| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | ACB143 | 4,759,572 | C→T | N16N (AAC→AAT) | ligM_KToptimized → | sequence generated by OPTIMIZER for KT2440 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 4,759,572 | 0 | C | T | 98.7% | 266.7 / ‑6.7 | 74 | N16N (AAC→AAT) | ligM_KToptimized | sequence generated by OPTIMIZER for KT2440 |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (47/26); minor base A (0/1); total (47/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCAC > ACB143/4759448‑4759701 | atcatcGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCACCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACAGTTGAAAt > 5:178083/1‑136 (MQ=255) atcGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGt > 1:188257/1‑135 (MQ=255) tcGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTg < 5:233606/136‑1 (MQ=255) cTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCg > 1:331892/1‑134 (MQ=255) cGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTaa > 1:125578/1‑135 (MQ=255) tgGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTg < 6:278568/135‑1 (MQ=255) ggAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTg < 2:400065/134‑1 (MQ=255) ggAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGgc > 6:146255/1‑136 (MQ=255) taaTTGGGAGCGTATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGgc < 1:391526/134‑1 (MQ=255) gAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGAGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGgc < 6:191316/135‑1 (MQ=255) gAATTGTCAGCGGATAACATTATCACACCAACACATAGATTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGgc < 5:259242/135‑1 (MQ=255) aaTTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGTCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGgcgc > 6:272585/1‑136 (MQ=255) aaTTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGgcgc > 5:41230/1‑136 (MQ=255) aaTTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGgcgc > 6:336294/1‑136 (MQ=255) gCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGt > 8:237226/1‑134 (MQ=255) gCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGt > 1:381629/1‑134 (MQ=255) gCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTc > 5:79690/1‑135 (MQ=255) gCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTAGACATGAGCGCCCCGACTACCCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGt > 4:352998/1‑134 (MQ=255) ggATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTa > 2:406026/1‑135 (MQ=255) ggATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTAc > 6:179588/1‑136 (MQ=255) aCAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCgg > 7:238231/1‑135 (MQ=255) cAATTTCACACCAACACATAGTGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGgt < 1:381351/135‑1 (MQ=255) cAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGgt < 1:372375/135‑1 (MQ=255) tttCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGgtcgtc < 5:359733/136‑1 (MQ=255) cacaCCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGTCGACTACGTCTACCCGGTCGTcgc > 1:222543/1‑135 (MQ=255) acacCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTcgcg < 1:365082/135‑1 (MQ=255) cacCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCgcgc < 1:45185/135‑1 (MQ=255) cacCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCACATTGGCGCCTACGTCTACCCGGTCGTcgcg < 7:288238/134‑1 (MQ=255) acaTAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTtc < 6:25295/136‑1 (MQ=255) caTAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGtt > 7:82394/1‑134 (MQ=255) aTAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTtct > 3:330175/1‑135 (MQ=255) gagTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTaa < 5:91374/134‑1 (MQ=255) agTTTCGGAGTTATACATCAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTaa < 4:5484/134‑1 (MQ=255) agTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTaa < 7:43175/134‑1 (MQ=255) gTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAAc > 4:245084/1‑135 (MQ=255) gTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAAc > 3:7532/1‑135 (MQ=255) gTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAAc > 3:136578/1‑135 (MQ=255) gTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACt < 6:371586/136‑1 (MQ=255) gTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACt < 6:22790/136‑1 (MQ=255) gTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACt < 5:396405/136‑1 (MQ=255) tttAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAAc < 7:235315/134‑1 (MQ=255) tAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTgg > 2:376394/1‑135 (MQ=255) gaggTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCTTAACTCACAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTATCT‑ACTAGCGTa > 7:263394/1‑135 (MQ=255) gaggTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACAGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCAAGAGTTCTCTAACTGGCGt > 5:299660/1‑135 (MQ=255) aggTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGt < 1:408799/134‑1 (MQ=255) aggTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTAc > 6:145883/1‑136 (MQ=255) taCATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGa > 8:12168/1‑134 (MQ=255) cATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGaa > 6:26848/1‑133 (MQ=255) cATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACa > 8:379406/1‑135 (MQ=255) cATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACa > 1:241243/1‑135 (MQ=255) cATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAg > 5:62256/1‑136 (MQ=255) ccccGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCtt > 1:204560/1‑135 (MQ=255) cccGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTg > 1:327354/1‑135 (MQ=255) cccGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTg > 4:269571/1‑135 (MQ=255) cccGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTg > 3:34075/1‑135 (MQ=255) cccGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGACAGAGTTCTCTAACTGTCGTTCCCAACACTGGGCTTg > 3:313596/1‑135 (MQ=255) cccGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGAAATACAGTTGAAATGTTGCGTAACTCACAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTg > 7:152155/1‑135 (MQ=255) cGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTaa > 8:287070/1‑109 (MQ=255) cGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTaa < 7:287070/109‑1 (MQ=255) cTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCa > 4:257386/1‑135 (MQ=255) aaCCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCacc > 8:323623/1‑133 (MQ=255) aGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGc > 7:80774/1‑135 (MQ=255) aGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCt > 6:108562/1‑136 (MQ=255) gCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGtt < 3:264695/135‑1 (MQ=255) gCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCAACGTCTACCCGGTCCTCGCGCCAGACTTCTCTAACTTCCGTACCCAACAGTGGGCTTGGCGCAACTCCGCCGGGCTGTTc > 5:202483/1‑136 (MQ=255) cTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGtt > 3:211207/1‑134 (MQ=255) cTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGGTc > 3:217560/1‑135 (MQ=255) cTGGCCGCCGGCGGCAATACCGGTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGCGTGCTCTAACTGGCGGACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGttg > 4:390689/1‑134 (MQ=255) ttgccgccGTCGTAAATAACGTTGAAATGTTGAGTAACTCCCAGATTGGAGCCTACGTCTACACGGTCGTCGCGCCAGAGTTCTCTAACTGTCGTACCGAACAGTGTGCTTGGCGCAACTCCGCCGTGCTGTTCg < 3:227808/133‑1 (MQ=255) cggcggCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAAcc < 5:127761/136‑1 (MQ=255) gcggcAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTTCCGAACAGTGGGCTTGGCGCACCGCCGCCGGGCTGTTCGACCAAAccc > 4:325550/1‑135 (MQ=255) gcggcAAAACCGTTGAAATGATGCGGAACTCCCAGATTTGCGCCTAAGTCTACCCGGTCGGCGCGGCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACcca < 5:8934/136‑1 (MQ=255) cggcAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACAAAACccac > 6:275697/1‑136 (MQ=255) cggcAATACCGTTGAAATCTTGCGTAACTCCCAGATTGGCGCCTCCGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACcca < 1:142287/135‑1 (MQ=255) | ATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCAC > ACB143/4759448‑4759701 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |