Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	ACB143	4,257,335	+GCGGCCGC	intergenic (‑281/+7)	CJ019_03991 ← / ← CJ019_03994	putative ABC transporter ATP‑binding protein/hypothetical protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	ACB143	= 4257334	0 (0.000)	51 (0.820) +CGCGGCCG	30/242	0.7	100%	intergenic (‑280/+8)	CJ019_03991/CJ019_03994	putative ABC transporter ATP‑binding protein/hypothetical protein
	?	ACB143	4257335 =	0 (0.000)					intergenic (‑281/+7)	CJ019_03991/CJ019_03994	putative ABC transporter ATP‑binding protein/hypothetical protein

GCCCCATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  ACB143/4257202‑4257334 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCATGATCGCTTCGGCGCGGGCACCCTG  >  ACB143/4257335‑4257461                                                                                                                                      ||||||||                                                                                                                                GCCCCATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGC                                                                                                                                      >  6:266581/1‑136     CATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCC                                                                                                                                  <  6:330612/136‑1      ATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCC                                                                                                                                  <  8:19103/135‑1      ATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCC                                                                                                                                  >  2:193461/1‑135      ATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAAGAAAAACAACACCCGGAGAGCCTCCCGCGGCC                                                                                                                                  >  8:88110/1‑135       TCATTGGATCCACTAAAAACGGATAATGGCACTGAATCGGCACCTACCGAACAAAATGCATTGACCTACCACGCGTACCGGAGCCTTAATGTTTCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCG                                                                                                                                 <  2:10821/135‑1       TCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCG                                                                                                                                 <  3:179003/135‑1                  ACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTAC                                                                                                                      >  5:141743/1‑135                  ACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTAC                                                                                                                      >  5:132900/1‑135                  ACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTAC                                                                                                                      <  2:286554/135‑1                    TAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGA                                                                                                                    >  4:391592/1‑135                    TAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGA                                                                                                                    >  4:267820/1‑135                      AAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCAGCGGCCGCCCCGTCTTACGA                                                                                                                    >  2:137489/1‑133                       AAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATTCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGA                                                                                                                 <  2:227710/135‑1                         ACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGG                                                                                                               <  3:178363/135‑1                            GATAATCGCACACAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAG                                                                                                           <  6:25323/136‑1                                       AGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTC                                                                                                 >  4:276703/1‑135                                        GAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCA                                                                                                >  4:323381/1‑135                                               CACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTTCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGC                                                                                         <  3:86914/135‑1                                                  CTACCGAACAAAATGCATTGACCTACCACGCCGTCCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCT                                                                                     >  5:59529/1‑136                                                  CTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGCCGAGGGCAGGATGACTTTCATGCCCGCGTC                                                                                      >  1:149627/1‑135                                                      CGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGCCTTTCATGCCCGCGTCTGCC                                                                                  >  8:156136/1‑135                                                      CGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCC                                                                                  >  2:48543/1‑135                                                      CGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCC                                                                                  >  2:44834/1‑135                                                      CGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCC                                                                                  >  8:241721/1‑135                                                        AACAAAATCCATTGACCTACCACGCCGCCCGCAGCCTTACTGGATACATAAAATAAAACCAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGC                                                                                <  7:340823/135‑1                                                             AATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCC                                                                          >  6:15274/1‑136                                                                GCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCG                                                                         >  4:281208/1‑134                                                                     GACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAG                                                                   >  3:408480/1‑135                                                                     GACCTACCACGCCGACCGCAGCCTTAATGGATACATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGG                                                                  >  6:354611/1‑136                                                                          ACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGACTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTC                                                              >  3:207946/1‑135                                                                           CCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCG                                                             <  2:377179/135‑1                                                                           CCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCG                                                             <  5:186325/135‑1                                                                           CCACGCCGACCGCAGCCTAAATGGAACCATTAAATAACAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCG                                                             <  2:150031/135‑1                                                                               GCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATA                                                        <  6:395822/136‑1                                                                                   ACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCG                                                     >  5:321858/1‑135                                                                                   ACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTATGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCG                                                     >  5:61880/1‑135                                                                                   ACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGG                                                                                                          >  8:227705/1‑82                                                                                   ACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGG                                                                                                          <  7:227705/82‑1                                                                                    CCGCAGCCTTAATGCATCCATAAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCATACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCA                                                   <  5:221396/136‑1                                                                                     CGCAGACTAAATGGATCCATTAAATAAAAACAAAAACCGTAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGAAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCA                                                   <  2:374117/135‑1                                                                                         GCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGT                                               <  2:158434/135‑1                                                                                         GCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGT                                               <  3:247026/135‑1                                                                                                TGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATT                                         <  7:188927/134‑1                                                                                                       ATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCT                                 >  8:259295/1‑135                                                                                                       ATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCT                                 >  3:282253/1‑135                                                                                                       ATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCT                                 >  8:125223/1‑135                                                                                                           AATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGC                              >  2:167169/1‑134                                                                                                                      AACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCATGATCGCTT                  <  4:73430/135‑1                                                                                                                        CCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCATGATCGCTT                  <  7:192134/133‑1                                                                                                                        CCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCATGATCGCTT                  >  8:192134/1‑133                                                                                                                            GAGCGCCGCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCATGAGCGCTTCGGCGC            <  7:163240/135‑1                                                                                                                            GAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCATGATCGCTTCGGCGC            <  1:44834/135‑1                                                                                                                            GAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCATGATCGCTTCGGCGC            <  8:293057/135‑1                                                                                                                            GAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGGTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCATGATCGCTTCGGCGC            <  2:162925/135‑1                                                                                                                               AGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCATGATCGCTTCGGCGCGGGC        >  6:241107/1‑136                                                                                                                                      CGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTCCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCATGATCGCTTCGGCGCGGGCACCCTG  <  1:258400/135‑1                                                                                                                                      ||||||||                                                                                                                                GCCCCATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  ACB143/4257202‑4257334 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCATGATCGCTTCGGCGCGGGCACCCTG  >  ACB143/4257335‑4257461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.