Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	1,175,559	0	G	T	37.1%	86.8 / 13.2	62	P51P (CCG→CCT)	tadA	tRNA‑specific adenosine deaminase
Reads supporting (aligned to +/- strand):  ref base G (28/11);  new base T (0/23);  total (28/34)
Fisher's exact test for biased strand distribution p-value = 5.01e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATCGCAGTCGCGATCAGGAATTCATGCGCCTGGCGCTGGCCTTGGCCGCCGAAGGCGCGGCGTTGGGCGAGGTGCCAGTGGGGGCGGTGCTGGTGCAGCATGGGCAGGTGATCGGCCAGGGTTTCAA‑CCGG‑CCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTGGAACCGTGCAGCATGTGTGCG  >  ACB143/1175426‑1175688                                                                                                                                        |                                                                                                                                  atcgCAGTCGCGATCAGGAATTCATGCGCCTGGCGCTGGCCTTGGCCGCCGAAGGCGCGGCGTTGGGCGAGGTGCCAGTGGGGGCGGTGCTGGTGCAGCATGGGCAGGTGATCGGCCAGGGTTTCAA‑CCGG‑CCGatc                                                                                                                                >  2:18479/1‑137 (MQ=255)  tcgCAGTCGCGATCAGGAATTCATGCGCCTGGCGCTGGCCTTGGCCGCCGAAGGCGCGGCGTTGGGCGAGGTGCCAGTGGGGGCGGTGCTGGTGCAGCATGGGCAGGTGATCGGCCAGGGTTTCAA‑CCGG‑CCGatca                                                                                                                               >  8:181649/1‑137 (MQ=255)    gCAGTCGCGATCAGGAATTCATGCGCCTGGCGCTGGCCTTGGCCGCCGAAGGCGCGGCGTTGGGCGAGGTGCCAGTGGGGGCGGTGCTGGTGCAGCATGGGCAGGTGATCGGCCAGGGTTTCAA‑CCGG‑CCGatcctc                                                                                                                             >  8:307909/1‑137 (MQ=255)       gTCGCGATCAGGAATTCATGCGCCTGGCGCTGGCCTTGGCCGCCGAAGGCGCGGCGTTGGGCGAGGTGCCAGTGGGGGCGGTGCTGGTGCAGCATGGGCAGGTGATCGGCCAGGGTTTCAA‑CCGG‑CCGATCATCGAc                                                                                                                          >  8:335518/1‑137 (MQ=255)           cgATCAGGAATTCATGCGCCTGGCGCTGGCCTTGGCCGCCGAAGGCGCGGCGTTGGGCGAGGTGCCAGTGGGGGCGGTGCTGGTGCAGCATGGGCAGGTGATCGGCCAGGGTTTCAA‑CCGG‑CCGATCATCGACAGCg                                                                                                                      >  1:300931/1‑137 (MQ=255)           cgATCAGGAATTCATGCGCCTGGCGCTGGCCTTGGCCGCCGAAGGCGCGGCGTTGGGCGAGGTGCCAGTGGGGGCGGTGCTGGTGCAGCATGGGCAGGTGATCGGCCAGGGTTTCAA‑CCGG‑CCGATCATCGACAGCg                                                                                                                      >  5:230247/1‑137 (MQ=255)                   aaTTCATGCGCCTGGCGCTGGCCTTGGCCGCCGAAGGCGCGGCGTTGGGCGAGGTGCCAGTGGGGGCGGTGCTGGTGCAGCATGGGCAGGTGATCGGCCAGGGTTTCAA‑CCGG‑CCGATCATCGACAGCGACCCCAgc                                                                                                              >  6:88983/1‑137 (MQ=255)                             ccgggggcTGGCCTGGGCGGCCGACGGGGGGGGGTTGGGCGCGGGCCCCGTGGGGGCGGGGCTGGTGCAGCTTGGCCTGTTGATCGCCCTGGGTTTCAA‑CCGC‑CCTATAATCGACAGCGACCCCAGCGCGCATGCCg                                                                                                    <  4:102414/131‑1 (MQ=255)                                ggCGCTGGCCTTGGCCGCCGAAGGCGCGGCGTTGGGCGAGGTGCCAGTGGGGGCGGTGCTGGTGCAGCATGGGCAGGTGATCGGCCAGGGTTTCAA‑CCGG‑CCGATCATCGACAGCGACCCCAGCGCGCATGCCgaga                                                                                                 >  7:68518/1‑137 (MQ=255)                                  gggtGGCGTGGGCGGCCTATGGGGGGGCGTTGGGCGAGGTGCCTGGGGGGGCGGTGGGGGTGCAGCTTGGGCTGTTTAGCGGCCTGGTTTTCAA‑CCGG‑CCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGAtg                                                                                               <  6:254878/134‑1 (MQ=255)                                   gcTGGCCTTGGCCGCCGAAGGCGCGGCGTTGGGCGAGGTGCCAGTGGGGGCGGTGCTGGTGCAGCATGGGCAGGTGATCGGCCAGGGTTTCAA‑CCGG‑CCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGAtgg                                                                                              >  5:5764/1‑137 (MQ=255)                                    cTGGCCTTGGCCGCCGAAGGCGCGGCGTTGGGCGAGGTGCCAGTGGGGGCGGTGCTGGTGCAGCAGGGGCAGGTGATCGGCCAGGGTTTCAA‑CCGG‑CCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGAtggt                                                                                             >  6:225876/1‑137 (MQ=255)                                         cTTGGCCGCCGAAGGCGCGGCGTTGGGCGAGGTGCCAGTGGGGGCGGTGCTGGTGCAGCATGGGCAGGTGATCGGCCAGGGTTTCAA‑CCGG‑CCGATCATCGACAGCGACCCCAGCGAGCATGCCGAGATGGTGGCGa                                                                                        >  6:320464/1‑137 (MQ=255)                                               cgccgAAGGCGCGGCGTTGGGCGAGGTGCCAGTGGGGGCGGTGCTGGTGCAGCATGGGCAGGTGATCGGCCAGGGTTTCAA‑CCGG‑CCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAggg                                                                                  >  2:261851/1‑137 (MQ=255)                                               cgccgAAGGCGCGGCGTTGGGCGAGGTGCCAGTGGGGGCGGTGCTGGTGCAGCATGGGCAGGTGATCGGCCAGGGTTTCAA‑CCGG‑CCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAggg                                                                                  >  6:375725/1‑137 (MQ=255)                                               cgccgAAGGCGCGGCGTTGGGCGAGGTGCCAGTGGGGGCGGTGCTGGTGCAGCATGGGCAGGTGATCGGCCAGGGTTTCAA‑CCGG‑CCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAggg                                                                                  >  2:129376/1‑137 (MQ=255)                                                       gcgcGGCTCGGGGCGCGGGGCCCGGGGGGGCGGTGCGGGGGCAGCAGGGGCTGGTCAGCGCCCGGGGTTCCAC‑CCGG‑CCGAACATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGcc                                                                          <  4:21611/137‑1 (MQ=255)                                                              gTTGGGCGAGGTGCCAGTGGGGGCGGTGCTGGTGCAGCATGGGCAGGTGATCGGCCAGGGTTTCAA‑CCGG‑CCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCgg                                                                   >  7:220863/1‑137 (MQ=255)                                                                          gcatgtgggtggGGTGCTGTTGCTGCTTGGGCTGGTGAACGCCTTGGGTTTCTA‑CCGG‑CCTTTCATCGACAGCGACCCCAGCGCGCATGCCGAGAGGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTAcc                                                       <  6:335574/133‑1 (MQ=255)                                                                          gCCAGTGGGGGCGGTGCTGGTGCAGCATGGGCAGGTGATCGGCCAGGGTTTCAA‑CCGG‑CCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTAcc                                                       >  4:127960/1‑137 (MQ=255)                                                                          gCCAGTGGGGGCGGTGCTGGTGCAGCATGGGCAGGTGATCGGCCAGGGTTTCAA‑CCGG‑CCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTAcc                                                       >  6:376988/1‑137 (MQ=255)                                                                          gCCAGTGGGGGCGGTGCTGGTGCAGCATGGGCAGGTGATCGGCCAGGGTTTCAA‑CCGG‑CCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTAcc                                                       >  5:291930/1‑137 (MQ=255)                                                                            ccgggggggCGGGGCGGGGCCAGCATGGGCTGGTGAGCGGCCTGGGGTTCAA‑CCGG‑CCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCgg                                                     <  4:268113/133‑1 (MQ=255)                                                                              gggggggCGGGGCTGGTGGAGGAGGGGATGGTCTGGGGCGTGGGTTTAAA‑CGGG‑CCTATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCt                                                   <  7:189194/135‑1 (MQ=255)                                                                                    ggggTGCGGGTGCCGCAGGGGCCGGTGAGCGGCAAGGGTTACAC‑CGGG‑CCTAAAATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCgg                                             <  5:320464/135‑1 (MQ=255)                                                                                    ggggTGCGGGTGCAGCATGGGCTGGTGAGCGGCCAGGGTTTCAA‑CCGG‑CCTATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCgg                                             <  1:18479/135‑1 (MQ=255)                                                                                    gcggggcgggGGCACCATGGGCAGGTCATCGGCCAGGGTTTCAC‑CCGG‑CCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCAgg                                             <  6:283947/137‑1 (MQ=255)                                                                                    gCGGTGCTGGTGCAGCATGGGCAGGTGAGCGGCCAGGGTTTCAC‑CCGG‑CCTATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCgg                                             <  5:88983/137‑1 (MQ=255)                                                                                     cGGTGCTGGTGCAGCATGGGCAGGTGATCGGCCAGGGTTTCAA‑CCGG‑CCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGAGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGgc                                            >  3:13601/1‑137 (MQ=255)                                                                                      ggggcgggTGCACCAGTGGCTGGTGATCGGCCTGGTTATCAC‑CCGC‑CCTATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGgca                                           <  8:349228/131‑1 (MQ=255)                                                                                      ggTGCTGGTCCTGCAGGGGCTGGTCTGCGCCCTGTTTTTCAA‑CCGG‑CCTATCCTCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGgca                                           <  7:16838/137‑1 (MQ=255)                                                                                        gggtGGGGCAGCTTGGGCCGGTGAGCGCCCCGGGTGTCAC‑CCGG‑CCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGgcagc                                         <  3:73991/134‑1 (MQ=255)                                                                                        ggCTGGTGCTGCTGGGGCAGGTTTGCGGCCCGGGTTTCAC‑CCGG‑CCTATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGgcagc                                         <  2:96795/136‑1 (MQ=255)                                                                                         gCTGGTGCAGCAGGGGCAGGTGAGCGGCCTGGGTTTCAC‑CCGG‑CCTATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGgcagca                                        <  7:181649/137‑1 (MQ=255)                                                                                           tGGTGCAGCATGGGCAGGTGATCGGCCAGGGTTTCAA‑CCGG‑CCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCAcc                                      >  6:78906/1‑137 (MQ=255)                                                                                                cagctggggCTGGTTAGCGCCCCGGTTTGCAA‑CGGG‑CCTATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTa                                 <  3:54850/131‑1 (MQ=255)                                                                                                cagctTGGGCTGGTTATCGCCCAGGGTTTAAA‑CGGG‑GCTTTCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTa                                 <  5:141530/137‑1 (MQ=255)                                                                                                cagcaTGGGCTGGTGATCGGCCAGGGTTTCAA‑CCGG‑CCTATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTa                                 <  6:125140/137‑1 (MQ=255)                                                                                                cagaaTGGGCTGGTGAGCGGCAAGGGTTTCAA‑CCGG‑CCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTa                                 <  2:5936/137‑1 (MQ=255)                                                                                                aagattGGGCAGGTGATCGGCCTGGTTTTCAA‑CGGG‑CGTATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTa                                 <  8:125044/132‑1 (MQ=255)                                                                                                aagatggggCGGGTGAGCGGCCTGGGTTACAC‑CGGG‑CGTATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTa                                 <  4:351970/131‑1 (MQ=255)                                                                                                     tGGGCAGGTGATCGGCCAGGGTTTCAA‑CCGG‑CCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTAtgtgc                            >  3:188226/1‑136 (MQ=255)                                                                                                     tGGGCAGGTGATCGGCCAGGGTTTCAA‑CCGG‑CCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGa                            >  6:299733/1‑137 (MQ=255)                                                                                                     tGGGCAGGTGATCGGCCAGGGTTTCAA‑CCGG‑CCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGa                            >  3:351970/1‑137 (MQ=255)                                                                                                           gttGAGCGGCCTGGGTTTAAA‑CCGT‑CCTATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTgg                      <  8:183088/135‑1 (MQ=255)                                                                                                           ggtgcgcGGCCAGGTTTGCAC‑CGGG‑CCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTgg                      <  5:328415/131‑1 (MQ=255)                                                                                                           ggTGATCGGCCAGGGTTTCAA‑CCGG‑CCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTgg                      >  8:337249/1‑137 (MQ=255)                                                                                                           ggTGATCGGCAAGGGTTTCAC‑CGGG‑CCTATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTgg                      <  2:300931/137‑1 (MQ=255)                                                                                                               agcGGCCTGGGTTTCAA‑CCGG‑CCTATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTGGAAcc                  <  5:349363/135‑1 (MQ=255)                                                                                                               agcGGCCTGGGTTTCAA‑CCGG‑CCGTTCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTGGAAcc                  <  4:271203/135‑1 (MQ=255)                                                                                                               aTCGGCCTGGTTTTCAA‑CGGG‑CCTATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTGGAAcc                  <  5:335383/137‑1 (MQ=255)                                                                                                               aTCGGCCAGGGTTTCAA‑CCGG‑CCGATCATCGACAGCGCCCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTGGAAcc                  >  2:49255/1‑137 (MQ=255)                                                                                                               aTCGGCCAGGGTTTCAA‑CCGG‑CCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTGGAAcc                  >  1:244633/1‑137 (MQ=255)                                                                                                                 gggCCCGGGGTTAAC‑CCGG‑CTTATCCTCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTGGAACCGt                <  2:260634/136‑1 (MQ=255)                                                                                                                  ggCCAGGGTTTCAA‑CCGG‑CCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTGGAACCGTg               >  8:23502/1‑137 (MQ=255)                                                                                                                     cAGGGTTTCAA‑CCGG‑CCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGCCCCTGGAACCGTgcac            >  6:358157/1‑136 (MQ=255)                                                                                                                      ctggTTTCCA‑CCGGCCCTATAATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTGGAACCGTgcag            <  8:16697/135‑1 (MQ=255)                                                                                                                      ctggTTTAAACCCGG‑CCTAACATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTGGAACCGTgcag            <  5:244237/135‑1 (MQ=255)                                                                                                                          ttccAA‑CCGG‑CCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTGGAACCGTGCAGCAtgt       <  7:335518/134‑1 (MQ=255)                                                                                                                           ttCAA‑CCGG‑AGTATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTGGAACCGTGCAGCAtgtg      <  3:281115/137‑1 (MQ=255)                                                                                                                               a‑CCGG‑CCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTGGAACCGTGCAGCATGTGTGCg  <  5:376988/137‑1 (MQ=255)                                                                                                                               a‑CCGG‑CCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTGGAACCGTGCAGCATGTGTGCg  <  3:127960/137‑1 (MQ=255)                                                                                                                                        |                                                                                                                                  ATCGCAGTCGCGATCAGGAATTCATGCGCCTGGCGCTGGCCTTGGCCGCCGAAGGCGCGGCGTTGGGCGAGGTGCCAGTGGGGGCGGTGCTGGTGCAGCATGGGCAGGTGATCGGCCAGGGTTTCAA‑CCGG‑CCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTGGAACCGTGCAGCATGTGTGCG  >  ACB143/1175426‑1175688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑