Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,277,0290CG32.7% 91.6 / 15.3 56F279L (TTC→TTG) hbd3‑hydroxybutyryl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (10/25);  new base G (17/0);  total (31/25)
Fisher's exact test for biased strand distribution p-value = 4.34e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GATCGGCCTGGACACCCTGCTGGCAATCATGGAGGCCTTCCACGAAGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGC‑AAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTGTGCCAACTTTGAGGAGCGACGTGACCGGGCCATGGCATTGTTTGCCGAAAAGGGCTTTGGCCAGGTCAGCATGCGCGAGCT  >  ACB143/4276894‑4277163
                                                                                                                                       |                                                                                                                                       
gctCGGCCTGCACACCCGGCTGGAAATCATGGCGGCCTCCCACGAAGGCTTCAACGACAGAAAATACCGCCCTGCTCCACTGCTCACGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTttc                                                                                                                                         <  8:22840/134‑1 (MQ=255)
 aTCGGCCTGGACCCCCTGCTGCAAATCATGGAGGCCTTCCACGAAGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGTTttc                                                                                                                                         <  7:127569/135‑1 (MQ=255)
 aTCGGCCTGGACACCCTGCTGGCAATCATGGAGGCCTTCCACGAAGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTttc                                                                                                                                         <  8:212462/135‑1 (MQ=255)
    ggCCTGGACACCCTGCTGGCAATCATGGAGGCCTTCCACGAAGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCa                                                                                                                                     >  2:260512/1‑136 (MQ=255)
         ggACACCCTGCTGGCAATCATGGAGGCCTTCCACGAAGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTGTTGACCGAc                                                                                                                                >  5:50784/1‑136 (MQ=255)
         ggACACCCTGCTGGCAATCATGGAGGCCTTCCACGAAGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTGGTCAccaa                                                                                                                                 >  1:272490/1‑133 (MQ=255)
         ggACACCCTGCTGGCAATCATGGAGGCCTTCCACGAAGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTATTCAccaaa                                                                                                                                >  6:345878/1‑133 (MQ=255)
                  gctgGCAATCATGGAGGCCTTCCACGAAGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCAACAAATGAGGAGc                                                                                                                        >  2:188834/1‑135 (MQ=255)
                  gctgGCAATCATGGAGGCCTTCCACGAAGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTGTTGACCTACTGAGgcgc                                                                                                                        >  3:161151/1‑135 (MQ=255)
                  gctgGCAATCATGGAGGCCTTCCACGAAGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTCTTCCCCTAATGGGGTGc                                                                                                                        >  7:45071/1‑135 (MQ=255)
                      gCAATCTGGGAGCCCTTCACCGATGGCTTCAACCACAGCAAAACCCGCCCTCCTCCCCTGCTCAAGTAAATGGCCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGC‑AAgcg                                                                                                                  <  5:6403/136‑1 (MQ=255)
                      gCAATCATGGAGGCCTTCCACGAAGGCTTCAACGACAGAAAATACCGCCCTGCTCCACGGCTCACGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGC‑AAgcg                                                                                                                  <  6:292278/136‑1 (MQ=255)
                           cATGGCGGCCTCCCCCGACGGCTTCAACGACACCAAAACCCCCCCTGCCCCACTGCTCCAGGAAATGGGCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTCCTGAGGAGC‑AAGCGCCATg                                                                                                             <  3:327783/136‑1 (MQ=255)
                                aGGCCTTCCACGACGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGC‑AAGCGCCATGccccc                                                                                                        <  2:251181/136‑1 (MQ=255)
                                     ttCCACGAAGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTttctt                                                                                                                                       >  6:53483/1‑101 (MQ=255)
                                     ttCCACGAAGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTttctt                                                                                                                                       <  5:53483/101‑1 (MQ=255)
                                     ttCCACGAAGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTGTTGACCGAATGAGGAGCAAACCCCCATCCCCCCggc                                                                                                     >  1:320946/1‑134 (MQ=255)
                                       ccACGAAGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGC‑AAGCGCCATGCCCCCGGTCa                                                                                                   <  7:178975/134‑1 (MQ=255)
                                       ccACGAAGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGC‑AAGCGCCATGCCCCCGGTCa                                                                                                   <  7:185225/134‑1 (MQ=255)
                                       ccACGAAGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTGTTACCCGACTGAGGAGC‑CAGCCCCAAGCCCCCGGGCaaa                                                                                                 >  8:127558/1‑135 (MQ=255)
                                         aCGAAGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTGTTCACCTACTGATGAGC‑AAGCGCCAAGCCCCCCGTCAATGc                                                                                               >  8:64047/1‑136 (MQ=255)
                                          cGAAGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGC‑AAGCGCCAAGCCCCCCGTCAATTcc                                                                                              >  7:224943/1‑136 (MQ=255)
                                            aaGGCTTCAACGCCAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGACTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGC‑AAGCGCCATGCCCCCGGTCAATGCCGc                                                                                            <  1:161495/136‑1 (MQ=255)
                                                cTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCAGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGC‑AAGCGCCATGCCCCCGGTCAATGCCGCAATg                                                                                        <  8:245523/136‑1 (MQ=255)
                                                     accaCAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAAGGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGC‑AAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCtg                                                                                   <  4:315263/133‑1 (MQ=255)
                                                     aCGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGC‑AAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCtg                                                                                   <  1:260512/136‑1 (MQ=255)
                                                     aCGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGGCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGC‑AAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCtg                                                                                   <  1:114358/136‑1 (MQ=255)
                                                     aCGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAAGGGGCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGC‑AAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCtg                                                                                   <  2:356568/136‑1 (MQ=255)
                                                     aCGACAGAAAAAACCGCCCTGCTCCACTGCTCAAGCAAAGGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGC‑AAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCtg                                                                                   <  1:42685/136‑1 (MQ=255)
                                                            cAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTGTTGACCAACTGAGGAGC‑AAGCGCCAAGCCCCCGGGCAAAGCCCCCAAGCGCGGTGccca                                                                             >  4:263329/1‑133 (MQ=255)
                                                             aaaTACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTGTTGACCTAATGAGGGGC‑AAGCGCCCTGCCCCCGGGCAATTCCGCCATGCGCAGTGCCAAcg                                                                           >  7:139681/1‑135 (MQ=255)
                                                                      ccggCTCCACTGCTCCAGGACATGGGCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGC‑AAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTGTGCCAACTTTGAGGAGc                                                                  <  6:124455/133‑1 (MQ=255)
                                                                      ccTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGC‑AAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTGTGCCAACTTTGAGGAGc                                                                  <  4:342810/136‑1 (MQ=255)
                                                                       cTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTGTTGACCTACTGAGGAGC‑AAGCGCCATGCCCCCCGTCAATGCCGCAATGCGCGGGGCCAACATTGAGGcgcg                                                                 >  8:98292/1‑136 (MQ=255)
                                                                        tGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTATTCACCTAATGAGGAGC‑CAGCGCCATGCCCCCGGTCAATGCCGCAATTCGCGGTGCCAACTTTGAGGAGCgc                                                                >  1:17567/1‑135 (MQ=255)
                                                                             cagtggtCATGGATATGGTCTCGGCCGTCTGGCTGGGGCGCAAGTGCGGGCGCGGTTTCTTCACCTACTGAGGAGC‑AAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTGTGTCAACTTTGAGGAGCGACGt                                                             <  6:84762/128‑1 (MQ=255)
                                                                             cACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTGTTCACCTTCTGAGGAGC‑CAGCCCCATTCCCCCCGTCAAAGCCGCGCTGCGCGGTGGCAACTTTTAGGAGGGACGTGa                                                           >  6:137626/1‑136 (MQ=255)
                                                                             cACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTGACCTACTGAGGAGC‑AAGCGCCCTGCCCCCCGTCAATGCCGCAATGCGCTGTGCCAACTTTGAGGAGCGACGTGa                                                           >  2:269370/1‑136 (MQ=255)
                                                                             cACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGC‑AAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTGTGCCAACTTTGAGGAGCGACGt                                                             <  7:274242/134‑1 (MQ=255)
                                                                             cACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTAACCTACAGGGGAGC‑AAACCCCATTCCCCCGGTCAATGCCCCAATTCGCTATTGCAACATTGAGGCGCGACCTGa                                                           >  1:269134/1‑136 (MQ=255)
                                                                                  cTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTGTTCCCCGACTGAGGAGC‑AAGCGCCAATCCCCCCGTCAATGCCCCAAAGCGCGGTGCCAACATTTAGGAGCGACCTGACCcgg                                                      >  5:274458/1‑136 (MQ=255)
                                                                                   tCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTGTTCACCTACTTAGGCGC‑AACCGCCATGCCCCCGCTCAATGCCGCAATGCGCGGGGCCCACATTGAGGAGCGCCGGGACCGGGc                                                     >  1:266625/1‑136 (MQ=255)
                                                                                     aaGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTTTTGACCAAATTAGGAGC‑AAACGCCATTCCCCCGGGTAATTCCGCAATTCGCTGTGGCAACTTTGTGGAGCGACGTGACCGGGCCa                                                   >  7:184267/1‑136 (MQ=255)
                                                                                     aaGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGC‑AAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTGTGCCAACTTTGAGGAGCGACGTGACCGGGcc                                                    >  7:262233/1‑135 (MQ=255)
                                                                                     aaGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCGTAACCGACTGAGGAGC‑AAACGCCATGCCCCCCGGCAATGCCCCCAAGCGCTGTGCCCACTTTGATGGGCGACGTGACCGGGcc                                                    >  8:192599/1‑135 (MQ=255)
                                                                                        gAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTTGTGACCGAATGAGGGGC‑AATCGCCAATCCCCCCGGCAAAGCCCCCAATCGCAGTGGCACATTTGAGGAGCGCCCTGACCGGGCCCTgg                                                >  4:27136/1‑136 (MQ=255)
                                                                                               tCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTGTTGACCTACTGAGGAGC‑AAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCGGTGCCAACTTTGAGGAGCGACGTGACCGGGCCATGGCATTGtt                                         >  1:305769/1‑136 (MQ=255)
                                                                                               tCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTGTTCACCGACTGAGGAGC‑AAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTGTGCCAACTTTGAGGAGCGACGTGGCCGGGCCATGGCATTGtt                                         >  8:17921/1‑136 (MQ=255)
                                                                                                     ccggcTGGCTGGGGCGCAAGAGCGGTCGCGGTTTGTTGACCGTCTGAGGGGC‑CAGCGCCCAGCCCCCGGGGAATGGCGCAATGGGCGGTGGCAACTTTGTGGAGCGCCGTGGCCGGGGCATGGGGTTGTTTGGCg                                    >  7:64500/1‑135 (MQ=255)
                                                                                                     ccggcTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGC‑AAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTGTGCCAACTTTGAGGAGCGACGTGACCGGGCCATGGCATTGTTTGCCGa                                   <  7:64047/136‑1 (MQ=255)
                                                                                                     ccggcTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGC‑AAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTGTGCCAACTTTGAGGAGCGACGTGACCGGGCCATGGCATTGTTTGCCGa                                   <  1:188834/136‑1 (MQ=255)
                                                                                                     ccggcTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGC‑AAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTGTGCCAACTTTGAGGAGCGACGTGACCGGGCCATGGCATTGTTTGCCGa                                   <  4:310040/136‑1 (MQ=255)
                                                                                                     ccggcTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGC‑AAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTGTGCCAACTTTGAGGAGCGACGTGACCGGGCCATGGCATTGTTTGCCGa                                   <  4:211675/136‑1 (MQ=255)
                                                                                                     ccggcTGGCTGGGGCGCAAGAGCGGTCGCGGGTTGTTGACCTACTGAGGAGC‑AAGCGCCATGCCCCCCGGCAATGCCGCAATGCGCTGTGGCAACTTTGAGGAGCGACGTGACCGGGCCATGGCATTGTTTGCCg                                    >  3:211675/1‑135 (MQ=255)
                                                                                                            gctggGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGC‑AAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTGTGCCAACTTTGAGGAGCGACGTGACCGGGCCATGGCATTGTTTGCCGAAAAgg                              <  6:249200/134‑1 (MQ=255)
                                                                                                               ggggCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGC‑AAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTGTGCCAACTTTGAGGAGCGACGTGACCGGGCCATGGCATTGTTTGCCGAAAAGGGCttt                         <  3:263329/136‑1 (MQ=255)
                                                                                                                gggCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGC‑AAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTGTGCCAACTTTGAGGAGCGACGTGACCGGGCCATGGCATTGTTTGCCGAAAAGGGCttt                         <  7:221748/135‑1 (MQ=255)
                                                                                                                      aaGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGC‑AAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTGTGCCAACTTTGAGGAGCGACGTGACCGGGCAATGGCATTGTTTGCCGAAAAGGGCTTTGGCCa                    <  1:273005/134‑1 (MQ=255)
                                                                                                                                gcgGTTTGTTAACCTAATGAGGAGC‑AAGCGCCATGCCCCCCCGCAAAGCCGCAAATCGCTGTGCCAACTTTTAGGAGCGACGTGACCGGGCCAAGGCATTGTTTGCCGAAAAGGGCTTTGGCCCGGTaagcaagcc        >  4:59634/1‑135 (MQ=255)
                                                                                                                                gcgGGTTCTTGACCTACTGAGGAGC‑AAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTGTGCCAACTTTGAGGAGGGACGTGTCCGGGCCATGGCATTGTTTTGCGAAAAGGGCTTTGGGCAGGTCAGCATgcg        >  8:113047/1‑136 (MQ=255)
                                                                                                                                   gTTTCTTCACCTACTGAGGAGC‑AAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTGTGCCAACTTTGAGGAGCGACGTGACCGGGCCATGGCATTGTTTGCCGAAAAGGGCTTTGGCCAGGTCAGCATgcgc       >  3:246574/1‑134 (MQ=255)
                                                                                                                                     ttcttcACCTACTGAGGAGC‑AAGCGCCATGCCCCCGGTCAATTCCGCAATGCGCTGTGCCAACTTTGAGGAGCGACGTGACCGGGCCATGGCATTGTTTGCCGAAAAGGGCTTTGGCCAGGTCAGCATGCGCGAGc   >  7:223673/1‑136 (MQ=255)
                                                                                                                                       cttcACCTACTGCGGAGC‑AAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTGTGCCAACTTTGAGGAGCGACGTGACCGGGCCATGGCATTGTTTGCCGAAAAGGGCTTTGGCCAGGTCAGCATGCGCGAGCt  <  1:304878/135‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                       
GATCGGCCTGGACACCCTGCTGGCAATCATGGAGGCCTTCCACGAAGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGC‑AAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTGTGCCAACTTTGAGGAGCGACGTGACCGGGCCATGGCATTGTTTGCCGAAAAGGGCTTTGGCCAGGTCAGCATGCGCGAGCT  >  ACB143/4276894‑4277163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑