| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| JC | ACB143 | 5,390,915 | (GCTCA)1→2 | coding (1009/1666 nt) | ligH → | 10‑formyltetrahydrofolate synthetase |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | ACB143 | = 5390910 | 0 (0.000) | 40 (0.730) +GCTCA |
23/242 | 0.9 | 100% | coding (1004/1666 nt) | ligH | 10‑formyltetrahydrofolate synthetase |
| ? | ACB143 | 5390911 = | 0 (0.000) | coding (1005/1666 nt) | ligH | 10‑formyltetrahydrofolate synthetase | |||||
CTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGACCTGGGTGCGGAGAAGTTCTTCGACATCAAATGCCGCAAGGCGGGCCTCAAGCCCTCGGCCGCAGTGATCGTGGCGACGATCCGCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ > ACB143/5390777‑5390910‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCTCAAGATGCATGGCGGCGTCGACAAGGCCGATCTCGGCACGGCGAACCCGGAGGCGGTCCGCAAGGGCGGCGTCAATCTCGCTCGCCACATCGAGAATGTCCGCCAGTTCGGCGTGCCGGTGGTGGTCGCG > ACB143/5390911‑5391043 ||||| CTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGACCTGGGTGCGGAGAAGTTCTTCGACATCAAATGCCGCAAGGCGGGCCTCAAGCCCTCGGCCGCAGTGATCGTGGCGACGATCCGCGCGC > 1:215951/1‑136CTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGACCTGGGTGCGGAGAAGTTCTTCGACATCAAATGCCGCAAGGCGGGCCTCAAGCCCTCGGCCGCAGTGATCGTGGCGACGATCCGCGCGC > 1:245488/1‑136CTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGACCTGGGTGCGGAGAAGTTCTTCGACATCAAATGCCGCAAGGCGGGCCTCAAGCCCTCGGCCGCAGTGATCGTGGCGACGATCCGCGCGC > 1:53176/1‑136CTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGACCTGGGTGCGGAGAAGTTCTTCGACATCAAATGCCGCAAGGCGGGCCTCAAGCCCTCGGCCGCAGTGATCGTGGCGACGATCCGCGCGC > 3:28058/1‑136CTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGACCTGGGTGCGGAGAAGTTCTTCGACATCAAATGCCGCAAGGCGGGCATCAAGCCCTCGGACGCAGTGATCGTGGCGACGATCCGCGCGC > 7:248083/1‑136 AAGATCGCCGATTATGT‑GTGACGGAAGCCGGCTTCGGTGCCGACCTGGGTGCGGAGAAGTTCTTCGACATCAAATGCCGCAAGGCGGGCCTCAAGCCCTCGGCCGCAGTGATCGTGGCGACGATCCGCGCGCTCAG > 3:91668/1‑136 AGATCGCCGATTATGTGGTGACGGAAGCAGGCTTCGGTGCCGACCTGGGTGCGGAGAAGTTCTTCGACATCAAATGCCGCAAGGCGGGCCTCAAGCCCTCGGCCGCAGTGATCGTGGCGACGATCCGCGCGCTCAG < 8:336476/136‑1 GATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGACCTGGGTGCGGAGAAGTTCTTCGACATCAAATGCCGCAAGGCGGGCCTCAAGCCCTCGGCCGCAGTGATCGTGGCGACGATCCGCGCGCTCAGC < 2:215951/136‑1 GATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGACCTGGGTGCGGAGAAGTTCTTCGACATCAAATGCCGCAAGGCGGGCCTCAAGCCCTCGGCCGCAGTGATCGTGGCGACGATCCGCGCGCTCAGC < 6:75999/136‑1 CGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCTACCTGGGTGCGGAGAAGTTCTTCGACATCAAATGCCGCAAGGCGGGCCTCAAGCCCTCGGCCGCAGTGATCGTGGCGACGATCCGCGCGCTCAGCTC < 3:317892/135‑1 ATGTGGTGACGGAAGCCGGCTTCGGTGCCGACCTGGGTGCGGAGAAGTTCTTCGACATCAAATGCCGCAAGGCGGGCCTCAAGCCCTCGGCCGCAGTGATCGTGGCGACGATCCGCGCGCTCAGCTCAAGATGCAT > 6:60866/1‑136 GAAGCCGGCTTCGGTGCCGACCTGGGTGCGGAGAAGTTCTTCGACATCAAATGCCGCAAGGCGGGCCTCAAGCCCTCGGCCGCAGTGATCGTGGCGACGATCCGCGCGCTCAGCTCAAGATGCATGGCGGCGTCGA > 5:134525/1‑136 CCGGCTTCGGTGCCGACCTGGGTGCGGAGAAGTTCTTCGACATCAAATGCCGCAAGGCGGGCCTCAAGCCCTCGGCCGCAGTGATCGTGGCGACGATCCGCGCGCTCAGCTCAAGATGCATGGCGGCGTCGACAAG < 3:321768/136‑1 CCGACCTGGGTGCGGAGAAGTTCTTCGACATCAAATGCCGCAAGGCGGGCCTCAAGCCCTCGGCCGCAGTGATCGTGGCGACGATCCGCGCGCTCAGCTCAAGATGCATGGCGGCGTCGACAAGGCCGATCTCGG > 7:184948/1‑135 GACCTGGGTGCGGAGAAGTTCTTCGACATCAAATGCCGCAAGGCGGGCCTCAAGCCCTCGGCCGCAGTGATCGTGGCGACGATCCGCGCGCTCAGCTCTAGATGCATGGCGGCGTCGACTAGGCCGATCTCGGCAg < 5:137608/136‑2 caTGGGTGCGGAGAAGTTCTTCGACATCAAATGCCGCAAGGCGGGCCTCAAGCCCTCCGCCGCAGTGATCGTGGCGACGATCCGCGCGCTCAGCTCAAGATGCAATGCGGCGTCGACAAGGCCGATCTCGGCACtg > 4:185577/3‑134 TGGGTGCGGAGAAGTTCTTCGACATCAAATGCCGCAAGGCGGGCCTCAAGCCCTCGGCCGCAGTGATCGTGGCGACGATCCGCGCGCTCAGCTCAAGATGCATGGCGGCGTCGACAAGGCCGATCTCGGCACGGC > 1:71761/1‑135 TGGGTGCGGAGAAGTTCTTCGACATCAAATGCCGCAAGGCGGGCCTCAAGCCCTCGGCCGCAGTGATCGTGGCGACGATCCGCGCGCTCAGC < 3:294702/92‑1 TGGGTGCGGAGAAGTTCTTCGACATCAAATGCCGCAAGGCGGGCCTCAAGCCCTCGGCCGCAGTGATCGTGGCGACGATCCGCGCGCTCAGC > 4:294702/1‑92 GGAGAAGTTCTTCGACATCAAATGCCGCAAGGCGGGCCTCAAGCCCTCGGCCGCAGTGATCGTGGCGACGATCCGCGCGCTCAGCTCAAGATGCATGGCGGCGTCGACAAGGCCGATCTCGGCACGGCGAACCCGG < 6:298267/136‑1 acAAGTTCTTCGACATCAAATGACTCAAGGCGGCCCTCAAGCCCTCGGCCGCAGTGATCGTGGCGACGATCCGCGCGCTCAGCTCAAGATGCATGGCGGCGTCGACAAGGCCGATCTCGGCACGGCGAACCCGGAG > 8:320072/3‑136 AGAAGTTCTTCGACATCAAATGCCGCAAGGCGGGCCTCAAGCCCTCGGCCGCAGTGATCGTGGCGACGATCCGCGCGCTCAGCTCAAGATGCATGGCGGCGTCGACAAGGCCGATCTCGGCACGGCGAACCCGGAG < 7:53425/136‑1 CTTCGACATCAAATGCCGCAAGGCGGGCCTCAAGCCCTCGGCCCAACTGATCGTGGCGACGATCCGCGCGCTCAGCTCAAGATGCATGGCGGCGACCCCAAGGCCGATCTCGGCACGGCGAACCCGGAGGCGGTCC > 1:164178/1‑136 ACATCAAATGCCGCAAGGCGGGCCTCAAGCCCTCGGCCGCAGTGATCGTGGCGACGATCCGCGCGCTCAGCTCAAGATGCATGGCGGCGTCGACAAGGCCGATCTCGGCACGGCGAACCCGGAGGCGGTCCGCAAG < 5:6673/136‑1 AATGCCGCAAGGCGGGCCTCAAGCCCTCGGCCGCAGTGATCGTGGCGACGATCCGCGCGCTCAGCTCAAGATGCATGGCGGCGTCGACAAGGCCGATCTCGGCACGGCGAACCCGGAGGCGGTCCGCAAGGGCGGC > 4:117590/1‑136 AATGCCGCAAGGCGGGCCTCAAGCCCTCGGCCGCAGTGATCGTGGCGACGATCCGCGCGCTCAGCTCAAGATGCATGGCGGCGTCGACAAGGCCGATCTCGGCACGGCGAACCCGGAGGCGGTCCGCAAGGGCGGC > 4:156521/1‑136 AATGCCGCAAGGCGGGCCTCAAGCCCTCGGCCGCAGTGATCGTGGCGACGATCCGCGCGCTCAGCTCAAGATGCATGGCGGCGTCGACAAGGCCGATCTCGGCACGGCGAACCCGGAGGCGGTCCGCAAGGGCGGC > 8:194502/1‑136 AATGCCGCAAGGCGGGCCTCAAGCCCTCGGCCGCAGTGATCGTGGCGACGATCCGCGCGCTCAGCTCAAGATGCATGGCGGCGTCGACAAGGCCGATCTCGGCACGGCGAACCCGGAGGCGGTCCGCAAGGGCGGC > 2:269892/1‑136 GGCCTCAAGCCCTCGGCCGCAGTGATCGGGGCGACGATCCGCGCGCTCAGCTCAAGATGCATGGCGGCGTCGACAAGGCCGATCTCGGCACGGCGAACCCGGAGGCGGTCCGCAAGGGCGGCGTCAATCTCGCTCG < 7:249893/136‑1 GGCCTCAAGCCCGCGGCCGCAGTGATCGTTGCGACGATCCGCGCGCTCAGCTCAAGATGCATGGCGGAGTCGACAAGGCAGATCTCGTCACGGCGAACCCGGAGGAGGTCCGCAAGGGCGGCGTCAATCTCGCTCG < 6:346516/136‑1 TCAAGCCCTCGGCCGCAGTGATCGTGGCGACGATCCGCGCGCTCAGCTCAAGATGCATGGCGGCGTCGACAAGGCCGATCTCGGCACGGCGAACCCGGAGGCGGTCCGCAAGGGCGGCGTCAATCTCGCTCGCCA > 1:193283/1‑135 TCAAGCCCTCGGCCGCAGTGATCGTGGCGACGATCCGCGCGCTCAGCTCAAGATGCATGGCGGCGTCGACAAGGCCGATCTCGGCACGGCGAACCCGGAGGCGGTCCGCAAGGGCGGCGTCAATCTCGCTCGCCA > 7:155272/1‑135 TCAAGCCCTCGGCCGCAGTGATCGTGGCGACGATCCGCGCGCTCAGCTCAAGATGCATGGCGGCGTCGACAAGGCCGATCTCGGCACGGCGAACCCGGAGGCGGTCCGCAAGGGCGGCGTCAATCTCGCTCGCCA > 3:196930/1‑135 CCGCAGTGATCGTGGCGACGATCCGCGCGCTCAGCTCAAGATGCATGGCGGCGTCGACAAGGCCGATCTCGGCACGGCGAACCCGGAGGCGGTCCGCAAGGGCGGCGTCAATCTCGCTCGCCACATCGAGAATGTC > 2:228734/1‑136 CCGCAGTGATCGTGGCGACGATCCGCGCGCTCAGCTCAAGATGCATGGCGGCGTCGACAAGGCCGATCTCGGCACGGCGAACCCGGAGGCGGTCCGCAAGGGCGGCGTCAATCTCGCTCGCCACATCGAGAATGTC > 3:295834/1‑136 GTGATCGTGGCGACGATCCGCGCGCTCAGCTCAAGATGCATGGCGGCGTCGACAAGGCCGATCTCGGCACGGCGAACCCGGAGGCGGTCCGCAAGGGCGGCGTCAATCTCGCTCGCCACATCGAGAATGTCCGCCA < 7:305591/136‑1 GATCGTGGCGACGATCCGCGCGCTCAGCTCAAGATGCATGGCGGCGTCGACAAGGCCGATCTCGGCACGGCGAACCCGGAGGCGGTCCGCAAGGGCGGCGTCAATCTCGCTCGCCACATCGAGCATGTCCGCCcgg > 6:218854/1‑133 GTGGCGACGATCCGCGCGCTCAGCTCAAGATGCATGGCGGCGTCGACAAGGCCGATCTCGGCACGGCGAACCCGGAGGCGGTCCGCAAGGGCGGCGTCAATCTCGCTCGCCACATCGAGAATGTCCGCCAGTTCGG > 4:1720/1‑136 GTGGCGACGATCCGCGCGCTCAGCTCAAGATGCATGGCGGCGTCGACAAGGCCGATCTCGGCACGGCGAACCCGGAGGCGGTCCGCAAGGGCGGCGTCAATCTCGCTCGCCACATCGAGAATGTCCGCCAGTTCG < 4:91668/135‑1 CGATCCGCGCGCTCAGCTCAAGATGCATGGCGGCGTCGACAAGGCCGATCTCGGCACGGCGAACCCGGAGGCGGTCCGCAAGGGCGGCGTCAATCTCGCTCGCCACATCGAGAATGTCCGCCAGTTCGGCGTGCCG < 2:259732/136‑1 CGCGCGCTCAGCTCAAGATGCATGGCGGCGTCGACAAGGCCGATCTCGGCACGGCGAACCCGGAGGCGGTCCGCAAGGGCGGCGTCAATCTCGCTCGCCACATCGAGAATGTCCGCCAGTTCGGCGTGCCGGTGGT < 7:118936/136‑1 GCTCAGCTCAAGATGCATGGCGGCGTCGACAAGGCCGATCTCGGCACGGCGAACCCGGAGGCGGTCCGCAAGGGCGGCGTCAATCTCGCTCGCCACATCGAGAATGTCCGCCAGTTCGGCGTGCCGGTGGTGGTCG > 2:260867/1‑136 GCTCAGCTCAAGATGCATGGCGGCGTCGACAAGGCCGATCTCGGCACGGCGAACCCGGAGGCGGTCCGCAAGGGCGGCGTCAATCTCGCTCGCCACATCGAGAATGTCCGCCAGTTCGGCGTGCCGGTGGTGGTCG < 7:320072/136‑1 AGCTCAAGATGCATGGCGGCGTCGACAAGGCCGATCTCGGCACGGCGAACCCGGAGGCGGTCCGCAAGGGCGGCGTCAATCTCGCTCGCCACATCGAGAATGTCCGCCAGTTCGGCGTGCCGGTGGTGGTCGCG < 3:336636/134‑1 ||||| CTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGACCTGGGTGCGGAGAAGTTCTTCGACATCAAATGCCGCAAGGCGGGCCTCAAGCCCTCGGCCGCAGTGATCGTGGCGACGATCCGCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ > ACB143/5390777‑5390910‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCTCAAGATGCATGGCGGCGTCGACAAGGCCGATCTCGGCACGGCGAACCCGGAGGCGGTCCGCAAGGGCGGCGTCAATCTCGCTCGCCACATCGAGAATGTCCGCCAGTTCGGCGTGCCGGTGGTGGTCGCG > ACB143/5390911‑5391043 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |