New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	ACB143	= 4759431	78 (1.300)	3 (0.050)	3/258	7.0	4.3%	intergenic (+46/‑94)	fpvA_3/ligM_KToptimized	Ferripyoverdine receptor/sequence generated by OPTIMIZER for KT2440
	?	ACB143	4760973 =	56 (0.940)					intergenic (+33/+22)	ligM_KToptimized/lip2	sequence generated by OPTIMIZER for KT2440/Lipase 2
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CCAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  ACB143/4759295‑4759431 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGC  >  ACB143/4760973‑4761070                                                                                                                                                                                                                                             CCAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTT                                                                                                    <  3:226407/137‑1  CAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTt                                                                                                   >  6:206250/1‑136       CATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGt                                                                                              >  2:22504/1‑136                                                                                      TTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGAT               >  3:92432/1‑137                                                                                                   ACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGC  <  4:92432/137‑1                                                                                                                                                                                                                                             CCAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  ACB143/4759295‑4759431 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGC  >  ACB143/4760973‑4761070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.