Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 4,807,149 G→C 12.0% S66T (AGC→ACC)  mcbC → Microcin B17‑processing protein McbC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,807,1490GC12.0% 414.0 / 6.2 144S66T (AGC→ACC) mcbCMicrocin B17‑processing protein McbC
Reads supporting (aligned to +/- strand):  ref base G (85/38);  new base C (2/17);  total (87/57)
Fisher's exact test for biased strand distribution p-value = 1.47e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 145 total aligned reads displayed.

GGCTTTCCATGCCAAAGGCAACTATACGATACACAAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGCTCTTAACT  >  ACB143/4807015‑4807282
                                                                                                                                      |                                                                                                                                     
gtcTTTCCATGCCAAAGGCAACTATACGATACACAAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAg                                                                                                                                       <  6:204477/133‑1 (MQ=255)
  cTTTCCATGCCAAAGGCAACTATACGATACACAAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAACACTCAATACCATTGTCGCCGCAAGCa                                                                                                                                     >  6:76376/1‑135 (MQ=255)
  cTTTCCATGCCAAAGGCAACTATACGATACACAAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCa                                                                                                                                     >  7:106978/1‑135 (MQ=255)
  cTTTCCATGCCAAAGGCAACTATACGATACACAAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCa                                                                                                                                     >  1:250866/1‑135 (MQ=255)
  cTTTCCATGCCAAAGGCAACTATACGATACACAAAGCAACTCAACACTTAAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCa                                                                                                                                     >  8:122765/1‑135 (MQ=255)
     tCCATGAAAAAGTAAAATATACTATACAAAAAGCAACTAAACAGTTGAGCGCCCTGCACAAAATACCACCAAAAGACCTCGAAAAACTTACCGTAAACGAACTAAAACTCAATACATTTTTAGCCCAAAGAaaaa                                                                                                                                  <  7:4256/135‑1 (MQ=255)
                 gCAACTATACGATACACAAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGAc                                                                                                                     >  5:189534/1‑136 (MQ=255)
                   aaCTATACGATACACAAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAACAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGActc                                                                                                                   <  2:221778/136‑1 (MQ=255)
                             aacacAAACCAACTCAACACTTGAGCGCCCTGCACCAAAAACCACAAAAAGACCACCAAAAACTTACCGCAAACTAACTAAAACTCAATACCATTTTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACctct                                                                                                         <  4:265226/135‑1 (MQ=255)
                                acaAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGc                                                                                                      >  8:82045/1‑136 (MQ=255)
                                acaAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGc                                                                                                      >  7:216929/1‑136 (MQ=255)
                                acaAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGc                                                                                                      >  6:133817/1‑136 (MQ=255)
                                acaAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGc                                                                                                      >  5:258877/1‑136 (MQ=255)
                                acaAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGc                                                                                                      >  5:124363/1‑136 (MQ=255)
                                acaAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTAAACCTCTGGc                                                                                                      >  1:18517/1‑136 (MQ=255)
                                acaAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACAGACTCTTACACCTCTGGc                                                                                                      >  5:292541/1‑136 (MQ=255)
                                  aaaGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGc                                                                                                      >  6:159721/1‑134 (MQ=255)
                                  aaaGCAACTCAACACTTGAGCGCCCTGCACAAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAACCAAAAACAAAGAACCGACTCTTACACCTCTgtc                                                                                                      >  8:60469/1‑132 (MQ=255)
                                   aaGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAg                                                                                                   >  1:100834/1‑136 (MQ=255)
                                   aaGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAg                                                                                                   >  2:43613/1‑136 (MQ=255)
                                   aaGCAACTCAACACTTGAGCGCCCTGCACCAAATACAACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAg                                                                                                   >  8:159834/1‑136 (MQ=255)
                                     gCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGCCTCTTACACCTCTGGCGAGcc                                                                                                 >  8:240677/1‑136 (MQ=255)
                                     gCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGcc                                                                                                 >  8:80608/1‑136 (MQ=255)
                                     gCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGcc                                                                                                 >  1:178767/1‑136 (MQ=255)
                                       aaCTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTAACGGAAACGAACTAAAACTCAATACCATTGTAGCCCCACGCAAAAACCAAGAACCGACTCTTACACCTCTGGCGAGACca                                                                                               >  8:267719/1‑136 (MQ=255)
                                        aCTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAACACCTCGAAAAACTTCCCGTAAACCAACTAAAACTCAATCCCATTGTACCCCCAACCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCcac                                                                                              <  4:172815/136‑1 (MQ=255)
                                          tCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCacac                                                                                            >  4:109676/1‑136 (MQ=255)
                                           cAACACTTGAGCGCCCTCCACAAAAAACCACCAACAGACCTCAAAAAAATTACCGAAACCCAAAAAAAACACAAACCCATTCTACCCCCACCCAAAAAAAAACACCCGACTCTTACACCTCTGGCGAGCCCACAcc                                                                                           <  2:142166/136‑1 (MQ=255)
                                            aaCACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAACAACCGACTCTTACACCACTCGCGAGCCCACAccc                                                                                          >  8:32834/1‑136 (MQ=255)
                                            aaCACTTGAGCGCCCTCCACCAAATACCACCAAAAGACCTCGAAAATCTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGAAGCTTACACCTCTGGCGAGCCCACAccc                                                                                          >  8:214035/1‑136 (MQ=255)
                                               acTTTAGCGCCCTGCACAAAATACCACCAAAACACCTCAAAAAACTTACCTTAAACCAACAAAAACTAAATACCATTGTACCCCCAACCAAAAACAAACAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCt                                                                                       <  1:135928/136‑1 (MQ=255)
                                                 ttGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACAGACTCTTACACCTCTGGCGAGCCCACACCCGCTAc                                                                                     >  2:45058/1‑136 (MQ=255)
                                                       gcgcTGCACCAAATACCACCAAAAGACCACGAAAAACTTACCGGAAACGAACTAAAACTCAAAACCATTGTAGACCCAAGCAAAAACTAAGAACCGACTCTTACACATCTGGCGAGCCCACACCCGCAACgaaga                                                                                >  2:3673/4‑135 (MQ=255)
                                                       gcCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACgaaga                                                                                >  3:33959/1‑135 (MQ=255)
                                                       gcCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAAAAACATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACgaaga                                                                                >  3:75357/1‑135 (MQ=255)
                                                            gCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGt                                                                          >  3:187967/1‑136 (MQ=255)
                                                              aCCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGCGCCCACACCCGCTACGAAGAAATAGtt                                                                         >  2:101161/1‑135 (MQ=255)
                                                              aCCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGtt                                                                         >  6:223521/1‑135 (MQ=255)
                                                                  aaTACCACCAAACCACCTCGAAAAAATTACCCTAAACGACATAAAACTCAATACCATTTTAGCCCCAACAAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGc                                                                    <  8:151012/136‑1 (MQ=255)
                                                                  aaTACCACCAAAAGACCTCGAAAAACTTCCCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCACGCAAAAACAAACAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGc                                                                    <  1:93032/136‑1 (MQ=255)
                                                                  aaTACCACCAAAACACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGAAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGc                                                                    <  8:69113/136‑1 (MQ=255)
                                                                     accaccAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACCCCCGCTACGAAGAAATAGTGCATGCGaa                                                                 >  4:2815/1‑136 (MQ=255)
                                                                     accaccAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGaa                                                                 >  1:136783/1‑136 (MQ=255)
                                                                          caAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGAtt                                                            >  3:111066/1‑136 (MQ=255)
                                                                          caAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGAtt                                                            >  3:140959/1‑136 (MQ=255)
                                                                          caAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGAtt                                                            >  1:65524/1‑136 (MQ=255)
                                                                          caAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGAtt                                                            >  7:172021/1‑136 (MQ=255)
                                                                          caAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGAtt                                                            >  7:93913/1‑136 (MQ=255)
                                                                          caAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCACAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGAtt                                                            >  7:11561/1‑136 (MQ=255)
                                                                          caAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAAAACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGAtt                                                            >  4:247206/1‑136 (MQ=255)
                                                                          caAAACACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAACCAAAAACAAATAACCCACTATTACACATCAGGAGAGCCCACAACCGCTACGAAGACATAGTTCATCCGAAAGAt                                                             >  4:11744/1‑135 (MQ=255)
                                                                               gACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAg                                                       >  4:76189/1‑136 (MQ=255)
                                                                               gACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAg                                                       >  5:191308/1‑136 (MQ=255)
                                                                               gACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACAACCGCTACGAAGAAATAGATCATGCGAACGAATTCAAg                                                       >  4:227737/1‑136 (MQ=255)
                                                                                  atctaaaaaCATACTGGAAAGCAAGAAAAACTCAATACATTTGTCGCCCAAATCAAATACAATTTACCGACTCTTACACCTCTGTCGAGCCCACACCCGCTACTAAGAAATAGTTCATGCTAACGATTTGAAGCAc                                                    <  4:194087/132‑1 (MQ=255)
                                                                                    cGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGc                                                  >  6:238038/1‑136 (MQ=255)
                                                                                    cGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGc                                                  >  6:18138/1‑136 (MQ=255)
                                                                                       aaaaCTTCCCGCAAACCAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAACAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCct                                               <  1:257257/136‑1 (MQ=255)
                                                                                       aaaaCTTACCGGACACCAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCct                                               <  2:35469/136‑1 (MQ=255)
                                                                                       aaaaCTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGCGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCct                                               >  6:87042/1‑136 (MQ=255)
                                                                                       aaaaCTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTACATGCGAACGATTTGAAGCACGCCct                                               >  5:269480/1‑136 (MQ=255)
                                                                                       aaaaCTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAACAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCct                                               <  3:16966/136‑1 (MQ=255)
                                                                                       aaaaCTTACCGCAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCct                                               <  1:38233/136‑1 (MQ=255)
                                                                                       aaaaATTACTGCAAACTAAATAAAACTCAATACCATTGAAGCCCAAACAAAAAAAAAACAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCct                                               <  4:161080/136‑1 (MQ=255)
                                                                                           ctaaCCGCAAACGAACAAAAACTCAATACCATTCAACCCCCAACCAAAAACAACCAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTc                                           <  3:141934/133‑1 (MQ=255)
                                                                                           cTTACCGTAAACTAACTAAAACTCAATACCATTGTAGCCCCAACCAAAAACAAACAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTc                                           <  3:279488/136‑1 (MQ=255)
                                                                                                cGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACtat                                      >  2:84891/1‑134 (MQ=255)
                                                                                                 ggAAACGAACTAAAACTCAATACCTTTGTAGCCCCAAGAAAAAACAAACAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTg                                     <  7:32834/136‑1 (MQ=255)
                                                                                                 ggAAACGAACTAAAACTCAATACCATTGTCGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTg                                     <  4:87632/136‑1 (MQ=255)
                                                                                                 ggAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTg                                     <  2:246956/136‑1 (MQ=255)
                                                                                                 ggAAACGAACTAAAACTCAATACCATTGTAGCCCCAACCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTg                                     <  5:55037/136‑1 (MQ=255)
                                                                                                 gcaacagAACTAAATCAAAATACCATTGTTGCCCATATCTATAAATAAGAACCGACTCTTACACCTCTGGAGATCCCAGACACGCTACGAAGAAATTGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTg                                     <  2:272411/130‑1 (MQ=255)
                                                                                                 gcaaaCTAACTAAAACTCAATACCATTGTAGCCCCAAGAAAAAACAAACAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTg                                     <  5:172474/134‑1 (MQ=255)
                                                                                                           tAAAACTCAATACCATTGTAGCCCAAAGAAAAAACAAAGACCCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCa                           <  4:81007/136‑1 (MQ=255)
                                                                                                           aaaaaCTCAATACAATTTTAGCCCCAAGAAAAAAAAAACAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCa                           <  2:139031/135‑1 (MQ=255)
                                                                                                             aaaCTCAATACCATTGTAGCCCCAAGAAAAAACAAACACCCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCa                           <  6:106662/134‑1 (MQ=255)
                                                                                                                     tACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCAttgctt                 <  5:97395/136‑1 (MQ=255)
                                                                                                                      aCCATTGTAGCCCCACGCAAAAACAAACAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCAttgcttg                <  1:101273/136‑1 (MQ=255)
                                                                                                                      aCCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCAttgcttg                >  3:94497/1‑136 (MQ=255)
                                                                                                                      aCCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCAttgcttg                >  8:102668/1‑136 (MQ=255)
                                                                                                                             tAGCCCCAAGCAAAAACAAAGACCCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGct         >  3:164302/1‑136 (MQ=255)
                                                                                                                             tAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGct         >  4:194519/1‑136 (MQ=255)
                                                                                                                             tAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGct         >  3:193278/1‑136 (MQ=255)
                                                                                                                             tAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGct         >  7:221674/1‑136 (MQ=255)
                                                                                                                             tAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGct         >  4:20800/1‑136 (MQ=255)
                                                                                                                             tAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGct         >  6:178382/1‑136 (MQ=255)
                                                                                                                             tAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGATCATGCGAACGATTTGAATCACGCCCTCTTCACTTAGATATAGTCCAAGCATTGCTTGCACCGct         >  1:146830/1‑136 (MQ=255)
                                                                                                                             tAGCCCAAAGAAAAACCCAATCACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGTAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGc          <  1:101994/135‑1 (MQ=255)
                                                                                                                              aGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGctc        >  5:166325/1‑136 (MQ=255)
                                                                                                                                  ccAAGCAAAAACAAAGAACCGACTCTTACACctct                                                                                                         <  6:52266/35‑1 (MQ=255)
                                                                                                                                  ccAAGCAAAAACAAAGAACCGACTCTTACACctct                                                                                                         >  5:52266/1‑35 (MQ=255)
                                                                                                                                  ccAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGCTCTTaa    >  7:196570/1‑136 (MQ=255)
                                                                                                                                  ccAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGCTCTTa     >  2:185986/1‑135 (MQ=255)
                                                                                                                                  ccAAGCAAAAACAAAGAACAGACTCTTACACCTCTGGCGAGCCCACACCAGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCAAAGCATTGCTTGCACCGCTCTTaa    >  2:201315/1‑136 (MQ=255)
                                                                                                                                   cAAGCAAAAACAAAGAACCGACTCTTACACCTCTgg                                                                                                       <  2:84428/36‑1 (MQ=255)
                                                                                                                                   cAAGCAAAAACAAAGAACCGACTCTTACACCTCTgg                                                                                                       >  1:84428/1‑36 (MQ=255)
                                                                                                                                    aaGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGCTCTTAACt  <  6:55112/136‑1 (MQ=255)
                                                                                                                                    aaGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGCTCTTAACt  <  1:158597/136‑1 (MQ=255)
                                                                                                                                     aGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTg                                     <  3:81488/100‑1 (MQ=255)
                                                                                                                                     aGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGCTCTTAACt  >  7:157191/1‑135 (MQ=255)
                                                                                                                                      |                                                                                                                                     
GGCTTTCCATGCCAAAGGCAACTATACGATACACAAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGCTCTTAACT  >  ACB143/4807015‑4807282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑