Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB143	5,345,970	A→G	L489P (CTG→CCG)	pnp ←	Polyribonucleotide nucleotidyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	5,345,970	0	A	G	100.0%	188.6 / NA	53	L489P (CTG→CCG)	pnp	Polyribonucleotide nucleotidyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/32);  total (21/32)

AGGCGCGCTTCCAGGGCTTGGCCCAGGGCGATTTCCATGATCTCTTCGGTGATGCCGTTGATCTTGATGTCCATCTGCAGCGCGGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCAGGTGGTCTTCGTCACCCAGGATGTCAGTCAGGACTGCGAACTTGTCGCCTTCCTTGACCAGGCCCATGGCGATACCGGCAACCGGCGCCTTCATCGGCACACCGGCGTCCATCAGGGCCAGGGAAGCA  >  ACB143/5345841‑5346098                                                                                                                                  |                                                                                                                                 aGGCGCGCTTCCAGGGCTTGGCCCAGGGCGATTTCCATGATCTCTTCGGTGATGCCGTTGATCTTGATGTCCATCTGCAGCGCGGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGggtggt                                                                                                                            >  5:106966/1‑136 (MQ=255)      cgcTTCCAGGGCTTGGCCCAGGGCGATTTCCATGATCTCTTCGGTGATGCCGTTGATCTTGATGTCCATCTGCAGCGCGGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCg                                                                                                                       >  7:316243/1‑136 (MQ=255)        cTTCCAGGGCTTGGCCCAGGGCGATTTCCATGATCTCTTCGGTGATGCCGTTGATCTTGATGTCCATCTGCAGCGCGGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTc                                                                                                                     <  5:340307/136‑1 (MQ=255)         ttCCAGGGCTTGGCCCAGGGCGATTTCCATGATCTCTACGGTGATGCCGTTGTTCTTGATGTCCATCTGCAGAGCGGTGACGCATTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGTGGACTTCGTCa                                                                                                                    >  6:152566/1‑136 (MQ=255)           ccAGGGCTTGGCCCAGGGCGATTTCCATGATCTCTTCGGTGATGCCGTTGATCTTGATGTCCATCTGCAGCGCGGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCAcc                                                                                                                  >  4:148767/1‑136 (MQ=255)           ccAGGGCTTGGACCAGGGCGATTTCCATGATCTCTTCGGTGATGCCGTTGATCTTGATGTCCATCTGCAGCGCGGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTGGtagcc                                                                                                                  >  1:210502/1‑132 (MQ=255)             aGGGCTTGGCCCAGGGCGATTTCCATTATATCTTCGGTGATGTGGTTGATCTTGTTGTTCATCTGCAGCTCGGTGACGCGTTTGGCGTTACCGTCTACCTTGAAGTCGATGTCGCCCGGGTGGTCTTCGTCACCCa                                                                                                                <  2:22651/136‑1 (MQ=255)             aGGGCGTGGCCCAGGGCGATTTCCATGATCTCTTCGGTGATGCCGTTGATCTTGATGTCCATCTGCAGCGCGGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCACCCa                                                                                                                <  8:200091/136‑1 (MQ=255)               ggCTTGGCCCAGGGCGATTTCCATGTTCTCTTCGGTGATGCCGTTGATCTTGATGTCCATCTGCAGCGCGGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCACCCAgg                                                                                                              <  6:237244/136‑1 (MQ=255)               ggCTTGGCCCAGGGCGATTTCCATGATCTCTTCGGTGATGCCGTTGATCTTGATGTCCATCTGCAGCGCGGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCACCCAgg                                                                                                              <  7:161028/136‑1 (MQ=255)               ggCTTGGCCCAGGGCGATTTCCATGATCTCTTCGGTGATGCCGTTGATCTTGATGTCCATCTGCAGCGCGGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCACCCAgg                                                                                                              <  4:11193/136‑1 (MQ=255)               ggCTTGGCCCAGGGCGATTTCCATGATCTCTTCGGTGATGCCGTTGATCTTGATGTCCATCTGCAGCGCGGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCACCCAgg                                                                                                              <  6:206554/136‑1 (MQ=255)               ggCTTGGCCCAGGGCGATTTCCATGATCTCTTCGGTGATGCCGTTGATCTTGATGTCCATCTGCAGCGCGGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCACCCAgg                                                                                                              <  8:287469/136‑1 (MQ=255)                      cccAGGGCGATTTCCATGATCTCTTCGGTGATGCCGTTGATCTTGATGTCCATCTGCAGCGCGGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCACCCAGGATgtcag                                                                                                       >  8:168794/1‑136 (MQ=255)                      cccAGGGCGATTTCCATGATCTCTTCGGTGATGCCGTTGATCTTGATGTCCATCTGCAGCGCGGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCACCCAGGATgtcag                                                                                                       >  2:222916/1‑136 (MQ=255)                      cccAGGGCGATTTCCATGATCTCTTCGGTGATGCCGTTGATCTTGATGTCCATCTGCAGCGCGGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCACCCAGGATgtcag                                                                                                       >  8:211103/1‑136 (MQ=255)                      cccAGGGCGATTTCCATGATCTCTTCGGTGATGCCGTTGATCTTGATGTCCATCTGCAGCGCGGTGACGCCAAAGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGAGGTCTTCGTCACCCAGGAAgtcag                                                                                                       >  7:128748/1‑136 (MQ=255)                              gATTTCCATGATCTCTTCGGTGATGCCGTTGATCTTGATGTCCATCTGCAGCGCGGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCACCCAGGATGTCAGTCAGGACt                                                                                               >  8:127715/1‑136 (MQ=255)                              gATTTCCATGATCTCTTCGGTGATGCCGTTGATCTTGATGTCCATCTGCAGCGCGGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCACCCAGGATGTCAGTCAGGACt                                                                                               <  3:262353/136‑1 (MQ=255)                                   ccATGATCTCTTCGGTGATGCCGTTGATCTTGATGTCCATCTGCAGCGCGGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCACCCAGGATGTCAGTCAGGACTGCGa                                                                                           <  3:331921/135‑1 (MQ=255)                                        aTCTCTTCGGTGATGCCGTTGATCTTGATGTCCATCTGCAGCGCGGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCACCCAGGATGTCAGTCAGGACTGCGAACTTGt                                                                                     >  8:226524/1‑136 (MQ=255)                                             ttCGGTGATGCCGTTGATCTTGATGTCCATCTGCAGCGCGGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCACCCAGGATGTCAGTCAGGACTGCGAACTTGt                                                                                     <  3:294234/131‑1 (MQ=255)                                             ttCGGTGATGCCGTTGATCTTGATGTCCATCTGCAGCGCGGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCACCCAGGATGTCAGTCAGGACTGCGAACTTGt                                                                                     <  5:174842/131‑1 (MQ=255)                                             ttCGGTGATGCCGTTGATCTTGATGTCCATCTGCAGCGCGGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCACCCAGGATGTCAGTCAGGACTGCGAACTTGt                                                                                     <  2:41248/131‑1 (MQ=255)                                             ttCGGTGATGCCGTTGATCTTGATGTCCATCTGCAGCGCGGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCACCCAGGATGTCAGTCAGGACTGCGAACTTGt                                                                                     <  1:59738/131‑1 (MQ=255)                                             ttCGGTGATGCCGTTGATCTTGATGTCCATCTGCAGCGCGGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCACCCAGGATGTCAGTCAGGACTGCGAACTTGTCGcct                                                                                <  2:327889/136‑1 (MQ=255)                                             ttCGGTGATGCCGTTGATCTTGATGTCCATCTGCAGCGCGGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGGGGTCTTCGTCACCCAGGATGTCAGTGAGGACTGCGAACTTGt                                                                                     <  3:15594/131‑1 (MQ=255)                                               cGGTGATGCCGTTGATCTTGATGTCCATCTGCAGCGCGGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCACCCAGGATGTCAGTCAGGACTGCGAACTTGTCGcct                                                                                <  7:127715/134‑1 (MQ=255)                                                ggTGATGCCGTTGATCTTGATGTCCATCTGCAGCGCGGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCACCCAGGATGTCAGTCAGGACTGCGAACTTGTCGccttcc                                                                             >  3:254352/1‑136 (MQ=255)                                                  tGATGCCGTTTATCTTGATGTCCATCTGCAGCGCGGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCACCCAGGATGTCAGTCAGGACTGCGAACTTGTCGccttcctt                                                                           <  2:340539/136‑1 (MQ=255)                                                  tGATGCCGTTGATCTTGATGTCCATCTGCAGCGCGGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCACCCAGGATGTCAGTCATTACTTATAACTTGTCGccttcctt                                                                           <  1:61799/136‑1 (MQ=255)                                                  tGATGCCGTTGATCTTGATGTCCATCTGCAGCGCGGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCACCCAGGATGTCAGTCAGGACTGCGAACTTGTCGccttcctt                                                                           <  8:245960/136‑1 (MQ=255)                                                  tGATGCCGTTGATCTTGATGTCCATCTGCAGCGCGGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCACCCAGGATGTCAGTCAGGACTGCGAACTTGTCGccttcctt                                                                           <  2:45388/136‑1 (MQ=255)                                                  tGATGCCGTTGATCTTGATGTCCATCTGCAGCGCGGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCACCCAGGATGTCAGTCAGGACTGCGAACTTGTCGccttcctt                                                                           <  1:341683/136‑1 (MQ=255)                                                          ttGATCTTGATGTCCATCTGCAGCGCGGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCACCCAGGATGTCAGTCAGGACTGCGAACTTGTCTCCTTCCTTGACCAGGc                                                                   >  8:90171/1‑136 (MQ=255)                                                             aTCTTGATGTCCATCTGCAGCGCGGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGACCGGGTGGTCTTCCTCACCCAGGATGTCAGTCAGGACTGCGAACTTGTCGCCATCCTTGACCAGcccc                                                                 >  4:286083/1‑135 (MQ=255)                                                               cTTGATGTCCATCTGCAGCGCGGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCACCCAGGATGTCAGTCAGGACTGCGAACTTGTCGCCTTCCTTGACCAGGCACATg                                                              >  5:290460/1‑136 (MQ=255)                                                                  gATGTCCATCTGCAGCGCGGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCACCCAGGATGTCAGTCAGGACTGCGAACTTGTCGCCTTCCTTGACCAGGCCCATGGc                                                            >  7:80168/1‑135 (MQ=255)                                                                     gTCCATCTGCAGCGCGGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCACCCAGGATGTCAGTCAGGACTGCGAACTTGTCGCATTCCTTGACCAGGCCCATGGCGATa                                                        >  1:151300/1‑136 (MQ=255)                                                                              cAGCGCGGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCACCCAGGATGTCAGTCAGGACTGCGAACTTGTCGCCTTCCTTGACCAGGCCCATGGCGATACCGGCAAc                                                <  8:80168/135‑1 (MQ=255)                                                                                   cgGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCACCCAGGATGTCAGTCAGGACTGCGATCTTGTCGCCTTCCTTGACCAGGCCCATGGCGATACCGGCACCCGgcgc                                          >  5:266826/1‑136 (MQ=255)                                                                                      tGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCACCCAGGATGTCAGTCAGGACTGCGAACTTGTCGCCTTCCTTGACCAGGCCCATGGCGATACCGGCAACCGGCGCCtt                                       <  3:66980/136‑1 (MQ=255)                                                                                      tGACGCCTTTGGCGGGACCGGCTACCTTGAAGTCCAGGTCGCCCGGGTGGTCTTCGTCACCCAGGATGTCAGTCAGGACTTAGATCTTGTCGCCTTCCTTGACCAGGCCCATGGCGATACCGGCAACCGGCGCCtt                                       <  3:4625/136‑1 (MQ=255)                                                                                      ggACGCCGTTGGCGGTACCGGCTATCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCACCCAGGATGTCAGTCAGGACTGCGAACTTGTCGCCTTCCTTGACCAGGCCCATGGCGATACCGGCAACCGGCGCCtt                                       <  1:281272/135‑1 (MQ=255)                                                                                         cGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCACCCAGGATGTCAGTCAGGACTGCGAACTTGTCGCCTTCCTTGACCAGGCCCATGGCGATACCGGCAACCGGCGCCTTCAt                                    <  7:231940/136‑1 (MQ=255)                                                                                         cGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCACCCAGGATGTCAGTCAGGACTGCGAACTTGTCGCCTTCCTTGACCAGGCCCATGGCGATACCGGCAACCGGCGCCTTCAt                                    >  4:145170/1‑136 (MQ=255)                                                                                                        cGGCTACCTTGAAGTCCATGGCGCCCGGGTGGTCTTCGTCACCGAGGATGTCAGTCAGGACTGCGAACTTGTCGCCTTCCTTGACCAGGCCCATGGCGATACCGGCAACCGGCGCCTTCATCGGCACACCGGCGTc                     <  7:224956/136‑1 (MQ=255)                                                                                                         ggCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCACCCAGGATGTCAGTCAGGACTGCGAACTTg                                                                                      <  7:8812/70‑1 (MQ=255)                                                                                                         ggCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCACCCAGGATGTCAGTCAGGACTGCGAACTTg                                                                                      >  8:8812/1‑70 (MQ=255)                                                                                                         ggCTACCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCACCCAGGATGTCAGTCAGGACTGCGAACTTGTCGCCTTCCTTGACCAGGCCCATGGCGATACCGGCAACCGGCGCCTTCATCGGCACACCGGCGTcc                    <  5:292215/136‑1 (MQ=255)                                                                                                             aCCTTGAAGTCCATGTCGCCCGGGTGGTCTTCGTCACCCAGGATGTCAGTCAGGACTGCGAACTTGTCGCCTTCCTTGACCAGGCCCATGGCGATACCGGCAACCGGCGCCTTCATCGGCACCCCGCCGTCCAt                  >  6:332076/1‑134 (MQ=255)                                                                                                                      tCCATGTCGCCCGGGTGGTCTTCGTCACCCAGGATGTCAGTCAGGACTGCGAACTTGTCGCCTTCCTTGACCAGGCCCATGGCGATACCGGCAACCGGCGCCTTCATCGGCACACCGGCGTCCATCAGGGCCAggg       <  5:274015/136‑1 (MQ=255)                                                                                                                            tCGCCCGGGTGGTCTTCGTCACCCAGGATGTCAGTCAGGACTGCGAACTTGTCGCCTTCCTTGACCAGGCCCATGGCGATACCGGCAACCGGCGCCTTCATCGGCACACCGGCGTCCATCAGGGCCAGGGAAGCa  <  3:124560/135‑1 (MQ=255)                                                                                                                                  |                                                                                                                                 AGGCGCGCTTCCAGGGCTTGGCCCAGGGCGATTTCCATGATCTCTTCGGTGATGCCGTTGATCTTGATGTCCATCTGCAGCGCGGTGACGCCTTTGGCGGTACCGGCTACCTTGAAGTCCATGTCGCCCAGGTGGTCTTCGTCACCCAGGATGTCAGTCAGGACTGCGAACTTGTCGCCTTCCTTGACCAGGCCCATGGCGATACCGGCAACCGGCGCCTTCATCGGCACACCGGCGTCCATCAGGGCCAGGGAAGCA  >  ACB143/5345841‑5346098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑