCGTCGTAGCGCGCCACACGTATATCGTAGGTACCTGGCCGGACATGACCGTGGAGCTTTAGGAAATCGTCCTTATGCAACGTCTGGAAATCGCTGGCCATCTCGCGACCGATACTATTGACCCCTcc                                                                                                                       <  7:110317/135‑1 (MQ=255)
                   ggAGTCTCGTCGTAGCGCGCCACACGTATATCGTAGGTACCTGGCCGGACATGACCGTGGAGCTTTAGGAAATCGTCCTTATGCAACGTCTGGAAATCGCTGGCCATCTCGCGACCGATACTATTGACCCCTCCgg                                                                                                                     <  6:37005/136‑1 (MQ=255)
                             cgtAGCGCGCCACACGTATATCGTAGGTACCTGGCCGGACATGACCGTGGAGCTTTAGGAAATCGTCCTTATGCAACGTCTGGAAATCGCTGGCCATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATc                                                                                                           >  5:171583/1‑136 (MQ=255)
                                     gcCACACGTATATCGTAGGTACCTGGCCGGACATGACCGTGGAGCTTTAGGAAATCGTCCTTATGCAACGTCTGGAAATCGCTGGCCATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAAc                                                                                                   <  1:72389/136‑1 (MQ=255)
                                       cacaCGTATATCGTAGGTACCTGGCCGGACATGACCGTGGAGCTTTAGGAAATCGTCCTTATGCAACGTCTGGAAATCGCTGGCCATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCg                                                                                                 >  7:138603/1‑136 (MQ=255)
                                          acGTATATCGGATGTACCTGGCCGGACATGACCGTGTAGATTTAGGAAATCTTCCTTATGCAACGTCTGGAAATCGCTGGCGATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCa                                                                                              <  8:266726/136‑1 (MQ=255)
                                            gTATATCGTAGGTACCTGGCCGGACATGACCGTGGAGCTTTAGGAAATCGTCCTTATGCAACGTCTGGAAATCGCTGGCCATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGa                                                                                            <  7:226607/136‑1 (MQ=255)
                                                       gTACCTGGCCGGACATGACCGTGGAGCTTTAGGAAATCGTCCTTATGCAACGTCTGGAAATCGCTGGCCATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCg                                                                                 >  1:61538/1‑136 (MQ=255)
                                                         aCCTGGCCGGACATGACCGTGGAGCTTTAGGAAATCGTCCTTATGCAACGTCTGGAAATCGCTGGCCATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCt                                                                               >  7:54865/1‑136 (MQ=255)
                                                         aCCTGGCCGGACATGACCGTGGAGCTTTAGGAAATCGTCCTTATGCAACGTCTGGAAATCGCTGGCCATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCt                                                                               >  5:55592/1‑136 (MQ=255)
                                                         aCCTGGCCGGACATGACCGTGGAGCTTTAGGAAATCGTCCTTATGCAACGTCTGGAAATCGCTGGCCATCTCGCGACAGATACTATTGACCACTCCGGTCAAATGATAGATGAAACCGGCATCTACTACCACCgag                                                                               >  6:188177/1‑134 (MQ=255)
                                                           cTGGCCGGACATGACCGTGGAGCTTTAGGAAATCGTCCTTATGCAACGTCTGGAAATCGCTGGCCATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTGt                                                                             >  2:93867/1‑136 (MQ=255)
                                                              gCCGGACATGACCGTGGAGCTTTAGGAAATCGTCCTTATGCAACGTCTGGAAATCGCTGGCCATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTGttt                                                                           <  5:105807/135‑1 (MQ=255)
                                                               ccGGACATGACCGTGGAGCTTTAGGAAATCGTCCTTATGCAACGTCTGGAAATCGCTGGCCATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTcc                                                                         <  5:148171/136‑1 (MQ=255)
                                                                     aTGACCGTGGAGCTTTAGGAAATCGTCCTTATGCAACGTCTGGAAATCGCTGGCCATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACTGGCAGATACTACCCCCGCTGTTTCCAGCGAt                                                                   >  5:11501/1‑136 (MQ=255)
                                                                               aGCTTTAGGAAATCGTCCTTATGCAACGTCTGGAAATCGCTGGCCATCTCGCGACCTATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTAccc                                                                                    >  7:113719/1‑109 (MQ=255)
                                                                               aGCTTTAGGAAATCGTCCTTATGCAACGTCTGGAAATCGCTGGCCATCTCGCGACCTATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTAccc                                                                                    >  7:117728/1‑109 (MQ=255)
                                                                               aGCTTTAGGAAATCGTCCTTATGCAACGTCTGGAAATCGCTGGCCATCTCGCGACCTATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTAccc                                                                                    <  8:113719/109‑1 (MQ=255)
                                                                               aGCTTTAGGAAATCGTCCTTATGCAACGTCTGGAAATCGCTGGCCATCTCGCGACCTATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTAccc                                                                                    <  8:117728/109‑1 (MQ=255)
                                                                               aGCTTTAGGAAATCGTCCTTATGCAACGTCTGGAAATCGCTGGCCATCTCGCGACCGATACTATTGACCCCTCCGCTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTCTTTCCAGCGATTTAATTATTg                                                         >  3:89795/1‑136 (MQ=255)
                                                                                gCTTAAGGAAATCGTACTTATCCATCGTATGGAAATCGCTGGCCATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATTAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTg                                                         <  8:205868/135‑1 (MQ=255)
                                                                                 cTTTAGGAAATCGTCCTTATGCAACGTCTGGAAATCGCTGGCCATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCg                                                       <  3:254472/136‑1 (MQ=255)
                                                                                         aaTCGTCCTTATGCAACGTCTGGAAATCGCTGGCCATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTAc                                               <  1:93867/136‑1 (MQ=255)
                                                                                          atggTCCTTATGCAACGTCTGGAAATCGCTGGCCAGCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACg                                              <  6:198414/133‑1 (MQ=255)
                                                                                              tCCTTATGCAACGTCTGGAAATCGCTGGCCATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACg                                          >  1:81511/1‑136 (MQ=255)
                                                                                              tCCTTATGCAACGTCTGGAAATCGCTGGCCATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCCGTACCTACGAACg                                          >  7:83320/1‑136 (MQ=255)
                                                                                                   aTGCAACGTCTGGAAATCGCTGGCCATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCa                                     >  7:8133/1‑136 (MQ=255)
                                                                                                              ggAAATCGCTGGCCATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAgcgc                           >  6:235483/1‑135 (MQ=255)
                                                                                                                                      cGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGtt  <  4:172375/136‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
CCAGTCAAAATAACTCTCCGGAGTCTCGTCGTAGCGCGCCACACGTATATCGTAGGTACCTGGCCGGACATGACCGTGGAGCTTTAGGAAATCGTCCTTATGCAACGTCTGGAAATCGCTGGCCATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTT  >  ACB143/838624‑838893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑