Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB143 120,983 (T)9→8 intergenic (+280/+92) polA → / ← engB DNA polymerase I/putative GTP‑binding protein EngB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB143120,9750T.96.3% 349.2 / 7.8 83intergenic (+272/+100)polA/engBDNA polymerase I/putative GTP‑binding protein EngB
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base . (52/26);  total (53/30)
Fisher's exact test for biased strand distribution p-value = 2.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACTGAATGGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGTTCCATC  >  ACB143/120849‑121105
                                                                                                                              |                                                                                                                                  
aaCTGAATGGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGACCTTCCCCCTCCCCATACGAAGTCCGGGGttttttttg                                                                                                                            >  4:116099/1‑134 (MQ=255)
    gAATGGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTATGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTAGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGttttttttgacc                                                                                                                         >  1:168472/1‑130 (MQ=255)
     aaTGGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCgg                                                                                                                      >  6:316675/1‑135 (MQ=255)
          cTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGACCTTCCCCCTCCCCATACGAAGTCCGGGGTGTTTTTTGCCCGGAAttt                                                                                                                 >  4:311419/1‑135 (MQ=255)
            ggTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGc                                                                                                               >  2:360226/1‑135 (MQ=255)
            ggTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGACCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGc                                                                                                               >  2:277906/1‑135 (MQ=255)
                      ttCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCggg                                                                                                     >  3:193018/1‑135 (MQ=255)
                                 tATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGa                                                                                          <  2:274040/135‑1 (MQ=255)
                                 tATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGa                                                                                          >  3:235359/1‑135 (MQ=255)
                                 tATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGa                                                                                          >  1:125994/1‑135 (MQ=255)
                                 tATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGa                                                                                          <  8:314375/135‑1 (MQ=255)
                                   tgttgtGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCGGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGATCGGGTTTTCTCGCGAAt                                                                                        <  2:371859/135‑1 (MQ=255)
                                   tgttgtGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt                                                                                        <  5:76929/135‑1 (MQ=255)
                                    gttgtGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt                                                                                        <  7:112690/134‑1 (MQ=255)
                                      tgtgTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCg                                                                                     >  4:131001/1‑135 (MQ=255)
                                        tgtTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCAAACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATg                                                                                  >  5:111558/1‑136 (MQ=255)
                                           tAAGTGTTGGCAGATATCTTGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTACGGGGTTTTATTTGCCAGGAATTTGCCGGGAGCCGGTTTACACCCGAATACgtgta                                                                                 >  2:253547/1‑130 (MQ=255)
                                           tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTTCCCGGAATTTGCCGGGAGCGGGTTTTCTAGCGAATTCGATGTc                                                                                >  5:294146/1‑135 (MQ=255)
                                           tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGt                                                                                 >  8:32818/1‑134 (MQ=255)
                                           tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGt                                                                                 >  3:144595/1‑134 (MQ=255)
                                           tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGt                                                                                 >  2:282720/1‑134 (MQ=255)
                                           tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGt                                                                                 >  4:276399/1‑134 (MQ=255)
                                           tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGt                                                                                 >  7:67603/1‑134 (MQ=255)
                                           tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGt                                                                                 >  7:38540/1‑134 (MQ=255)
                                           tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTc                                                                                >  5:373390/1‑135 (MQ=255)
                                           tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTc                                                                                >  2:117574/1‑135 (MQ=255)
                                           tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCAGGAATTTGCCGGGAGCGGGTTTTCTCTCGAATGCGATGt                                                                                 >  8:165090/1‑134 (MQ=255)
                                           tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGCTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTAGCGAATGCGATGt                                                                                 >  1:18743/1‑134 (MQ=255)
                                           tAAGTGTTGGCAGATATCCGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGt                                                                                 >  1:104782/1‑134 (MQ=255)
                                             aGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGaa                                                                             >  6:360798/1‑136 (MQ=255)
                                             aGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGa                                                                              >  3:191672/1‑135 (MQ=255)
                                                gtTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAAt                                                                            >  1:378627/1‑134 (MQ=255)
                                                     cAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACAccgc                                                                     >  5:389673/1‑136 (MQ=255)
                                                     cAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACAccg                                                                      >  3:312200/1‑135 (MQ=255)
                                                       gATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACAccgcc                                                                    >  4:366040/1‑135 (MQ=255)
                                                          atCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCAt                                                                >  5:19068/1‑136 (MQ=255)
                                                           tCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCAt                                                                <  6:391230/135‑1 (MQ=255)
                                                            cTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt                                                                                        <  8:374926/110‑1 (MQ=255)
                                                            cTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt                                                                                        >  7:374926/1‑110 (MQ=255)
                                                            cTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCa                                                                 >  1:64477/1‑133 (MQ=255)
                                                               gACCCCGCCCTAGCGGTCCGGACTTGGACCACGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGTTGTCGAATACACCGCCGCATTcgcg                                                           <  5:86416/136‑1 (MQ=255)
                                                                cccccGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTcgcg                                                           <  2:211511/134‑1 (MQ=255)
                                                                      cccTAGCGGTCCGGACTTGGCCCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTaaa                                                     <  7:353907/135‑1 (MQ=255)
                                                                       ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCg                                                                                     <  4:5437/102‑1 (MQ=255)
                                                                       ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCg                                                                                     >  3:5437/1‑102 (MQ=255)
                                                                       ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGATTACACCGCCGCAGTCGCGGGTAAAc                                                    >  8:251688/1‑135 (MQ=255)
                                                                       ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAAcc                                                   >  5:44724/1‑136 (MQ=255)
                                                                       ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAAcc                                                   >  5:363822/1‑136 (MQ=255)
                                                                       ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAAc                                                    >  7:86623/1‑135 (MQ=255)
                                                                       ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAAc                                                    >  1:19575/1‑135 (MQ=255)
                                                                       ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAAc                                                    >  3:254303/1‑135 (MQ=255)
                                                                       ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCCGGTAAAcc                                                   >  6:239755/1‑136 (MQ=255)
                                                                            cGGTCCGGACTTGCACCCCGAACTTCCCCCTCCCCATCCGAAGTCCGGGGTTTTTGTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCTATGTCTAATACACCGCCGCATTCGCGGGTAAACCCg                                                 <  4:331580/133‑1 (MQ=255)
                                                                                ccGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑‑TTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGgc                                         <  6:262164/136‑1 (MQ=255)
                                                                                  ggACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGgc                                         <  3:41375/135‑1 (MQ=255)
                                                                                  ggACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGgc                                         <  2:61540/135‑1 (MQ=255)
                                                                                  ggACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGgc                                         <  7:106934/135‑1 (MQ=255)
                                                                                  ggACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGgc                                         <  7:374469/135‑1 (MQ=255)
                                                                                    aCTTGGATCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGgcg                                        >  7:191897/1‑134 (MQ=255)
                                                                                    aCTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCa                                      <  5:316675/136‑1 (MQ=255)
                                                                                    aCTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCAGGAATTTCCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCCCATTCGCGGGTAAACACGGGTCAGgcg                                        >  7:204815/1‑134 (MQ=255)
                                                                                     cTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCa                                      <  8:86623/135‑1 (MQ=255)
                                                                                            cccGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCAc                                >  2:315751/1‑134 (MQ=255)
                                                                                            cccGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCAc                                >  8:149226/1‑134 (MQ=255)
                                                                                               gAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTaaa                            <  1:100360/135‑1 (MQ=255)
                                                                                                 aCTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATTTCGAATACACCGACGAATTCGCGGGTAAACCCGGGTCAGGCGCCAACCAATAAACg                          >  8:289592/1‑135 (MQ=255)
                                                                                                 aCTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGATTACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAACCCACTAAACg                          >  7:340567/1‑135 (MQ=255)
                                                                                                 aCTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACg                          >  3:187519/1‑135 (MQ=255)
                                                                                                 aCTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACg                          >  1:29112/1‑135 (MQ=255)
                                                                                                      ccccTCCCCATACGACGTCCGGGGTTTTTTTTGCCCGGATTTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCTAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGAcc                       <  7:253192/133‑1 (MQ=39)
                                                                                                       cccTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGc                    <  4:193018/135‑1 (MQ=255)
                                                                                                       cccTCCCCATACGAAGGCGGGGTTTTTTTTTGCCCGTATTTTGCGGGGTGCGGTTTTTCGCGCTAATGCGTTGTCGAAAACACCGCCGCATTCGGGGGTAAACCCGGGGCAGGCGCAAACCACTACACGACCGGc                    <  4:187519/135‑1 (MQ=255)
                                                                                                       cccTCCCAATACGAAGTCCGGGGTTTTTTTTGCCCGTAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCTATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGc                    <  1:114913/135‑1 (MQ=39)
                                                                                                        ccTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCt                   >  4:64939/1‑135 (MQ=255)
                                                                                                           ccccATACGACGTCGGGGGTTTTTTTTGCCCGTATTTTGCCGGTTGGGGGTTTTCTCGCTAATGCGTTGTCTAAAACACCGCCGCATTCGCGGGTAAACCGGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTc               <  6:44724/136‑1 (MQ=255)
                                                                                                           ccccATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGt                >  2:280255/1‑135 (MQ=255)
                                                                                                           cccaATACGACGTCCTGGTTTTTTTTTGCCCGGAATTTGCCGGGAGGGGGTTTTATCCTTAATGCGATGTCCAAAACACCCCCCCATTCGGGGTTAAACCCGGGGCTGGCTCAAACAAATACACGACCGGCTTGTc               <  6:224374/136‑1 (MQ=255)
                                                                                                            cccATCCTAAGTCCGGGGTTTTTTTTGCCCTTAATTTGCCGGGAGCGGGTTTTCTCGATATTGCGATGTCTAATACACCGCCGCATTCGCGGGTAAACCCGTGTCAGGCGCAAACCACTAAACGACCGGCTTGTc               <  2:108171/135‑1 (MQ=255)
                                                                                                            cccATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCTAATACACCGCCGCATTCGCGGTTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTc               <  4:144595/135‑1 (MQ=255)
                                                                                                            ccaaTACGAAGTCGGGGGTTTTTTTTGCCCGGAATTTGCCGGTAGCGGGTTATCTCGCTAATGCGTTGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTc               <  3:92541/132‑1 (MQ=255)
                                                                                                               aTACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGttt                                                                                                  <  1:210240/49‑1 (MQ=255)
                                                                                                               aTACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGttt                                                                                                  >  2:210240/1‑49 (MQ=255)
                                                                                                                  cGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGAGGGAAAACCCGGGTAAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAg         >  7:305015/1‑135 (MQ=255)
                                                                                                                   gAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGt        >  1:330795/1‑135 (MQ=255)
                                                                                                                   gAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGt        >  2:317837/1‑135 (MQ=255)
                                                                                                                         ccggggTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGTtccatc  >  5:391380/3‑136 (MQ=255)
                                                                                                                         ccggggTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGTtccatc  >  6:292710/3‑136 (MQ=255)
                                                                                                                         ccggggTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGTtccat   >  7:383729/3‑135 (MQ=255)
                                                                                                                              |                                                                                                                                  
AACTGAATGGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGTTCCATC  >  ACB143/120849‑121105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: