| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | ACB143 | 2,626,199 | Δ2 bp | coding (75‑76/273 nt) | hupB_2 → | DNA‑binding protein HU‑beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 2,626,199 | 0 | G | . | 97.9% | 217.4 / ‑1.5 | 48 | coding (75/273 nt) | hupB_2 | DNA‑binding protein HU‑beta |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base . (22/25); total (23/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.97e-01 | |||||||||||
| * | ACB143 | 2,626,200 | 0 | G | . | 95.8% | 207.8 / 1.8 | 48 | coding (76/273 nt) | hupB_2 | DNA‑binding protein HU‑beta |
| Reads supporting (aligned to +/- strand): ref base G (2/0); new base . (21/25); total (23/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAAACAAACTCAATAGAGAAATAAGGGGACTTAGAGTGAACAAGTCGGAACTGATTGACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCTGGACGCAGTCATCGAATCCGTCACCGGCGCCCTGAAGCAAGGTGACGATGTGGTACTGGTTGGCTTTGGTACCTTCTCGGTCAAGGAGCGCGCCGAGCGTACTGGCCGCAACCCGCAAACCGGCAAGGC > ACB143/2626090‑2626327 || gAAACAAACTCAATAGAGAAATAAGGGGACTTAGAGTGAACAAGTCGGTACTGATTGACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGgc < 7:237343/135‑1 (MQ=255)gAAACAAACTCAATAGAGAAATAAGGGGACTTAGAGTGAACAAGTCGGAACTGATTGACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGgcg > 8:165440/1‑136 (MQ=255) aacaaacTCAATAGAGAAATAAGGGGACTTAGAGTGAACAAGTCGGAACTGATTGACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGGCGcc > 8:197485/1‑136 (MQ=255) acTCAATAGAGAAATAAGGGGACTTAGAGTGAACAAGTCGGAACTGATTGACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGGCGCCCTGaa < 8:151655/136‑1 (MQ=255) cAATAGAGAAATAAGGGGACTTAGAGTGAACAAGTCGGAACTGATTGACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGGCGCCCTGAAGc > 1:62898/1‑135 (MQ=255) cAATAGAGAAATAAGGGGACTTAGAGTGAACAAGTCGGAACTGATTGACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGGCGCCCTGAAGc > 4:25581/1‑135 (MQ=255) gagaAATAAGGGGACTTAGAGTGAACAAGTCGGAACTGATTGACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGGCGCCCTGAAGCAAGGTg < 7:197485/136‑1 (MQ=255) gagaAATAAGGGGACTTAGAGTGAACAAGTCGGAACTGATTGACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGGCGCCCTGAAGCAAGGTg < 6:88120/136‑1 (MQ=255) gagaAATAAGGGGACTTAGAGTGAACAAGTCGGAACTGATTGACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGGCGCCCTGAAGCAAGGTg < 6:224378/136‑1 (MQ=255) agaAATAAGGGGACTTAGAGTGAACAAGTCGGAACTGATTGACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGGCGCCCTGAAGCAAGGTGa < 3:272057/136‑1 (MQ=255) aaTACGGGGACTTAGAGTGAACAAGTCGGAACTGATTGACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGGCGCCCTGAAGCAAGGTGACg < 8:57308/135‑1 (MQ=255) aaTAAGGGGACTTAGAGTGAACAAGTCGGAACTGATTGACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGGCGCCCTGAAGCAAGGTGACGa > 6:42688/1‑136 (MQ=255) tAAGGGGACTTAGAGTGAACAAGTCGGAACTGATTGACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCCGCGCCCTGAAGCAAGGTGACGAtg > 1:163851/1‑136 (MQ=255) ggggACTTAGAGTGAACAAGTCGGAACTGATTGACGCTATCGCCGCATCTGCAGACCTCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACTCAGGCATCGCATAAGTCACCGGCGCCCGGAAGCAAGGTGACGATGTgg > 1:135682/1‑136 (MQ=255) cTTAGAGTGAACAAGTCGGAACTGATTGACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGGCGCCCTGAAGCAAGGTGACGATGTGGTACTg < 2:170179/136‑1 (MQ=255) tAGAGTGAACAAGTCGGAACTGATTGACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGGCGCCCTGAAGCAAGGTGACGATGTGGTACTggg > 3:42307/1‑135 (MQ=255) agagTGAACAAGTCGGAACTGATTGACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGgc > 8:288527/1‑103 (MQ=255) agagTGAACAAGTCGGAACTGATTGACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGgc < 7:288527/103‑1 (MQ=255) agTGAACAAGTCGGAACTGATTGACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGGCGCCCTGAAGCAAGGTGACGATGTGGTACTGGTTgg < 4:347568/136‑1 (MQ=255) aaCAAGTCGGAACTGATTGACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGGCGCCCTGAAGCAAGGTg > 8:238108/1‑113 (MQ=255) aaCAAGTCGGAACTGATTGACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGGCGCCCTGAAGCAAGGTg < 7:238108/113‑1 (MQ=255) aaCAAGTCGGAACTGATTGACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGGCGCCCTGAAGCAAGGTGACGATGTGGTACTGGTTGGCttt < 4:148173/136‑1 (MQ=255) gTCGGAACTGATTGACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGGCGCCCTGAAGCAAGGTGACGATGTGGTACTGGTTGGCTTTGGTAc < 8:295918/136‑1 (MQ=255) gTCGGAACTGATTGACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGGCGCCCTGAAGCAAGGTGACGATGTGGTACTGGTTGGCTTTGGTAc < 2:206306/136‑1 (MQ=255) ggAACTGATTGACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGGCGCCCTGAAGCAAGGTGACGATGTGGTACTGGTTGGCTTTGGTACCt < 2:339548/135‑1 (MQ=255) ggAACTGATTGACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGGCGCCCTGAAGCAAGGTGACGATGTGGTACTGGTTGGCTTTGGTACCt < 2:181849/135‑1 (MQ=255) ggAACTGATTGACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGGCGCCCTGAAGCAAGGTGACGATGTGGTACTGGTTGGCTTTGGTACCt < 7:163980/135‑1 (MQ=255) ggAACTGATTGACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGGCGCCCTGAAGCAAGGTGACGATGTGGTACTGGTTGGCTTTGGTACCt < 2:6578/135‑1 (MQ=255) ggAACTGATTGACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGGCGCCCTGAAGCAAGGTGACGATGTGGTACTGGTTGGCTTTGGTACCt < 3:323483/135‑1 (MQ=255) ggAACTGATTGACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGGCGCCCTGAAGCAAGGTGACGATGTGGTACTGGTTGGCTTTGGTACCt < 4:180658/135‑1 (MQ=255) gAACTGATTGACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGGCGCCCTGAATCCAGGTGACGATGTGGTACTGGTTGGCTTTGGTACCTtc > 5:240863/1‑136 (MQ=255) gAACTGATTGACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGC‑‑GACGCAGTCATCGAATACCTCACCGGCGCCCAGAAGCAAGGTGCAGATGTGGTCCTGGTTGGCGTTGGGACCTtc > 6:221062/1‑136 (MQ=255) tGATTGACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCCTCGAATCCCTCACCGGCCCCCTGAAGCAAGCTGACGATGTGGTACTGGTTGGCGTTGGTACCTTCACgc > 3:158691/1‑135 (MQ=255) tGATTGACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGGCGCCCTGAAGCAAGGTGACGATGTGGTACTGGTTGGCTTTGGTACCTTCTCgg > 8:276263/1‑136 (MQ=255) gACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCa < 4:316795/74‑1 (MQ=255) gACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCa > 3:316795/1‑74 (MQ=255) gACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGGCGCCCTGAAGCAAGGTGACGATGTGGTACTGGTTGGCTTTGGTACCTTCTCGGTCAAg > 5:94455/1‑136 (MQ=255) gACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGGCGCCCTGAAGCAAGGTGACGATGTGGTACTGGTTGGCTTTGGTACCTTCTCGGTCAAg > 7:50738/1‑136 (MQ=255) gACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGGCGCCCTGAAGCAAGGTGACGATGTGGTACTGGTTGGCTTTGGTACCTTCTCGGTCAAg > 8:133385/1‑136 (MQ=255) aCGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGGCGCCCTGAAGCAAGGTGACGATGTGGTACTGGTTGGCTTTGGTACCTTCTCGGTCAAg < 5:10223/135‑1 (MQ=255) tCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGGCGCCCTGAAGCAAGGTGACGATGTGGTACTGGTTGGCTTTGGTACCTTCTCGGTCAAGGAgcgcg < 7:170588/136‑1 (MQ=255) ccgcATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGGCGCCCTGAAGCAAGGTGACGATGTGGTACTGGTTGGCTTTGGTACCTTCTCGGTCAAGGAGCGCGCCg > 1:77317/1‑136 (MQ=255) ccgcATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGGCGCCCTGAAGCAAGGTGACGATGTGGTACTGGTTGGCTTTGGTACCTTCTCGGTCAAGGAGCGCGCCg > 3:140145/1‑136 (MQ=255) gACATCCCGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGGCGCCCTGAAGCAAGGTGACGATGTGGTACTGGTTGGCTTTGGTACCTTCTCGGTCAAGGAGCGCGCCGAGCGTACTGGc < 1:200601/136‑1 (MQ=255) ccGAAAGCTGTAGCCGGCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGGCGCCCTGAAGCAAGGTGACGATGTGGTACTGGTTGGCTTTGGTACCTTCTCGGTCAAGGAGCGCGCCGAGCGTACTGGCCGCAAc > 6:112466/1‑136 (MQ=255) tAGCGGTCCGTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGTCGCCCTGAAGCAAGGTGACGATGTGGTACTGGTTGGCTTTGGTACCTTCTCGGTCAAGGAGCGCGCCGAGCGTACTGGCCGCAACCCGCAAACCg < 6:191901/136‑1 (MQ=255) ccggccgTGCGCT‑‑ACGCAGTCATCGAATCCGTCACCGGCGCCCTGAAGCAAGGTGACGATGTGGTACTGGTTGGCTTTGGTACCTTCTAGGTCAAGGAGCGCGCCGAGCGTACTGGCCGCAACCCGCAAACCggc > 7:86749/1‑135 (MQ=255) tGCGCTGGACGCAGTCATCGAATCCGTCACCGGCGCCCTGAAGCAAGGTGACGATGTGGTACTGGTTGGCTTTGGTACCTTCTCGGTCAAGGAGCGCGCCGAGCGTACTGGCCGCAACCCGCAAACCggcaaggc > 3:96668/1‑135 (MQ=255) || GAAACAAACTCAATAGAGAAATAAGGGGACTTAGAGTGAACAAGTCGGAACTGATTGACGCTATCGCCGCATCTGCAGACATCCCGAAAGCTGTAGCCGGCCGTGCGCTGGACGCAGTCATCGAATCCGTCACCGGCGCCCTGAAGCAAGGTGACGATGTGGTACTGGTTGGCTTTGGTACCTTCTCGGTCAAGGAGCGCGCCGAGCGTACTGGCCGCAACCCGCAAACCGGCAAGGC > ACB143/2626090‑2626327 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |