| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | ACB143 | 5,386,507 | +C | intergenic (‑516/+1135) | CJ019_05070 ← / ← nptII | hypothetical protein/neomycin phosphotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 5,386,507 | 1 | . | C | 95.2% | 67.5 / ‑2.6 | 21 | intergenic (‑516/+1135) | CJ019_05070/nptII | hypothetical protein/neomycin phosphotransferase |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (5/15); total (5/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01 | |||||||||||
TATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGT‑‑CGT‑C‑A‑GGATC‑‑ATCCGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGGCTCC > ACB143/5386417‑5386609 | tATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGT‑ACGGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGaa < 5:248219/137‑1 (MQ=255) aTAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGT‑ACGGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCa > 6:53047/1‑137 (MQ=255) aaCAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGT‑ACGGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCAcc > 3:272699/1‑137 (MQ=255) aaTTGATCTAATTCTTTCATTAAAGACCCAAATATGACATCTCGGCCGGCTTGGCGACGACGGCGGTCTCAGT‑‑GGTGT‑ACGGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgg < 5:253052/137‑1 (MQ=255) tGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGT‑ACGGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggggg < 1:118112/137‑1 (MQ=255) cGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGT‑ACGGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCttt > 5:99973/1‑137 (MQ=255) taaTTCTTTCATTTATGACCCTAATATGCCATCTCGGCCGGCTGGTCTACGACGGCGGTCTCAGT‑‑GGG‑CTACGGTACAAACCCCGACGCTAGCAACGCATGAGAAACCCCCCGGAAGATCACCTTCCTGGGGCtttt < 4:247285/136‑1 (MQ=255) aaTTCTTTCATTTACCACCATACAATTACACCTCGGCCGGCTTGGCGACGACGGGGGTCTCAGT‑‑GGTGT‑ACGGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCttttt < 4:215125/137‑1 (MQ=255) ttCATTTACGACCCAAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGGGT‑A‑C‑GGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgc < 8:250365/137‑1 (MQ=255) tCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGT‑ACGGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcg < 6:99973/137‑1 (MQ=255) aaTTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCATT‑‑GGTGT‑ACGGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcgc < 1:231046/136‑1 (MQ=255) aaTTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGT‑ACGGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcgc < 6:112873/136‑1 (MQ=255) aaTATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGT‑ACGGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg < 4:141415/137‑1 (MQ=255) atatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGT‑ACGGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCtt > 4:319199/1‑102 (MQ=255) atatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGT‑ACGGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCtt < 3:319199/102‑1 (MQ=255) tatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGT‑ACGGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGc < 6:157243/137‑1 (MQ=255) atGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGT‑ACGGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCg > 4:48497/1‑137 (MQ=255) gACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGT‑ACGGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTg < 4:184590/137‑1 (MQ=255) aCATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGT‑ACGGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGttc < 5:53047/137‑3 (MQ=255) ccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTGT‑ACGGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGACt < 5:189182/137‑1 (MQ=255) ggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTGT‑ACGGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGGCTcc < 4:272699/137‑1 (MQ=255) | TATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGT‑‑CGT‑C‑A‑GGATC‑‑ATCCGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGGCTCC > ACB143/5386417‑5386609 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |