BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB143	5,386,519	G→A	intergenic (‑528/+1123)	CJ019_05070 ← / ← nptII	hypothetical protein/neomycin phosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	5,386,519	0	G	A	100.0%	68.0 / NA	21	intergenic (‑528/+1123)	CJ019_05070/nptII	hypothetical protein/neomycin phosphotransferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (7/14); total (7/14)

ATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTC‑‑CGT‑‑CGT‑CA‑GGATC‑‑A‑‑TCCGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGG  >  ACB143/5386422‑5386604

aTAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTC‑‑AGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGAt                                                      <  7:26370/135‑1 (MQ=255)

       aaaTTGATCGAATTCTTTCATTTACGACCCTAATATGACATCTCGGCCGGCTGGTCGACGACGGCGGGCTC‑‑CGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTcc                                              <  7:167457/136‑1 (MQ=255)

       aaaTTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTC‑‑AGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTcc                                              <  1:119058/136‑1 (MQ=255)

         aTTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCGCGGCCGGCTTGTCGACGACGGCGGGCTC‑‑AGTGGGGT‑AC‑GGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgg                                            <  5:68628/136‑1 (MQ=255)

             aTCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTC‑‑AGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggggg                                         <  3:171180/135‑1 (MQ=255)

                        tCATTTACGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTC‑‑AGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTAtt                               <  8:196016/134‑1 (MQ=255)

                          aTTTAAGACCCTAATATGACATCTCGGCCGGCTTGGCGTCGACGGCGGTCTCATTGG‑‑GGT‑AC‑GGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCGCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcgc                           <  4:211001/136‑1 (MQ=255)

                          aTTAAACACCCTAATATGACAGCTCGGCCGGCTGGGCGACGACGGCGGGCTCAGGGG‑‑GGT‑AC‑GGCAC‑‑AACCCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcgc                           <  6:211020/136‑1 (MQ=255)

                           tttAAGACCCTAATATGACATCTCGGCCGGCGTGGCGACGACGGCGGTCTC‑‑AGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCt                          <  6:102944/136‑1 (MQ=255)

                             tAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTC‑‑AGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTg                         <  8:102916/135‑1 (MQ=255)

                                 aCCCAAACATGACCTCTCGGCCGGCTGGCCGACGACGGCGGTCTC‑‑CGT‑‑GGTGTACGGAACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGt                    <  5:206403/136‑1 (MQ=255)

                                       atatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTC‑‑AGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg              <  1:252428/136‑1 (MQ=255)

                                         atGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTC‑‑AGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGc            >  7:127590/1‑136 (MQ=255)

                                         atGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTC‑‑AGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGc            >  2:266125/1‑136 (MQ=255)

                                          tGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTC‑‑AGTGGTGT‑CC‑GGCACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGc            <  1:258104/135‑1 (MQ=255)

                                                ctcGGCCGGCTTGTCGACGACGGCGGTCTC‑‑AGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCt     >  4:73429/1‑136 (MQ=255)

                                                ctcGGCCGGCTTGTCGACGACGGCGGTCTC‑‑AGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCt     >  7:326557/1‑136 (MQ=255)

                                                 tcGGCCGGCTTGTCGACGACGGCGGTCTC‑‑AGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt    >  8:189126/1‑136 (MQ=255)

                                                   ggccggcTTGTCGACGACGGCGGTCTC‑‑AGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTgg  >  4:323933/1‑136 (MQ=255)

                                                   ggccggcTTGTCGACGACGGCGGTCTC‑‑AGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTgg  >  3:319361/1‑136 (MQ=255)

                                                   ggccggcGTGTCGACGACGGCGGTCTC‑‑AGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGccc      <  7:14793/132‑1 (MQ=255)

ATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTC‑‑CGT‑‑CGT‑CA‑GGATC‑‑A‑‑TCCGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGG  >  ACB143/5386422‑5386604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`